93 results about "Acetobacter calcoaceticus" patented technology

The invention relates to acinetobacter calcoaceticus X.L.Zhu.SGD07-3-16 with free living nitrogen fixation capability. The acinetobacter calcoaceticus is preserved in China general microbiological culture collection center, and the preservation number of the acinetobacter calcoaceticus is CGMCCNo.5455. The acinetobacter calcoaceticus is high in nitrogenase activity and phosphorus and potassium dissolving capability, and has ACC (1-aminocyclopropane-1-carboxylic acid) deaminase activity, a certain amount of indoleacetic acid can be generated, and the generation of siderophore is avoided; when an acinetobacter calcoaceticus-containing liquid or solid microbial agent is used for inoculating crops such as corns, oilseed rapes and leaf mustard, the growth of the crops can be remarkably promoted, and the yield of the crops can be increased.

The invention relates to an Acinetobacter calcoaceticus WG-6 capable of degrading oil and a culture method and application thereof, and belongs to the technical field of microorganisms. The bacterial strain has been collected in a China General Microbiological Culture Collection Center on June 11th, 2010 with a collection number of CGMCC No.3915. The Acinetobacter calcoaceticus can directly decompose oil contaminants into a nutrient material for utilization and growth and reproduction, and has remarkable oil degradation effect. The invention also provides a high-density microbial inoculum prepared by mixing the Acinetobacter calcoaceticus and other components, the microbial inoculum can rapidly decompose the oil, and the other components in the microbial inoculum can improve the microecological environment of soil, and accelerate the soil mediation progress.

A fusion protein of pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) and a cytochrome is disclosed. PQQGDH is, for example, a water-soluble PQQGDH derived from Acinetobacter calcoaceticus. The cytochrome is, for example, an electron transfer domain of quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni. The fusion protein of the present invention shows intramolecular electron transfer from PQQ, a redox center, to the cytochrome, which allow construction of a direct electron transfer-type glucose sensor which requires no electron mediators.

The invention discloses a sedum alfredii endophyte and an application thereof. The endophyte is named as Acinetobacter calcoaceticus Sasm3 with a preservation number of CGMCC NO.7334. The invention also discloses an application of the endophyte in plant-microorganism combined remediation on heavy metal contaminated soil. The application comprises the steps of: inoculating sedum alfredii with the endophyte and planting the sedum alfredii inoculated with the endophyte in the heavy metal contaminated soil to be remedied; and from the second growing season of the sedum alfredii inoculated with the endophyte, re-inoculating the sedum alfredii with the endophyte in each growing season, and harvesting the sedum alfredii. The endophyte can be used for enhancing the resistance of the sedum alfredii to high-concentration heavy metal so as to promote the healthy growth of the sedum alfredii in the environment of high-concentration heavy metal, thereby increasing the biomass of the sedum alfredii and enhancing the remediation efficiency of the sedum alfredii to the heavy metal contaminated soil.

The invention discloses a heavy-metal-resisting microbial agent, a preparation method and an application thereof. The preparation method of the microbial agent comprises the following steps of: (1) respectively carrying out liquid culture in a fluid medium after activating acinetobacter calcoacetcus with the preservation number of CGMCC No.6820 and pseudomonas aeruginosa with the preservation number of CGMCC No.6821 to obtain bacterial liquid A and bacterial liquid B; and (2) inoculating the mixed bacterial liquid A and B to a solid fermentation medium, ventilating, stirring and fermenting for 24-48 hours at 28-38 DEG C, and drying in vacuum to obtain the heavy-metal-resisting microbial agent. The heavy-metal-resisting microbial agent disclosed by the invention can not only remove a part of heavy metal ions, but also can remove a major of organic pollutants.

The invention relates to a preparation method and an application of a petroleum degrading enzyme preparation. The preparation method comprises the following preparation steps: performing cell breakage on microorganisms for degrading petroleum to prepare crude enzyme liquid; performing mixed adsorption on the crude enzyme liquid and a carrier; and separating and drying to prepare the petroleum degrading enzyme preparation, wherein the microorganisms for degrading the petroleum are acinetobacter calcoaceticus; the collection number of the strains is CGMCC No.3915; and the collection mechanism is Common Microorganism Center of China General Microbiological Culture Collection Center. The invention also comprises the application of the prepared petroleum degrading enzyme preparation to petroleum degradation. An enzyme system of the microorganisms with a petroleum degrading function is fixed by an adsorbent and then is used for degrading soil polluted by the petroleum, so that the degradation efficiency is obviously improved and is increased by 30 to 50 times compared with the microbial degradation speed, and the stability is improved by 15 to 20 times compared with that of the crude enzyme liquid.

The invention discloses a fluorescent quantitative polymerase chain reaction (PCR) kit and method for an Acinetobacter baunannii / calcoaceticus complex OXA-23 mutant gene. Special oligonucleotide primers and probes are designed according to sequences of the mutant of the newly screened D-type beta-lactamase drug-resistant gene OXA-23 of the Acinetobacter baunannii / calcoaceticus complex. The fluorescent quantitative PCT kit can be used for detecting the Acinetobacter baunannii / calcoaceticus complex.

The invention relates to heterotrophic nitrification-aerobic denitrification composite microbial inoculum with salt tolerance and high ammonia nitrogen tolerance, preparation and application thereof.The composite microbial inoculum is prepared by compounding of Acinetobacter calcoaceticus TNJ-1, pseudomonas stutzeri, Halomonas denitrificans and Cupriavidus sp. SWA1. Through the synergistic actionof the four microorganisms, the effective treatment of high ammonia nitrogen wastewater can be realized under aerobic and high-salt conditions, and toxic substances such as chlorides, heavy metals and the like can be effectively resisted.

The invention provides a compound biological enzyme preparation, a compound microbial agent and application thereof in the treatment of oily sludge. The compound biological enzyme preparation is prepared from bacillus subtilis, bacillus pumilus, candida and lactobacillus brevis. The compound microbial agent is prepared from candida tropicalis, sphingobacterium multivolume, bacillus cereus, acinetobacter calcoaceticus and bacillus megaterium. The invention also provides the application of the compound biological enzyme preparation and / or the compound microbial agent in the treatment of oily sludge and a method for treating the oily sludge. The method comprises steps of treating the oily sludge with the compound biological enzyme preparation to form an upper oil phase, an intermediate aqueous phase and a lower sludge phase, and treating the lower sludge phase and / or the intermediate aqueous phase by using the composite microbial agent. The compound biological enzyme preparation, the compound microbial agent and the application thereof provided by the invention have the advantages of harmless treatment of oily sludge, low cost, simplicity and convenience, no secondary pollution, and broad application prospects.

The invention provides acinetobacter calcoaceticus capable of efficiently degrading lignin. The acinetobacter calcoaceticus is characterized in that the acinetobacter calcoaceticus LG-1 is registered and collected in the China Center for Type Culture Collection (CCTCC) on March 12, 2014 with CCTCC No. M2014079. The acinetobacter calcoaceticus has a high-efficiency degradation effect on lignin and can grow in a lignin inorganic salt culture solution taking lignin as only carbon source, and the growing curve chart of the acinetobacter calcoaceticus shows that the growing effect of the acinetobacter calcoaceticus is obviously higher than that of rhodococcus serving as positive control and that of escherichia coli serving as negative control; the bacterial strain can be applied to the fields such as papermaking, energy sources and environmental protection; and the problem of great difficulty in degradation of alkali lignin in all industries is solved by using the high-efficiency degradation efficiency of the acinetobacter calcoaceticus for the alkali lignin.

The invention relates to the field of treating environment by using microorganisms, in particular to acinetobacter calcoaceticus T32 capable of metabolizing furazolidone, and application thereof. The acinetobacter calcoaceticus T32 has been registered and collected in the China General Microbiological Culture Collection (CGMCC) on sixth, November, 2010; and the collection number is CGMCC No.4311. The acinetobacter calcoaceticus T32 can be used as bactericide for degrading the furazolidone. The furazolidone in the environment is removed by the metabolic process of T32, and the acinetobacter calcoaceticus T32 has the advantages of high degradation speed, low cost, convenience of operation and the like.

The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

The invention relates to a method for studying synergistic effect of surfactant producing bacteria on petroleum degradation. According to the method, a mineral culture medium for use in degradation experiments is prepared; and Pseudomonas aeruginosa GIMT1.074 is capable of producing surfactant in a mineral culture medium with glycerol as the only carbon source. Pseudomonas aeruginosa GIMT1.074 and acinetobacter calcoaceticus 061021B are inoculated with the initial OD600 being 0.01, and three groups of parallel tests are carried out respectively; according to the distinct phenotype of pseudomonas aeruginosa GIMT1.074 and acinetobacter calcoaceticus 061021B in a panel, the number of each kind of bacteria at corresponding moment is obtained by a plate count method, and the synergistic effect of pseudomonas aeruginosa GIMT1.074 and acinetobacter calcoaceticus 061021B in petroleum degradation is studied. The degradation rate of petroleum pollutants is improved by 12.83% due to the synergistic effect of the two strains.

A modified pyrroloquinoline quinone-dependent glucose dehydrogenase having a low reactivity with respect to maltose, galactose, etc. is provided. A modified pyrroloquinoline quinone-dependent glucose dehydrogenase including an amino acid sequence in which one or more amino acids in a region corresponding to a first region consisting of amino acids at positions 326 to 354 in pyrroloquinoline quinone-dependent glucose dehydrogenase derived from Acinetobacter calcoaceticus are substituted as compared with an amino acid sequence of the corresponding wild-type enzyme.

The invention provides a rhodobacter sphaeroides strain and a preparation method and application thereof. P-hydroxybenzoic acid transportprotein pcaK gene from acinetobacter calcoaceticus is introduced into the rhodobacter sphaeroides strain, the ability of taking p-hydroxybenzoic acid from an extracellular medium of the rhodobacter sphaeroides strain is enhanced, and therefore, the yield of synthesizing coenzyme Q10 in the rhodobacter sphaeroides strain is improved. By the rhodobacter sphaeroides strain, the shortcomings that in production of coenzyme Q10 by microbiological fermentation, the yield is low, the production cost is high and the like are overcome, the yield of the intracellular coenzyme Q10 of rhodobacter sphaeroides can be improved remarkably, and the cost of industrialized production of the coenzyme Q10 is reduced.

The invention discloses a strain of sewage treatment function bacterial. The sewage treatment function bacterial comprises the following five microbes: Bacillus cereus, Bnfillus licheniformis, Bacillus subtilis, Acinetobacter calcoaceticus and Paracoccus denitrificans. Cultivation of the microbes comprises the following processes: sampling from a target environment, culturing in a culture medium, separating target strains, and carrying out index analysis, species identifying, compatibility and optimizing on the separated strains. The function bacterial of the present invention has characteristics of rapid proliferation in an organic matter decomposition process, and significant phosphorus removal effect on sewage, such that a sewage treatment effect meets the national discharge standard, and sludge discharge can be substantially reduced.

The invention relates to a composite microbial inoculum for harmless processing, and its application. The composite microbial inoculum for harmless processing comprises Paracoccus denitrificans, Bacillus megaterium, Bacillus subtilis, Bacillus badius, Bacillus thuringiensis, Bacillus licheniformis, Acinetobacter calcoaceticus, Bacillus laterosporus and Bacillus cereus. The composite microbial inoculum can be used for processing sewage, domestic organic wastes, sewage plant sludge, or human and animal excreta.

The invention provides an aerobic microflora capable of quickly decomposing straw completely and belongs to the field of environmental protection and the technical field of microorganisms. According to the aerobic microflora capable of quickly decomposing straw completely, the aerobic functional microflora capable of quickly decomposing the straw is formed through screening and optimizing for the current situation that at present the straw is disorderly treated, and the aerobic functional microflora comprises bacillus subtilis, acinetobacter calcoaceticus, bacillus megatherium, alcaligenes faecalis, bacillus cereus and bacillus firmus. The invention provides a method capable of quickly decomposing the straw, that is, the aerobic microflora is added into the smashed straw; and the aerobic microflora can synergistically decompose straw organic solid waste substances through various clastic enzymes such as protease, lipase and cellulase which are generated by the aerobic microflora, and two thirds of the straw is decomposed within 24 h, and 100% softening of fibers is achieved.

The invention belongs to the technical field of microbes and discloses a composite fungicide for remediating lead-containing wastewater. The composite fungicide is prepared from a compound bacterium solution and an adsorption carrier and is characterized in that the compound bacterium solution is prepared from marsh rhodop seudanonas palustris, bacillus stearothermophilus, arthrobacter crystallopoietes, acinetobacter calcoaceticus, achromobacter xylosoxidans fermentation liquor and bacillus cereus. The composite fungicide can effectively remediate lead polluted wastewater, and sludge production amount is small.

The invention relates to acinetobacter calcoaceticus TA221 separated from an advanced plant. The strain can be used for producing homoserine lactone and controlling plant diseases, and belongs to the field of biotechnology. As proved by identification, the strain is an acinetobacter calcoaceticus strain, and is collected in the China General Microbiological Culture Collection Center with the collection number CGMCC No.4895. The strain can be well colonized on the surface of a plant in an application process, shows high stability and high continuity of control effects, and has a very good application prospect on the control of plant diseases.

The invention relates to a mixed microbial preparation for recovering soil polluted by composite pollutant oil in a biology manner and a manufacture method of the mixed microbial preparation. The mixed microbial preparation is made by mixing six bacterial strains including acinetobacter calcoaceticus, klebsiella pneumonia, enterobacter cancerogenus, pseudomonas stutzeri, rhodococcus erythropolis, and pseudomonas putida with one of rhodococcus, acinetobacter, gordonia, sphingobacterium, novosphingobium capsulatum, acinetobacter, and xanthomonas at a ratio of 1:1:2:2:1:1:1:1 and is used for recovering oil-polluted soil.

The invention relates to a preparation method and application of a sludge petroleum degrading complex enzyme. The preparation method comprises the following steps: (1), performing cell disruption on acinetobacter calcoaceticus, centrifuging, and taking a supernatant to obtain a petroleum degrading enzyme solution 21#; (2), mixing the petroleum degrading enzyme solution 21# and formate dehydrogenase to obtain the sludge petroleum degrading complex enzyme. For the first time, after a petroleum-degrading enzyme system derived from acinetobacter calcoaceticus is mixed with the formate dehydrogenase in proportion, the sludge petroleum degrading complex enzyme can be applied to degradation and repair of petroleum in high-concentration petroleum-contaminated sludge, so that immobilization of adsorbents such as diatomite is not required, and the petroleum in the sludge can be reduced in a short time. The sludge petroleum degrading complex enzyme has high-efficiency petroleum contamination treatment capacity, has low production cost, and has a wide application prospect.

The invention discloses acinetobacter calcoaceticus for promoting rhizosphere growth of walnuts. The acinetobacter calcoaceticus is named acinetobacter calcoaceticus (Acinetobacter calcoaceticus) X128 in class, and preserved at the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 31, 2012; and the preservation number is CGMCC No.7071. The invention also provides application of the acinetobacter calcoaceticus for promoting rhizosphere growth of the walnuts. The acinetobacter calcoaceticus for promoting rhizosphere growth of the walnuts is evenly fed to the periphery of the walnut root in a pouring manner; root growth can be obviously facilitated; the quantity of lateral roots is improved; and growth of the bottom diameter of the walnut and the height of seedling is facilitated. In contrast, the difference reaches a significant level. Therefore, excellent strain resources are provided for developing a special microbial fertilizer for the walnut in the future.

The present invention belongs to the technical field of soil environments, and specifically relates to a microbial soil improvement agent for petroleum polluted soil. The microbial soil improvement agent comprises the following raw materials: Paxillus involutus Hypha, Hebeloma mesophaeusm, Acinetobacter calcoaceticus, Sphingomonas paucimobilis, pyrene degrading bacteria, Glomus etunicatum, Cephalosporium roseum, Aureobasidium pullulans, nitrilase, long-chain alkane degradation enzyme, integration membrane di iron alkane hydroxylase, phenylalanine deaminase, arginine dihydrolase, Rhodococcus erythropolis, pseudomonas aeruginosa, sodium oleate, thiamine hydrochloride, calcium pantothenate, oyster shell powder, vermiculite powder, paspalum wettsteinii hackel powder, kentucky eupatorium fortunei powder, miscanthus floridulus powder, medicago sativa powder, pig manure granules, goat manure granules, gamma-polyglutamic acid, malic acid, citric acid, and Nostocales powder. According to the present invention, various types of the microorganisms in the improvement agent synergistically coordinate, and co-act with the carrier raw materials; and with the application of the improvement agent to treat different types of crude oil polluted soil in the Shengli oil field, the total removal rate of the petroleum hydrocarbon in the polluted soil achieves 77.68-89.76%.

Immobilized cell beads incorporating formulated microbial consortium comprising a synergistic mixture of the following bacterial strains namely, Enterobacter sakazaki, Pseudomonas aeruginosa and Aeromonas sobria selected from the following isolated bacterial strains namely, Yersinia enterocolitica, Aeromonas sobria, Klebsiella pneumoniae, Serratia liquefaciens, Enterobacter sakazaki, Citrobacter amalonaticus, Pseudomonas fluorescens, Pseudomonas aeruginosa, Enterobacter cloaca, Acinetobacter calcoaceticus are prepared, the formulated microbial consortium is immobilized in an appropriate immobilizing agent resulting in the formation of beads and the beads are used for instant BOD estimation using an electronic device and the formulated cell beads are reusable and are capable of assimilating most of the organic matter present in varied industrial effluents.

The invention discloses an agricultural compound microbial agent and application thereof, belonging to the technical field of microorganisms. The agricultural compound microbial agent comprises enterobacter aerogenes CT-B09-2, acinetobacter calcoaceticus WYS-A01-1, bacillus amyloliquefaciens JL-B05, bacillus amyloliquefaciens JL-B06 and bacillus licheniformis LYN-3, wherein the total viable bacteria concentration is (5-8)*10<8> cfu / ml or (2-3)*10<9> cfu / g. The agricultural compound microbial agent can promote the growth of crops, improves the disease-preventing, disease-resisting, stress-resisting and continuous-cropping-resisting capabilities of the crops, increases the yield of the crops and improves the quality of agricultural products.

Disclosed is a modified glucose dehydrogenase having pyrroloquinoline quinone as a coenzyme, wherein one or more amino acid residues in a region of amino acid 349-377 of water-soluble PQQGDH derived from Acinetobacter calcoaceticus is replaced with other amino acid residues and has an inhibition constant (Ksi) of 200 mM or more. The modified water-soluble PQQGDH of the invention can be utilized for measuring glucose levels in the presence of high concentrations of glucose because of the low substrate inhibition by glucose.

The invention relates to bio-organic fertilizer for preventing tomato autotoxicity. The bio-organic fertilizer is mainly prepared by fermenting, by mass, 45-55% of coal humic acid, 20-35% of corn meal, 5-10% of bran, 5-10% of volcanic stone powder, 1-3% of magnesium sulfate, 0.5-1% of zinc sulfate, 0.5-1% of manganese sulfate, 0.1-0.2% of brown sugar and 0.1-0.2% of fermentation microorganisms, wherein the fermentation microorganisms are bacillus subtilis, bacillus licheniformis, rhizopus oryzae and trichoderma viride, and total number of viable bacteria is greater than 5X108cfu / g; fermented fertilizer further contains a functional microorganism with total number of viable bacteria being greater than 2X107cfu / g, and the functional microorganism is acinetobacter calcoaceticus. The bio-organic fertilizer can effectively prevent tomato autotoxicity and improve tomato quality and yield.