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Composite microbial inoculum for harmless processing, and its application

A technology of harmless treatment and compound bacterial agent, applied in the field of microorganisms, can solve the problems of secondary pollution of dioxin environment and insignificant effect, and achieve the effect of reducing organic waste, reducing treatment cost and wide adaptability

Inactive Publication Date: 2013-02-13
WUXI ZHONGQUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, domestic organic waste, sludge, and human and animal manure are mainly disposed of by landfill or dumped into the sea. A few of them are disposed of by incineration, which also brings serious damage to the environment due to the generation of dioxins in the incineration. Secondary pollution
Some are treated with microbial methods, but the effect is not obvious

Method used

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  • Composite microbial inoculum for harmless processing, and its application
  • Composite microbial inoculum for harmless processing, and its application
  • Composite microbial inoculum for harmless processing, and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 composite bacterial agent

[0035] I Fermentation culture of various bacteria

[0036] Medium: LB

[0037] Culture conditions: Shake the flask at 37°C until the bacterial concentration is about 10 8 ~10 11 pieces / ml.

[0038] Inoculate the slant seeds of Paracoccus denitrificans, Bacillus megaterium, Bacillus subtilis, Bacillus chestnut, Bacillus thuringiensis, Bacillus licheniformis, Acinetobacter calcium acetate, Bacillus lateral sporosum and Bacillus cereus into LB liquid Culture medium, each bacteria were cultured separately.

[0039] II The configuration of the composite bacterial agent: get the liquid fermented liquid of each bacterium and mix according to the weight ratio in the table below to prepare the composite bacterial agent:

[0040]

[0041] Store at 4°C after the preparation of the compound bacterial agent is completed.

Embodiment 2

[0042] Embodiment 2 sewage treatment

[0043] Treatment object: swill sewage

[0044] Processing steps:

[0045] 1) The expanded cultivation of microbial compound bacterial agent comprises the following steps:

[0046] a. The swill sewage is passed into an 8L aeration tank, and 70g of the microbial composite bacterial agent of formula 4 is dropped into the aeration tank;

[0047] b. After aeration at 35-42°C for 48-72 hours, add 70-98.5v% of the sewage in the aeration tank to the aerobic tank;

[0048] c. Pass the sewage into the aeration tank again, and repeat steps b and c in a cycle;

[0049] 2) The sewage is continuously fed into the aerobic tank and mixed with the sewage from the aeration tank. The residence time of the sewage in the aerobic tank is controlled to 20 hours. The density is 12-15cm / one, and the aeration volume of the aerobic tank is controlled within the range of 100 liters to 500 liters / hour; compared with no film, the naked eye can observe a significan...

Embodiment 3

[0069] The processing of embodiment 3 feces

[0070] 1. Experimental materials:

[0071] 1.1, dried chicken manure 4Kg

[0072] 1.2, 200g of compound bacterial agent of formula 4

[0073] 2. Experiment

[0074] 2.1. At 9:00 on the first morning, put 4Kg of sun-dried chicken manure into a reaction vessel with stirring, put in 200g of compound bacterial agent, let in air, control the flow rate at 1 liter per hour, heat and keep warm under stirring, and the temperature Control at 40-50°C. The entire reaction vessel weighed 16.56 Kg.

[0075] 2.2. At 9:20 in the morning of the next day, the weight was 13.10Kg, and the weight loss was 3.46Kg. This data shows that the composite bacterial agent of the present invention has a very good ability to degrade feces.

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Abstract

The invention relates to a composite microbial inoculum for harmless processing, and its application. The composite microbial inoculum for harmless processing comprises Paracoccus denitrificans, Bacillus megaterium, Bacillus subtilis, Bacillus badius, Bacillus thuringiensis, Bacillus licheniformis, Acinetobacter calcoaceticus, Bacillus laterosporus and Bacillus cereus. The composite microbial inoculum can be used for processing sewage, domestic organic wastes, sewage plant sludge, or human and animal excreta.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial compound bacterial agent for harmless treatment and its application. Background technique [0002] At present, domestic organic waste, sludge, and human and animal manure are mainly disposed of by landfill or dumped into the sea. A few of them are disposed of by incineration, which also brings serious damage to the environment due to the generation of dioxins in the incineration. Secondary pollution. Some are treated with microbial methods, but the effect is not obvious. Contents of the invention [0003] The purpose of the present invention is to provide a kind of microbial composite bacterial agent for the harmless treatment of sewage, domestic organic waste, sewage plant sludge, human and animal feces and its application. [0004] The invention firstly discloses a microbial compound bacterial agent for harmless treatment, including: Paracoccu...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34C02F3/02C02F11/02B09B3/00C12R1/01C12R1/11C12R1/125C12R1/07C12R1/10C12R1/085
CPCY02W10/10Y02W10/20
Inventor 郭景新孙栋林胡业功陆军
Owner WUXI ZHONGQUAN BIOLOGICAL TECH
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