Compound biological enzyme preparation, compound microbial agent and application thereof in treatment of oily sludge

A composite microbial inoculum and composite biological enzyme technology, applied in biological water/sewage treatment, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the complex process, difficult to remove other harmful components of oily sludge, It takes a long time to deal with problems, and achieves the effect of broad application prospects, low cost, and no secondary pollution.

Active Publication Date: 2016-09-28
陕西文岭微生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can significantly reduce the crude oil content of sludge, it has the disadvantages of complex process and difficulty in removing

Method used

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  • Compound biological enzyme preparation, compound microbial agent and application thereof in treatment of oily sludge
  • Compound biological enzyme preparation, compound microbial agent and application thereof in treatment of oily sludge
  • Compound biological enzyme preparation, compound microbial agent and application thereof in treatment of oily sludge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] The present invention will be further described by means of examples below, but the present invention is not limited to such examples.

Embodiment 1

[0049] 1. Purpose of the test:

[0050] Study the separation effect of compound biological enzyme preparation on crude oil and mud in oily sludge, and use compound microbial agent to further treat oil sludge to achieve crude oil recovery and oil sludge harmless treatment.

[0051] 2. Test material:

[0052] 2.1 Oily sludge: CNOOC sludge.

[0053] 2.2 Preparation of compound biological enzyme preparation

[0054] Bacillus subtilis, Bacillus pumilus, Candida Albicans, Lactobacillus breris and their medium were purchased from Xi'an Zirui Biotechnology Co., Ltd. Among them, beef extract peptone medium is used to cultivate Bacillus subtilis and Bacillus pumilus, potato medium is used to cultivate Candida, and MRS medium is used to cultivate Lactobacillus brevis. The composition of the culture medium is as follows:

[0055] Beef extract peptone medium (g / L): beef extract 3, peptone 10, NaCl 5 (1.5% agar should be added to the solid medium);

[0056] Potato medium (g / L): potato ...

Embodiment 2

[0079] 1. Purpose of the test

[0080] PetroChina Safety and Environmental Protection Research Institute and Huabei Oilfield jointly verified the feasibility of promoting the construction process of oily sludge and the effect of harmless treatment of oily sludge by the comprehensive treatment technology of oily sludge compound biological enzyme and microbial method.

[0081] 2. Test materials

[0082] 2.1 Huabei oilfield oil sludge;

[0083] 2.2 Preparation of compound biological enzyme preparation

[0084] The composite biological enzyme preparation prepared in Example 1 was adopted.

[0085] 2.3 Preparation of composite microbial agent.

[0086] The composite microbial inoculum prepared in Example 1 was adopted.

[0087] 3. Test steps

[0088] In this experiment, the amount of oily sludge treated in each batch is 5t (ton).

[0089] (1) The test is carried out in two steps:

[0090] a. Use compound biological enzyme preparation to separate and elute crude oil, asphalt,...

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Abstract

The invention provides a compound biological enzyme preparation, a compound microbial agent and application thereof in the treatment of oily sludge. The compound biological enzyme preparation is prepared from bacillus subtilis, bacillus pumilus, candida and lactobacillus brevis. The compound microbial agent is prepared from candida tropicalis, sphingobacterium multivolume, bacillus cereus, acinetobacter calcoaceticus and bacillus megaterium. The invention also provides the application of the compound biological enzyme preparation and / or the compound microbial agent in the treatment of oily sludge and a method for treating the oily sludge. The method comprises steps of treating the oily sludge with the compound biological enzyme preparation to form an upper oil phase, an intermediate aqueous phase and a lower sludge phase, and treating the lower sludge phase and / or the intermediate aqueous phase by using the composite microbial agent. The compound biological enzyme preparation, the compound microbial agent and the application thereof provided by the invention have the advantages of harmless treatment of oily sludge, low cost, simplicity and convenience, no secondary pollution, and broad application prospects.

Description

Technical field [0001] The invention belongs to the application field of oil field environmental protection technology, and relates to the treatment technology of oily sludge, more specifically, the invention relates to the biological treatment technology of oily sludge, especially the composite biological enzyme preparation and composite microorganism for treating oily sludge Bacteria and its application in oily sludge. Background technique [0002] Oily sludge is a kind of suspension emulsion system with extremely complex composition, which mainly comes from oil and gas exploitation, oil field gathering and transportation, oil refinery and oily sewage treatment process. Improper disposal will pose a huge threat to the environment. At present, domestic and foreign treatment methods for oily sludge generally include: incineration, hot washing, solvent extraction, chemical demulsification, solid-liquid separation, coking, reuse, profile control, etc. Although these methods ca...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C12N1/16C02F101/32C02F103/10C12R1/125C12R1/07C12R1/72C12R1/24C12R1/74C12R1/085C12R1/11C12R1/01
CPCC02F3/34C02F3/342C02F3/347C02F2101/32C02F2103/10C12N1/16C12N1/20
Inventor 陈五岭杜茂林
Owner 陕西文岭微生物科技有限公司
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