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Heterotrophic nitrification-aerobic denitrification composite microbial inoculum with salt tolerance and high ammonia nitrogen tolerance, preparation and application thereof

An aerobic denitrification and compound bacterial agent technology, applied in the field of microorganisms, can solve the problems of microbial cell rupture, microbial poisoning, poisoning, etc., and achieve the effects of effectively removing high-concentration ammonia nitrogen and total nitrogen, strong tolerance, and convenient use.

Active Publication Date: 2020-04-10
CHONGQING UNIV OF TECH
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  • Description
  • Claims
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Problems solved by technology

However, the traditional biological denitrification method cannot directly treat high-ammonia nitrogen wastewater, and often needs to be combined with physical and chemical methods, mainly because the free ammonia (NH3) released by high-concentration ammonia nitrogen can quickly diffuse into the cell through the cell membrane. Poison the nitrifying bacteria in the traditional biological method to inhibit the biochemical reaction
At the same time, the toxic effect of high-concentration salt (calculated as NaCl, total salt content > 1%) on microorganisms is also very obvious. High salt can change the osmotic pressure of microbial cells, causing microbial cell rupture or inhibiting growth, thereby affecting the removal of organic matter. rate, ammonia nitrogen removal rate
Therefore, how to effectively remove high-concentration ammonia nitrogen under high-salt conditions has become a huge challenge for traditional biological methods.

Method used

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  • Heterotrophic nitrification-aerobic denitrification composite microbial inoculum with salt tolerance and high ammonia nitrogen tolerance, preparation and application thereof
  • Heterotrophic nitrification-aerobic denitrification composite microbial inoculum with salt tolerance and high ammonia nitrogen tolerance, preparation and application thereof
  • Heterotrophic nitrification-aerobic denitrification composite microbial inoculum with salt tolerance and high ammonia nitrogen tolerance, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Preparation of Heterotrophic Nitrification-Aerobic Denitrification Compound Bacteria

[0047] Step 1) acclimatization of bacterial classification

[0048] Calcium acetate-acinetobacter Acinetobacter calcoaceticus TNJ-1 high salt, high ammonia nitrogen tolerance domestication:

[0049] Inoculate 2 mL of bacterial liquid into 100 mL of sterilized heterotrophic nitrification medium with a salinity of 5%, shake well, and culture at 30°C and 150 r·min-1 for 2 days. Continuous subculture under the same conditions above, a total of 5 times, each subculture is domesticated by continuously increasing the concentration of ammonia nitrogen in the heterotrophic nitrification medium, the concentration of ammonia nitrogen in different subculture medium is 200mg / L, 300mg / L, 400mg respectively / L, 500mg / L, 600mg / L.

[0050] Pseudomonas stutzeri (CICC 10402) high salt, high ammonia nitrogen tolerance domestication:

[0051] Inoculate 2 mL of bacterial liquid into 100 mL of...

Embodiment 2

[0068] Example 2: Experiment of removal of high ammonia nitrogen by compound bacterial agent under the condition of salinity 5%

[0069] First, add K2HPO4 5.0g, MgSO4 7H2O 2.5g, FeSO4 7H2O 0.05g, MnSO4 4H2O 0.05g, CuSO4 0.2g, CdSO4 0.05g into 1000mL deionized water respectively, and configure it as a trace element solution as a spare. Then add (NH4)2SO4 2g, C6H12O6 10.64g, NaCl 5g respectively in 1000mL deionized water, above-mentioned trace element solution 50mL, be configured into the heterotrophic nitrification medium that the initial concentration of ammonia nitrogen is 400mg / L, adjust the heterotrophic nitrification medium pH to 7.0.

[0070] Take 100mL of the above-mentioned abnormal nitrification culture based on a 250mL Erlenmeyer flask, add 2mL of compound bacterial agent bacterial solution (OD600 is 1-2) into the bottle with a micropipette, in which calcium acetate-Acinetobacter TNJ-1, The contents of Pseudomonas stutzeri, Halomonas denitrifier, and Copper greedy ba...

Embodiment 3

[0071] Example 3: The experiment of removing high ammonia nitrogen by compound bacterial agent under different salinity conditions

[0072] First, add K2HPO4 5.0g, MgSO4 7H2O 2.5g, FeSO4 7H2O 0.05g, MnSO4 4H2O 0.05g, CuSO4 0.2g, CdSO4 0.05g into 1000mL deionized water respectively, and configure it as a trace element solution as a spare. Then add (NH4)2SO4 2g, C6H12O6 10.64g, NaCl 1g respectively in 1000mL deionized water, and 50mL of the above trace element solution, and configure it as a heterotrophic nitrification medium with an ammonia nitrogen concentration of 400 mg / L and a salinity of 1%. According to the above-mentioned method, by changing the NaCl weight that adds is 3g, 5g, 7g, 9g, configuration ammonia nitrogen concentration is 400mg / L, salinity is respectively the heterotrophic nitrification medium of 3%, 5%, 7%, 9%, and Adjust the pH of the heterotrophic nitrification medium to 7.0.

[0073] Take 100mL of the above-mentioned heterotrophic nitrification cultures w...

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Abstract

The invention relates to heterotrophic nitrification-aerobic denitrification composite microbial inoculum with salt tolerance and high ammonia nitrogen tolerance, preparation and application thereof.The composite microbial inoculum is prepared by compounding of Acinetobacter calcoaceticus TNJ-1, pseudomonas stutzeri, Halomonas denitrificans and Cupriavidus sp. SWA1. Through the synergistic actionof the four microorganisms, the effective treatment of high ammonia nitrogen wastewater can be realized under aerobic and high-salt conditions, and toxic substances such as chlorides, heavy metals and the like can be effectively resisted.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a heterotrophic nitrification-aerobic denitrification composite bacterial agent capable of resisting salt and high ammonia nitrogen and its preparation and application. Background technique [0002] In recent years, high ammonia nitrogen wastewater (ammonia nitrogen concentration > 200mg / L) has been increasing. Due to improper treatment and direct discharge into water bodies, eutrophication and other phenomena have become increasingly serious, resulting in deterioration of water quality, which in turn pollutes soil and leads to crop yield reduction and agricultural product quality. affect the quality of the air environment. Biological nitrogen removal is considered to be the most effective water nitrogen removal method due to its advantages of no secondary pollution, economy and safety. However, the traditional biological denitrification method cannot directly tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/01C12R1/38C02F101/20C02F101/16C02F101/12
CPCC12N1/20C12N1/36C02F3/34C02F2101/16C02F2101/22C02F2101/206C02F2101/20C02F2101/12Y02W10/10
Inventor 赵天涛刘毫艾铄张千封丽滕亮余欢鲁登富
Owner CHONGQING UNIV OF TECH
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