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Agricultural compound microbial agent and application thereof

A composite microbial inoculum and agricultural technology, applied in the field of agricultural composite microbial inoculants, can solve problems such as ineffectiveness, poor synergy, and unsatisfactory effects, and achieve the effects of promoting crop growth, developing root systems, and improving the quality of agricultural products.

Active Publication Date: 2020-06-05
福建省致青生态环保有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the single microbial agent has a single effect and the effect is not ideal; some compound microbial agents have poor stability due to the poor synergistic ability of the selected strains, and cannot achieve the corresponding effect.

Method used

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  • Agricultural compound microbial agent and application thereof
  • Agricultural compound microbial agent and application thereof
  • Agricultural compound microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0029] In the present invention, the preparation method of the solid bacterial powder preferably includes the following steps: after activating the strain, carry out continuous two-stage seed liquid culture, insert into the fermentation medium for fermentation, and concentrate to obtain concentrated bacterial liquid; The concentrated bacterial solution of Enterobacter aerogenes CT-B09-2 and Acinetobacter calcoaceticus WYS-A01-1 was mixed with a protective agent (skimmed milk powder) and then freeze-dried to obtain the solid bacterial powder; the Bacillus amyloliquefaciens The fermentation broth of JL-B05, Bacillus amyloliquefaciens JL-B06 and Bacillus licheniformis LYN-3 is mixed with light calcium carbonate and then spray-dried to obtain the solid bacteria powder.

[0030] In the present invention, the strains are inoculated in NB medium and cultured for 15-25 hours to obtain a primary seed solution; the culture temperature is preferably 25-35°C, and the culture speed is prefera...

Embodiment 1

[0036] (1) Preparation of Enterobacter aerogenes CT-B09-2 bacterial liquid and solid bacterial powder:

[0037] Preparation of Enterobacter aerogenes CT-B09-2 primary seed solution:

[0038] Activate Enterobacter aerogenes CT-B09-2 strain, pick a single colony, inoculate in 150ml NB medium (sterilize for 30min at 121°C), 28°C, 180 r min -1 Under the condition of shaking culture for 20 h, the primary seed solution was obtained.

[0039] Enterobacter aerogenes CT-B09-2 secondary seed liquid preparation:

[0040] Put the first-grade seed solution into a sterile seed tank, the inoculum amount is 0.5% ~ 1.0% (V / V); the culture conditions are: manually adjust the speed and air flow of ventilation to adjust DO (DO40%), and the culture temperature is 25℃~ 35℃, the speed is 200~350r·min -1 , tank pressure 0.05MPa; cultivation time 10~15 h. OD of secondary seed solution 600 The value is between 0.6 and 0.8, at this time the strain is the strongest.

[0041] Preparation of Enterob...

Embodiment 2

[0084] Application of solid preparation of agricultural composite microbial agent in tomato planting

[0085] A plot of 3 mu of tomato was selected for 5 consecutive years, and the soil for testing was fluvo-aquic soil with medium loam texture, flat terrain, good irrigation and drainage conditions, and the soil conditions were representative. The planted tomato variety is Zhongshu No. 5, planted in double rows, with a large row spacing of 70 cm, a small row spacing of 45 cm, and a plant spacing of 35 cm. 3500 plants were transplanted per mu.

[0086] Test method: 8 treatments were set up, and each treatment had 3 repetitions. The plots are randomly arranged, and the plot area is 6×10=60 square meters. Treatment 1: conventional fertilization + flushing the agricultural compound microbial bacterial agent solid preparation 1 prepared in Example 1, 1 kg / mu; treatment 2: conventional fertilization + Enterobacter aerogenes CT-B09-2 solid bacterial powder, 1 kg / mu ; Treatment 3: C...

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Abstract

The invention discloses an agricultural compound microbial agent and application thereof, belonging to the technical field of microorganisms. The agricultural compound microbial agent comprises enterobacter aerogenes CT-B09-2, acinetobacter calcoaceticus WYS-A01-1, bacillus amyloliquefaciens JL-B05, bacillus amyloliquefaciens JL-B06 and bacillus licheniformis LYN-3, wherein the total viable bacteria concentration is (5-8)*10<8> cfu / ml or (2-3)*10<9> cfu / g. The agricultural compound microbial agent can promote the growth of crops, improves the disease-preventing, disease-resisting, stress-resisting and continuous-cropping-resisting capabilities of the crops, increases the yield of the crops and improves the quality of agricultural products.

Description

technical field [0001] The invention belongs to the technical field of microbial application, and in particular relates to an agricultural compound microbial bacterial agent and its application. Background technique [0002] At present, soils all over the world are facing problems such as deterioration of soil properties, soil acidification, salinization, and destruction of soil microbial diversity, which directly lead to soil barrenness and soil failure, which affect the sustainable development of agriculture. Under the situation of such poor soil ecology, how to improve soil, enhance soil fertility, improve soil ecological environment, and improve crop yield and quality has become the top priority of current agricultural work. [0003] Microorganisms can promote the release and chelation of trace elements in the soil through the exchange of oxygen and carbon dioxide and the secretion of organic acids and other acidic substances during the metabolic process, effectively bre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/01C12R1/07C12R1/10
CPCC05F5/002C05G3/00C12N1/20
Inventor 袁宗胜刘芳张国防黄秋良
Owner 福建省致青生态环保有限公司
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