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Industrial preparing process, fermented liquid and use of spheroidal erythrocyte

A technology of Rhodobacter sphaeroides and fermentation liquid, which is applied in chemical instruments and methods, botany equipment and methods, bacteria, etc., and can solve the problem that Rhodobacter sphaeroides is limited to the laboratory

Inactive Publication Date: 2007-06-27
无锡中科活力生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the research on Rhodobacter sphaeroides is limited to the laboratory level, and there is no technical scheme for using Rhodobacter sphaeroides to carry out industrial-scale submerged fermentation methods
At the same time, in the beverage industry, there is a lack of a beverage that can greatly promote human health by using bio-fermentation technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0148] Preparation method of fermentation broth:

[0149] The proportion of inorganic salt solution in 1000ml distilled water;

[0150] MgSO 4 ·7H 2 O 0.3 g

[0151] CaCl·2H 2 O 0.05 g

[0152] FeSO 4 ·7H 2 O 0.5 g

[0153] NaEDTA 0.5 g

[0154] The proportion of trace element solution in 250ml distilled water;

[0155] H 3 BO 4 1.0g

[0156] MnSO 4 120 mg

[0157] NaMoO 4 60 mg

[0158] ZnSO 4 15 mg

[0159] Cu(NO 3 ) 2 2 mg

[0160] Sodium molybdate 12 mg

[0161] Sodium selenite 12 mg;

[0162] Proportion of growth factor solution in 100ml distilled water

[0163] Biotin 0.5 mg

[0164] Vitamin B 1 10 mg

[0165] Niacin 10 mg

[0166] P-Aminobenzoic acid 10 mg;

[0167] Lactic acid solution ratio

[0168] 900ml distilled water

[0169] 40 ml of lactic acid, adjust the pH to 6.4 with 12N NaOH solution;

[0170] Ammonium sulfate solution ratio

[0171] 800ml distilled water

[0172] Ammonium sulfate 5 g

[0173] Add water to 1000 ml;

[0174] After diss...

Embodiment 2

[0188] The proportion of inorganic salt solution in 1000ml distilled water;

[0189] MgSO 4 ·7H 2 O 3.0 g

[0190] CaCl·2H 2 O 1.0 g

[0191] FeSO 4 ·7H 2 O 1.0 g

[0192] NaEDTA 1.0g

[0193] The proportion of trace element solution in 250ml distilled water;

[0194] H 3 BO 4 1.5g

[0195] MnSO 4 200 mg

[0196] NaMoO 4 100 mg

[0197] ZnSO 4 20 mg

[0198] Cu(NO 3 ) 2 3 mg

[0199] Sodium molybdate 15 mg

[0200] Sodium selenite 15 mg;

[0201] Proportion of growth factor solution in 100ml distilled water

[0202] Biotin 0.5 mg

[0203] Vitamin B 1 30 mg

[0204] Niacin 30 mg

[0205] P-Aminobenzoic acid 20 mg;

[0206] Lactic acid solution ratio

[0207] 900ml distilled water

[0208] 100 ml of lactic acid, adjust the pH to 6.4 with 12N NaOH solution;

[0209] Ammonium sulfate solution ratio

[0210] 800ml distilled water

[0211] Ammonium sulfate 8 g

[0212] Add water to 1000 ml;

[0213] After dissolving, add distilled water to 1000ml, then adjust ...

Embodiment 3

[0227] The proportion of inorganic salt solution in 1000ml distilled water;

[0228] MgSO 4 ·7H 2 O 0.2 g

[0229] CaCl·2H 2 O 0.1 g

[0230] FeSO 4 ·7H 2 O 0.1 g

[0231] NaEDTA 0.1 g

[0232] The proportion of trace element solution in 250ml distilled water;

[0233] H 3 BO 4 0.5g

[0234] MnSO 4 50 mg

[0235] NaMoO 4 20 mg

[0236] ZnSO 4 10 mg

[0237] Cu(NO 3 ) 2 2 mg

[0238] Sodium molybdate 6 mg

[0239] Sodium selenite 6 mg;

[0240] Proportion of growth factor solution in 100ml distilled water

[0241] Biotin 0.2 mg

[0242] Vitamin B 1 10 mg

[0243] Niacin 10 mg

[0244] P-Aminobenzoic acid 10 mg;

[0245] Lactic acid solution ratio

[0246] 900ml distilled water

[0247] 30 ml of lactic acid, adjust the pH to 6.4 with 12N NaOH solution;

[0248] Ammonium sulfate solution ratio

[0249] 800ml distilled water

[0250] Ammonium sulfate 3 g

[0251] Add water to 1000 ml;

[0252] After dissolving, add distilled water to 1000ml, then ad...

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PUM

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Abstract

The present invention discloses production process of multifunctional fermented liquid with bacterium, and is especially functional fermented liquid producing process with Rhodobacter sphaeroides. The Rhodobacter sphaeroides has preservation number of CGMCC No.1562. The production process includes separation from culture dish, strengthening of the bacterial seed in a shaking bottle, expanded culturing in the first seed tank (200L) and secondary seed tank (2000L) and large scale fermentation in large fermenting tank of 5000-20000 L.

Description

Technical field [0001] The invention relates to a production method of using bacterial fermentation to make a functional fermentation broth, in particular to a production method of using Rhodobacter sphaeroides to make a functional fermentation broth. Background technique [0002] At present, the research on Rhodobacter sphaeroides is limited to the laboratory level, and the technical solution of Rhodobacter sphaeroides is not used for industrial-scale deep fermentation methods. At the same time, in the beverage industry, there is a lack of a beverage that uses biological fermentation technology to produce a beverage that can greatly promote human health. Summary of the invention [0003] The purpose of the present invention is to provide a deep-level industrial-scale fermentation method using Rhodobacter sphaeroides, the resulting fermentation broth is beneficial to human health, can regulate the human immune system, and increase human resistance . [0004] In the inventor's se...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/23A01N63/02C02F3/34A23K1/16A62D3/00A23K10/18A23L27/24
Inventor 王栋
Owner 无锡中科活力生物技术有限公司
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