Production method of agricultural photosynthetic bacteria preparation

A photosynthetic bacteria preparation and production method technology, applied in the field of microorganisms, can solve problems such as unstable product quality and application effect, unstable nutritional content of raw materials, and difficult promotion and use, so as to ensure sustainable development, restore soil fertility, and ensure safety Effect

Active Publication Date: 2009-12-09
SHUNDE HONGLONG BIOLOGY SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This plant growth regulator is also a liquid preparation produced by photosynthetic bacteria, but because it uses animal manure and waste water as fermentation raw materials, the nutrient content of the raw materials is unstable, and it is produc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In 1.0 liter of distilled water, add 1.0 grams of sodium malate; 1.0 grams of sodium acetate; 1.0 grams of ammonium chloride; 1.0 grams of potassium dihydrogen phosphate; 0.1 grams of calcium chloride; 3.0 grams of sodium bicarbonate; 0.5 grams of magnesium chloride; 1.0ml; vitamin solution 1.0ml; yeast extract 0.5g. Adjust the pH to 6 with sodium hydroxide, put it into a glass bottle, and sterilize it at 121°C for 30 minutes. When it is cooled to 30°C, add 5% of the mixed culture of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaericus. The mixing ratio is 1:1. Under the condition of temperature 15°C and light intensity of 800lux, anaerobic culture was carried out for 3 days, and the bacterial content reached 5.2X10 8 cfu / ml, it becomes a reddish-brown strain culture solution.

[0036]Then appropriate amount of various components of the fermentation production medium was dissolved in water in proportion and added to the fermenter: 2.7 gra...

Embodiment 2

[0040] In 1.0 liter of distilled water, add 1.0 grams of sodium malate; 1.0 grams of sodium acetate; 1.0 grams of ammonium chloride; 1.0 grams of potassium dihydrogen phosphate; 0.1 grams of calcium chloride; 3.0 grams of sodium bicarbonate; 0.5 grams of magnesium chloride; 1.0ml; vitamin solution 1.0ml; yeast extract 0.5g. Adjust the pH to 8 with sodium hydroxide, put it into a glass bottle, and sterilize it at 121°C for 30 minutes. When the medium is cooled to 35°C, add 40% of the mixed culture of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaericus , the mixing ratio is 1:1. Under the condition of temperature 35℃ and light intensity 3500lux, anaerobic culture for 4 days, the bacteria content reached 6X10 8 cfu / ml, it becomes a red culture medium.

[0041] Then appropriate amount of various components of the fermentation production medium was dissolved in water in proportion and added to the fermenter: 2.7 grams of sodium acetate; 1.3 grams of...

Embodiment 3

[0044] In 1.0 liter of distilled water, add 1.0 grams of sodium malate; 1.0 grams of sodium acetate; 1.0 grams of ammonium chloride; 1.0 grams of potassium dihydrogen phosphate; 0.1 grams of calcium chloride; 3.0 grams of sodium bicarbonate; 0.5 grams of magnesium chloride; 1.0ml; vitamin solution 1.0ml; yeast extract 0.5g. Use sodium hydroxide to adjust the pH to 8, put it into a glass bottle, and sterilize it at 121°C for 30 minutes. When the medium is cooled to 32°C, add 20% of the mixed culture of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaericus , the mixing ratio is 1:1. Under the condition of temperature 20℃ and light intensity 2000lux, anaerobic culture for 4 days, the bacteria content reached 5X10 8 cfu / ml, it becomes a red culture medium.

[0045] Then appropriate amount of various components of the fermentation production medium was dissolved in water in proportion and added to the fermenter: 2.7 grams of sodium acetate; 1.3 grams ...

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Abstract

The invention relates to a production method of an agricultural photosynthetic bacteria preparation, adopting the following steps: 1. strain amplification culture: adjusting the pH value of a strain amplification medium from 6.0 to 8.5; placing the medium into a glass bottle and carrying out heat disinfection; inoculating a mixed culture of two photosynthetic bacteria including Rhodopeudomonas palustris and Rhodobacter sphaeroides based on 5-40% of the volume of a strain medium; anaerobically culturing the mixed culture for 3-4 days under the conditions that the temperature is 15-35 DEG C and the illumination intensity is 1000-3000lux, thus obtaining strain culture fluid; 2. fermentation production: placing a fermentation medium into a fermentation cylinder and carrying out heat disinfection; inoculating the strain culture fluid based on the inoculation amount, namely 5-40% of the volume of the fermentation production medium; the strain culture fluid is fermented for 6 days to obtain the agricultural photosynthetic bacteria preparation under the conditions that the temperature is 28-30 DEG C, the stirring rate is 150-300r/m, the cylinder pressure is kept between 0.02MPa and 0.05MPa and the illumination intensity is 2000-3000lux. Compared with the prior art, the preparation has the advantages of stable product quality and diversified functions.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a production method of an agricultural photosynthetic bacteria preparation. Background technique [0002] Photosynthetic bacteria are a group of bacteria that can use light energy as an energy source. Because it can not only promote the transformation of soil substances, improve soil fertility, but also promote crop growth, enhance crop resistance and disease prevention, degrade pesticide residues and fix toxic and harmful heavy metals, so crops can receive the effect of increasing yield and quality by applying its preparation . However, in order to promote the use of crops on a large scale, the quality of the applied photosynthetic bacteria preparation must be stable and the effect must be good. Chinese Invention Patent No. 94106358.5 discloses a technology called "plant growth regulator produced by microorganisms and its production method". This plant growth regulator is also a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00A01P3/00A62D3/02B09C1/10C12R1/01A62D101/04A62D101/47A62D101/26A62D101/22
Inventor 张肇铭黄振江吕俭雄李琦何锦霞潘广驹罗根周超南
Owner SHUNDE HONGLONG BIOLOGY SCI & TECH
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