In situ cultivation device, and method of high through-put separating and screening microorganism

A technology of in situ culture and microorganisms, applied in the directions of separating microorganisms, supporting/immobilizing microorganisms, and sampling methods of biological materials, etc., can solve problems such as hindering the development of microbial resources and research on the law of microbial life activities, and achieve the effect of high-efficiency separation

Active Publication Date: 2019-11-08
PEKING UNIV
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  • Abstract
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Problems solved by technology

However, due to the limitations of current technical means, only a very small number of microorganisms in nature can be isolated and cultivated, which seriously hinders the research on the laws of microbial life activities and the development of microbial resources.

Method used

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  • In situ cultivation device, and method of high through-put separating and screening microorganism
  • In situ cultivation device, and method of high through-put separating and screening microorganism
  • In situ cultivation device, and method of high through-put separating and screening microorganism

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Embodiment Construction

[0043] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments. However, it should be understood that the accompanying drawings are provided only for better understanding of the present invention, and they should not be construed as limiting the present invention.

[0044] Such as Figure 1-6 , Figure 11 , Figure 12 As shown, the present invention provides a kind of in-situ culture device, and it comprises in-situ bacteria separation device, and in-situ bacteria separation device comprises:

[0045] A vacuum chassis 1, the upper part of which is formed with a vacuum groove 11, and the lower part is provided with a cylindrical ring 12 communicating with the vacuum groove 11;

[0046] The clamp lower layer 2 is fastened to the top of the vacuum chassis 1, and the bottom plate 21 of the clamp lower layer 2 is formed with a through hole 22 communicating with the vacuum groove 11;

[0047] The chip 3 is placed on t...

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Abstract

The invention discloses an in situ cultivation device, and a method of high through-put separating and screening microorganism. The in situ cultivation device comprises an in situ bacterium separatingdevice, the in situ bacterium separating device comprises a vacuum chassis, a clamping lower layer, a chip, a clamping pressing sheet and a clamping upper layer, the upper part of the vacuum chassisis provided with a vacuum groove, and lower part of the vacuum chassis is provided with a circular cylinder ring communicating with the vacuum groove, the clamping lower layer is connected with the top of the vacuum chassis in a fastening mode, and a base plate of the clamping lower layer is provided with through holes communicating with the vacuum groove, the chip is placed on the base plate of the clamping lower layer, the chip is evenly provided with a plurality of cultivation through holes, the clamping pressing sheet is arranged on the chip in a pressing mode, the clamping pressing sheetis provided with a plurality of sample-adding holes one-one corresponding to the cultivation through holes on the chip, the clamping upper layer is fixedly arranged above the clamping lower layer, andthe clamping upper layer is provided with a sample-adding window.

Description

technical field [0001] The invention relates to an in-situ culture device and a method for high-throughput separation and screening of microorganisms, in particular to a device for high-throughput in-situ separation of functional microorganisms. Background technique [0002] Microorganisms are ubiquitous in nature and play an important role in the environment we live in. However, due to the limitations of current technical means, only a very small number of microorganisms in nature can be isolated and cultivated, which seriously hinders the research on the laws of microbial life activities and the development of microbial resources. Contents of the invention [0003] In view of the above problems, the object of the present invention is to provide an in situ culture device and a method for high-throughput separation and screening of microorganisms, which can quickly and efficiently separate microorganisms in the environment, and can be used for basic and applied research on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/12C12M1/26C12N1/00C12N1/02
CPCC12M23/02C12M25/02C12M33/12C12N1/00C12N1/02
Inventor 王淼啸聂勇王健伟吴晓磊
Owner PEKING UNIV
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