Antimicrobial composition

A composition and plant technology, applied in the direction of antibacterial drugs, drug combinations, applications, etc., can solve the problems of ineffective sterilization, Gram-negative bacteria do not have antibacterial activity, etc., and achieve excellent safety effects

Inactive Publication Date: 2015-09-16
ECO FRIENDLY INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nisin alone does not have antibacterial activity against gram-negative bacteria, so it has no effect on eradicating periodontal disease pathogens such as Porphyromonas gingivalis, which is a gram-negative bacterium

Method used

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  • Antimicrobial composition
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Antibacterial activity against Porphyromonas gingivalis (1)

[0050] The periodontal disease pathogen Porphyromonas gingivalis ATCC33277 strain was cultured in Enriched Tryptic Soy Broth (e-TSB) to logarithmic growth phase. Dilute the culture solution with the citric acid buffer (pH4.5) that has added 0.8%NaCl, adjust the number of bacteria (10 6 CFU / mL). The test solution was diluted with sterilized purified water, and adjusted to 5 times concentrated plum extract (3mg / mL, 15mg / mL converted before concentration), nisin (150μg / mL), nisin (150μg / mL) + 5 times concentrated plum Extract (3mg / mL, 15mg / mL before concentration). This diluted culture solution (100 μL) was added to the test solution (5 mL). After contacting at room temperature for 1 minute, the liquid (100 μL) was added and mixed to e-TSB medium (5 mL), and the mixed solution (100 μL) was immediately plated on an anaerobic blood agar medium for After 5 days of anaerobic cultivation, the number of...

Embodiment 2

[0054] Example 2: Antibacterial activity against Porphyromonas gingivalis (2)

[0055]Porphyromonas gingivalis strain W83, the pathogenic bacteria of periodontal disease, was added to the brain heart infusion broth (Brain heart infusion) added with hemin (5 mg / L) and menadione (1 mg / L) culture medium to logarithmic growth phase. The culture solution was diluted with citric acid buffer added with 0.8% NaCl to adjust the number of bacteria (10 6 CFU / mL). The test solution was prepared with sterilized purified water to prepare 5 times concentrated plum extract (10mg / mL, calculated as 50mg / mL before concentration), nisin (20μg / mL), 5 times concentrated plum extract (10mg / mL, calculated as concentrated 50mg / mL) + nisin (20μg / mL), use a 96-well plate for 2-fold serial dilution. Add brain heart infusion broth medium (140 μL) and bacterial solution (10 μL) supplemented with hemin (5 mg / L) and menadione (1 mg / L) to each well (50 μL), and incubate for 48 hours Afterwards, the turbid...

Embodiment 3

[0057] Embodiment 3: antibacterial activity (1) to escherichia coli

[0058] Gram-negative bacteria, Escherichia coli (Escherichia coli, Escherichia coli) NBRC3301 strain, were cultured in Tryptic Soy Broth (TSB) until the logarithmic growth phase. Dilute the culture solution with sterilized purified water to adjust the number of bacteria (10 3 ~10 4 CFU / mL). The test solution was diluted with sterilized purified water and adjusted to nisin (10μg / mL), 5 times concentrated plum extract (3mg / mL, 15mg / mL based on conversion before concentration), nisin (10μg / mL) + 5 times concentrated plum extract Extract (3mg / mL, 15mg / mL before concentration). This diluted culture solution (10 μL) was added to the test solution (1 mL). After a certain number of days (0 to 7 days) had elapsed after contacting at room temperature, the liquid (100 μL) was plated on a TSB agar medium, and after culturing for 48 hours, the number of colonies formed on the plate was measured. The results are show...

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Abstract

The present invention provides a highly safe antibacterial composition showing antibacterial activity against both Gram-positive bacteria and Gram-negative bacteria, of which flavor is not impaired. The antibacterial composition of the present invention contains nisin, and one or two or more kinds of plant-derived ingredients selected from the group consisting of juice, extract, and distillate of a Rosaceae plant, and a mixture of these. It is preferred that the Rosaceae plant is a plant belonging to the genus Prunus , it is more preferred that the plant belonging to the genus Prunus is Prunus mume , and it is particularly preferred that the plant-derived ingredient is Prunus mume fruit juice. The antibacterial composition of the present invention is useful especially as a composition for oral cavity.

Description

technical field [0001] The present invention relates to an antibacterial composition which contains nisin as a bactericidal component and is effective against both Gram-positive bacteria and Gram-negative bacteria. Background technique [0002] Nisin is a kind of bacteriocin, which is a polycyclic antibacterial peptide composed of 34 amino acid residues produced by Lactococcus lactis isolated from cheese. Nisin is a water-soluble polypeptide, and even at a low concentration of about 1 ppb, it has a growth inhibitory effect against Gram-positive bacteria represented by acetic acid bacteria, and it is different from other bacteriocins that have antibacterial activity only against similar species different, with a broad antibacterial spectrum. Nisin is approved as a food additive in many countries including Japan, and is used in food preservatives or various antibacterial agents. [0003] Since nisin has antimicrobial activity against Gram-positive bacteria, it is expected to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N65/34A61K8/64A61K8/97A61Q11/00
CPCA61K8/97A61K8/64A61Q11/00A01N65/00A61K8/9789A61P31/04A61P43/00A01N43/90A01N65/34A61K9/0063A61K36/736A61K38/164
Inventor 永利浩平
Owner ECO FRIENDLY INST
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