Method and reagent kit for establishing experimental model of diabetic foot ulcer rat with mixed infection of Gram positive bacteria and negative bacteria

Abstract

The invention discloses a method and a reagent kit for establishing an experimental model of a diabetic foot ulcer rat with mixed infection of Gram positive bacteria and Gram negative bacteria. The method comprises the following steps: after the rat is fed by a high fat feed for eight weeks, injecting streptozotocin to generate a diabetic model, after the diabetic model is stable, generating a scald with the depth of II degrees in the skin of the diabetic rat, and three days later, injecting mixed suspension of the Gram positive bacteria and the Gram negative bacteria at the scald. In addition, the invention also discloses the reagent kit for establishing the experimental model of the diabetic foot ulcer rat with the mixed infection of the Gram positive bacteria and the Gram negative bacteria. The reagent kit comprises the mixed suspension of the Gram positive bacteria and the Gram negative bacteria. According to the diabetic ulcer experimental animal model disclosed by the invention, by detecting the changes of biochemical indicators after drug intervention, results show that the model has the characteristic of being capable of generating the diabetic foot ulcer with various bacteria colony infections, and the characteristics of long healing time of an ulcer wound surface, sensitivity to drug reaction and the like.

Description

technical field [0001] The invention belongs to the field of experimental animal models, and in particular relates to a method and a kit for establishing an experimental model of diabetic foot ulcer rats infected with Gram-positive bacteria and negative bacteria. Background technique [0002] With the sharp increase in the incidence of diabetes in my country, the number of patients with diabetic ulcers has also increased. Establishing a disease model that truly simulates diabetic ulcers at the overall animal level is an important tool for medical research such as exploring the pathogenesis of diabetic ulcers and preclinical pharmacodynamic evaluation. It has very important scientific and clinical significance. [0003] At present, animal models of diabetic foot ulcers are mostly induced animal models. The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease,...

AI Technical Summary

Problems solved by technology

The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease, neuropathy or infection), and there is a big gap between the similarity between the animal model and the actual simulation and the research purpose, and the actual The combination of two or more factors is more common in clinical practice

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