Method and reagent kit for establishing experimental model of diabetic foot ulcer rat with mixed infection of Gram positive bacteria and negative bacteria
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A technology for gram-positive bacteria and diabetic foot ulcers, which is applied in the field of establishing experimental models of diabetic foot ulcer rats with mixed infection of gram-positive bacteria and negative bacteria
Inactive Publication Date: 2013-12-25
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease, neuropathy or infection), and there is a big gap between the similarity between the animal model and the actual simulation and the research purpose, and the actual The combination of two or more factors is more common in clinical practice
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Embodiment 1
[0022] Rats with type 2 diabetes mellitus were induced by high-fat feed combined with streptozotocin, skin ulcers were induced by deep second-degree burns using a scald instrument, and quality control bacteria Staphylococcus aureus-ATCC25923 (Staphylococcus aureus) Escherichia coli-ATCC25922 (Escherichia coli, E.coli) strain equal mixture was used to make rat model of diabetic foot ulcer infection.
[0023] 1 Materials and methods
[0024] 1.1 Animals: SD male rats, SPF grade, body weight (180±10) g, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.
[0025] 1.2 Main reagents and drugs: Streptozotocin (STZ), purchased from Alexis Company; high-fat feed (ingredients: basic feed 73%, lard 20%, milk powder 2%, cholesterol 1%, sucrose 4%; The basic feed formula: 15% barley, 31% wheat, 15% corn, 15% bran, 6% fishmeal, 18% soybean meal, oil and salt, the basic feed was purchased from the Naval Medical Research Institute of the Chines...
Embodiment 2
[0038] Example 2 Western medicine-recombinant bovine basic fibroblast growth factor for external use (trade name: Beifuji) intervention test results
[0039] 1. Method: Normal ulcer group (N), diabetic ulcer group (DU), infection control group (IU-C) changed daily normal saline dressing, infection treatment group (IU-T) daily dressing change of Beifuji 800iu wound . Measure 0d, 3d, 10d after dressing change, wound area after healing, 3% pentobarbital sodium in 2ml Kg after healing -1 The dose was anesthetized by intraperitoneal injection, blood was collected from the abdominal aorta, serum was separated by centrifugation, and skin tissue was taken for examination.
[0040] 2. Data processing: SPSS16.0 was used for statistical analysis, and measurement data was used Indicates that the data conforming to normality and homogeneity of variance were analyzed by one-way analysis of variance, and the components were tested by paired t test, and the data not conforming to normali...
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Abstract
The invention discloses a method and a reagent kit for establishing an experimental model of a diabetic foot ulcer rat with mixed infection of Gram positive bacteria and Gram negative bacteria. The method comprises the following steps: after the rat is fed by a high fat feed for eight weeks, injecting streptozotocin to generate a diabetic model, after the diabetic model is stable, generating a scald with the depth of II degrees in the skin of the diabetic rat, and three days later, injecting mixed suspension of the Gram positive bacteria and the Gram negative bacteria at the scald. In addition, the invention also discloses the reagent kit for establishing the experimental model of the diabetic foot ulcer rat with the mixed infection of the Gram positive bacteria and the Gram negative bacteria. The reagent kit comprises the mixed suspension of the Gram positive bacteria and the Gram negative bacteria. According to the diabetic ulcer experimental animal model disclosed by the invention, by detecting the changes of biochemical indicators after drug intervention, results show that the model has the characteristic of being capable of generating the diabetic foot ulcer with various bacteria colony infections, and the characteristics of long healing time of an ulcer wound surface, sensitivity to drug reaction and the like.
Description
technical field [0001] The invention belongs to the field of experimental animal models, and in particular relates to a method and a kit for establishing an experimental model of diabetic foot ulcer rats infected with Gram-positive bacteria and negative bacteria. Background technique [0002] With the sharp increase in the incidence of diabetes in my country, the number of patients with diabetic ulcers has also increased. Establishing a disease model that truly simulates diabetic ulcers at the overall animal level is an important tool for medical research such as exploring the pathogenesis of diabetic ulcers and preclinical pharmacodynamic evaluation. It has very important scientific and clinical significance. [0003] At present, animal models of diabetic foot ulcers are mostly induced animal models. The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease,...
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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K31/7008A01K67/02
CPCY02A50/30
Inventor 柳国斌韩强
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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