84 results about "Experimental Animal Models" patented technology

The invention discloses a construction method of a Rosa26 gene Mir3061 fixed-point knock-in heterozygote mouse model and an application thereof. The constructed mouse gene is Rosa26LSL/+, and the construction method is provided for the first time and is not reported in any domestic or foreign literature reports. And the model provides an effective experimental animal model for the research on thepathogenesis of POF, the research and development of medicines and the evaluation of drug effect. The invention also provides the application of the mouse model in screening and preparing medicines for detecting/treating reproductive development diseases and medicines for detecting/treating aging and apoptosis diseases of ovarian granulosa cells. The model provides an effective experimental animalmodel for reproductive development genetics, preparation of medicines for detecting/treating ovarian diseases and screening of related medicines, and has good practical value, good medical clinical application prospect and great social benefit.

The invention provides a construction method and an application of an HBeAg transgenic mouse model. The method comprises the following steps: CRISPR/Cas9 technology is adopted, an HBeAg gene is inserted into a pliver-HBeAg expression cassette at the Rosa26 gene locus in a fixed-point manner through homologous recombination, and a recombinant R26-e(Alb-HBeAg)1 targeting vector is constructed with an In-Fusion cloning method and contains a 3.3kb 5' homologous arm, pliver-HBeAg and a 3.3kb 3' homologous arm; Cas9mRNA, gRNA and the recombinant R26-e(Alb-HBeAg)1 targeting vector are micro-injectedinto a zygote of a C57BL/6J mouse, then the zygote is transplanted into the uterus of a C57BL/6J female mouse, and an HBeAg transgenic mouse is obtained through propagation and purification step by step. The transgenic mouse can specifically express HBeAg in liver, thereby being capable of applying to experimental animal models for researching HBeAg infection mechanism and evaluating treatment effects of therapeutic drugs and vaccines for chronic hepatitis B.

The invention belongs to the field of biotechnology, and relates to an experimental animal model for transforming nonalcoholic steatohepatitis into liver cancer. In particular, a large and mouse model of nonalcoholic chronic steatohepatitis (NASH) and chronic steatohepatitis developing into chronic liver fibrosis, cirrhosis, and hepatocellular carcinoma (HCC) can be used for chronic fatty Liver, steatohepatitis, liver fibrosis, liver cirrhosis, hepatocellular carcinoma, metabolic diseases, diabetes, in vivo experiments of related mechanisms of diabetic complications, new drug development, and non-alcoholic steatohepatitis (NASH), liver It has extremely high application value in the study of the pathogenesis of fibrosis and liver cancer and in drug development.

The present invention relates to a normal pressure low oxygen exposure system, which is characterized in that the present invention includes: a box body, which is provided with an air inlet, an air outlet and an open-door; an airflow balance plate which is provided with a plurality of through holes and is arranged in the box body at the end of the air inlet; an oxygen cylinder with the concentration of 10 percent, an outlet of which is provided with a pressure reducing valve and a flowmeter, and the outlet is connected with the air inlet of the box body by a gas pipe; a container containing the substances which can absorb the carbon dioxide and a container containing the substances which can absorb the water vapor, which are respectively arranged in the box body; an oxygen sensor and a carbon dioxide sensor, which are respectively fixed in the box body; a PC mainframe and a display, which are connected mutually and are arranged at the outside of the box body; a signal amplifier, which is respectively connected with the PC mainframe, the oxygen sensor and the carbon dioxide sensor by guide wires. The present invention occupies less space and can monitor the concentrations of the oxygen and the carbon dioxide in the box body at any time, so the present invention can be widely used in the normal pressure and low oxygen exposure process for the preparation of the experimental animal models.

The present invention relates to the field of biotechnology and in particular to a construction method of an experimental animal model, specifically a mouse model, with non-alcoholic steatohepatitis (NASH) transforming into hepatic carcinoma (HCC), and use thereof. The present invention provides a construction method of a mouse model with NASH and chronic steatohepatitis developing into chronic hepatic fibrosis, cirrhosis and HCC. The construction method comprises the following steps: newborn C57BL/6 mice (including male and female newborn mice) are subjected to subcutaneous single injection of streptozotocin (STZ) (100-400 [mu]g/newborn mouse) and the mice begin to be fed with experiment use high-fat feed after the completion of female mouse breastfeeding to construct the NASH mouse model with hepatic fibrosis, cirrhosis and HCC. The provided mouse model is relatively simple in construction process, relatively simple in technical means, and low in construction cost, has an extremely high value as the needed animal model in scientific research and drug research and development, and belongs to a domestic initiative.

The invention provides a method for constructing a lung tumor experimental animal model. The method comprises the following steps of: 1, feeding a dog with cyclosporin according to the weight ratio of the dog to cyclosporin of 10mg to 1kg 4 days before inoculation; 2, preparing canine transmissible venereal tumor (CTVT) tissue blocks; and 3, puncturing to a predetermined target part by using a 16G guide puncture needle, placing 2 to 3 CTVT tissue blocks into the puncture needle, arranging a sterile gelatin sponge strip behind the CTVT tissue blocks, pushing the tissue blocks into lung tissues by a needle core and pulling the needle out. In the method, CTVT cells are used as tumor strains, the tumor tissue blocks are prepared through subcutaneous tumor cell passage by using the characteristics of transplantability and genetic stability; in-lung planting is performed by a minimally invasive intervention method to establish a dog transplantable lung cancer model; and the model can be applied to related experimental researches such as growth, invasion, transfer and the like of large-sized animal lung tumors.

The invention discloses a method for constructing a heterozygote mouse model of an Amh gene fixed-point knock-in 2A-Cre and an application thereof. According to the method, the heterozygote mouse model can be obtained, and an effective experimental animal model is provided for the research of POF pathogenesis, the research and development of drugs and the evaluation of drug effect. The invention provides the application of the heterozygote mouse model in screening and preparing drugs for detecting/treating reproductive development diseases, drugs for detecting/treating ovarian diseases and drugs for aging and apoptosis diseases of ovarian granulosa cells. The invention provides the method for constructing the heterozygote mouse model for the first time, and no domestic and foreign literature reports are found before. The model has good medical clinical application prospect, has great application value in preparing medicaments for detecting/treating ovarian diseases, and has great social benefit.

The invention relates to a cervical spondylotic myelopathy (CSM) experimental animal model and a making method thereof, belonging to the technical field of osteopathic medicine experimental animal models. According to the technical scheme of the invention, a living rabbit, goat, dog, miniature pig or experimental mouse is used as a model body, a compression nail is placed at C3 cervical vertebra body of the rabbit, goat, dog, miniature pig or experimental mouse, and developing biomaterial is filled inside the filling hole of the compression nail. The making method of the CSM experimental animal model comprises the following steps of: making a hole at the C3 cervical vertebra body, and placing the compression nail into the hole to cause spinal cord ventral compression. The CSM experimental animal model provided by the invention has the following benefits that the C3 cervical vertebra is selected as the vertebral body where the compression nail is placed, which completely breaks the domestic and abroad traditional mode of compression at C4 or C5 cervical vertebra, is a new breakthrough in CSM experimental animal model making and achieves an ideal experimental effect; and the surgical operation is simple and safe, and the change of CSM after long-term chronic compression can be studied in various imaging techniques at the same time.

The invention discloses a method for establishing an experimental model of a diabetic foot ulcer big mouse infected by staphylococcus aureus. The method includes the following steps that after the big mouse is fed on high-fat diet for eight weeks, streptozotocin is injected, and a diabetic model is manufactured. After the diabetic model is stabilized, II-degree scalding is performed on the skin of the big mouse, and after three days, subcutaneous injection of suspension liquid with staphylococcus aureus as the bacterial strain is performed in each ulcer position. In addition, the invention further discloses a kit for establishing the experimental model of the diabetic foot ulcer big mouse infected by the staphylococcus aureus, wherein the kit comprises the staphylococcus aureus suspension liquid. The diabetic foot ulcer experimental animal model detects variation of biochemical indexes after pharmacological intervention, and a result shows that the model has the infection features of diabetic foot ulcer and concurrent gram-positive bacteria. The model has the advantages of being short in ulcer wound surface healing time, sensitive to drug reaction and the like.

The invention provides a device and a method for measuring periphery muscle tension of a rat paw, wherein the measuring device provided by the invention comprises a paw fixing device, an expansion connecting device, a signal collecting device, a signal processing unit, a servo motor, a servo driver and a microprocessor, wherein the expansion connecting device is fixedly connected with the paw fixing device; the signal collecting device is respectively fixedly connected with the paw fixing device and the expansion connecting device and can be used for collecting voltage signals of the peripheral muscle tension of the paw; the signal processing unit is connected with the signal collecting device and can be used for converting the collected voltage signals to digital signals; the servo motor is fixedly connected with the expansion connecting device and can be used for driving the paw to rotate around an ankle; the servo driver is connected with the servo motor and can be used for controlling angular displacement and/or angular velocity in the rotation of a motor spindle; and the microprocessor can be used for generating corresponding electric signals according to preset angular displacement and/or angular velocity and sending the electric signals to the servo driver. According to the device and the method measuring the periphery muscle tension of the rat paw, the measurement on the periphery muscle tension of the rat paw is realized, and the technical problem that limb muscle tension of an experimental animal model cannot be measured accurately in the prior art is solved.

The invention provides a novel application of a recombinant ganoderma lucidum immunological regulation protein (rLZ-8) in treating thrombopenia. In the experiments of preventing and treating the thrombopenia of rats and dogs caused by cyclophosphamide, the rLZ-8 prepared from physiological saline carries out administration on an experimental animal model with lower thrombocytes, THPO and IL-6 are used as positive medicines, continuous administration is carried out, the number of the thrombocytes in blood is detected, the changes of the number of the thrombocytes before and after therapy are contrasted, and the medicine effect is analyzed. Compared with a model group, the rLZ-8 medicine group already obviously stimulates the thrombocyte proliferation of rats, Guinea pigs and dogs in the initial period (the 3rd day to the 5th day) of the administration, the difference is very obvious, the thrombocytes are recovered to a normal level in the middle period (the 7th day to the 10th day) of the administration, and the invention also obtains a very good effect in treating the thrombopenia of a Guinea pig experimental animal model caused by injecting anti-thrombocyte serum.

The invention relates to a method for selecting anti-hepatitis C medicine, which injects the artifical hepatitis C virus into tree mouse with negative hepatitis C, to build the hepatitis C natural infection model; via following steps as (1) composing hepatitis C virus; (2) injecting it into normal tree mouse, while the virus carried amount of each animal is higher than 105c; (3) fixing the hepatitis C virus naturally to infect the tree mouse model; (40 using the mouse model with positive hepatitis C virus to select the anti- hepatitis C medicine.

The invention relates to a sciatic nerve ligation subcutaneous tumor-bearing rat model and application of the sciatic nerve ligation subcutaneous tumor-bearing rat model in screening of analgesic and anti-tumor medicine. Single-side sciatic nerve ligation is conducted on an adult Wistar rat subjected to hypodermic implantation of Walker256 tumor cell suspension, the pain degree of the rat is reflected through spontaneous pain, the mechanical pain threshold value and the hot pain threshold value, and the tumor growth situation is indicated through the body weight change, the tumor formation rate and the tumor size of the rat. After the to-be-detected medicine is applied to the animal model according to the required mode and dosage, and the effect of the to-be-detected medicine on the aspect of analgesia and tumor prevention is judged by observing changes of the indexes. The experimental animal model which has the lasting and stable pain characteristic and can visually measure the tumor growth situation of the tumor of the rat is used for exploring the mechanism of promoting tumor growth through pain and used for screening the novel analgesic and anti-tumor medicine. The new carrier is provided for research and development of the novel analgesic and anti-tumor medicine.

The invention belongs to the technical field of vertebrate breeding, and discloses an anti-tumor experimental animal model and a construction method thereof. The right armpits of mice or rats for experiments are taken for subcutaneous vaccination of a mouse or rat tumor sterile diluent according to the sterile operation, and weighing and grouping are performed after 24 hours; weighing is performedafter 24 hours, random grouping is performed, 10 mice or rats fall into one group, 20 mg/kgi.p of CTX is applied every day, and intragastric administration is performed on the other groups; a constant amount of distilled water is given to a model control group, the equal amount of normal saline is inoculated on normal animals which are animals with no inoculated tumor as ten animals as a fake inoculation group, the constant volume of distilled water is fed every day to the normal animals as a normal control group, and the water is fed every day for 7 days. According to the animal experimentalmodel, a tumor sterile diluent is directly injected into experimental animals under the sterile condition, tumors grow inside the animals, the verification process is short, and by weighing the tumorweight and calculating the inhibition rate, the verification effect is convictive.

The invention discloses a modeling feed for constructing a hyperhomocysteinemia animal model and a preparation method thereof, and relates to the technical field of construction of medicine experimental animal model. The preparation method comprises the steps: according to the proportion, weighing casein, corn starch, sucrose, cellulose, DL-methionine, L-cystine, a composite mineral, a composite vitamin and choline dihydrogen citrate, vegetable oil and water; mixing the weighed materials, stirring uniformly, preparing feed particles by a hoop standard granulator, and drying the feed particles to obtain feed particles; and finally, carrying out irradiation sterilization by using cobalt 60, to obtain the finished product. The product is formed by single stable nutrients, the nutrient composition is not affected by seasons and regions and is stable, and the processing technology at normal temperature is adopted to ensure no change of the nutrient composition of the product. The model construction success rate is high, the model preparation process is simple, and the model formation effect is good.

The invention provides a traditional Chinese medicine compound for tranquilization, which comprises the following components: 10 to 100g of ligustrum lucidum, 10 to 100g of mulberry, 30 to 300g of caulis polygoni multiflori, and 25 to 250g of Herba Sedi Aizoon. The traditional Chinese medicine compound can futher comprise the components as follows: Salvia miltiorrhiza is 15 to 150g, Prepared Nutgrass Galingale Rhizome is 10 to 100g, and Xiangyuan is 10 to 100g. Adding and subtracting can be performed based on the components, and the traditional Chinese medicine compound can further comprise epimedium powder and Rhizoma Curculiginis or comprise tortoise plastron, rhizoma anemarrhenae and phellodendron. The traditional Chinese medicine compound shows the action equivalent to a climacteric-syndrome-relieving tablet in an experimental animal model and clinical cases, and can be used for preparing the drugs for treating climacteric syndrome.

The invention relates to the technical fields of construction of experimental animal models and laboratory instruments, and particularly relates to a construction method and construction equipment for an SCI (spinal cord injury) animal model. The construction equipment comprises an operation platform, uprights and a cross beam, wherein the four-limbs fixing clips are arranged on the upper surface of the operation platform; the uprights are arranged on the side edges of the operation platform, and connected with the cross beam, a lifting rod is vertically hung on the cross beam, and the bottom of the lifting rod is connected with an L-shaped pressing rod. The method and the equipment have the beneficial effects that during the clinic practical work, the original experimental method of striking and pressing from the ventral and from outside the of the spinal cord is changed into the method of pulling outwards from the inner side of the spinal cord of the experimental animal to stimulate the spinal cord compression injury degree, so that the spinal cord injury situation of people is better stimulated.

The invention belongs to the technical field of experimental animal models, and provides a method for constructing a rat model with pine cone injury, which aims to solve the problems of complex operation, low success rate, long modeling time, low repeatability and the like of the conventional rat model with pine cone injury. The method comprises the following steps of: performing intraperitoneal injection of PCPA of 450 mg/kg on a rat for 2 days continuously, once every day; or performing electrical stimulation on the rat by using a shuttle box, wherein the electrical stimulation voltage is 30V, the current is 0.8A, the stimulation time is 30 seconds, the interval is 30 seconds, the cycle is performed for 60 times, and the operation is performed for 5 days continuously; or performing an intraperitoneal injection PCPA method on the rat on the fourth day by using an electrical stimulation method. Three modeling methods, namely pathological histomorphology, serum detection of the contentof melatonin which is a main synthetic secretory hormone of a pine cone, and animal general situation and behavioral auxiliary method objective evaluation, cause injury of the pine cone of the rat, and provide a good model basis for researching and screening medicaments and mechanisms with protective and therapeutic effects on the pine cone injury; and the method is simple and convenient, the modeling time is short, the repeatability is high, and the index evaluation is simple and reliable.

The invention relates to establishment of a rhesus monkey immunosuppression model, and provides an application of cyclophosphamide in preparing the rhesus monkey immunosuppression model. Specifically, cyclophosphamide with different dosages are selected to carry out intravenous injection on the rhesus monkey, then the suitable immunosuppression molding dosage is determined, and a disease model consistent with the variation tend of various immune indices under human immunosuppression state is established, wherein the optimal use method and the optimal use amount of cyclophosphamide are as follows: intravenous injection is carried out at 15mg/kg each day. The invention further provides a method for preparing the rhesus monkey immunosuppression model, and an immunosuppression animal module prepared by the method. The animal mold can be used for the fields of biotechnology new drug research and development, immunosuppression mechanism study, virus infection modeling and the like, which can not be completed by rodent model, and provides a more ideal and more reliable experimental animal models and a perfect evaluation system for the above application compared with the rodent model.

The invention discloses a method for establishing an experimental model of a diabetic foot ulcer big mouse infected by Escherichia coli. The method includes the following steps that after the big mouse is fed on high-fat diet for eight weeks, streptozotocin is injected, and a diabetic model is manufactured. After the diabetic model is stabilized, II-degree scalding is performed on the skin of the big mouse, and after three days, subcutaneous injection of Escherichia coli suspension liquid is performed in each ulcer position. In addition, the invention further discloses a kit for establishing the experimental model of the diabetic foot ulcer big mouse infected by the Escherichia coli, wherein the kit comprises the Escherichia coli suspension liquid. The diabetic foot ulcer experimental animal model detects variation of biochemical indexes after pharmacological intervention, and a result shows that the model has the infection features of diabetic ulcer and concurrent Gram-negative bacteria. The model has the advantages of being short in ulcer wound surface healing time, sensitive to drug reaction and the like.

The invention belongs to the technical field of biology, relates to a bone inflammatory pain model, in particular to application of a complete Freund adjuvant (CFA) to the preparation of a tibia inflammatory pain model. Tests on the tibia histopathology and behavioral indicators such as a heat pain threshold, a mechanical pain threshold and the like of the bone inflammatory pain model prepared by the CFA prove that: the inflammation of an experimental model is obvious, and the level of the behavioral indicators is low. A valuable bone inflammatory pain contrast model is provided for the research of bone cancer pain, particularly the mechanism research of cancer pain; a valuable experimental model is provided for the research of pain caused by clinical osteomyelitis; meanwhile, the type of a chronic pain experimental animal model is enriched, and the prepared tibia inflammatory pain model has important academic research value and application prospects.

The invention discloses a short peptide blocking long-term synaptic potentiation (LTP) and application thereof and belongs to the field of biological chemistry. A bioinformatics method is adopted to analyze human homologous sequences of different species of AMPA receptor subunits GluR2, it is found that a high conserved domain exists at the terminal C of GluR2, and the short peptide is designed on the basis and further comprises a short peptide modification peptide having cytomembrane penetrating capacity and a short peptide modification peptide marked with a fluorescent group. Amino acid sequences of the short peptide are shown as SEQ ID NO.1 and SEQ ID NO.2. The short peptide or the short peptide modification peptide can block LTP expression and has no influence on functions of LTD and NMDA receptors having synaptic plasticity. Therefore, the short peptide or the short peptide modification peptide is applied to preparation of drugs for treating or preventing diseases related to glutamic acid overexcitation diseases and pathological synaptic plasticity LTP, or establishment of experimental animal models of the diseases related to glutamic acid overexcitation diseases and the pathological synaptic plasticity LTP.

The invention discloses an establishing method and application of an asthma-like chronic obstructive pulmonary overlap airway inflammation mouse model. The model is constructed by any one of the following modes: S1, smoking and aspergillus protease induced ACO-like model: enabling mice to inhale smoke of cigarettes every day from the 0th day; after smoking treatment is completed for 3-7 days, enabling an aspergillus protease solution to be dropwise added into the noses to sensitize the mice, wherein an ACO-like model is constructed; S2, preparing an ACO-like model induced by nano carbon blackparticles and aspergillus protease: intranasally dripping the nano carbon black particles into the anesthetized mice from the 0th day, wherein after the nano carbon black particles are treated for 2 weeks, an aspergillus protease solution is dropwise added into the noses to sensitize mice, and an ACO-like model is constructed. The constructed ACO-like model can simulate the characteristic that asthma chronic obstructive pulmonary overlap occurs in the later stage of diseases of patients with chronic obstructive pulmonary disease clinically, and an experimental animal model is provided for ACOmorbidity mechanism and drug treatment research.