31 results about "Experimental Animal Models" patented technology

The invention relates to the technical field of disease animal models, in particular to a construction method, an evaluation method and application of a bipolar affective disorder liver stagnation and spleen deficiency syndrome combined animal model. According to the method, a liver depression and spleen deficiency syndrome modeling method is adopted for model construction, evaluation indexes are formulated by combining manic and depression behavior expressions of mice and innovatively combining typical expressions of liver depression and spleen deficiency, a liver depression and spleen deficiency syndrome animal model evaluation method is explored, and a thought is provided for achieving standardization and normalization of liver depression and spleen deficiency syndrome model evaluation. The modeling method is simple and economical, provides an experimental animal model for syndrome differentiation treatment of diseases, particularly provides a corresponding animal model for traditional Chinese medicine syndrome differentiation treatment and new drug pharmacological experiments, accelerates the progress of traditional Chinese medicine disease syndrome research and new drug development, provides technical service for drug evaluation, and has great application value.

The invention belongs to the technical field of medical experimental animal models, and particularly relates to a construction method of a rabbit ear acne model for studying acne pathogenesis and drug curative effect. A healthy New Zealand large-ear white rabbit is selected, a testosterone solution is subcutaneously injected to induce endocrine disorder, oleic acid is smeared at an opening of an external auditory canal on the inner side of an ear, dermatobacterium acnes suspension is subcutaneously injected, and whether acne-like lesions such as erythema, papule and comedo appear on the skin of the ear or not is observed. The method is easy and convenient to operate, low in cost, good in repeatability, capable of simulating the pathological process of human acne and suitable for acne pathogenesis research and drug screening.

The invention belongs to the technical field of medical experimental animal models, and discloses a small animal model for skull base high-flow cerebrospinal fluid leakage and skull base reconstruction as well as a construction method and application of the small animal model. The technical obstacle that due to the fact that the nasal cavity of a small animal is narrow and the turbinate structure is complex, surgical instruments cannot reach the skull base through the nose is overcome, meanwhile, a modeling and repairing area is arranged on the rear nasal meatus and the top wall of the nasopharynx, and the temperature and humidity conditions and environment of the nasal mucosa are reserved; and the microenvironment for tissue healing after the endoscopic transnasal skull base surgery is restored on the pathophysiological level. The dissected marking points are clear, and compared with narrow anterior skull base low-flow cerebrospinal fluid leakage of small animals, the standardized bone defect constructed in the middle skull base butterfly saddle area can cause continuous and high-flow cerebrospinal fluid leakage; autologous bone flap and free mucous membrane repair is used, the method is the same as current clinical practice, and a new platform can be provided for screening of skull base repair materials or operation evaluation and retrograde intracranial infection research after an operation.

The invention belongs to the technical field of experimental animal models, and particularly relates to a construction and analysis method of a zebra fish heart failure model. The invention discloses a construction method of a zebra fish heart failure model. The construction method comprises the following steps: S1, pretreating a zebra fish embryo; and S2, putting the zebra fish embryos pretreated in the step S1 into an exposure solution for exposure, thereby completing the construction of the zebra fish heart failure model. According to the construction and analysis method of the zebra fish heart failure model, accurate induction and dynamic monitoring of heart failure phenotypes are achieved through a standardized drug treatment process and a multi-mode heart function evaluation system, and an efficient platform is provided for cardiovascular drug screening and toxicity evaluation. The construction and analysis method of the zebra fish heart failure model further extends to the field of medicine and functional food heart protection efficacy evaluation, and objective grading authentication of food and medicine efficacy is achieved.

The invention belongs to the technical field of medical experimental animal models, provides a construction method of a dry age-related macular degeneration animal model, and aims to break through the technical bottlenecks of long construction period, high cost and incomplete pathological feature coverage of an existing model. D-galactose is used for inducing glycosylation end product accumulation and mitochondrial dysfunction, oxidative stress, RPE metabolic disorder and inflammation microenvironment in the dry AMD pathogenesis process can be synchronously simulated, core pathological characteristics such as retina glass membrane wart deposition, RPE atrophy, retina and visual function damage and the like can be accurately reproduced, meanwhile, a multi-dimensional verification system is matched, and the method has the advantages that the method is simple and convenient to operate and high in practicability. The method is suitable for preclinical evaluation of personalized treatment strategies; besides, the method is short in modeling period, the animal model meeting the requirement can be obtained within 8 weeks, the cost advantage is outstanding, and an efficient and reliable experimental animal model can be provided for dry AMD treatment research.

This invention discloses a method for establishing a mouse model of outer retinal tubular structures, which is obtained by specifically knocking out the Lss gene in the mouse retina. This invention utilizes CRISPR / Cas9 gene editing technology to construct a mouse model of outer retinal tubular structures by specifically knocking out the Lss gene in the retina. The mouse model of outer retinal tubular structures constructed by this method is similar to the clinical phenotype of this type of retinal disease, providing a relatively ideal experimental animal model for studying the developmental regulation of the mammalian retina and the pathogenesis of retinal degeneration.

The invention relates to the technical field of experimental animal models, in particular to an establishment method and application of a rat radioactive cataract model. According to the invention, a rat cataract model induced by irradiation of X-rays with different doses (10 Gy, 20 Gy and 30 Gy) is constructed. Through multi-dimensional evaluation such as general situation observation, slit lamp inspection and histopathologic analysis, the influence of ionization radiation (IR) on the crystalline lens is systematically observed. The animal model successfully reproduces pathological manifestations of different radiation-damaged crystalline lenses, and an experimental basis is provided for further research on pathogenesis of radiation-induced cataract.

The invention discloses an application of a NanoLuc luciferase double-subunit complementary system in construction of an experimental animal model. The genome of the experimental animal model contains a NanoLuc enzyme large subunit LgBiT encoding gene and stably expresses LgBiT; correspondingly, a tested object used for invading the experimental animal model or connected into the experimental animal model is in fusion connection with NanoLuc luciferase small subunit HiBiT polypeptide serving as a label or a NanoLuc luciferase small subunit HiBiT coding gene serving as a reporter gene, and LgBiT and HiBiT are specifically combined in the experimental animal model and recombined to form the NanoLuc enzyme with catalytic activity. A strong bioluminescence signal is generated under the action of a furazine substrate, so that high-sensitivity, non-invasive and real-time dynamic imaging of in-vivo distribution, loading capacity and diffusion path of a detected object is realized. The invention also discloses a gene editing system for constructing the LgBiT transgenic mouse.

The invention relates to the technical field of experimental animal model construction in biomedical research, and discloses a common bile duct flow limiting tube for constructing a chronic cholestasis rat model and a construction method, the flow limiting tube comprises a hard tubular body and a soft protective fixing wing wrapping the tubular body, the inner diameter of the tubular body is 0.12-0.15 mm, and the outer diameter of the tubular body is 0.12-0.15 mm. The length of the tubular body is 0.5 to 0.8 cm; protruding fixing rings are arranged at the two ends of the protection fixing wing, and the total outer diameter of the flow limiting pipe is smaller than or equal to 0.7 mm. The common bile duct flow limiting tube is implanted into the specified position of the common bile duct of an experimental animal through a precise operation, precise control over bile flow is achieved, and therefore a standardized animal model capable of highly simulating the human chronic cholestatic liver disease in the aspects of the pathological process, physiological compensation and systematic expression is established. The model and the construction method thereof can be directly applied to multiple directions of liver pathophysiology mechanism research, drug target verification, new drug curative effect and safety evaluation and the like.

The invention relates to the technical field of medical experimental animal models, in particular to a construction method of an animal model for researching jaw cyst, which comprises the steps of experimental animal selection, surgical site preparation, bone windowing operation, cyst induction material implantation, wound suture, postoperative care and observation, and simulation of in-vivo development of jaw cyst by an implant. The invention provides a jaw cyst animal model which is high in modeling success rate, good in stability, highly similar to pathological characteristics of a bone cavity of a human jaw cyst and simple and convenient to operate, and a construction method of the jaw cyst animal model. A reliable experimental model is provided for pathogenesis research, pathological process discussion, drug research and development, treatment method evaluation and the like of the jaw cyst.

The invention discloses a micro-plastic exposure health risk detection method with desulfovibrio as a biomarker. The method comprises the steps of fecal sample collection, DNA extraction, desulfurization vibrio abundance detection and risk judgment. Based on the experimental conclusion that microplastic exposure can significantly enrich intestinal desulfurization vibrio, and the abundance of the bacterium is positively correlated with neuropathy and cancer related metabolites, 16S rRNA sequencing is adopted to detect the abundance of the desulfurization vibrio, and health risk assessment is achieved through comparison with a control group. The method has the advantages of noninvasive property, specificity and convenience, can be used for human microplastic exposure screening or experimental animal model research, and provides an effective tool for microplastic health risk early warning.

The application relates to an induced type 2 diabetes mellitus feed and application thereof in establishing a type 2 diabetes mellitus animal model, and relates to the field of experimental animal models, and particularly relates to an induced type 2 diabetes mellitus feed and application thereof in establishing a type 2 diabetes mellitus animal model. The application aims at solving the problems of long modeling time, instability and easy change into a type 1 diabetes mellitus model of the existing type 2 diabetes mellitus animal model establishing method. The feed comprises desulfurization vibrio powder, symbiotic clostridium powder and high-fat high-sugar feed. The method comprises the following steps: drying desulfurization vibrio liquid and symbiotic clostridium liquid into powder, uniformly mixing the powder and the high-fat high-sugar feed, and extruding into a column shape by using an extrusion molding machine. The application significantly shortens the establishment time of the type 2 diabetes mellitus animal model, does not need to inject a high-dose STZ, and does not cause the type 1 diabetes mellitus model of absolute insulin deficiency of rats in the case of using a higher concentration of STZ induction. The induced type 2 diabetes mellitus feed is used for establishing the type 2 diabetes mellitus animal model.

The application provides a construction method of a zebrafish model of diabetic cerebral hemorrhage sequelae for high-throughput drug screening, and belongs to the technical field of biotechnology.The first zebrafish model capable of comprehensively simulating the complex pathological phenotypes of clinical diabetic cerebral hemorrhage sequelae is successfully constructed, and the blank of the research model in the field is filled.The pathological changes of the model are highly consistent with the symptoms of the patients with clinical diabetic cerebral hemorrhage sequelae, such as glucose metabolism disorder, pancreatic beta cell damage, cerebral vascular injury and hemorrhage, brain cell apoptosis, inflammatory reaction and behavior abnormality, and the limitation that the existing single disease model cannot simulate the synergistic pathogenesis of diabetes and cerebral hemorrhage is overcome, so that a reliable experimental animal model is provided for high-throughput screening of active ingredients against the disease.

This invention belongs to the field of biomedical experimental animal model construction technology, specifically relating to a method for constructing a rat model of radiation-induced recurrent dermatitis (RRD). This invention successfully induces recurrent skin inflammation localized to the original irradiated area in rats that have undergone initial radiation injury by inducing drug therapy at a specific induction starting point, thus obtaining a rat model of RRD. The model constructed by this method successfully reproduces the recurrent characteristics of RRD, with inflammation only occurring in the previously irradiated area, while the unirradiated skin remains intact, exhibiting good clinical fit and stability. This model provides a reliable in vivo experimental platform for in-depth research into the pathogenesis of RRD, screening intervention targets, and evaluating treatment regimens.

The invention belongs to the technical field of medical experimental animal models, and particularly relates to a stable and high-reproducibility severe acute pancreatitis mouse model construction method, a quantitative evaluation system and application thereof, and the method comprises the following steps: selecting a male C57BL / 6 mouse of 6-8 weeks old, adaptively feeding for one week, and then carrying out model induction; the preparation method comprises the following steps: carrying out intraperitoneal injection on leptofrog at the dosage of 100 mu g / kg once every 1 hour, and continuously injecting for 7 times; within 2 hours after completion of the seventh time of rain frog element injection, lipopolysaccharide is injected into the abdominal cavity, and the dosage is 12 mg / kg; pathological and physiological index evaluation is carried out on the mouse 24 hours after the first time of rain frog element injection, and multi-batch verification experiments prove that the inter-batch fluctuation range of the model constructed by the invention on the death rate, the pathological score, the weight change and other key indexes is controlled within + / -5%, so that the repeatability and comparability of experimental results are ensured.

The invention belongs to the technical field of medical experimental animal models, and particularly relates to a construction method of a spleen and stomach damp-heat syndrome chronic atrophic gastritis animal model. The construction method comprises the following steps: firstly, performing spleen and stomach damp-heat modeling on a tested animal to obtain spleen and stomach damp-heat syndromes, and then performing chronic atrophic gastritis induction. According to the specific three-step atrophic gastritis induction method, the clinical pathological process of inflammation caused by stimulation, gastric mucosa atrophy caused by damp heat and blood stasis for a long time and intestinal metaplasia can be accurately copied, the clinical pathological process is highly matched with the stage progress characteristic of clinical chronic atrophic gastritis, and the model is high in success rate, good in stability and high in repeatability.

The invention relates to the technical field of experimental animal model construction, and particularly discloses an ulcerative dermatitis mouse model construction method which comprises the following steps: firstly, feeding a mouse with a high-fat feed A for 4 weeks; in the fifth week, the mixed feed of the high-fat feed A and the high-fat feed B lacking the vitamin B7 is replaced for feeding; from the sixth week to the twelfth week, high-fat feed B lacking vitamin B7 is used for feeding; in the sixth week to the tenth week, the mice are placed in a smoking device to be smoked for 2-3 h every day; in the tenth week to the twelfth week, vancomycin with the concentration of 100-120 mg / ml is injected into the tail vein of the mouse, the injection frequency is 2-3 times per week, and the injection amount is 40-60 mg / kg. The construction method of the ulcerative dermatitis mouse model provided by the invention can accurately simulate the pathogenesis of human ulcerative dermatitis, and provides a reliable experimental basis for researching the pathogenesis and treatment means of the ulcerative dermatitis.

The present application belongs to the field of biomedical technology, and relates to a construction method of an experimental animal model and a constructed experimental model, in particular to a method for constructing a kidney humanized mouse model based on human cells and a constructed kidney humanized mouse model. The construction method comprises the following steps: injecting mesenchymal stem cells, human umbilical vein endothelial cells and human renal tubular epithelial cells separated from urine into immunodeficient mice subcutaneously or subcapsularly to obtain a humanized kidney vascular unit mouse model communicating with the host. The model construction has the following characteristics: the cells used are derived from urine, have the characteristics of sufficient clinical source and convenient sampling, and the kidney source cells derived from urine can represent a unique individual alone, can be used to establish an individualized kidney humanized mouse model based on patients, and have the potential to be applied to individualized precision medical research; the model can form a functional human kidney vascular unit in mice, and has great application prospects in individualized precision medical treatment of kidney disease and human kidney specific virus infection research.

The application discloses an experimental animal modeling auxiliary device, which comprises a feeding cage, a feeding box is slidably installed on the inner side of the feeding cage, a partition plate is fixedly installed in the feeding box, the partition plate divides the feeding box into a feed cavity and a water cavity, a height adjusting mechanism is arranged below the feeding box, and the height adjusting mechanism is used for adjusting the height of the feeding box; a plurality of threaded grooves are formed in the outer wall of the feeding box, a restraint part is detachably installed in the threaded grooves, and the restraint part is used for fixing the forelimbs of the feeding animal; a pressurizing mechanism is arranged on the top of the feeding cage and is correspondingly arranged with the restraint part, and the pressurizing mechanism is used for increasing the weight of the feeding animal. The application simulates the state of the human knee joint from two aspects of mechanical characteristics and motion characteristics, thereby being closer to the pathogenesis characteristics of human knee osteoarthritis, better researching the pathogenesis and process of knee osteoarthritis, and better assisting the modeling of knee osteoarthritis of rats, so that the modeling is more scientific and reasonable.

The invention discloses a construction method of a type-2 diabetes mellitus with periodontitis mouse composite model, and belongs to the technical field of medical experimental animal model construction. Db / db mice are selected to obtain a spontaneous type 2 diabetes mellitus model which does not need to be induced and is stable in phenotype, if fasting blood-glucose is larger than or equal to 11.1 mmol / L for 12 hours, the type 2 diabetes mellitus model is successfully constructed, on the basis, the type 2 diabetes mellitus with periodontitis model is constructed through a silk thread ligation method, and short-term rapid modeling can be achieved only in four weeks. The composite model is low in construction cost and efficient, can be standardized, and provides a convenient and ideal animal model for researching the association and mechanism between the two diseases of type 2 diabetes and periodontitis.

This invention belongs to the field of experimental animal model construction technology, specifically relating to the construction method and application of a recombinant inbred type 2 diabetic rat model. The parent rats are SD (fa+ / +) or Wistar (fa+ / +) females and Zucker (fa+ / -) males. Heterozygous (fa+ / -) female offspring are backcrossed with the fathers until the F6 generation. Then, F6 heterozygous (fa+ / -) female rats are crossed with F6 heterozygous (fa+ / -) male rats. The resulting homozygous (fa- / -) SD / Zucker or Wistar / Zucker rats are the recombinant inbred type 2 diabetic rats. The rats obtained by this invention do not express functional leptin receptors, exhibit hyperphagia, and spontaneous polydipsia and polyphagia, which closely resembles the real-world scenario of type 2 diabetes caused by overnutrition under normal dietary conditions in humans.

The invention belongs to the technical field of experimental animal model construction, and particularly relates to a method for constructing an arteriosclerosis mouse model by using humanized flora. The technical problem to be solved by the invention is to establish an arteriosclerosis phenotype humanized mouse model taking intestinal flora of a patient with coronary artery disease as a donor source. According to the technical scheme, the method for constructing the arteriosclerosis mouse model by using the humanized flora comprises the following steps: collecting an excrement sample of a patient diagnosed as arteriosclerosis, and preparing a flora suspension; the sterile mouse is subjected to gavage, gavage is conducted once every other day, and gavage is conducted five times; lavage is carried out once a week; and the whole process lasts for 10-13 weeks from the beginning of gavage. The mouse model constructed by the method can systematically evaluate key pathological phenotypes of hypercholesteremia, vascular dysfunction, immune activation and the like of the mouse.

The method for screening key genes, metabolites and pathways of spinal cord injury based on multi-omics joint analysis comprises the following steps: (1) establishing an experimental animal model of spinal cord injury, setting a spinal cord injury group and a sham operation group, and observing the motor function of the injury side of the experimental animal by motor function score; (2) extracting spinal cord tissue samples of each group, performing histological detection by HE staining and Nissl staining, performing transcriptome sequencing by RNA-seq technology, and performing metabolome sequencing by LC-MS / MS technology; (3) applying bioinformatics to analyze the biological significance of a single omics; (4) integrating transcriptome data and metabolome data for joint analysis, exploring key genes, metabolites and pathways affecting the pathophysiological mechanism of spinal cord injury, and constructing a gene-metabolite network interaction diagram. The molecular mechanism affecting the pathophysiological process of spinal cord injury and the relationship between injury microenvironment genes and metabolites are disclosed, which provides a research basis for multi-omics joint analysis and action of spinal cord injury.

The invention relates to the field of experimental animal model construction, and discloses a method for improving the stability of a pigeon hyperuricemia model, which comprises the following steps: selecting and monitoring: selecting healthy adult pigeons, and obtaining individual metabolic parameters through pharmacokinetic experiments and circadian rhythm monitoring; the pigeons are placed in a controllable environment to be bred, the initial dosage of an inducer is calculated through a space-time coupling model on the basis of individual metabolic parameters, and the inducer comprises a uric acid precursor substance and a uricase inhibitor; and optimization induction: according to the initial dose, giving an inducer to the pigeons in stages, optimizing the dose of the inducer in real time in combination with a predictive control algorithm, and inducing the pigeons to generate hyperuricemia. Through combination of a space-time coupling model in a controllable environment, individual metabolic parameters are converted into adaptive initial doses, neglect of fixed doses on individual differences is avoided, and through staged administration and combination of a predictive control algorithm, the doses are dynamically adjusted to compensate metabolic fluctuations.

The invention belongs to the technical field of experimental animal models, and particularly discloses a method for establishing a rat osteoporosis model by using a sleep deprivation technology, which comprises the following steps of: a, randomly dividing rats into a normal control group and a sleep deprivation group; b, a specially-made sleep deprivation box is adopted for conducting sleep deprivation on the rats in the sleep deprivation group continuously for 14-28 days and 16-20 h every day, and the sleep deprivation box monitors the sleep state of the rats and starts an LED lamp and a sound device to conduct acousto-optic stimulation awakening when the rats fall asleep; feeding the rats in the normal control group in a common cage; and c, determining that the osteoporosis model is successfully established by detecting bone mineral density, bone microstructure, bone tissue morphology and / or bone metabolism biochemical indexes. The method is simple and convenient to operate, non-invasive and small in trauma, can effectively simulate the pathological process of osteoporosis caused by long-term sleep disorder, and provides a new experimental model for related research.

The utility model discloses an anti-radiation transfer draw-bar box after radioactive experimental animal modeling, which comprises a draw-bar box main body, an experimental animal storage component is arranged in the draw-bar box main body, the whole draw-bar box main body is rectangular, and three first zipper opening doors are equidistantly arranged on one side of the draw-bar box main body from top to bottom; three spaced grid plates are arranged in the draw-bar box main body at equal intervals from top to bottom, a collecting box is arranged at the bottom end of each spaced grid plate, and a plurality of air holes are formed in the two sides of the draw-bar box main body and located above the first zipper opening door. According to the utility model, the device for transferring the experimental animal is arranged to be in a draw-bar box shape, so that the device can be conveniently carried through the draw bar and the universal wheels at the bottom end when being transferred or carried; the first opening door at the side part of the draw-bar box is convenient for putting in, taking out and feeding experimental animals, the transparent observation window can be used for observing the animals, and meanwhile, the collection box in the draw-bar box is used for collecting animal wastes, so that unified treatment is facilitated.

This invention relates to a progressive pathological animal model for drug evaluation and its construction method, belonging to the field of experimental animal model technology. Addressing the shortcomings of existing models, such as their inability to simulate progressive clinical disease courses, low model success rate, large individual variability, and low fit with clinicopathological features, this invention employs a sequential three-stage synergistic induction modeling method. This method sequentially induces basal metabolic abnormalities, subclinical cardiorenal injury, and progressive cardiorenal fibrosis, accurately matching the natural course of human diseases. This method is simple to operate, requires no complex surgery, and produces a model with a high model success rate, low mortality rate, good uniformity, and a high degree of fit between pathological features and clinical findings. It can be used for screening drugs for the treatment of this disease, evaluating drug efficacy, and studying the mechanism of action.

The invention relates to the technical field of biomedical experimental animal models, in particular to a drug induction-based miniature pig diabetes mellitus model construction method and application. The drug-induced miniature pig diabetes model construction method comprises the following steps: mixing a citric acid solution with a sodium citrate solution to obtain a mixed solution, and adjusting the pH value of the mixed solution to 4.2-5.0 to obtain a citric acid buffer solution; under the dark condition, streptozotocin is dissolved in the citric acid buffer solution, and a streptozotocin solution is obtained; the prepared streptozotocin solution is injected into the ear edge vein of the fasting miniature pig at a time, and construction of the miniature pig diabetes mellitus model is completed; the injection dosage in the step S3 is 140 to 160 mg / kg. The construction steps of the miniature pig diabetes model are simplified, the long-term stable miniature pig diabetes model can be constructed, and a simpler, more convenient and more stable model construction method is provided for research on a miniature pig diabetes disease mechanism and a treatment method.

The present invention relates to the technical field of drug screening, in particular to a drug screening method and apparatus based on the therapeutic index, and an electronic device. The present invention combines two key pharmacodynamic evaluation indicators, i.e. survival rate and body weight, with respect to experimental animal models. Body weight data is ingeniously converted into data of the area on a relative body weight curve, which then further undergoes synchronous normalization with survival rate data, and a calculation formula for the therapeutic index is established to be used for drug screening. The drug screening method of the present invention can organically combine survival rate and body weight data to objectively reflect disease progression and prognosis outcomes, thus effectively amplifying the effect differences between different drug treatment groups, more accurately, conveniently and comprehensively screening out drugs having excellent comprehensive effects, greatly reducing the development cost for new drugs, and further providing more accurate data support for clinical and experimental researches.

This invention relates to the field of medical experimental equipment technology and proposes a sleep apnea-hypopnea disease modeling system, comprising an experimental animal, a sealed enclosure, a main control unit, a communication unit, an EEG / EMG monitoring circuit, and a valve control circuit. The experimental animal is placed in the sealed enclosure, which has an exhaust port. The input terminal of the EEG / EMG monitoring circuit is connected to the head of the experimental animal, and the output terminal of the EEG / EMG monitoring circuit is connected to the main control unit. The main control unit communicates with a host computer via the communication unit. The input terminal of the valve control circuit is connected to the main control unit, and the output terminal of the valve control circuit is connected to a first solenoid valve controlling a first gas cylinder and a second solenoid valve controlling a second gas cylinder. This technical solution solves the problem in related technologies where OSAHS experimental animal models cannot accurately simulate the clinical OSAHS disease process.