Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of Pseudomonas aeruginosa with broad-spectrum antibacterial activity and its application

A technology of Pseudomonas chloropinus, antibacterial activity, applied in the direction of application, bacteria, fungicide, etc., to achieve long-lasting antibacterial effect

Active Publication Date: 2019-12-31
SHANDONG YANTAI AGRI SCI & TECH INST
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In my country, Pseudomonas chloropinus is mainly used for the effective control of diseases caused by pathogenic fungi and bacteria in various vegetable crops, while Pseudomonas chloropinus against fungal diseases of fruit trees and field crops has not been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Pseudomonas aeruginosa with broad-spectrum antibacterial activity and its application
  • A kind of Pseudomonas aeruginosa with broad-spectrum antibacterial activity and its application
  • A kind of Pseudomonas aeruginosa with broad-spectrum antibacterial activity and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Obtaining of Pseudomonas chloropinus YTBTa14 Strain

[0038] First, collect healthy dandelion plants from the field, wash the surface with tap water and dry them, then take the roots, stems, and leaves for surface disinfection, add 10 ml of pure water to grind, let stand for 10 minutes, take 80ul supernatant, dilute Spread on NA solid medium and culture in a 28°C incubator for 3-7 days. Pick a single colony according to the characteristics of the colony shape, color, etc., and store it on the slant medium after purification.

[0039] Thirdly, Pseudomonas chloropinus YTBTa14 strains that antagonize various diseases of fruit trees and field crops were screened by the plate confrontation culture method. Cultivate apple ringworm, grape anthracnose, grape white rot, cherry phytophthora, and wheat sheath blight on a special medium, and after the pathogens are overgrown on the plate, use a puncher to punch out the bacteria on the edge of the plate. Then put it in the center ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to Pseudomonas chloropinus with broad-spectrum antibacterial activity and the application of the strain in the prevention and control of fruit tree and field crop diseases, belonging to the field of biotechnology. A kind of Pseudomonas aeruginosa with broad-spectrum antibacterial activity, classified as Pseudomonas aeruginosa ( Pseudomonas chlorophis ), the strain number is YTBTa14, the deposit number is CCTCC NO:M 2016099, and the 16S rDNA sequence is shown in the sequence table. The Pseudomonas chloropinus with broad-spectrum antibacterial activity of the present invention has strong broad-spectrum antibacterial activity against fungal diseases of fruit trees in both its thallus and fermentation liquid, and has a long-lasting and stable antibacterial effect. The great potential of broad-spectrum biocontrol fungicides provides a new way for the development of microorganisms for biocontrol.

Description

technical field [0001] The invention relates to Pseudomonas chloropinus with broad-spectrum antibacterial activity and the application of the strain in the prevention and control of fruit tree and field crop diseases, belonging to the field of biotechnology. Background technique [0002] Wheat and fruit trees are important economic crops in the world. They are harmed by many diseases all the year round, and the annual economic losses caused by diseases are difficult to estimate. At present, pesticides are one of the most effective means of preventing and controlling pests and diseases, but various problems caused by the widespread use of chemical pesticides have also followed, such as pesticide residues, environmental pollution, and the emergence of resistance to pests and diseases, which are not conducive to the development of agriculture. sustainable development. Therefore, the prohibition and restriction of the use of a large number of highly toxic and high residue pesti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/38
CPCA01N63/10C12N1/20C12N1/205C12R2001/38
Inventor 李宝燕王英姿王培松
Owner SHANDONG YANTAI AGRI SCI & TECH INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com