36 results about "Re-epithelialization" patented technology

The invention discloses a photo-thermal composite hydrogel patch and a preparation method and application thereof.The hydrogel patch is of a three-dimensional latticed porous structure formed by taking methylacryloyl gelatin (GelMA) and methylacryloyl hyaluronic acid (HAMA) as matrixes, loading niobium carbide nano-particles (Nb2CNPs) and vinipofen (VP) and conducting 3D printing or casting molding, the hydrogel patch is named as VP-Nb2C-coated GelMA / HAMA (VNGH) hydrogel patch for short. The VNGH has good photo-thermal antibacterial property, oxidation resistance and reepithelization promoting function, can significantly improve the quality of mouse diabetic wound healing, and provides a new thought and strategy for realizing scar-free healing of diabetic wounds.

The present invention relates to the technical field of diabetes, and in particular to a controlled-release diabetic wound healing promoting treatment method, comprising the following treatment experimental steps: step S1: preparation of Hst1@PNPs; step S2: Coomassie brilliant blue staining; step S3: scanning and transmission electron microscopy detection; in the present invention, a slow-release Hst1 treatment method is constructed by encapsulating Hst1 into platelet membrane vesicles derived from autologous blood. On the one hand, the PNPs encapsulate the linear Hst1 peptide structure to prevent it from being rapidly degraded by high proteases in diabetic wound exudate, thereby improving bioavailability. On the other hand, the PNPs can adsorb excess LPS in the wound exudate, and cooperate with Hst1 to achieve anti-inflammatory and anti-oxidative stress effects. Subsequent cell and animal experiments further confirmed that Hst1@PNPs have the effects of anti-inflammatory, scavenging ROS, promoting re-epithelialization, collagen deposition and angiogenesis, thereby promoting diabetic wound healing.

The invention belongs to the field of biological medicine, and discloses a preparation method and application of a mesenchymal stem cell-derived exosome entrapped valproic acid compound. According to the invention, the mesenchymal stem cell source exosome is used as a carrier, valproic acid is entrapped, and the compound of VPA entrapped by the exosome of MSCs is prepared by an ultrasonic method, so that the VPA-EXO prepared by the invention is proved to be capable of promoting faster repair of the wound surface by inhibiting inflammation, promoting collagen formation, accelerating angiogenesis and reepithelization of the wound surface. The valproic acid-loaded human umbilical cord mesenchymal stem cell exosome prepared by the invention is VPA-EXO prepared by an ultrasonic method, has no obvious toxicity to cells, and has obvious advantages.

According to the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the surface stem cells, the brand new pharmacological action of the dipsacus asper saponin VI in targeted promotion of the differentiation of the epidermal stem cells is found for the first time, the cognitive limitation of the prior art is broken through, a brand new target spot and direction are provided for research and development of innovative drugs for wound repair, and the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the epidermal stem cells is realized. A medicine containing teasel saponin VI is adopted, epidermal stem cells are taken as a core target, wound reepithelialization is accelerated by promoting directional differentiation of the epidermal stem cells, the mechanism is clear, the targeting property is high, a core link capable of directly hitting wound healing is adopted, epidermal stem cell differentiation is regulated in a targeted manner, and meanwhile, the medicine has the synergistic effects of resisting inflammation and promoting angiogenesis; the obvious application potential is realized on refractory wounds such as diabetic feet and pressure sores.

Disclosed is a composition (204) comprising galactose and fucose in monosaccharide form for use as a promotor of tissue re-epithelialization, wherein the tissue is non-ophthalmic tissue.

The present invention relates to the technical field of surgical wound infection monitoring, and in particular to a Pt-MOFs nanomotor and coated suture for surgical wound infection monitoring and a preparation method thereof. It is prepared by selective anisotropic growth of Cu-based tetra(4-pyridylphenyl)ethylene (TPPE) nanoscale metal organic framework Cu-MOFs on Pt nanodendrites; the Pt nanodendrites are used as propulsion elements, and AIE luminescent prototype MOFs are used as fluorescence generating elements and antibacterial agents. The surface of the suture is coated with the above-mentioned Pt-MOFs nanomotor. The present invention is made by coating a janus-type nanomotor on a medical suture, and this nanomotor can improve the permeability of bacterial biofilms by self-propulsion, thereby enhancing the interaction between bacterial cells and antibacterial drugs. The Pt-MOFs nanomotor exhibits excellent therapeutic ability in accelerating wound healing, such as reducing inflammatory infiltration, enhancing collagen deposition, improving re-epithelialization and increasing CD31 expression, providing a new strategy for monitoring and treating surgical wound infections in clinical practice.

The application provides a macrophage-targeted FGF19 sustained-release delivery system, a preparation method and an application. The system comprises FGF19-loaded macrophage membrane-derived nanoparticles and a temperature-sensitive hydrogel embedding the nanoparticles. The temperature-sensitive hydrogel is made of chitosan, poloxamer and beta-glycerophosphate sodium. The size of the macrophage membrane-derived nanoparticles is 300-800 nm. The system is an injectable liquid at 0-10 DEG C and forms a gel at 20-37 DEG C, achieving sustained release of FGF19 and drug-loaded vesicles. The system can promote re-epithelialization, collagen deposition, angiogenesis and M2 macrophage infiltration of a wound surface and is used for preparing a medicine or a dressing for promoting skin wound repair.

The invention belongs to the technical field of biological medicines, and particularly relates to application of human umbilical cord mesenchymal stem cells combined with metformin in preparation of anti-DFU medicines. By constructing a diabetic foot ulcer rat model and combining in-vivo and in-vitro experiments and signal analysis, it is confirmed for the first time that hUC-MSCs and metformin have a synergistic effect in treating diabetic foot ulcer. Combined treatment is beneficial to diabetic foot ulcer wound closure, reepithelization, matrix remodeling and proliferation of blood vessels and inflammation related cells, regulation and control of vascular endothelial cells PI3K-AKT and downstream signal channels thereof can be promoted through paracrine, so that the inhibited survival, proliferation, migration and vascularization capacities of the vascular endothelial cells PI3K-AKT in a hyperglycemia environment are recovered, and the vascular endothelial cells PI3K-AKT and the downstream signal channels of the vascular endothelial cells PI3K-AKT are promoted. Further, the dynamic regulation of enhancement, maturation and weakening of new angiogenesis of the wound of the DFU rat is promoted, and the wound healing is accelerated. The invention provides theoretical basis and experimental support for developing a more effective treatment scheme.

The invention discloses hydrogel as well as a preparation method and application thereof, belongs to the technical field of medical materials, and solves the problems of high recurrence rate and low curative effect of a treatment strategy of a diabetic wound in long-term treatment in the prior art. The method comprises the following steps: mixing a glutaminase inhibitor and a nano drug loading system, centrifuging, and collecting the precipitate to obtain a glutaminase inhibitor carrier, the drug loading system having oxidation resistance and drug loading capacity; mixing the modified hyaluronic acid with a gelatin solution to obtain gel; and adding the glutaminase inhibitor and the carrier into the gel, co-stirring, and adjusting the pH value to 7-8 to obtain the hydrogel. The hydrogel provided by the invention realizes specific removal of senescent cells from the source and remodeling of the ecological niche of the wound surface at the same time, so as to promote reepithelization and collagen deposition of diabetic tissues.

The application belongs to the technical field of biomedical materials and regenerative medicine, and provides a composite nano hydrogel and application thereof in repair of diabetic wound surface. The three-dimensional porous nano hydrogel framework formed by composite crosslinking of HA-3APBA, PVA and lithium clay nanosheet is used as a carrier, antioxidant small molecules NAC and VC are double-loaded, and inactivated umbilical cord serum is dispersed and compounded. In view of the pathological characteristics of high oxidative stress and sustained inflammation of the diabetic wound surface, the application removes active oxygen through the antioxidant small molecules, remodels the wound microenvironment, protects and cooperates with the umbilical cord serum to play the roles of promoting angiogenesis, collagen deposition and re-epithelialization, and solves the problems of easy inactivation of growth factors, prolonged inflammation and healing stagnation of the diabetic wound surface. Animal experiments show that the composite nano hydrogel significantly improves the healing speed and quality of the diabetic wound surface, and can be used for preparing drugs for treating diabetic foot ulcers, diabetic chronic ulcers and high oxidative stress difficult-to-heal wound surfaces.

The invention relates to the technical field of drugs for treating diabetic ulcer, in particular to application of genkwanin in preparation of drugs for treating diabetic ulcer. It is found that genkwanin has the effect of treating diabetic ulcer, a remarkable treatment effect is achieved, healing and reepithelization of diabetic ulcer wounds can be promoted, the content of active oxygen in the diabetic ulcer wounds can be reduced, and technical support is provided for clinically developing efficient strategies to treat diabetic ulcer in the future.

This invention discloses a near-infrared photoresponsive hydrogel, its preparation method, and its application in wound repair. The method includes the following steps: S1, modifying chitosan with 1,3-propanesulfonate lactone to prepare sulfonated chitosan; S2, mixing sulfonated chitosan solution, chitosan solution, genipin solution, and NaOH solution to prepare a hydrogel solution; S3, blending MXene, MOF, and the hydrogel solution to prepare a near-infrared photoresponsive hydrogel. The composite hydrogel provided by this invention utilizes the biomimetic structure and good biocompatibility of sulfonated chitosan, combined with the photothermal properties of MXene and the catalytic and ion-release characteristics of MOF, to achieve a dual function of broad-spectrum and highly effective antibacterial activity and multi-pathway promotion of skin tissue regeneration. This provides a novel integrated solution for the treatment of infected wounds, combining anti-infection, promotion of vascularization, and re-epithelialization.

The application discloses a double-layer microneedle patch and application thereof in preparation of a medicine for promoting wound healing of the elderly, and the double-layer microneedle patch comprises: an outer layer, which is hyaluronic acid (HA) loaded with lidocaine hydrochloride (LiH); and an inner layer, which is methacrylated gelatin (GelMA) loaded with hydroxytyrosol (HT). The wound healing process of the elderly mice is significantly accelerated, the wound area is small, the speed of natural exfoliation of the scab is faster, and the degree of re-epithelialization of the wound is higher, the collagen arrangement is more dense and ordered, and the regeneration of hair papilla cells in the wound area is increased. The double-layer microneedle patch has great application prospect in preparation of the medicine for promoting wound healing of the elderly.

The application provides a kind of genetic engineering exosome loaded with recombinant XVII type full-length human collagen and preparation and application, it is related to the field of bioengineering technology.The genetic engineering exosome provided by the application retains the intracellular domain, transmembrane domain, extracellular domain of XVII collagen completely, and also increases the exosome sorting signal peptide.The application also provides an extraction and separation method of the genetic engineering exosome loaded with recombinant XVII full-length human collagen and application in burn, scald, alopecia, oral ulcer and skin photoaging.The exosome prepared by the application is highly enriched with recombinant XVII type full-length human collagen on the exosome membrane, and retains the physiological activity of XVII type collagen, promotes the proliferation of epidermal cells, fibroblasts and hair follicle stem cells, plays the role of promoting re-epithelialization, promotes wound healing, promotes hair regeneration and delays skin aging.

This invention provides a reactive oxygen species (ROS)-responsive hydrogen sulfide donor, an ROS-responsive hydrogen sulfide-controlled release cationic chitosan sponge, its preparation method, and its applications, belonging to the field of medical materials. The ROS-responsive hydrogen sulfide-controlled release cationic chitosan sponge, prepared from quaternized chitosan and an ROS-responsive hydrogen sulfide donor, can controllably and on-demand release hydrogen sulfide under ROS stimulation. It can both reduce intracellular ROS levels and increase hydrogen sulfide levels. By inhibiting NF-κB activation, it regulates macrophage polarization towards the M2 phenotype, reduces the expression of inflammatory factors and eliminates excessive inflammation, enhances the migration and angiogenesis of human umbilical vein endothelial cells, and accelerates diabetic wound healing by promoting re-epithelialization, collagen deposition, and angiogenesis while simultaneously reducing inflammatory responses. The prepared ROS-responsive hydrogen sulfide-controlled release cationic chitosan sponge provides a promising solution for diabetic wound repair.

The invention belongs to the technical field of medicines, and particularly relates to an sECM-SIS biological membrane as well as a preparation method and application thereof. According to the present invention, an extracellular matrix (sECM) derived from stem cells is integrated to a small intestine submucosa (SIS) to prepare the sECM-SIS biological membrane; after the sECM-SIS biological membrane is implanted into a full-thickness skin defect, wound healing is remarkably accelerated, and the sECM-SIS biological membrane is characterized by rapid wound reepithelization, strong angiogenesis, approximate normal epidermis thickness recovery, mature collagen deposition, skin appendage regeneration and macrophage polarization adjustment. According to the results, the sECM-SIS has huge potential in the aspect of skin wound repair.

This invention relates to the field of biomimetic nanozymes and their preparation technology, disclosing a biomimetic nanozyme, its preparation method, and its applications. A peptide-functionalized biomimetic nanozyme (HRPL) is obtained by loading rapamycin onto HMPB nanozymes, coating the surface with a platelet membrane, and functionalizing it with the integrin-binding peptide LXW7. The binding of the platelet membrane to the LXW7 peptide enables targeted delivery to the site of endothelial injury, promoting endothelial repair and re-epithelialization. In an acidic inflammatory microenvironment, HRPL releases rapamycin, inhibiting smooth muscle cell proliferation and migration, and preventing restenosis. HRPL also scavenges reactive oxygen species, reducing oxidative stress and local inflammation, thereby improving the vascular microenvironment. The therapeutic effect was evaluated using a typical rat carotid balloon injury model. This dual-targeting mechanism not only promotes endothelial repair but also reduces restenosis and inflammation, showing significant clinical therapeutic potential. Especially after stent implantation, HRPL helps improve prognosis, reduce complications, and restore vascular homeostasis.

The invention provides a lithium chloride-PDLLA-PEG-PDLLA hydrogel compound solution and a preparation method thereof.The preparation method comprises the steps that polyethylene glycol PEG and D and L-lactide D and L-LA are synthesized to prepare a PDLLA-PEG-PDLLA (PLEL) block copolymer with temperature-sensitive phase change characteristics and biocompatibility, then the PDLLA-PEG-PDLLA (PLEL) block copolymer is dissolved in a proper amount of water, finally, lithium chloride is added, the mixture is mixed to be uniform, and the hydrogel compound solution is obtained; the hydrogel compound is in a liquid state at room temperature, can be easily delivered in vivo in an injection manner, then is injected to a target part, and is rapidly converted into a gel state in an environment close to body temperature (about 37 DEG C) to form a stable hydrogel compound. The hydrogel compound solution prepared by the invention can form an effective protective barrier on a wound surface, can promote re-epithelization of the wound surface through accurate drug release and cell behavior regulation and control, can significantly improve the overall repair effect of the skin wound surface, reduces the formation of scars, and improves the life quality of a patient.

Provided are a composition and a sheet, including a mesenchymal stem cells-hydrogel-biodegradable support or a mesenchymal stem cells-hydrogel-nondegradable support and a preparing method thereof. More specifically, in the sheet including a mesenchymal stem cells-hydrogel-biodegradable support or a mesenchymal stem cells-hydrogel-nondegradable support according to the present invention, the high-active mesenchymal stem cells may be applied to a wounded part of a patient with epidermolysis bullosa as it is without isolation using proteases, and in the culturing, an extracellular matrix such as collagen, laminin, fibronectin, and elastin secreted from the mesenchymal stem cells is wholly present on the hydrogel to have an advantageous effect that skin reproduction and re-epithelization abilities are significantly excellent as compared with conventional dressing agents used for epidermolysis bullosa.

The application provides a preparation method of giant salamander skin in-situ collagen hydrogel dressing and application thereof in promoting burn wound healing, and belongs to the technical field of wound repair dressing. The in-situ collagen hydrogel dressing prepared from 1-3-year-old giant salamander skin through pretreatment, decolorization, removal of impure protein, degreasing and other steps has a natural three-dimensional honeycomb porous structure. The water vapor transmission rate of the dressing is 2000-2500 g / (m 2 24h), the dressing has excellent water retention and dynamic fitting properties, can realize efficient drug loading and slow release, has a significant bacteriostatic effect on escherichia coli and staphylococcus aureus, and has no cytotoxicity. Animal experiments show that the dressing can effectively reduce wound inflammation, promote granulation tissue growth and re-epithelialization, and significantly improve the healing speed and quality of deep Ⅱ degree burn wounds and other wounds. The application has simple process, good biocompatibility, good mechanical and functional adaptability, and is suitable for the repair and nursing of infected wounds and burn wounds, and has good clinical transformation and application prospect.

The invention belongs to the technical field of biological materials, and particularly relates to platelet-rich plasma exosome hydrogel as well as a preparation method and application thereof. According to the hydrogel containing the platelet-rich plasma exosome, the platelet-rich plasma exosome is dispersed in modified ethylene glycol chitosan hydrogel, and the mass ratio of the platelet-rich plasma exosome to the modified ethylene glycol chitosan hydrogel is 1: (1000-2000). The CBGCTS hydrogel disclosed by the invention is used for repairing skin wounds, is beneficial to wound healing, can be used as a support of PRP-exo exosomes, and can overcome the defects caused by pure application of the exosomes. The CBGCTS (at) EXO hydrogel obtained by combining the exosome and the CBGCTS gel can be used for effectively accelerating skin wound healing by promoting reepithelialization, deposition and remodeling of an STZ-induced diabetic wound of a diabetic rat model, enhancing angiogenesis and neuronal inward growth and reducing inflammation, so that the healing of the skin wound is accelerated. Therefore, a novel non-invasive application method of the exosome with practical value is provided for skin repair.

The application discloses a double-sided hydrogel scaffold for urethral repair and a preparation process thereof, and belongs to the technical field of biomaterials. The PCH@PVA scaffold is constructed, a base layer on the tissue side creates a eutrophication and guiding microenvironment for regeneration by using heparin, and a functional layer on the lumen side effectively blocks the penetration of harmful components in urine and the colonization of bacteria by a polyvinyl alcohol coating, and by optimizing the crosslinking degree of PVA, the PVA has excellent mechanical toughness and elasticity, can not only withstand the periodic expansion of the urethra without breaking, but also can transmit mechanical stimulation close to the physiological range, guide cells to functional regeneration instead of pathological fibrosis. The PCH@PVA scaffold provided by the application has excellent effects in promoting re-epithelialization, angiogenesis, inhibiting fibrosis and preventing stenosis. The application also provides a new design paradigm and theoretical basis for tissue regeneration strategies in other complex physiological microenvironments.

The invention provides an active oxygen responsive hydrogen sulfide donor, an active oxygen responsive hydrogen sulfide controlled release type cationized chitosan sponge as well as a preparation method and application thereof, and belongs to the field of medical materials. The active oxygen responsive hydrogen sulfide controlled release type cationized chitosan sponge prepared from quaternized chitosan and an active oxygen responsive hydrogen sulfide donor can controllably release hydrogen sulfide as required under the stimulation of active oxygen, can reduce the level of active oxygen in cells, can improve the level of hydrogen sulfide, and can inhibit the activation of NF-kappa B so as to improve the activity of hydrogen sulfide. The traditional Chinese medicine composition can be used for regulating macrophage phenotype polarization to M2, reducing cell inflammatory factor expression, eliminating excessive inflammation, enhancing migration and angiogenesis capability of human umbilical vein endothelial cells, promoting reepithelization, collagen deposition and angiogenesis, relieving inflammatory response and accelerating healing of diabetic wounds at the same time. The prepared active oxygen response hydrogen sulfide controlled release type cationized chitosan sponge provides a promising solution for diabetic wound repair.

The application relates to a biological polysaccharide dressing for promoting wound repair and a preparation method, which comprises the following components in percentage by mass volume: 2.5-3.0% of sodium carboxymethyl cellulose, 0.6-0.9% of sodium chloride, 0.2-0.3% of anhydrous sodium acetate, 0.3-0.5% of sodium gluconate, 0.02-0.03% of magnesium chloride, 0.02-0.04% of potassium chloride, 0.14-0.18% of extract of Radix Dendrobii, 0.08-0.12% of extract of Marchantia, 0.04-0.08% of extract of Gekko, 0.03-0.05% of inactivated Lactobacillus, and the balance of purified water. The dressing can promote the migration of keratinocytes, accelerate re-epithelialization of a wound, and relieve scar itching through the synergistic effect of the polysaccharide matrix and multiple components, and is suitable for postoperative non-chronic wound care.

The invention discloses a drug-release nanoparticle reinforced hydrogel and a preparation method and application thereof, the drug-release nanoparticle reinforced hydrogel comprises: drug nanoparticles including active drug components wrapped by phospholipid-acrylate polymer; the porous hydrogel is formed by crosslinking an acrylated biodegradable polymer and a pore-foaming agent, the porous hydrogel is loaded with the drug nanoparticles. The drug-release nanoparticle-reinforced hydrogel can realize more than 90% of drug release, can adjust within 4-72 hours, and significantly improves the wound healing rate and skin regeneration. The hydrogel has biocompatibility, can promote reepithelization, angiogenesis and collagen regeneration, and leads to minimal scar formation.

A method of treating human or animal tissue, the method comprising the step of: i. directing a stream of an aqueous liquid comprising gas bubbles excited by acoustic energy towards a wound or anatomical pocket in human or animal tissue, or towards an anatomical space or potential space in human or animal tissue, or towards soft or hard tissue in an oral cavity or elsewhere in the human or animal body, or towards tissue in a nasal cavity, or towards tissue associated with sinuses, eye, ear, digestive and genito-urinary systems, thereby to treat the human or animal tissue with the stream. The output stream can clean a wound in human or animal tissue, and can treat the wound by healing the wound, for example by stimulating fibroblasts in the wound tissue and causing, promoting or enhancing re-epithelialisation of epidermal tissue in the wound.

The present invention relates to Lactobacillus plantarum strain NCIMB 43029; Lactobacillus acidophilus strain NCIMB 43030; a composition having antibacterial and re-epithelialization effects, the composition comprising Lactobacillus plantarum and Lactobacillus acidophilus and optionally comprising Streptococcus thermophilus and / or Bacillus amyloliquefaciens, for use as a medicament for re-epithelialization of an injury or wound; a bandage comprising the composition and a kit comprising such a composition.

The invention discloses a preparation method and application of a double-disc structural tracheal fistula patch. The method comprises the following steps: constructing a silk fibroin (SF)-based double-disk structure embedded repair patch (CRP) with a physical and chemical double-crosslinking network; and by utilizing the ultra-light weight, good elasticity and water absorption, the double-sided disc-shaped structure can be embedded into a defect repair position, so that the effect of tight repair is achieved. The tracheal fistula can be stably repaired in situ, complications such as low success rate, stent displacement fracture, insufficient fistula orifice plugging and new biological hyperplasia in a lumen caused by a traditional stent are avoided, re-epithelization of a defect part is effectively promoted, the stent is expected to become an ideal stent for treating the tracheal fistula, and a new hope is brought to treatment of the tracheal fistula.

The invention discloses a double-layer microneedle patch and application thereof in preparation of a medicine for promoting wound healing of old people. The double-layer microneedle patch comprises an outer layer, an inner layer and an outer layer, wherein the outer layer is hyaluronic acid (HA) loaded lidocaine hydrochloride (LiH); and the inner layer is prepared by loading hydroxytyrosol (HT) on methacrylated gelatin (GelMA). The wound healing process of the old mice is remarkably accelerated, the wound area is small, the natural crust falling speed is higher, the wound reepithelization degree is higher, collagen arrangement is more compact and ordered, and hair papilla cell regeneration in the wound area is increased. The double-layer microneedle patch has a great application prospect in preparation of medicines for promoting wound healing of old people.