10 results about "Re-epithelialization" patented technology

According to the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the surface stem cells, the brand new pharmacological action of the dipsacus asper saponin VI in targeted promotion of the differentiation of the epidermal stem cells is found for the first time, the cognitive limitation of the prior art is broken through, a brand new target spot and direction are provided for research and development of innovative drugs for wound repair, and the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the epidermal stem cells is realized. A medicine containing teasel saponin VI is adopted, epidermal stem cells are taken as a core target, wound reepithelialization is accelerated by promoting directional differentiation of the epidermal stem cells, the mechanism is clear, the targeting property is high, a core link capable of directly hitting wound healing is adopted, epidermal stem cell differentiation is regulated in a targeted manner, and meanwhile, the medicine has the synergistic effects of resisting inflammation and promoting angiogenesis; the obvious application potential is realized on refractory wounds such as diabetic feet and pressure sores.

The application belongs to the technical field of biomedical materials and regenerative medicine, and provides a composite nano hydrogel and application thereof in repair of diabetic wound surface. The three-dimensional porous nano hydrogel framework formed by composite crosslinking of HA-3APBA, PVA and lithium clay nanosheet is used as a carrier, antioxidant small molecules NAC and VC are double-loaded, and inactivated umbilical cord serum is dispersed and compounded. In view of the pathological characteristics of high oxidative stress and sustained inflammation of the diabetic wound surface, the application removes active oxygen through the antioxidant small molecules, remodels the wound microenvironment, protects and cooperates with the umbilical cord serum to play the roles of promoting angiogenesis, collagen deposition and re-epithelialization, and solves the problems of easy inactivation of growth factors, prolonged inflammation and healing stagnation of the diabetic wound surface. Animal experiments show that the composite nano hydrogel significantly improves the healing speed and quality of the diabetic wound surface, and can be used for preparing drugs for treating diabetic foot ulcers, diabetic chronic ulcers and high oxidative stress difficult-to-heal wound surfaces.

This invention discloses a near-infrared photoresponsive hydrogel, its preparation method, and its application in wound repair. The method includes the following steps: S1, modifying chitosan with 1,3-propanesulfonate lactone to prepare sulfonated chitosan; S2, mixing sulfonated chitosan solution, chitosan solution, genipin solution, and NaOH solution to prepare a hydrogel solution; S3, blending MXene, MOF, and the hydrogel solution to prepare a near-infrared photoresponsive hydrogel. The composite hydrogel provided by this invention utilizes the biomimetic structure and good biocompatibility of sulfonated chitosan, combined with the photothermal properties of MXene and the catalytic and ion-release characteristics of MOF, to achieve a dual function of broad-spectrum and highly effective antibacterial activity and multi-pathway promotion of skin tissue regeneration. This provides a novel integrated solution for the treatment of infected wounds, combining anti-infection, promotion of vascularization, and re-epithelialization.

The application provides a kind of genetic engineering exosome loaded with recombinant XVII type full-length human collagen and preparation and application, it is related to the field of bioengineering technology.The genetic engineering exosome provided by the application retains the intracellular domain, transmembrane domain, extracellular domain of XVII collagen completely, and also increases the exosome sorting signal peptide.The application also provides an extraction and separation method of the genetic engineering exosome loaded with recombinant XVII full-length human collagen and application in burn, scald, alopecia, oral ulcer and skin photoaging.The exosome prepared by the application is highly enriched with recombinant XVII type full-length human collagen on the exosome membrane, and retains the physiological activity of XVII type collagen, promotes the proliferation of epidermal cells, fibroblasts and hair follicle stem cells, plays the role of promoting re-epithelialization, promotes wound healing, promotes hair regeneration and delays skin aging.

This invention provides a reactive oxygen species (ROS)-responsive hydrogen sulfide donor, an ROS-responsive hydrogen sulfide-controlled release cationic chitosan sponge, its preparation method, and its applications, belonging to the field of medical materials. The ROS-responsive hydrogen sulfide-controlled release cationic chitosan sponge, prepared from quaternized chitosan and an ROS-responsive hydrogen sulfide donor, can controllably and on-demand release hydrogen sulfide under ROS stimulation. It can both reduce intracellular ROS levels and increase hydrogen sulfide levels. By inhibiting NF-κB activation, it regulates macrophage polarization towards the M2 phenotype, reduces the expression of inflammatory factors and eliminates excessive inflammation, enhances the migration and angiogenesis of human umbilical vein endothelial cells, and accelerates diabetic wound healing by promoting re-epithelialization, collagen deposition, and angiogenesis while simultaneously reducing inflammatory responses. The prepared ROS-responsive hydrogen sulfide-controlled release cationic chitosan sponge provides a promising solution for diabetic wound repair.

This invention relates to the field of biomimetic nanozymes and their preparation technology, disclosing a biomimetic nanozyme, its preparation method, and its applications. A peptide-functionalized biomimetic nanozyme (HRPL) is obtained by loading rapamycin onto HMPB nanozymes, coating the surface with a platelet membrane, and functionalizing it with the integrin-binding peptide LXW7. The binding of the platelet membrane to the LXW7 peptide enables targeted delivery to the site of endothelial injury, promoting endothelial repair and re-epithelialization. In an acidic inflammatory microenvironment, HRPL releases rapamycin, inhibiting smooth muscle cell proliferation and migration, and preventing restenosis. HRPL also scavenges reactive oxygen species, reducing oxidative stress and local inflammation, thereby improving the vascular microenvironment. The therapeutic effect was evaluated using a typical rat carotid balloon injury model. This dual-targeting mechanism not only promotes endothelial repair but also reduces restenosis and inflammation, showing significant clinical therapeutic potential. Especially after stent implantation, HRPL helps improve prognosis, reduce complications, and restore vascular homeostasis.

The application discloses a double-sided hydrogel scaffold for urethral repair and a preparation process thereof, and belongs to the technical field of biomaterials. The PCH@PVA scaffold is constructed, a base layer on the tissue side creates a eutrophication and guiding microenvironment for regeneration by using heparin, and a functional layer on the lumen side effectively blocks the penetration of harmful components in urine and the colonization of bacteria by a polyvinyl alcohol coating, and by optimizing the crosslinking degree of PVA, the PVA has excellent mechanical toughness and elasticity, can not only withstand the periodic expansion of the urethra without breaking, but also can transmit mechanical stimulation close to the physiological range, guide cells to functional regeneration instead of pathological fibrosis. The PCH@PVA scaffold provided by the application has excellent effects in promoting re-epithelialization, angiogenesis, inhibiting fibrosis and preventing stenosis. The application also provides a new design paradigm and theoretical basis for tissue regeneration strategies in other complex physiological microenvironments.

The invention belongs to the technical field of biological medicines, and discloses a hydrogel antibacterial dressing containing dragon's blood oil and a preparation method thereof. The hydrogel is characterized by comprising a three-dimensional hydrogel matrix formed by co-crosslinking methylacryloylated hyaluronic acid and gelatin, an in-situ compounded PEDOT: PSS / sodium carboxymethyl cellulose conductive nanofiber network, and dragon's blood oil microcapsules which are self-assembled and coated layer by layer by sodium alginate-chitosan and are modified by PEG (Polyethylene Glycol). Through the design, the dressing has stable conductivity (1.2-2.5 S / m), dual-phase drug release and excellent biocompatibility, synergistically promotes cell migration and microenvironment regulation and control under a low-intensity direct-current electric field, and significantly accelerates reepithelization and revascularization of diabetic wounds. According to the invention, the limitation of a dressing with a single function is overcome, the space-time coupling of a physical stimulation signal and a biochemical regulation signal is realized on a material architecture level, and an efficient, safe and clinically convertible novel treatment scheme is provided for the refractory wound of diabetes mellitus.

The invention relates to a wound repair material, in particular to a three-dimensional printing composite hydrogel with antibacterial and immune regulation functions as well as a preparation method and application of the three-dimensional printing composite hydrogel. The three-dimensional printing composite hydrogel comprises a three-dimensional cross-linked network skeleton formed by gelatin methylacrylate and hyaluronic acid methylacrylate, the mixed charge network structure is formed by methacryloyloxyethyl trimethyl ammonium chloride and itaconic acid and contains cations and carboxyl; wherein the hybrid charge network structure is fixed in the three-dimensional cross-linked network skeleton through covalent bonds. Compared with the prior art, the problems that existing hydrogel is single in function and lacks internal antibacterial and inflammation regulation capacity, and printability is poor due to insufficient rheological property are solved. According to the scheme, through the structure-function integrated design, the material can have multiple internal functions of contact antibiosis, inflammatory reaction relief, angiogenesis promotion and the like under the condition that exogenous drugs are not needed, and wound reepithelization acceleration and tissue repair and collagen reconstruction promotion are facilitated.