24 results about "Re-epithelialization" patented technology

The invention discloses a photo-thermal composite hydrogel patch and a preparation method and application thereof.The hydrogel patch is of a three-dimensional latticed porous structure formed by taking methylacryloyl gelatin (GelMA) and methylacryloyl hyaluronic acid (HAMA) as matrixes, loading niobium carbide nano-particles (Nb2CNPs) and vinipofen (VP) and conducting 3D printing or casting molding, the hydrogel patch is named as VP-Nb2C-coated GelMA / HAMA (VNGH) hydrogel patch for short. The VNGH has good photo-thermal antibacterial property, oxidation resistance and reepithelization promoting function, can significantly improve the quality of mouse diabetic wound healing, and provides a new thought and strategy for realizing scar-free healing of diabetic wounds.

According to the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the surface stem cells, the brand new pharmacological action of the dipsacus asper saponin VI in targeted promotion of the differentiation of the epidermal stem cells is found for the first time, the cognitive limitation of the prior art is broken through, a brand new target spot and direction are provided for research and development of innovative drugs for wound repair, and the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the epidermal stem cells is realized. A medicine containing teasel saponin VI is adopted, epidermal stem cells are taken as a core target, wound reepithelialization is accelerated by promoting directional differentiation of the epidermal stem cells, the mechanism is clear, the targeting property is high, a core link capable of directly hitting wound healing is adopted, epidermal stem cell differentiation is regulated in a targeted manner, and meanwhile, the medicine has the synergistic effects of resisting inflammation and promoting angiogenesis; the obvious application potential is realized on refractory wounds such as diabetic feet and pressure sores.

Disclosed is a composition (204) comprising galactose and fucose in monosaccharide form for use as a promotor of tissue re-epithelialization, wherein the tissue is non-ophthalmic tissue.

The application provides a macrophage-targeted FGF19 sustained-release delivery system, a preparation method and an application. The system comprises FGF19-loaded macrophage membrane-derived nanoparticles and a temperature-sensitive hydrogel embedding the nanoparticles. The temperature-sensitive hydrogel is made of chitosan, poloxamer and beta-glycerophosphate sodium. The size of the macrophage membrane-derived nanoparticles is 300-800 nm. The system is an injectable liquid at 0-10 DEG C and forms a gel at 20-37 DEG C, achieving sustained release of FGF19 and drug-loaded vesicles. The system can promote re-epithelialization, collagen deposition, angiogenesis and M2 macrophage infiltration of a wound surface and is used for preparing a medicine or a dressing for promoting skin wound repair.

The invention belongs to the technical field of biological medicines, and particularly relates to application of human umbilical cord mesenchymal stem cells combined with metformin in preparation of anti-DFU medicines. By constructing a diabetic foot ulcer rat model and combining in-vivo and in-vitro experiments and signal analysis, it is confirmed for the first time that hUC-MSCs and metformin have a synergistic effect in treating diabetic foot ulcer. Combined treatment is beneficial to diabetic foot ulcer wound closure, reepithelization, matrix remodeling and proliferation of blood vessels and inflammation related cells, regulation and control of vascular endothelial cells PI3K-AKT and downstream signal channels thereof can be promoted through paracrine, so that the inhibited survival, proliferation, migration and vascularization capacities of the vascular endothelial cells PI3K-AKT in a hyperglycemia environment are recovered, and the vascular endothelial cells PI3K-AKT and the downstream signal channels of the vascular endothelial cells PI3K-AKT are promoted. Further, the dynamic regulation of enhancement, maturation and weakening of new angiogenesis of the wound of the DFU rat is promoted, and the wound healing is accelerated. The invention provides theoretical basis and experimental support for developing a more effective treatment scheme.

The application belongs to the technical field of biomedical materials and regenerative medicine, and provides a composite nano hydrogel and application thereof in repair of diabetic wound surface. The three-dimensional porous nano hydrogel framework formed by composite crosslinking of HA-3APBA, PVA and lithium clay nanosheet is used as a carrier, antioxidant small molecules NAC and VC are double-loaded, and inactivated umbilical cord serum is dispersed and compounded. In view of the pathological characteristics of high oxidative stress and sustained inflammation of the diabetic wound surface, the application removes active oxygen through the antioxidant small molecules, remodels the wound microenvironment, protects and cooperates with the umbilical cord serum to play the roles of promoting angiogenesis, collagen deposition and re-epithelialization, and solves the problems of easy inactivation of growth factors, prolonged inflammation and healing stagnation of the diabetic wound surface. Animal experiments show that the composite nano hydrogel significantly improves the healing speed and quality of the diabetic wound surface, and can be used for preparing drugs for treating diabetic foot ulcers, diabetic chronic ulcers and high oxidative stress difficult-to-heal wound surfaces.

This invention discloses a near-infrared photoresponsive hydrogel, its preparation method, and its application in wound repair. The method includes the following steps: S1, modifying chitosan with 1,3-propanesulfonate lactone to prepare sulfonated chitosan; S2, mixing sulfonated chitosan solution, chitosan solution, genipin solution, and NaOH solution to prepare a hydrogel solution; S3, blending MXene, MOF, and the hydrogel solution to prepare a near-infrared photoresponsive hydrogel. The composite hydrogel provided by this invention utilizes the biomimetic structure and good biocompatibility of sulfonated chitosan, combined with the photothermal properties of MXene and the catalytic and ion-release characteristics of MOF, to achieve a dual function of broad-spectrum and highly effective antibacterial activity and multi-pathway promotion of skin tissue regeneration. This provides a novel integrated solution for the treatment of infected wounds, combining anti-infection, promotion of vascularization, and re-epithelialization.

The application discloses a double-layer microneedle patch and application thereof in preparation of a medicine for promoting wound healing of the elderly, and the double-layer microneedle patch comprises: an outer layer, which is hyaluronic acid (HA) loaded with lidocaine hydrochloride (LiH); and an inner layer, which is methacrylated gelatin (GelMA) loaded with hydroxytyrosol (HT). The wound healing process of the elderly mice is significantly accelerated, the wound area is small, the speed of natural exfoliation of the scab is faster, and the degree of re-epithelialization of the wound is higher, the collagen arrangement is more dense and ordered, and the regeneration of hair papilla cells in the wound area is increased. The double-layer microneedle patch has great application prospect in preparation of the medicine for promoting wound healing of the elderly.

The application provides a kind of genetic engineering exosome loaded with recombinant XVII type full-length human collagen and preparation and application, it is related to the field of bioengineering technology.The genetic engineering exosome provided by the application retains the intracellular domain, transmembrane domain, extracellular domain of XVII collagen completely, and also increases the exosome sorting signal peptide.The application also provides an extraction and separation method of the genetic engineering exosome loaded with recombinant XVII full-length human collagen and application in burn, scald, alopecia, oral ulcer and skin photoaging.The exosome prepared by the application is highly enriched with recombinant XVII type full-length human collagen on the exosome membrane, and retains the physiological activity of XVII type collagen, promotes the proliferation of epidermal cells, fibroblasts and hair follicle stem cells, plays the role of promoting re-epithelialization, promotes wound healing, promotes hair regeneration and delays skin aging.

This invention provides a reactive oxygen species (ROS)-responsive hydrogen sulfide donor, an ROS-responsive hydrogen sulfide-controlled release cationic chitosan sponge, its preparation method, and its applications, belonging to the field of medical materials. The ROS-responsive hydrogen sulfide-controlled release cationic chitosan sponge, prepared from quaternized chitosan and an ROS-responsive hydrogen sulfide donor, can controllably and on-demand release hydrogen sulfide under ROS stimulation. It can both reduce intracellular ROS levels and increase hydrogen sulfide levels. By inhibiting NF-κB activation, it regulates macrophage polarization towards the M2 phenotype, reduces the expression of inflammatory factors and eliminates excessive inflammation, enhances the migration and angiogenesis of human umbilical vein endothelial cells, and accelerates diabetic wound healing by promoting re-epithelialization, collagen deposition, and angiogenesis while simultaneously reducing inflammatory responses. The prepared ROS-responsive hydrogen sulfide-controlled release cationic chitosan sponge provides a promising solution for diabetic wound repair.

The invention belongs to the technical field of medicines, and particularly relates to an sECM-SIS biological membrane as well as a preparation method and application thereof. According to the present invention, an extracellular matrix (sECM) derived from stem cells is integrated to a small intestine submucosa (SIS) to prepare the sECM-SIS biological membrane; after the sECM-SIS biological membrane is implanted into a full-thickness skin defect, wound healing is remarkably accelerated, and the sECM-SIS biological membrane is characterized by rapid wound reepithelization, strong angiogenesis, approximate normal epidermis thickness recovery, mature collagen deposition, skin appendage regeneration and macrophage polarization adjustment. According to the results, the sECM-SIS has huge potential in the aspect of skin wound repair.

This invention relates to the field of biomimetic nanozymes and their preparation technology, disclosing a biomimetic nanozyme, its preparation method, and its applications. A peptide-functionalized biomimetic nanozyme (HRPL) is obtained by loading rapamycin onto HMPB nanozymes, coating the surface with a platelet membrane, and functionalizing it with the integrin-binding peptide LXW7. The binding of the platelet membrane to the LXW7 peptide enables targeted delivery to the site of endothelial injury, promoting endothelial repair and re-epithelialization. In an acidic inflammatory microenvironment, HRPL releases rapamycin, inhibiting smooth muscle cell proliferation and migration, and preventing restenosis. HRPL also scavenges reactive oxygen species, reducing oxidative stress and local inflammation, thereby improving the vascular microenvironment. The therapeutic effect was evaluated using a typical rat carotid balloon injury model. This dual-targeting mechanism not only promotes endothelial repair but also reduces restenosis and inflammation, showing significant clinical therapeutic potential. Especially after stent implantation, HRPL helps improve prognosis, reduce complications, and restore vascular homeostasis.

The invention provides a lithium chloride-PDLLA-PEG-PDLLA hydrogel compound solution and a preparation method thereof.The preparation method comprises the steps that polyethylene glycol PEG and D and L-lactide D and L-LA are synthesized to prepare a PDLLA-PEG-PDLLA (PLEL) block copolymer with temperature-sensitive phase change characteristics and biocompatibility, then the PDLLA-PEG-PDLLA (PLEL) block copolymer is dissolved in a proper amount of water, finally, lithium chloride is added, the mixture is mixed to be uniform, and the hydrogel compound solution is obtained; the hydrogel compound is in a liquid state at room temperature, can be easily delivered in vivo in an injection manner, then is injected to a target part, and is rapidly converted into a gel state in an environment close to body temperature (about 37 DEG C) to form a stable hydrogel compound. The hydrogel compound solution prepared by the invention can form an effective protective barrier on a wound surface, can promote re-epithelization of the wound surface through accurate drug release and cell behavior regulation and control, can significantly improve the overall repair effect of the skin wound surface, reduces the formation of scars, and improves the life quality of a patient.

The application provides a preparation method of giant salamander skin in-situ collagen hydrogel dressing and application thereof in promoting burn wound healing, and belongs to the technical field of wound repair dressing. The in-situ collagen hydrogel dressing prepared from 1-3-year-old giant salamander skin through pretreatment, decolorization, removal of impure protein, degreasing and other steps has a natural three-dimensional honeycomb porous structure. The water vapor transmission rate of the dressing is 2000-2500 g / (m 2 24h), the dressing has excellent water retention and dynamic fitting properties, can realize efficient drug loading and slow release, has a significant bacteriostatic effect on escherichia coli and staphylococcus aureus, and has no cytotoxicity. Animal experiments show that the dressing can effectively reduce wound inflammation, promote granulation tissue growth and re-epithelialization, and significantly improve the healing speed and quality of deep Ⅱ degree burn wounds and other wounds. The application has simple process, good biocompatibility, good mechanical and functional adaptability, and is suitable for the repair and nursing of infected wounds and burn wounds, and has good clinical transformation and application prospect.

The application discloses a double-sided hydrogel scaffold for urethral repair and a preparation process thereof, and belongs to the technical field of biomaterials. The PCH@PVA scaffold is constructed, a base layer on the tissue side creates a eutrophication and guiding microenvironment for regeneration by using heparin, and a functional layer on the lumen side effectively blocks the penetration of harmful components in urine and the colonization of bacteria by a polyvinyl alcohol coating, and by optimizing the crosslinking degree of PVA, the PVA has excellent mechanical toughness and elasticity, can not only withstand the periodic expansion of the urethra without breaking, but also can transmit mechanical stimulation close to the physiological range, guide cells to functional regeneration instead of pathological fibrosis. The PCH@PVA scaffold provided by the application has excellent effects in promoting re-epithelialization, angiogenesis, inhibiting fibrosis and preventing stenosis. The application also provides a new design paradigm and theoretical basis for tissue regeneration strategies in other complex physiological microenvironments.

The invention provides an active oxygen responsive hydrogen sulfide donor, an active oxygen responsive hydrogen sulfide controlled release type cationized chitosan sponge as well as a preparation method and application thereof, and belongs to the field of medical materials. The active oxygen responsive hydrogen sulfide controlled release type cationized chitosan sponge prepared from quaternized chitosan and an active oxygen responsive hydrogen sulfide donor can controllably release hydrogen sulfide as required under the stimulation of active oxygen, can reduce the level of active oxygen in cells, can improve the level of hydrogen sulfide, and can inhibit the activation of NF-kappa B so as to improve the activity of hydrogen sulfide. The traditional Chinese medicine composition can be used for regulating macrophage phenotype polarization to M2, reducing cell inflammatory factor expression, eliminating excessive inflammation, enhancing migration and angiogenesis capability of human umbilical vein endothelial cells, promoting reepithelization, collagen deposition and angiogenesis, relieving inflammatory response and accelerating healing of diabetic wounds at the same time. The prepared active oxygen response hydrogen sulfide controlled release type cationized chitosan sponge provides a promising solution for diabetic wound repair.

The application relates to a biological polysaccharide dressing for promoting wound repair and a preparation method, which comprises the following components in percentage by mass volume: 2.5-3.0% of sodium carboxymethyl cellulose, 0.6-0.9% of sodium chloride, 0.2-0.3% of anhydrous sodium acetate, 0.3-0.5% of sodium gluconate, 0.02-0.03% of magnesium chloride, 0.02-0.04% of potassium chloride, 0.14-0.18% of extract of Radix Dendrobii, 0.08-0.12% of extract of Marchantia, 0.04-0.08% of extract of Gekko, 0.03-0.05% of inactivated Lactobacillus, and the balance of purified water. The dressing can promote the migration of keratinocytes, accelerate re-epithelialization of a wound, and relieve scar itching through the synergistic effect of the polysaccharide matrix and multiple components, and is suitable for postoperative non-chronic wound care.

A method of treating human or animal tissue, the method comprising the step of: i. directing a stream of an aqueous liquid comprising gas bubbles excited by acoustic energy towards a wound or anatomical pocket in human or animal tissue, or towards an anatomical space or potential space in human or animal tissue, or towards soft or hard tissue in an oral cavity or elsewhere in the human or animal body, or towards tissue in a nasal cavity, or towards tissue associated with sinuses, eye, ear, digestive and genito-urinary systems, thereby to treat the human or animal tissue with the stream. The output stream can clean a wound in human or animal tissue, and can treat the wound by healing the wound, for example by stimulating fibroblasts in the wound tissue and causing, promoting or enhancing re-epithelialisation of epidermal tissue in the wound.

The invention discloses a double-layer microneedle patch and application thereof in preparation of a medicine for promoting wound healing of old people. The double-layer microneedle patch comprises an outer layer, an inner layer and an outer layer, wherein the outer layer is hyaluronic acid (HA) loaded lidocaine hydrochloride (LiH); and the inner layer is prepared by loading hydroxytyrosol (HT) on methacrylated gelatin (GelMA). The wound healing process of the old mice is remarkably accelerated, the wound area is small, the natural crust falling speed is higher, the wound reepithelization degree is higher, collagen arrangement is more compact and ordered, and hair papilla cell regeneration in the wound area is increased. The double-layer microneedle patch has a great application prospect in preparation of medicines for promoting wound healing of old people.

The invention belongs to the technical field of biological medicines, and discloses a hydrogel antibacterial dressing containing dragon's blood oil and a preparation method thereof. The hydrogel is characterized by comprising a three-dimensional hydrogel matrix formed by co-crosslinking methylacryloylated hyaluronic acid and gelatin, an in-situ compounded PEDOT: PSS / sodium carboxymethyl cellulose conductive nanofiber network, and dragon's blood oil microcapsules which are self-assembled and coated layer by layer by sodium alginate-chitosan and are modified by PEG (Polyethylene Glycol). Through the design, the dressing has stable conductivity (1.2-2.5 S / m), dual-phase drug release and excellent biocompatibility, synergistically promotes cell migration and microenvironment regulation and control under a low-intensity direct-current electric field, and significantly accelerates reepithelization and revascularization of diabetic wounds. According to the invention, the limitation of a dressing with a single function is overcome, the space-time coupling of a physical stimulation signal and a biochemical regulation signal is realized on a material architecture level, and an efficient, safe and clinically convertible novel treatment scheme is provided for the refractory wound of diabetes mellitus.

The invention relates to hydrogel design in the field of wound repair, in particular to polyamino acid, a preparation method of the polyamino acid, polyamino acid hydrogel and application of the polyamino acid hydrogel. The hydrogel prepared from polyamino acid provided by the invention is used for reactive oxygen species (ROS) regulation and hair follicle repair for efficient healing of chronic wounds, and compared with other clinically used wound dressings, the hydrogel can effectively reduce the ROS level of the wounds and provide a proper microenvironment for wound repair, so that cells are protected, and the healing effect of the wounds is improved. The traditional Chinese medicine composition can promote cell migration and proliferation, regulate the wound healing process, accelerate the conversion from the inflammation stage to the proliferation stage, promote vascular function reconstruction, collagen deposition and reepithelization, and solve the problem that chronic wounds are difficult to heal; moreover, the hair follicle generation promoting effect is achieved, and the problem that hair follicles are lost or generated slowly after wound healing can be solved.

The application discloses a kind of photothermal composite hydrogel patches and its preparation method and application, the hydrogel patch is with methacrylated gelatin (GelMA) and methacrylated hyaluronic acid (HAMA) as matrix, load carbonized niobium nanoparticles (Nb2CNPs) and verteporfin (VP), and is formed by 3D printing or casting forming three-dimensional grid porous structure, is abbreviated as VP-Nb2C@GelMA / HAMA (VNGH) hydrogel patch.The VNGH has good photo-thermal antibacterial, antioxidant, and re-epithelialization function, can significantly improve the quality of mouse diabetic wound healing, and provides a new idea and strategy for realizing scarless healing of diabetic wound.

The invention relates to a wound repair material, in particular to a three-dimensional printing composite hydrogel with antibacterial and immune regulation functions as well as a preparation method and application of the three-dimensional printing composite hydrogel. The three-dimensional printing composite hydrogel comprises a three-dimensional cross-linked network skeleton formed by gelatin methylacrylate and hyaluronic acid methylacrylate, the mixed charge network structure is formed by methacryloyloxyethyl trimethyl ammonium chloride and itaconic acid and contains cations and carboxyl; wherein the hybrid charge network structure is fixed in the three-dimensional cross-linked network skeleton through covalent bonds. Compared with the prior art, the problems that existing hydrogel is single in function and lacks internal antibacterial and inflammation regulation capacity, and printability is poor due to insufficient rheological property are solved. According to the scheme, through the structure-function integrated design, the material can have multiple internal functions of contact antibiosis, inflammatory reaction relief, angiogenesis promotion and the like under the condition that exogenous drugs are not needed, and wound reepithelization acceleration and tissue repair and collagen reconstruction promotion are facilitated.