32 results about "Re-epithelialization" patented technology

Methods for the therapeutic treatment of epithelial wounds in mammals comprising administering to a mammal afflicted with an epithelial wound a therapeutically effective amount of a galectin-3 protein and / or a galectin-7 protein are provided. Pharmaceutical compositions comprising a pharmaceutically suitable carrier or diluent and as an active agent a galectin-3 protein and / or a galectin-7 protein are also provided.

The invention discloses a skin wound dressing with the antimicrobial property and a preparation method of the skin wound dressing, belonging to the technical field of biomedical engineering. The skin wound dressing provided by the invention is of the spongy porous membrane structure, specifically, a sodium alginate fibrous membrane is prepared by taking sodium alginate as a base material through a freeze drying method, then biological antimicrobial peptide Cys-KR12 is fixed to the fibrous membrane through a surface finishing method, and thus the skin wound dressing is prepared. The skin wound dressing prepared by adopting the method provided by the invention is simple in preparation method and easily available in raw materials, the defects that the drug tolerance is generated, the functions are single, and the like after the existing antimicrobial dressing is used for a long term are overcome, and the skin wound dressing provided by the invention has low immunogenicity, good biocompatibility, good antimicrobial property and good induction repairing function, can promote the healing of the wound surface and the growth of epithelial cells, and is beneficial for the re-epithelialization of the wound surface.

The invention is a discrete patch suitable for use in the treatment of a viral lesion, which patch includes a backing layer and an adhesive layer, where the adhesive layer is substantially free of hydrocolloid particles, and the patch has a thickness ranging from about 10 microns to about 1,500 microns and is substantially free of topical anti-acne agents, and methods for treating viral lesions where a discrete patch of the invention is applied to a viral lesion and maintained in contact therewith for a time effective to substantially complete re-epithelialization of the lesion.

The invention discloses a fibrin hydrogel stent loaded with human umbilical cord mesenchymal stem cells and application of the fibrin hydrogel stent, and belongs to the technical field of hydrogel. The human umbilical cord mesenchymal stem cells are cultured until the density reaches 70 percent to 80 percent; treatment is performed by using a cell digestion solution, and the human umbilical cord mesenchymal stem cells are resuspended by using a fibrin raw solution to obtain a human umbilical cord mesenchymal stem cell-fibrin raw solution mixed solution; and a thrombin solution is added into the mixed solution to obtain the fibrin hydrogel stent loaded with the human umbilical cord mesenchymal stem cells. Through combined application of the fibrin hydrogel stent and the human umbilical cordmesenchymal stem cells, the healing speed of a wound is obviously accelerated; the re-epithelialization is promoted; the inflammatory response is inhibited; the angiogenesis is promoted; the scar formation is reduced; the wound is healed to be close to normal skin tissues to a greater extent; and the treatment requirement is met.

A wound-healing and hemostatic sponge of squid ink polysaccharide / chitosan, a preparation method and use thereof are provided. The sponge comprises squid ink polysaccharide and chitosan as carriers and calcium chloride as an initiator for blood coagulation, and the wound-healing and hemostatic sponge of squid ink polysaccharide / chitosan is achieved via a lyophilization technology. Particularly, the preparation method comprises slowly adding a squid ink polysaccharide solution to a chitosan solution to form a uniformly mixed solution, adding a calcium chloride solution to the uniformly mixed solution, forming a gel followed by the gel being frozen and then lyophilized, and achieving the product. The wound-healing and hemostatic sponge has a good absorbing-errhysis effect, a good pro-coagulant effect, fast hemostasis and a complete hemostatic effect, without secondary re-hemorrhage. Moreover, the wound-healing and hemostatic sponge can promote wound healing, re-epithelialization and repair of epidermis and dermis, with strong wound-healing ability, good biocompatibility, weak toxic side effects and irritation, belonging to a novel wound-healing and hemostatic material. Moreover, the preparation method is reliable, fast, and low cost.

Disclosed are methods of polarizing macrophages to exhibit M2 phenotype, including introducing an effective amount of gingiva-derived mesenchymal stem cells to an environment comprising a population of macrophages such that the macrophages are in fluid communication with the gingiva-derived mesenchymal stem cells. Also disclosed are methods of promoting cutaneous wound healing including administering to a patient an effective amount of human gingiva-derived mesenchymal stem cells, thereby resulting in at least one of accelerated wound closure, rapid re-epithelialization, improved angiogenesis and improved tissue remodeling relative to untreated controls. Also disclosed are methods for attenuating contact hypersensitivity in a patient, the methods including administering to a patient an effective amount of human gingiva-derived mesenchymal stem cells at a time at least timeframe selected from the group consisting of before sensitization, after sensitization and before challenge and after challenge, thereby attenuating contact hypersensitity.

The invention is a discrete patch suitable for use in the treatment of a viral lesion, which patch includes a backing layer and an adhesive layer, where the adhesive layer is substantially free of hydrocolloid particles, and the patch has a thickness ranging from about 10 microns to about 1,500 microns and is substantially free of topical anti-acne agents, and methods for treating viral lesions where a discrete patch of the invention is applied to a viral lesion and maintained in contact therewith for a time effective to substantially complete re-epithelialization of the lesion.

The invention provides sipunculus nudus zymolytic protein peptide powder having effect of promoting trauma healing. Sipunculus nudus has efficacy of immunoregulation, bacteria resisting, inflammationresisting and peripheral analgesia; the protein peptide powder can stop bleeding of a traumatic part and has antibacterial and anti-inflammatory effects; an organism takes the protein peptide powder in to induce generation of protein peptide Pro-Hyp to affect fibroblast growth so as to promote collagen synthesis at a wound part and wound healing to prevent abnormal scar formation. During the process, the protein peptide powder has effect of promoting proliferation and migration of endotheliocytes and keratinocytes so as to promote vascularization, provide nutritional substance and oxygen for the wound and promote epidermal reepithelialization to quickly form a closed space to prevent infection.

The invention belongs to the biomedical engineering field, and relates to a trachea substitute and a preparation method therefor. The trachea substitute has a porous and three-dimensional network structure, is a tubular, tegular or Y-type decellularized biomaterial, and comprises biological materials of allogeneic or heterogeneous sources. There are holes on the outer surface and the outer surface is seamed with spiral polyester materials. Experiments show that the trachea substitute has good biocompatibility with tissues, has no antigen interference effects, can perform revascularization and re-epithelization rapidly, has good air impermeability, ductility and elasticity, and can prevent collapse during breath. The trachea substitute can be made into different shapes and specifications, is suitable for tracheal reconstructive operations of tubular trachea substitution, juga molding, tracheal patching and the like. The trachea substitute is especially suitable for post-operation trachea substitution of trachea tumours, trachea trauma, benign tracheal stenosis, tumour invasion, congenital trachea malformation and the like.

The invention relates to a process for obtaining a cellular sprinkling compound and to the respective method of application to provide a therapeutic treatment for skin injuries, based on the implantation, by sprinkling and / or spraying, of human mesenchymal stem cells and microvascular endothelial cells that have been pre-expanded in vitro and resuspended in a regenerative solution for cellular implantation of biocompatible biomaterials. The solution is formed by blood plasma rich in growth factors obtained from the patient to be treated and, in some cases, by medical-grade type I collagen and by medical-grade hyaluronic acid, which potentiates the regeneration, re-epithelialization, and reconstruction of skin tissue.

The present invention provides novel uses for CXCR4 antagonists, including specifically peptides of the T-140 family, in the treatment of skin burns and other injuries. The invention further provides methods for increasing epithelialization in a subject in need thereof, and for preventing or inhibiting fibrosis and excessive scar formation, using peptide inhibitors of the T-140 family as well as other CXCR4 antagonists.

Compositions for topical application based on natural products, specifically dried pulverized plants suspended in bees honey, in which the ingredients are combined in proportions predetermined to provide three different types of preparations to be applied at various stages of repair and regeneration of injured tissues. The application of the compositions shortens the stages of healing in the treatment of acute and chronic injuries of any degree of depth and breadth, and of any etiology.The properties of the preparations include an hemorheological and systemic vascular activity which extends for a certain period even after the end of topical treatment.

A method of healing a wound including contacting a wound edge with a tropoelastin or elastin derived peptide in conditions for enabling a sustained contact of the tropoelastin with the wound edge for a time period for enabling re-epithelialization of the wound.

Disclosed are methods of polarizing macrophages to exhibit M2 phenotype, including introducing an effective amount of gingiva-derived mesenchymal stem cells to an environment comprising a population of macrophages such that the macrophages are in fluid communication with the gingiva-derived mesenchymal stem cells. Also disclosed are methods of promoting cutaneous wound healing including administering to a patient an effective amount of human gingiva-derived mesenchymal stem cells, thereby resulting in at least one of accelerated wound closure, rapid re-epithelialization, improved angiogenesis and improved tissue remodeling relative to untreated controls. Also disclosed are methods for attenuating contact hypersensitivity in a patient, the methods including administering to a patient an effective amount of human gingiva-derived mesenchymal stem cells at a time at least timeframe selected from the group consisting of before sensitization, after sensitization and before challenge and after challenge, thereby attenuating contact hypersensitivity.

The invention relates to the technical field of sponge scaffolds, in particular to a preparation method of a salidroside-collagen sponge scaffold and application thereof in skin wound healing. The preparation method of the salidroside-collagen sponge scaffold comprises the steps of I, preparing a collagen mixture; II, injecting into a culture plate; and III, lyophilizing. According to the preparation method, collagen is used as a natural material and drug carrier to construct the salidroside-collagen sponge scaffold, and the salidroside-collagen sponge scaffold prepared has the advantages of high interconnectivity and porosity, has no influence on drug release, and has good skin wound healing effect. The salidroside-collagen sponge scaffold was confirmed by animal experiments to have goodeffect on skin wound healing, references can be provided for basic research and clinical application of wound healing, the scaffold has the best re-epithelialization effect, and therefore, the salidroside-collagen sponge scaffold can promote skin wound healing and skin re-epithelialization.

The invention relates to the technical field of sponge scaffolds, in particular to a preparation method of a salidroside-collagen sponge scaffold and its application in skin wound repair. The preparation method of the salidroside-collagen sponge scaffold comprises: step 1, preparing a collagen mixture; step 2, injecting into a culture plate; and step 3, freeze-drying. The preparation method uses collagen as a natural material and a drug carrier to construct a salidroside-collagen sponge scaffold, and the prepared salidroside-collagen sponge scaffold has the advantages of high interconnectivity and porosity, and thus does not It affects drug release and has the advantage of good skin wound repair effect. And through animal experiments, it has been confirmed that salidroside-collagen sponge scaffold has a good effect on skin wound repair, which can provide reference for basic research and clinical application of wound repair, and its re-epithelialization effect is the best. Therefore, Rhodiola Glycoside-collagen sponge scaffold can promote skin wound repair and skin re-epithelialization.

The invention provides a human umbilical cord mesenchymal stem cell preparation and application thereof. According to the cell preparation, fibrous protein gel is used as a scaffold during cell transplantation, a three-dimensional environment which is more beneficial to survival is provided for hUC-MSCs cells, and the long-time repair promoting effect can be achieved. The invention further relates to the human mesenchymal stem cells with green fluorescence labels and an application of the human mesenchymal stem cells. Particularly, hUC-MSCs are marked by adopting EGFP, so that in-vivo tracing of transplanted cells and exploration of an action mechanism are realized. In addition, EGFP is adopted for hUC-MSCs, and it is verified that the hUC-MSCs cell preparation provided by the invention can remarkably accelerate healing of diabetic wounds and promote reepithelization.

The invention discloses a new application of a PD-L1 exosome. A new role of the PD-L1 exosome is researched at the cellular level and the animal level. An experiment finds that when the PD-L1 exosomeis applied to a novel thermosensitive gel for a damaged skin surface, the positive T cell activity can be reduced, the secretion of IL-6 and TNF-a is reduced, the expression of growth factors, such asTGF-beta and VEGF-A is increased, wound contraction and wound re-epithelization are significantly promoted, and wound healing is accelerated. The PD-L1 exosome can be used for preparing a preparationfor treating chronic ulcers and / or inflammatory diseases through inhibiting an excessive immune response of T cell activation (including proliferation or release of the growth factors) in peripheralblood mononuclear cells (PBMC) to damaged tissue, and has a relatively great application prospect.

The present invention relates to the strain of Lactobacillus plantarum NCIMB 43029, the strain of Lactobacillus acidophilus NCIMB 43030, a composition with both antibacterial and re- epithelializing action which includes Lactobacillus plantarum and Lactobacillus acidophilus and optionally Streptococcus thermophilus and / or Bacillus amyloliquefaciens intended to be used as a medicament for the re-epithelialisation of lesions or wounds, a bandage comprising said composition and kits comprising such a composition.

Pharmaceutical compositions for the treatment of bacterial skin infections, burns and wounds are disclosed. The pharmaceutical composition comprises an active ingredient, preferably mupirocin; a biopolymer, preferably chitosan, having a molecular weight in the range of 250,000 Da to 600,000 Da, and having a degree of deacetylation of not less than 80%; hydrolyzed collagen, preferably Type I, having a molecular weight in the range of 3 kDa to 6 kDa, and an ointment base comprising an emulsifier, a diol, and a solubilizing agent. The present invention has better stability than existing ointments, which achieves skin healing and reepithelization faster than existing treatments, thereby shortening hospitalization time.

As described herein, leptin treatment significantly increased wound contraction and epithelial regeneration while reducing granulation tissue and wound area, consistent with a healing augmentation effect. Specifically, in leptin-treated wounds, the inventor found increased expression of smooth muscle-actin (-SMA) and collagens I, III and IV. Taken together, the inventor's results indicate that a major functional theme for the accelerated wound healing action of leptin consists of the acute, local induction of genes whose expression are critical for repair and contraction. Thus, the invention relates to methods and compositions for the promotion and / or acceleration of wound repair, re-epithelialization, wound contraction and decrease of granulation tissue by administering leptin to the subject, as well as methods for studying this process.

A formulation of a discrete liquid patch for the treatment of cold sores or viral lesions, including a combination of the adhesive layer and an active compound / ingredient such as antiviral agents, and / or amino acids, and / or minerals / vitamins. The adhesive layer of the discrete patch is substantially free of hydrocolloid particles. The adhesive layer does not include any backing layer to maintain the compliance of the patch with a thickness ranging from 5 microns to 1,300 microns. Wherein, the discrete patch of the invention is configured to apply to a viral lesion to maintain in contact therewith for a time effective to substantially complete re-epithelialization of the lesion.

The invention discloses a novel non-woven fabric wound dressing and a preparation method and application thereof. The wound dressing is prepared from modified polyester fibers, modified chitosan fibers and modified calcium alginate fibers through a spunlace or needling non-woven processing procedure, wherein the modified polyester fibers are 20-40 parts by mass, the modified chitosan fibers are 10-20 parts by mass, and the modified calcium alginate fibers are 10-40 parts by mass; the modified polyester fibers contain 5-10 wt% of nano silver, the modified chitosan fibers contain 0.1-1 wt% of copper ions, and the modified calcium alginate fibers contain 0.1-2 wt% of zinc ions; and the invention also discloses the application of the novel non-woven fabric wound dressing in skin wound covering dressings. According to the wound dressing, the antibacterial effect can penetrate through the whole wound healing process, the effects of promoting angiogenesis, promoting reepithelization and the like can be achieved, and therefore the effective promoting or auxiliary accelerating effect is achieved in the wound healing process.

A wound-healing and hemostatic sponge of squid ink polysaccharide / chitosan, a preparation method and use thereof are provided. The sponge comprises squid ink polysaccharide and chitosan as carriers and calcium chloride as an initiator for blood coagulation, and the wound-healing and hemostatic sponge of squid ink polysaccharide / chitosan is achieved via a lyophilization technology. Particularly, the preparation method comprises slowly adding a squid ink polysaccharide solution to a chitosan solution to form a uniformly mixed solution, adding a calcium chloride solution to the uniformly mixed solution, forming a gel followed by the gel being frozen and then lyophilized, and achieving the product. The wound-healing and hemostatic sponge has a good absorbing-errhysis effect, a good pro-coagulant effect, fast hemostasis and a complete hemostatic effect, without secondary re-hemorrhage. Moreover, the wound-healing and hemostatic sponge can promote wound healing, re-epithelialization and repair of epidermis and dermis, with strong wound-healing ability, good biocompatibility, weak toxic side effects and irritation, belonging to a novel wound-healing and hemostatic material. Moreover, the preparation method is reliable, fast, and low cost.

The invention relates to the technical field of blood products, and discloses a preparation method of leukocyte-rich and platelet-rich plasma (L-PRP), comprising the steps of sampling blood and preparing L-PRP; the invention also discloses an application of the L-PRP in wound treatment. The platelet content of the prepared L-PRP is about 4 times that of whole blood, the L-PRP is rich in leukocytes and interleukin 6 (IL-6), and it is found that the L-PRP can promote keratinocyte migration and accelerate wound reepithelization by up-regulating the IL-6 transcriptional level and activating signal transduction and transcriptional activation protein 3 (STAT3); according to the prepared L-PRP and the research on the L-PRP, classification and application scenes of platelet-rich plasma (PRP) are refined, a new mechanism of promoting wound healing by the L-PRP is found, a new solution is provided for clinically solving refractory wounds, and the L-PRP has important clinical practical significance.