Composition with antibacterial and re-epithelializing action including probiotics

A technology of compositions and strains, applied in antibacterial drugs, medical preparations containing active ingredients, bacteria, etc., can solve problems such as unclear results

Pending Publication Date: 2022-02-25
MENDES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although the use of probiotics to treat skin changes has been studied, the results obtained do not appear to be definitive and / or entirely satisfactory

Method used

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  • Composition with antibacterial and re-epithelializing action including probiotics
  • Composition with antibacterial and re-epithelializing action including probiotics
  • Composition with antibacterial and re-epithelializing action including probiotics

Examples

Experimental program
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Effect test

preparation example Construction

[0139] For the preparation of bacterial extracts, 1 g of each lyophilized bacterial stock resuspended in phosphate-buffered saline (PBS, Euro Clone, West Yorkshire, UK) was centrifuged at 8,600 x g, washed twice, and resuspended. Sonication (30 min, alternating 10 sec sonications with 10 sec pauses) was performed in 10 ml PBS and using a Vibracell sonicator (Sonic and Materials, Danbury, CT).

[0140] Bacterial cell fragmentation was verified by measuring the absorbance of the samples at 590 nm (Eppendorf Hamburg, Germany) before and after each sonication step. Samples were then centrifuged at 17,949 xg, and the supernatant was filtered using a 0.22 mm pore filter (Corning Incorporated, Corning, NY, USA) to remove any remaining whole bacteria. Protein analysis by BioRad DC (BioRad, Hercules, CA) using bovine serum albumin (BSA, SigmaAldrich, Saint Louis, MO, USA) )) was used as a standard to determine the total protein content. For in vitro experiments, the bacterial prepara...

Embodiment 1

[0155] Example 1 - Application of a composition of the invention in powder form to ulcers affected by infection due to diabetes and the foot of a female patient with a toe amputation wound

[0156] The efficacy of compositions comprising Lactobacillus plantarum NCIMB 43029 as previously identified, Lactobacillus acidophilus NCIMB 43030 as previously identified and known Streptococcus thermophilus as previously identified has been studied on diabetic ulcers and surgical wounds.

[0157] In particular, an 83-year-old woman with critical limb ischemia (CLI) was brought to our attention with an ulcerated skin lesion on her right leg extending to the 2nd and 3rd toes. His medical history revealed type II diabetes, systemic arterial hypertension, ischemic heart disease (previous coronary artery bypass surgery), and atrial fibrillation (AF). She was also an active smoker (20 cigarettes / day for 20 years). Based on clinical observations, bilateral femoral pulses were present, but ...

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Abstract

The present invention relates to the strain of Lactobacillus plantarum NCIMB 43029, the strain of Lactobacillus acidophilus NCIMB 43030, a composition with both antibacterial and re- epithelializing action which includes Lactobacillus plantarum and Lactobacillus acidophilus and optionally Streptococcus thermophilus and / or Bacillus amyloliquefaciens intended to be used as a medicament for the re-epithelialisation of lesions or wounds, a bandage comprising said composition and kits comprising such a composition.

Description

[0001] field of invention [0002] The present invention relates to Lactobacillus plantarum strain NCIMB 43029; Lactobacillus acidophilus strain NCIMB 43030; a composition having antibacterial and re-epithelializing effects, said composition comprising Lactobacillus plantarum and Lactobacillus acidophilus and optionally comprising Streptococcus thermophilus and / or Bacillus amyloliquefaciens, said composition being used as a medicinal product, especially in the process of re-epithelialization of an injury or wound; and comprising said Composition for bandage or kit. Background technique [0003] Successful skin regeneration is a major challenge because injury or wound healing is a highly dynamic and complex process that involves many phases, starting with the hemostasis and coagulation phase, forming a temporary wound matrix, followed by inflammation, re-epithelialization, and tissue remodeling. stage of recovery. [0004] When an injury or wound becomes chronic, it becomes m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61K9/00A61P17/02A61P31/04A61P31/02A61P3/10A61P31/10A61P1/02A61P15/02A61P1/00A61P1/04A61L26/00A61L15/40A61F13/00A61K35/744A61K35/742C12R1/25C12R1/23
CPCC12N1/20A61K35/747A61K35/744A61K35/742A61K9/0053A61K9/0014A61K9/0034A61P17/02A61P31/04A61P31/02A61P3/10A61P31/10A61P1/02A61P15/02A61P1/00A61P1/04A61L26/0057A61L15/40A61F13/00063A61K2300/00A61K2035/115A61K35/644C12R2001/23C12N1/205C12R2001/25C12R2001/46C12R2001/07
Inventor G·桑蒂尼
Owner MENDES
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