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Preparation method of salidroside-collagen sponge scaffold and application thereof in skin wound healing

A technology of collagen sponge and salidroside, applied in medical science, bandages, etc., can solve the problems of poor skin wound repair effect, achieve the best effect, low production cost, and simple equipment

Active Publication Date: 2020-02-28
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen is the main component of the extracellular matrix. It has strong biological activity and biological function. It is also a natural tissue scaffold material and is widely used in wound repair. However, the effect of skin wound repair is still not good and needs continuous improvement.

Method used

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  • Preparation method of salidroside-collagen sponge scaffold and application thereof in skin wound healing
  • Preparation method of salidroside-collagen sponge scaffold and application thereof in skin wound healing
  • Preparation method of salidroside-collagen sponge scaffold and application thereof in skin wound healing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of salidroside-collagen sponge support, it comprises the following steps:

[0031] Step 1, prepare the collagen mixture: mix the collagen solution and salidroside evenly to obtain the collagen mixture; in the present embodiment, the concentration of the collagen solution is 8mg / g; in the present embodiment, the collagen solution and the rhodiola The mass ratio of Tianglycoside is 38.5:0.2;

[0032] Wherein, the preparation method of the collagen solution is as follows: the tissue of an animal rich in collagen is extracted by an acid enzyme combination method to obtain a crude extract, and then the crude extract is purified by centrifugation, salting out and ultrafiltration in sequence, That is, pure collagen solution is obtained;

[0033] Step 2, injecting into the culture plate: inject the collagen mixture prepared in the step 1 into the culture plate;

[0034] Step 3, freeze-drying: the collagen mixture injected into the culture plate in step 2 ...

Embodiment 2

[0037] A preparation method of salidroside-collagen sponge support, it comprises the following steps:

[0038] Step 1, prepare the collagen mixture: mix the collagen solution and salidroside evenly to obtain the collagen mixture; in the present embodiment, the concentration of the collagen solution is 6 mg / g; in the present embodiment, the collagen solution and the rhodiola The mass ratio of Tianglycoside is 30:0.05;

[0039] Wherein, the preparation method of the collagen solution is as follows: the tissue of an animal rich in collagen is extracted by an acid enzyme combination method to obtain a crude extract, and then the crude extract is purified by centrifugation, salting out and ultrafiltration in sequence, That is, pure collagen solution is obtained;

[0040] Step 2, injecting into the culture plate: inject the collagen mixture prepared in the step 1 into the culture plate;

[0041] Step 3, freeze-drying: the collagen mixture injected into the culture plate in step 2 ...

Embodiment 3

[0044] A preparation method of salidroside-collagen sponge support, it comprises the following steps:

[0045] Step 1, prepare the collagen mixture: mix the collagen solution and salidroside evenly to obtain the collagen mixture; in the present embodiment, the concentration of the collagen solution is 10mg / g; in the present embodiment, the collagen solution and the rhodiola The mass ratio of Tianglycoside is 45:0.5;

[0046] Wherein, the preparation method of the collagen solution is as follows: the tissue of an animal rich in collagen is extracted by an acid enzyme combination method to obtain a crude extract, and then the crude extract is purified by centrifugation, salting out and ultrafiltration in sequence, That is, pure collagen solution is obtained;

[0047] Step 2, injecting into the culture plate: inject the collagen mixture prepared in the step 1 into the culture plate;

[0048] Step 3, freeze-drying: the collagen mixture injected into the culture plate in step 2 i...

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Abstract

The invention relates to the technical field of sponge scaffolds, in particular to a preparation method of a salidroside-collagen sponge scaffold and application thereof in skin wound healing. The preparation method of the salidroside-collagen sponge scaffold comprises the steps of I, preparing a collagen mixture; II, injecting into a culture plate; and III, lyophilizing. According to the preparation method, collagen is used as a natural material and drug carrier to construct the salidroside-collagen sponge scaffold, and the salidroside-collagen sponge scaffold prepared has the advantages of high interconnectivity and porosity, has no influence on drug release, and has good skin wound healing effect. The salidroside-collagen sponge scaffold was confirmed by animal experiments to have goodeffect on skin wound healing, references can be provided for basic research and clinical application of wound healing, the scaffold has the best re-epithelialization effect, and therefore, the salidroside-collagen sponge scaffold can promote skin wound healing and skin re-epithelialization.

Description

technical field [0001] The present invention relates to the technical field of sponge support, in particular to a preparation method of salidroside-collagen sponge support and its application in skin wound repair. Background technique [0002] Collagen is a natural high-molecular protein, mainly distributed in animal bones, tendons, ligaments, skin and cornea, and is the most abundant protein in animals. Collagen extraction methods mainly include neutral salt dissolution method, acid dissolution method, alkali dissolution method and enzymatic method. Collagen solutions for commercial applications can be used to prepare various gels, collagen membranes and scaffolds, etc. to be used in tissue engineering materials, drug controlled release carriers, and wound repair and healing. Collagen has been widely used as a biomedical material. However, pure collagen has defects of low interconnection and porosity, which easily affect drug release. [0003] Skin wound repair is a dynam...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0033A61L26/0061A61L26/0066A61L26/0085A61L2300/232A61L2300/412
Inventor 周艳芳彭新生温玉莹周艳星钟燕南梁佳欣周竞扬王贤君符腾越周清清范志强李玉玲
Owner GUANGDONG MEDICAL UNIV
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