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A preparation method of salidroside-collagen sponge scaffold and its application in skin wound repair

A collagen sponge and salidroside technology, applied in bandages, medical science and other directions, can solve the problems of poor skin wound repair and other problems, achieve the best effect, low production cost, and promote the effect of skin re-epithelialization

Active Publication Date: 2021-10-08
东莞胶原生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen is the main component of the extracellular matrix. It has strong biological activity and biological function. It is also a natural tissue scaffold material and is widely used in wound repair. However, the effect of skin wound repair is still not good and needs continuous improvement.

Method used

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  • A preparation method of salidroside-collagen sponge scaffold and its application in skin wound repair
  • A preparation method of salidroside-collagen sponge scaffold and its application in skin wound repair
  • A preparation method of salidroside-collagen sponge scaffold and its application in skin wound repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of salidroside-collagen sponge support, it comprises the following steps:

[0031] Step 1, prepare the collagen mixture: mix the collagen solution and salidroside evenly to obtain the collagen mixture; in the present embodiment, the concentration of the collagen solution is 8mg / g; in the present embodiment, the collagen solution and the rhodiola The mass ratio of Tianglycoside is 38.5:0.2;

[0032] Wherein, the preparation method of the collagen solution is as follows: the tissue of an animal rich in collagen is extracted by an acid enzyme combination method to obtain a crude extract, and then the crude extract is purified by centrifugation, salting out and ultrafiltration in sequence, That is, pure collagen solution is obtained;

[0033] Step 2, injecting into the culture plate: inject the collagen mixture prepared in the step 1 into the culture plate;

[0034] Step 3, freeze-drying: the collagen mixture injected into the culture plate in step 2 ...

Embodiment 2

[0037] A preparation method of salidroside-collagen sponge support, it comprises the following steps:

[0038] Step 1, prepare the collagen mixture: mix the collagen solution and salidroside evenly to obtain the collagen mixture; in the present embodiment, the concentration of the collagen solution is 6 mg / g; in the present embodiment, the collagen solution and the rhodiola The mass ratio of Tianglycoside is 30:0.05;

[0039] Wherein, the preparation method of the collagen solution is as follows: the tissue of an animal rich in collagen is extracted by an acid enzyme combination method to obtain a crude extract, and then the crude extract is purified by centrifugation, salting out and ultrafiltration in sequence, That is, pure collagen solution is obtained;

[0040] Step 2, injecting into the culture plate: inject the collagen mixture prepared in the step 1 into the culture plate;

[0041] Step 3, freeze-drying: the collagen mixture injected into the culture plate in step 2 ...

Embodiment 3

[0044] A preparation method of salidroside-collagen sponge support, it comprises the following steps:

[0045] Step 1, prepare the collagen mixture: mix the collagen solution and salidroside evenly to obtain the collagen mixture; in the present embodiment, the concentration of the collagen solution is 10mg / g; in the present embodiment, the collagen solution and the rhodiola The mass ratio of Tianglycoside is 45:0.5;

[0046] Wherein, the preparation method of the collagen solution is as follows: the tissue of an animal rich in collagen is extracted by an acid enzyme combination method to obtain a crude extract, and then the crude extract is purified by centrifugation, salting out and ultrafiltration in sequence, That is, pure collagen solution is obtained;

[0047] Step 2, injecting into the culture plate: inject the collagen mixture prepared in the step 1 into the culture plate;

[0048] Step 3, freeze-drying: the collagen mixture injected into the culture plate in step 2 i...

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Abstract

The invention relates to the technical field of sponge scaffolds, in particular to a preparation method of a salidroside-collagen sponge scaffold and its application in skin wound repair. The preparation method of the salidroside-collagen sponge scaffold comprises: step 1, preparing a collagen mixture; step 2, injecting into a culture plate; and step 3, freeze-drying. The preparation method uses collagen as a natural material and a drug carrier to construct a salidroside-collagen sponge scaffold, and the prepared salidroside-collagen sponge scaffold has the advantages of high interconnectivity and porosity, and thus does not It affects drug release and has the advantage of good skin wound repair effect. And through animal experiments, it has been confirmed that salidroside-collagen sponge scaffold has a good effect on skin wound repair, which can provide reference for basic research and clinical application of wound repair, and its re-epithelialization effect is the best. Therefore, Rhodiola Glycoside-collagen sponge scaffold can promote skin wound repair and skin re-epithelialization.

Description

technical field [0001] The invention relates to the technical field of sponge scaffolds, in particular to a preparation method of a salidroside-collagen sponge scaffold and its application in skin wound repair. Background technique [0002] Collagen is a natural high-molecular protein, which is mainly distributed in the bones, tendons, ligaments, skin and cornea of ​​animals, and is the most abundant protein in animals. Collagen extraction methods mainly include neutral salt dissolution method, acid dissolution method, alkali dissolution method and enzymatic method. Collagen solutions for commercial applications can be used to prepare various gels, collagen membranes and scaffolds, which are used in tissue engineering materials, drug controlled release carriers, wound repair and healing, and the like. Collagen has been widely used as a biomedical material. However, pure collagen has defects of low interconnection and porosity, which easily affect drug release. [0003] Ski...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/0033A61L26/0061A61L26/0066A61L26/0085A61L2300/232A61L2300/412
Inventor 周艳芳彭新生温玉莹周艳星钟燕南梁佳欣周竞扬王贤君符腾越周清清范志强李玉玲
Owner 东莞胶原生物科技有限公司
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