Eyedrop for treating photochemical injury of eyes and preparation method of eyedrop

Abstract

The invention relates to the technical field of traditional Chinese medicines, and discloses eye drops for treating photochemically damaged eyes and a preparation method thereof. The eyedrops are prepared from the following raw materials: rhizoma polygonati, musk, flos chrysanthemi indici, semen cassiae, honeysuckle flowers, cortex phellodendri, herba houttuyniae, pearl powder, cortex fraxini, flatstem milkvetch seeds, rhizoma seu radix notopterygii and carthamin yellow. The traditional Chinese medicine composition is reasonable in component compatibility and synergistic in interaction, conforms to the traditional Chinese medicine theory, has the effects of clearing heat and purging fire, clearing liver and improving vision, clearing away heat and toxic materials and inducing diuresis to alleviate edema, can treat various eye diseases and discomfort caused by electronic equipment, and can also remarkably recover the activity of SOD and inhibit generation of MDA, so that retina lipid peroxidation caused by illumination is relieved, and the retina lipid peroxidation effect is improved. The composition has a good protection effect on the retina damaged by photochemistry, and the curative effect is remarkable.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and more particularly to an eye drop for treating photochemically damaged eyes and a preparation method thereof. Background Art

[0002] With the development of modern society, eye diseases have become a common disease among the general public. Due to various bad habits of people, such as sitting in front of the computer for a long time, browsing the mobile phone on the sofa for a long time, and facing electronic devices all day, various eye problems often occur, including dry and swollen eyes, photophobia, red bloodshot eyes, lens and vitreous opacities, floaters, pseudomyopia, eye fatigue, blurred vision, etc.

[0003] The retina is an important tissue structure in the visual organ. Its structure and function are very complex and delicate. It is both a light receptor and a light conductor, and is the first key to forming vision. Only when light reaches the retina with normal structure and function can a visual image be formed. However, when the intensity of light and the duration of illumination exceed the protection range of the eye tissue to the retina, it will cause damage to the retina and cause changes in the retinal cell structure. Existing studies believe that there are three types of retinal light damage: thermal damage, mechanical damage, and photochemical damage. Retinal photochemical damage is the most common form of retinal light damage. Its pathological mechanism is that photooxidative stress induces apoptosis of photoreceptor cells, and visible light mainly causes retinal photochemical damage.

[0004] The pathogenesis of retinal photochemical damage is mainly related to oxygen free radicals and lipid peroxidation. Photooxidative stress initiates retinal damage and maintains its pathological process, which is similar to retinal degenerative diseases. During retinal photochemical damage, the absorbed excess photons induce the massive production of free radicals, leading to lipid peroxidation, forming reactive oxygen mediators, promoting photoreceptor cell apoptosis, destroying the retinal structure, and ultimately causing visual impairment or complete loss. However, many eye drops on the market can only temporarily relieve symptoms, have poor protective effects on the retina, and are prone to drug dependence with long-term use. The efficacy will gradually weaken, and more seriously, it will lead to organic changes in the eyes and produce adverse consequences.

[0005] Therefore, how to improve an eye drop for treating photochemically damaged eyes and a preparation method thereof is a problem that needs to be solved urgently by those skilled in the art. Summary of the invention

[0006] In view of this, the present invention provides an eye drop for treating photochemically damaged eyes and a preparation method thereof.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] An eye drop for treating photochemical damage to the eyes, comprising the following ingredients: polygonatum, musk, wild chrysanthemum, cassia seed, honeysuckle, phellodendron, houttuynia cordata, pearl powder, qinpi, complanatus, notopterygium wilfordii, and safflower yellow pigment.

[0009] Preferably, the following raw materials are included in parts by weight: 80-100 parts of polygonatum, 60-70 parts of musk, 60-70 parts of wild chrysanthemum, 50-55 parts of cassia seed, 50-55 parts of honeysuckle, 45-50 parts of phellodendron, 40-50 parts of houttuynia cordata, 20-35 parts of pearl powder, 12-16 parts of qinpi, 8-12 parts of complanatus, 8-10 parts of notopterygium wilfordii, and 2-5 parts of safflower yellow.

[0010] Preferably, the following raw materials are included in parts by weight: 90 parts of polygonatum, 65 parts of musk, 65 parts of wild chrysanthemum, 52 parts of cassia seed, 52 parts of honeysuckle, 48 parts of phellodendron, 45 parts of houttuynia cordata, 25 parts of pearl powder, 15 parts of qinpi, 10 parts of complanatus, 9 parts of notopterygium, and 4 parts of safflower yellow.

[0011] As the same inventive concept as the above technical solution, the present invention also claims protection for a method for preparing the above eye drops, comprising the following steps:

[0012] (1) Weigh the raw materials according to the above weight parts and set aside;

[0013] (2) adding polygonatum, musk, wild chrysanthemum, cassia seed, honeysuckle, phellodendron, houttuynia cordata, pearl powder, qinpi, complanatus, and notopterygium wilfordii into a ball mill and grinding the powder, soaking the obtained powder in deionized water, and then performing heating microwave-assisted extraction 2 to 3 times, combining the extracts, filtering, and centrifuging to obtain the supernatant;

[0014] (3) subjecting the supernatant obtained in step (2) to ultrafiltration, and concentrating the obtained ultrafiltrate under reduced pressure to a relative density of 1.05 to 1.15;

[0015] (4) Deionized water is added to the concentrated ultrafiltrate, and then safflower yellow pigment and a pH adjuster are added to adjust the pH to 6.5 to 7.5, and sterilized to obtain eye drops.

[0016] Preferably, the average particle size of the powder in step (2) is 100-150 mesh, and the soaking time is 0.5-2h.

[0017] Preferably, the mass ratio of the powder to deionized water in step (2) is 1:6-10.

[0018] Preferably, in step (2), the power of the microwave is 800-1000W, the extraction temperature is 80-95°C, and the extraction time is 2-4h; and the centrifugation is performed at 500-800rpm for 8-15min.

[0019] Preferably, the molecular weight cut-off of the ultrafiltration treatment in step (3) is 30,000 Da, and the cut-off temperature is 30-45° C.

[0020] Preferably, the volume ratio of the ultrafiltrate to deionized water in step (4) is 1:10-12.

[0021] Preferably, the pH adjuster in step (4) is selected from a phosphate buffer and a borate buffer.

[0022] The effects of the raw materials in the eye drops of the present invention are as follows:

[0023] Polygonatum: sweet, neutral, enters the spleen, lung, and kidney meridians. The main effects are to nourish qi and yin, strengthen the spleen, moisturize the hair, and benefit the kidney. Pharmacological studies have shown that Polygonatum has the effects of immune stimulation, immune enhancement, anti-aging, anti-virus, anti-inflammatory, and can increase tear secretion and promote ocular surface healing. It mainly contains polysaccharides, steroidal saponins, anthraquinone compounds, alkaloids, cardiac glycosides, lignin, vitamins, and various amino acids. Among them, Polygonatum polysaccharide is its main pharmacologically active substance.

[0024] Musk: pungent and warm in nature. It enters the heart and spleen meridians. It is pungent, dispersing, warming and unblocking, with a fragrant aroma. It enters the heart and spleen meridians, is good at opening orifices and blocking, and relieves swelling and pain. It is a good medicine for opening orifices and refreshing the mind, and is suitable for treating closed syndrome and coma, regardless of cold or heat; it is also a good product for promoting blood circulation, unblocking menstruation, and relieving pain, and is suitable for treating various blood stasis syndromes, regardless of whether they are new or long-term.

[0025] Wild chrysanthemum: clears the liver and improves eyesight, clears away heat and detoxifies, and has strong anti-inflammatory and immunomodulatory effects. Wild chrysanthemum water extract can reduce inflammatory effects, inhibit the phagocytic function of macrophages, and increase the secretion of basal tears.

[0026] Cassia seed: sweet, bitter, salty, slightly cold, enters the liver and large intestine meridians. Cassia seed is bitter, slightly cold, and clears and drains, while sweet and nourishing lubricates. It enters the liver and kidney meridians, can clear liver fire, nourish kidney yin and improve eyesight, and is an essential medicine for red, swollen and painful eyes and dark and unclear eyes.

[0027] Honeysuckle: sweet and cold in nature. It enters the lung, stomach and large intestine meridians. Honeysuckle is sweet and cold, clearing and dispersing, clearing the liver and improving eyesight, clearing away heat and detoxifying, calming the liver and cooling blood.

[0028] Huangbai: bitter and cold in nature. It enters the kidney and bladder meridians. Huangbai is bitter and cold, and it is dry and sinks, entering the kidney and bladder meridians. It can clear away real heat (fire) and relieve heat toxicity, and it can also dry out dampness, remove dampness toxicity and relieve damp heat, and it can also clear kidney fire (phase fire) and relieve false heat. It is an important medicine for treating damp heat and fire toxicity.

[0029] Houttuynia cordata: pungent, slightly cold, enters the lung and bladder meridians. Houttuynia cordata is pungent and fragrant, slightly cold and can clear, enters the lung and bladder meridians. It clears away heat and detoxifies, effectively relieves eye discomfort caused by bacterial or viral infections, promotes diuresis and reduces swelling, and relieves eye fatigue and pain.

[0030] Pearl powder: calms the mind, improves eyesight, detoxifies and promotes muscle growth, removes cataracts and improves eyesight. The zinc element rich in pearl powder can promote the transmission of eye nerve axons, improve the macula, and promote the metabolism of melanocytes and squamous epithelial cells, which is extremely beneficial to the eyes. It is suitable for symptoms such as red, swollen and painful eyes, blurred vision, etc.

[0031] Fraxinus cortex: bitter, astringent, cold, enters the liver, gallbladder, and large intestine meridians. It has the effects of clearing away heat and dampness, astringing and stopping dysentery, stopping leukorrhea, and improving eyesight. It is used for damp-heat diarrhea, red and swollen eyes, and pannus. It mainly contains coumarin ingredients such as aesculin A, aesculetin, aesculetin, aesculin glycoside, etc.; it also contains phenols, saponins, tannins, etc.

[0032] Astragalus: sweet, warm, enters the liver and kidney meridians. Sweet, warm, tonic and astringent, not dry or strong, similar to Cuscuta but with stronger astringent effect. Enters the liver meridian, nourishes liver blood and improves eyesight.

[0033] Qianghuo: pungent, bitter, warm. Enters the bladder and kidney meridians. Qianghuo is pungent, dispersing, bitter, and dry, warming, ascending, and dispersing. It has a strong and intense Qi and mainly enters the bladder meridian and the kidney meridian. It can dispel wind and dredge collaterals, and can be used for external eye diseases caused by wind and heat fighting against each other and attacking the eyes.

[0034] Safflower yellow: has antioxidant, anti-inflammatory, anti-cancer, and antihypertensive effects. The antioxidant effect of safflower yellow is mainly achieved by scavenging oxygen free radicals, regulating NO, and mediating NMDA receptor expression. Safflower yellow exerts its anti-inflammatory effect by antioxidant effects, regulating NO synthesis, antagonizing PAF, and regulating immune responses. Safflower yellow can reduce a series of changes characterized by cell apoptosis induced by oxygen free radicals, such as changes in cell membrane integrity and permeability, changes in cell membrane channels and ion pump functions, protein oxidation, DNA breakage, etc., and can reduce retinal lipid peroxidation.

[0035] It can be seen from the above technical solution that, compared with the prior art, the present invention provides an eye drop for treating photochemically damaged eyes and a preparation method thereof, which has the following beneficial effects:

[0036] (1) The components of the present invention are reasonably formulated and synergistically enhance each other. They not only conform to the pharmacology of traditional Chinese medicine, but also have the effects of clearing away heat and purging fire, clearing the liver and improving eyesight, clearing away heat and detoxifying, promoting diuresis and reducing swelling. They can treat a variety of eye diseases and discomfort caused by electronic devices, and can also significantly restore the activity of SOD and inhibit the production of MDA, thereby reducing retinal lipid peroxidation caused by light, and have a good protective effect on the photochemically damaged retina, with significant therapeutic effects.

[0037] (2) The extraction method of the present invention can effectively extract the active ingredients of the raw materials, maximize the role of the raw materials, and produce a synergistic effect between multiple functions, thereby enhancing the analgesic effect. The extract is purified by ultrafiltration technology, which can not only remove suspended matter and water-soluble macromolecular impurities in the extract, but also retain the original effective ingredients to the greatest extent, that is, achieve the purpose of removing the coarse and retaining the fine. Compared with existing products on the market, the formula of the present invention has mild ingredients, high safety, no side effects, and will not cause dependence after long-term use. DETAILED DESCRIPTION

[0038] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] Example 1

[0040] An eye drop for treating photochemical eye damage, comprising the following raw materials, measured by weight: 80 parts of polygonatum, 60 parts of musk, 60 parts of wild chrysanthemum, 50 parts of cassia seed, 50 parts of honeysuckle, 45 parts of phellodendron, 40 parts of houttuynia, 20 parts of pearl powder, 12 parts of qinpi, 8 parts of complanatus, 8 parts of notopterygium, and 2 parts of safflower yellow.

[0041] The preparation method of the above eye drops comprises the following steps:

[0042] (1) Weigh the raw materials according to the above weight parts and set aside;

[0043] (2) adding polygonatum, musk, wild chrysanthemum, cassia seed, honeysuckle, phellodendron, houttuynia cordata, pearl powder, qinpi, complanatus, and notopterygium wilfordii into a ball mill and grinding them, soaking the obtained powder in deionized water, and then performing heating microwave-assisted extraction three times, combining the extracts, filtering, and centrifuging to obtain the supernatant;

[0044] (3) subjecting the supernatant obtained in step (2) to ultrafiltration, and concentrating the obtained ultrafiltrate under reduced pressure to a relative density of 1.1;

[0045] (4) adding deionized water to the concentrated ultrafiltrate, and then adding safflower yellow pigment and a pH adjuster, adjusting the pH to 7, and sterilizing to obtain eye drops;

[0046] The average particle size of the powder in step (2) is 120 mesh, and the soaking time is 1 hour;

[0047] The mass ratio of the powder to deionized water in step (2) is 1:8;

[0048] In step (2), the microwave power is 900W, the extraction temperature is 90°C, and the extraction time is 3h; the centrifugation is performed at 700rpm for 12min;

[0049] The ultrafiltration treatment in step (3) has a molecular weight cut-off of 30,000 Da and a cut-off temperature of 40° C.

[0050] The volume ratio of the ultrafiltrate to deionized water in step (4) is 1:10;

[0051] The pH regulator in step (4) is a phosphate buffer.

[0052] Example 2

[0053] An eye drop for treating photochemical eye damage, comprising the following raw materials, measured by weight: 100 parts of polygonatum, 70 parts of musk, 70 parts of wild chrysanthemum, 55 parts of cassia seed, 55 parts of honeysuckle, 50 parts of phellodendron, 50 parts of houttuynia, 35 parts of pearl powder, 16 parts of qinpi, 12 parts of complanatus, 10 parts of notopterygium, and 5 parts of safflower yellow.

[0054] The preparation method of the above eye drops comprises the following steps:

[0055] (1) Weigh the raw materials according to the above weight parts and set aside;

[0056] (2) adding polygonatum, musk, wild chrysanthemum, cassia seed, honeysuckle, phellodendron, houttuynia cordata, pearl powder, qinpi, complanatus, and notopterygium wilfordii into a ball mill and grinding them, soaking the obtained powder in deionized water, and then performing heating microwave-assisted extraction three times, combining the extracts, filtering, and centrifuging to obtain the supernatant;

[0057] (3) subjecting the supernatant obtained in step (2) to ultrafiltration, and concentrating the obtained ultrafiltrate under reduced pressure to a relative density of 1.15;

[0058] (4) adding deionized water to the concentrated ultrafiltrate, and then adding safflower yellow pigment and a pH adjuster to adjust the pH to 7.5, sterilizing, and obtaining eye drops;

[0059] The average particle size of the powder in step (2) is 150 mesh, and the soaking time is 2 hours;

[0060] The mass ratio of the powder to deionized water in step (2) is 1:10;

[0061] In step (2), the microwave power is 1000W, the extraction temperature is 95°C, and the extraction time is 4h; the centrifugation is performed at 800rpm for 15min;

[0062] The ultrafiltration treatment in step (3) has a molecular weight cut-off of 30,000 Da and a cut-off temperature of 45° C.

[0063] The volume ratio of the ultrafiltrate to deionized water in step (4) is 1:10;

[0064] The pH regulator in step (4) is a borate buffer.

[0065] Example 3

[0066] An eye drop for treating photochemical eye damage, comprising the following raw materials, measured by weight: 90 parts of polygonatum, 65 parts of musk, 65 parts of wild chrysanthemum, 52 parts of cassia seed, 52 parts of honeysuckle, 48 parts of phellodendron, 45 parts of houttuynia cordata, 25 parts of pearl powder, 15 parts of qinpi, 10 parts of complanatus, 9 parts of notopterygium wilfordii, and 4 parts of safflower yellow.

[0067] The preparation method of the above eye drops is the same as that of Example 1.

[0068] Comparative Example 1

[0069] An eye drop for treating photochemically damaged eyes is prepared by replacing 90 parts of polygonatum and 65 parts of musk with 155 parts of musk based on the raw materials of Example 3, while the rest of the ingredients are the same.

[0070] Comparative Example 2

[0071] An eye drop for treating photochemically damaged eyes is prepared by replacing 90 parts of polygonatum and 65 parts of musk with 155 parts of polygonatum, while the rest of the ingredients are the same as those in Example 3.

[0072] Comparative Example 3

[0073] An eye drop for treating photochemically damaged eyes is prepared by replacing 90 parts of polygonatum and 4 parts of safflower yellow with 94 parts of polygonatum based on the raw materials of Example 3.

[0074] Comparative Example 4

[0075] An eye drop for treating photochemically damaged eyes is prepared by replacing 4 parts of safflower yellow pigment with 4 parts of water based on the raw materials of Example 3, while the rest of the ingredients are the same.

[0076] Animal experiments

[0077] Healthy SD rats were selected and randomly divided into a blank group, a model group, Example 1 to 3 groups, and Comparative Example 1 to 4 groups, with 10 rats in each group. To establish an animal model of photochemical damage, a self-made light box (50×30×20) cm 3The four vertical surfaces and the top of the light box were sealed with reflective steel plates. Two white cold light lamps and an exhaust fan were installed inside the top of the box, and the bottom was a hollow iron frame. The average illuminance in all directions of the center of the light box was (10800±352) Lux, which was measured by a TES-1334A illuminometer, equivalent to 10000 Lux of each box wall at the eye level when the rats were normally active. All experimental rats were raised in an environment with alternating 12h light (300 Lux, 8:00-20:00) and 12h dark (5 Lux, 20:00-8:00), a temperature of 23-25℃, and a humidity of 55%-60%. They were dark-adapted for 12h before light exposure, and then placed in the light box for 24h continuous light exposure. Rats could move freely in the light box. The blank group was dark-adapted but no light exposure experiment was performed, and the detection time was the same as the other groups.

[0078] Each group of the embodiment and each group of the comparative example respectively corresponded to the eye drops prepared and administered by eye drops, and the model group and the blank group were administered with an equal amount of normal saline, twice a day, and the model was established after continuous administration for 7 days, and the administration was continued after the model was established. 7h and 7d after the model was established, the temporal half eyeball was taken, and after the normal saline was rinsed, it was put into the normal saline on ice, the corneoscleral limbus was cut open, the vitreous body and the anterior segment were removed, the eyeball wall was turned outward, and the retina was carefully peeled off under a dissecting microscope. After peeling, it was placed on an analytical balance and configured with 40g / L of wet weight with 0.86% normal saline, shaken left and right to be uniform, placed in a low-temperature centrifuge at 3000r / min for centrifugation for 15min, and the supernatant was taken. Malondialdehyde (MDA) and superoxide dismutase (SOD) were determined according to the method provided by the kit.

[0079] In principle, the retina is more susceptible to photooxidative damage due to its unique anatomical, physiological and biochemical characteristics. The outer segment of photoreceptor cells is rich in polyunsaturated fatty acids and is vulnerable to free radical attack. The inner segment is rich in mitochondria and has a high oxygen tension, so photoreceptor cells are vulnerable to free radical and lipid peroxide attack and damage. The main product of lipid peroxidation is malondialdehyde (MDA). By measuring the amount of MDA in the retina, the level of lipid peroxidation in the retina can be assessed, and it also reflects the severity of the cell being attacked by free radicals. Superoxide dismutase (SOD) is the main antioxidant enzyme in the body. It removes superoxide anion free radicals through dismutation reactions to reduce tissue damage, thereby playing a protective role in the process of retinal photochemical damage. Therefore, the level of SOD activity indirectly reflects the body's ability to remove oxygen free radicals. After ultraviolet light irradiation, the MDA content in rat skin, serum, lens and retina increased, and the SOD content decreased.

[0080] As can be seen from Table 1 and Table 2, the eye drops of the present invention can significantly improve the activity of SOD, reduce the generation of MDA, remove free radicals, reduce oxidative stress, and then prevent photoreceptor cell apoptosis and protect retinal structure. After the comparative example removes the key components, the protective effect is poor, indicating that the main raw materials, polygonatum and musk, have a synergistic effect, which is very critical for the formula to play the role of removing free radicals and reducing photochemical damage, and its dosage cannot be easily changed, and the protective effect is the result of multi-component synergistic compatibility. After the comparative example changes the amount of safflower yellow pigment or removes it, the therapeutic effect is significantly weakened, indicating that the use of safflower yellow pigment and a variety of medicinal materials can produce a synergistic effect and jointly improve the ability to protect photochemical damage.

[0081] Table 1 SOD activity in each group

[0082]

[0083]

[0084] Table 2 MDA content in each group

[0085]

[0086] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0087] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An eye drop for treating photochemically damaged eyes, characterized in that: The invention comprises the following raw materials: polygonatum, musk, wild chrysanthemum, cassia seed, honeysuckle, phellodendron, houttuynia cordata, pearl powder, qinpi, complanatus, notopterygium wilfordii, and safflower yellow pigment.

2. The eye drops for treating photochemically damaged eyes according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 80-100 parts of polygonatum, 60-70 parts of musk, 60-70 parts of wild chrysanthemum, 50-55 parts of cassia seed, 50-55 parts of honeysuckle, 45-50 parts of phellodendron, 40-50 parts of houttuynia cordata, 20-35 parts of pearl powder, 12-16 parts of qinpi, 8-12 parts of complanatus, 8-10 parts of notopterygium wilfordii and 2-5 parts of safflower yellow.

3. The eye drops for treating photochemically damaged eyes according to claim 2, characterized in that: The invention comprises the following raw materials in parts by weight: 90 parts of polygonatum, 65 parts of musk, 65 parts of wild chrysanthemum, 52 parts of cassia seed, 52 parts of honeysuckle, 48 parts of phellodendron, 45 parts of houttuynia cordata, 25 parts of pearl powder, 15 parts of qinpi, 10 parts of complanatus, 9 parts of notopterygium wilfordii and 4 parts of safflower yellow pigment.

4. A method for preparing an eye drop for treating photochemically damaged eyes according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) Weigh the raw materials according to the above weight parts and set aside; (2) adding polygonatum, musk, wild chrysanthemum, cassia seed, honeysuckle, phellodendron, houttuynia cordata, pearl powder, qinpi, complanatus, and notopterygium wilfordii into a ball mill and grinding the powder, soaking the obtained powder in deionized water, and then performing heating microwave-assisted extraction 2 to 3 times, combining the extracts, filtering, and centrifuging to obtain the supernatant; (3) subjecting the supernatant obtained in step (2) to ultrafiltration, and concentrating the obtained ultrafiltrate under reduced pressure to a relative density of 1.05 to 1.15; (4) Deionized water is added to the concentrated ultrafiltrate, and then safflower yellow pigment and a pH adjuster are added to adjust the pH to 6.5 to 7.5, and sterilized to obtain eye drops.

5. The method for preparing eye drops for treating photochemically damaged eyes according to claim 4, characterized in that: The average particle size of the powder in step (2) is 100-150 meshes, and the soaking time is 0.5-2 hours.

6. The method for preparing eye drops for treating photochemically damaged eyes according to claim 4, characterized in that: The mass ratio of the powder to deionized water in step (2) is 1:6-10.

7. The method for preparing eye drops for treating photochemically damaged eyes according to claim 4, characterized in that: In step (2), the microwave power is 800-1000W, the extraction temperature is 80-95°C, and the extraction time is 2-4h; the centrifugation is performed at 500-800rpm for 8-15min.

8. The method for preparing eye drops for treating photochemically damaged eyes according to claim 4, characterized in that: The molecular weight cut-off of the ultrafiltration treatment in step (3) is 30,000 Da, and the cut-off temperature is 30-45° C.

9. The method for preparing eye drops for treating photochemically damaged eyes according to claim 4, characterized in that: The volume ratio of the ultrafiltrate to deionized water in step (4) is 1:10-12.

10. The method for preparing eye drops for treating photochemically damaged eyes according to claim 4, characterized in that: The pH regulator in step (4) is selected from a phosphate buffer and a borate buffer.

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