39 results about "Tumour invasion" patented technology

The invention relates to the technical field of medical image processing, in particular to a multimodal-based digestive tract tumor lesion image segmentation method and segmentation system. The method comprises the following steps: acquiring an alimentary canal tumor lesion image, and extracting an alimentary canal tumor ultrasonic image; evaluating the tumor invasion depth based on the digestive tract tumor ultrasonic image; performing focus three-dimensional visual modeling according to the tumor invasion depth to obtain a digestive tract tumor focus model; extracting a tumor tissue pathological image according to the digestive tract tumor lesion image; performing tumor region segmentation based on the tumor tissue pathological image to obtain digestive tract tumor region data; performing tissue arrangement anomaly detection based on the digestive tract tumor region data to obtain tissue arrangement anomaly data; and calculating a tissue arrangement disorder index according to the tissue arrangement abnormal data and the digestive tract tumor area data. According to the invention, the tumor identification accuracy and the malignant region segmentation precision are improved based on the medical image processing technology.

The invention relates to a method for evaluating the specific killing ability of engineered natural killer cells. Specifically, the invention provides a method for dynamically detecting the killing ability of the immune effector cell in real time, a target cell capable of stably expressing fluorescent protein is constructed, and the real-time killing of the immune effector cell on the target cell is accurately reflected through the disappearance condition of a fluorescence signal of the target cell. In the system provided by the invention, target cells can be added again for continuous challenge, tumor invasion is simulated, and the in-vitro killing function of the natural killer cells of the chimeric antigen receptor is comprehensively evaluated. The method provided by the invention provides effective and accurate evaluation for process development of immune effector cells, especially engineered and modified NK cell products, and provides effective analysis support for researching immune curative effects of NK cells and the like.

The invention relates to a biomarker of a glioblastoma invasion leading edge region and application of the biomarker, and belongs to the technical field of biological detection. In order to solve the technical problems of unknown tumor invasion mechanism, scarcity of treatment targets and inaccurate prognosis evaluation caused by lack of a GBM invasion leading edge region specific marker in the prior art, 12 GBM invasion leading edge region specific high-expression genes, namely CREG2, KIF5A, CELF4, TMEM132D, KHDRBS2, SNAP25, SYT1, ASIC2, PAK3, RBFOX1, STMN2 and CNTNAP2, are analyzed and screened through space transcriptomics. A COX multi-factor regression risk assessment system is developed and constructed based on the markers. According to the invention, a molecular basis is provided for accurate recognition of a GBM invasion range, patient layering and prognosis prediction, targeted therapy development and curative effect monitoring.

The invention relates to a novel marker for a glioblastoma tumor transition region and application of the novel marker, and belongs to the technical field of biological detection. In order to solve the technical problems that in the prior art, tumor invasion leading edge markers cannot be effectively recognized, and postoperative residues and prognosis are difficult to accurately evaluate, 19 gene markers with high specificity expression in a tumor transition region, such as SELENOP, SHTN1 and CTNNA3, are screened out by combining space transcriptome analysis with a machine learning algorithm. The expression level of the marker is detected through digital imaging, nucleic acid sequencing and other technologies, a logistic regression prediction model with the AUC value larger than 0.95 is constructed, and the logistic regression prediction model is applied to diagnostic kit preparation, tumor layering screening and prognosis evaluation. According to the invention, precise recognition of the tumor transition region is realized, and a molecular basis is provided for postoperative recurrence monitoring, individualized treatment scheme making and novel treatment target screening.

Tumor budding (TB) is defined as a cluster of one to four tumor cells at the tumor invasive front. Though promising as a prognostic factor for colorectal cancer, its routine clinical use is hampered by high inter-and intra-observer disagreement on routine H&E staining. Therefore, automated methods are provided that minimize the inter-and intra-observer disagreement for tumor bud detection on images of H&E-stained tissue sections.

The present invention belongs to the field of biomedicine technology, and discloses the use of a quercetin derivative Q3MG in the preparation of anti-tumor invasion and metastasis drugs and drugs for targeted degradation of pro-IL-1β protein in cancer cells. The present invention first discovered that Q3MG can significantly inhibit tumor invasion and metastasis by targeted degradation of pro-IL-1β protein in cancer cells, and has a good therapeutic effect on anti-tumor invasion and metastasis, especially squamous cell carcinoma; it reveals the importance of quercetin derivatives in the preparation of drugs for the treatment of squamous cell carcinoma invasion and metastasis, and even the potential value of flavonoid compounds in various other tumors, which has far-reaching clinical significance and important prospects for promotion and application.

The invention relates to the field of biological medicine, and discloses application of GABRR1 as a tumor marker and a target molecule. According to the application disclosed by the invention, the GABRR1 is discovered as a marker of tumor invasion and metastasis for the first time and participates in growth and invasion and metastasis processes of tumors, so that the GABRR1 can be used as a target spot in diagnosis and / or treatment of the tumors; according to the invention, the GABRR1 protein is used as a tumor target for screening to obtain a monoclonal antibody, a polypeptide or a small molecule drug and other tumor treatment drugs capable of overexpressing GABRR1.

The present invention provides a TIPE3 immunohistochemical detection kit and a method and application thereof, belonging to the technical field of tumor detection. The present invention has found that TIPE3 expression is significantly increased in pancreatic cancer, is closely related to the invasion and metastasis potential of the tumor, and is an independent prognostic risk factor for pancreatic cancer patients. Therefore, the purpose of helping to judge the malignancy of pancreatic cancer and the prognosis of patients can be achieved by detecting the expression of TIPE3, thereby providing a TIPE3 immunohistochemical detection kit, which can effectively predict the malignancy and metastasis potential of pancreatic cancer, and perform recurrence and metastasis risk classification for pancreatic cancer patients; at the same time, the prognosis of patients can also be evaluated, thereby helping to improve the survival rate of pancreatic cancer patients, and providing an important scientific basis for the prevention and treatment of pancreatic cancer in clinical practice, so it has good clinical application value and social benefits.

The invention belongs to the technical field of biological medicine and tumor immunotherapy, and discloses application of 3-oxo-valproic acid coenzyme A in preparation of a medicine for inhibiting lung adenocarcinoma. As a specific agonist of GIMAP1, the 3-oxo-valproic acid coenzyme A is used for activating the GIMAP1, enhancing inhibition of lung cancer cell progress and enhancing activation of a tumor immune microenvironment, so that the lung adenocarcinoma is inhibited, and the lung adenocarcinoma is inhibited. The invention has the following beneficial effects: 1, strong targeting property: 3-oxo-valproate coenzyme A is specifically combined with GIMAP1, so that a mediated immunomodulatory pathway is accurately activated, and wide influence on normal cells is avoided; 2, immune microenvironment remodeling: the anti-tumor immune cell infiltration is remarkably improved, the lung adenocarcinoma immunosuppression microenvironment is reversed, and the immunotherapy sensitivity is improved; 3, tumor progression is inhibited, lung cancer cell proliferation is effectively inhibited, and the tumor invasion ability is reduced; 4, the source is natural: as an intestinal microbial metabolite derivative, the safety is high, and the side effect is lower than that of a chemically synthesized medicine.

The invention belongs to the technical field of biological medicines, and discloses application of a quercetin derivative Q3MG in preparation of drugs for resisting tumor invasion and metastasis and products for targeted degradation of pro-IL-1beta protein in cancer cells. It is found for the first time that Q3MG can significantly inhibit tumor invasion and metastasis through targeted degradation of pro-IL-1beta protein in cancer cells, and the Q3MG has a good treatment effect on tumor invasion and metastasis, especially squamous cell carcinoma; the invention reveals the importance of the quercetin derivative in preparation of drugs for treating squamous cell carcinoma invasion and metastasis, even the potential value of flavonoid compounds in other various tumors, and has profound clinical significance and important popularization and application prospects.

The present invention relates to the field of biomedicine and discloses the application of GABRR1 as a tumor marker and a target molecule. The present invention firstly discovers that GABRR1 serves as a marker for tumor invasion and metastasis and participates in the processes of tumor growth, invasion and metastasis. Therefore, GABRR1 can be used as a target in the diagnosis and / or treatment of tumors; the present invention uses the GABRR1 protein as a tumor target to screen tumor therapeutic drugs such as monoclonal antibodies, polypeptides or small molecule drugs that can overexpress GABRR1.

The invention discloses a method for regulating and controlling tumor invasion and migration ability through a TGF-betaSmad7 signal by using MYL9 as a marker, which is characterized by comprising the following steps: (1) culturing kidney cancer cells in a DMEM (Dulbecco Modified Eagle Medium) added with 10% fetal calf serum; (2) carrying out gene silencing by using a HiPerFect transfection reagent, specifically knocking down the expression of MYL9, and regulating and controlling a TGF-beta1 / Smad7 signal channel; and (3) detecting the migration and invasion ability of the kidney cancer cells by adopting a Transwell experiment, adding 200 mu L of DMEM containing 0.5% of FBS into the upper hole, adding 600 mu L of DMEM containing 10% of FBS into the lower hole, and filling the kidney cancer cells treated in the step (2) for a migration and invasion experiment. MYL9 is connected with matrix mechanics, angiogenesis and immune escape through a TGF-beta / Smad7 signal axis, and by regulating and controlling the TGF-beta Smad7 signal, the invasion ability of kidney cancer cells can be effectively reduced, the traditional cognition of single-dimensional regulation and control on a kidney cancer microenvironment is broken through, and a multi-dimensional perspective is provided for understanding tumor progression.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to human serum albumin nanoparticles entrapped with alpha-mangostin as well as a preparation method and application of the human serum albumin nanoparticles. The alpha-M entrapped human serum albumin nanoparticles provided by the invention comprise an alpha-M raw material medicine and human serum albumin (HSA), the preparation method comprises the following four steps: high-pressure homogenization, rotary evaporation, degerming filtration and freeze drying. The nano delivery system has good safety and stability, and is proved to have multiple effects of immunoregulation, tumor invasion and metastasis inhibition, matrix barrier weakening and the like at cellular level and animal level; the compound can be used for preparing anti-tumor drugs including pancreatic cancer, breast cancer, colon cancer and the like, can be used for effectively inhibiting tumor progression when being combined with chemotherapy drugs, and has broad-spectrum anti-cancer application value.

The invention provides a lung cancer cell LLC-ML2 as well as an establishment method and application thereof. The lung cancer cells are used for highly expressing CD18 molecules, Strc molecules, Cd79a molecules, Amigo2 molecules, Plet1 molecules, Angptl4 molecules and Plac1 molecules; ddx3y, Cbr3, Sox11, Pid1, Kdm5d, Eif2s3y and Capn6 molecules are not expressed or are subjected to low expression. The LLC cell strain is obtained by inoculating common LLC cells to a C57BL / 6 mouse, taking a lung metastatic focus for culture, inoculating primarily cultured metastatic focus cells to the C57BL / 6 again, taking the lung metastatic focus for different clone culture, and screening. Through two times of lung metastatic focus tumorigenesis and screening, compared with LLC cells, the lung cancer cells are shorter in use time for constructing a metastasis model, and the metastasis rate of main organs is higher; an efficient cell model is provided for better researching a tumor invasion and metastasis mechanism.

The invention relates to an in-vitro tumor cell 3D budding invasion model and a construction method and application thereof. A chip model for researching tumor invasion behaviors is manufactured through a microlithography technology. The model can simulate the interaction between a tumor cell nest and an induced cell in an in-vivo tumor microenvironment. The model has the advantage that the influence and promotion effect of the induced cells on the invasion behavior of the tumor cell nests can be intuitively monitored in real time. Compared with the prior art, the 3D invasion model has remarkable advantages in the aspects of universality and practicability. The device is not only suitable for research of different types of tumors, but also capable of displaying the dynamic invasion process of tumor cell nests in different forms under the action of induced cells. The model provides important experimental basis and theoretical support for research on tumor invasion behaviors, and has a good application prospect.

This invention belongs to the field of biomedical technology, specifically disclosing an injectable embolic agent, its preparation method, and its application. The embolic agent is a hydrocolloid system containing core-shell structured nanoparticles: the core contains Cu... 2+ Salt and ATOX1 inhibitors, where ATOX1 inhibitors can inhibit copper ion efflux and promote intracellular copper accumulation, and interact with Cu 2+ It synergistically induces copper death in tumor cells; the shell is a thermosensitive block copolymer hydrogel that can interact with Cu. 2+ Coordination bonds are formed, enhancing the stability of nanoparticles, while a sol-gel transition occurs at physiological temperatures, enabling precise vascular embolization; the overall core-shell structure allows for the formation of Cu... 2+ The sustained-release effect of ATOX1 inhibitors was observed. In the VX2 rabbit hepatocellular carcinoma model, this embolization agent significantly improved tumor necrosis rate and reduced metastasis rate compared to traditional iodized oil embolization; it also downregulated hypoxia- and angiogenesis-related factors such as HIF-1α, VEGF, and CD31, inhibited MMP9-mediated tumor invasion and metastasis, and promoted CD8+. + T-cell infiltration effectively improves the tumor immunosuppressive microenvironment after transarterial chemoembolization.

This invention relates to the field of surgical navigation technology for tumor treatment, specifically to an intelligent navigation system for nasal skull base tumor surgery based on multimodal image fusion. The system includes an image acquisition module for acquiring and rigidly registering CT and MRI images to determine the initial tumor region; a tumor invasion degree acquisition module for quantifying the local tumor invasion degree by combining CT grayscale distribution characteristics and MRI apparent diffusion coefficient characteristics; an invasion path acquisition module for simulating a fully covered digital invasion path based on anisotropic diffusion of image features; a risk infiltration degree acquisition module for comprehensively considering distance, direction, and invasive activity to assess the risk of invasion path infiltrating key structures; and a tumor boundary acquisition module for obtaining the tumor biological boundary by correcting the anatomical boundary through risk infiltration degree. This invention effectively improves the accuracy and safety of nasal skull base tumor surgery by acquiring the tumor biological boundary.

The invention relates to the technical field of tumor cell statistics. The tumor cell statistical method, device, equipment and medium are provided, and the method comprises the following steps: based on mark information in a digital pathological image, performing tumor core region segmentation and invasion leading edge boundary identification processing, and generating tumor invasion leading edge boundary coordinates; based on the tumor infringement leading edge boundary coordinates and the marking information, performing multi-dimensional quantification processing on tumor cell space distribution characteristics to generate infringement mode characteristic vectors; performing multi-dimensional weighted aggregation processing on the invasion mode feature vector to generate a tumor invasion ability quantitative index; and performing comprehensive evaluation report generation processing based on the tumor invasion capability quantitative index to obtain a quantitative evaluation report so as to improve the spatial heterogeneity characterization capability, enhance the multi-scale spatial modeling precision, optimize the clinical interpretability of the quantitative index and realize the integration of structured report output.

The present invention discloses a method for rapid design of fluorescent probes for preliminary screening indicators of tumors. First, zinc finger proteins, biomarkers related to tumor invasion and migration, are selected. Quinoline is selected as the parent structure based on its structural characteristics. Then, the zinc finger proteins are transformed using the concept of combinatorial chemistry to obtain a reasonable probe candidate library. Then, a gradient optimized conformation search algorithm is used to specify a zinc finger protein, and the binding effect between the zinc finger protein and various candidates is scored and evaluated to find the structure with the highest score. The spectral properties are preliminarily verified. Finally, by comparing the serum test results of breast cancer patients and healthy people, it is proved that the fluorescent probe has a good signal response to the sample, and the application potential of the total amount of zinc finger proteins as a preliminary screening indicator for tumors is explored. The method in the present invention can accurately and efficiently design fluorescent probes for exploring preliminary screening indicators for tumors, providing a new idea for realizing rapid screening of tumors.

The invention relates to the field of medicine, in particular to a method for culturing an in-vitro tumor invasion evaluation model, the model obtained through the method and a medicine screening method. The method comprises the following steps: constructing a first cell model with high cell density and / or low cell density; the first cell model is cultured under the condition of high osmotic pressure and / or low osmotic pressure, the low osmotic pressure condition is a low osmotic medium composed of a complete medium and water according to the proportion of 3: 1 to 2: 1, and the high osmotic pressure condition is a high osmotic medium composed of a complete medium and a sucrose solution according to the proportion of 4: 1 to 3: 1. According to the method, the synergistic effect of solid stress and intracellular hydrostatic pressure is considered, the tumor invasion evaluation model and method based on the intracellular and intracellular pressure difference are provided, and an in-vitro model capable of truly and effectively simulating in-vivo tumor invasion can be provided.

The present invention relates to a class of multi-target fusion proteins for anti-tumor invasion and metastasis, and a preparation method and application thereof. The fusion protein has the following amino acid sequence structure: RGD peptide - linker peptide - TRAIL functional peptide segment - linker peptide - NGR peptide; the fusion protein simultaneously has the anti-tumor cell proliferation effect of the TRAIL peptide segment and the tumor cell migration inhibitory effects of two targeting peptides, RGD and NGR; through comparison, it is found that the multi-target fusion protein has more significant anti-tumor activity, reverses the drug resistance of TRAIL to a certain extent, and can be used for preparing anti-tumor drugs.

The invention provides a bone and soft tissue solid tumor three-dimensional model as well as a construction method, application, a detection method and a culture reagent combination thereof, and belongs to the technical field of bioengineering. The construction method comprises the following steps: acquiring solid tumor cells of bone and soft tissue tumors; inoculating the solid tumor cells into a culture container, adding a solid tumor two-dimensional cell culture medium, and performing two-dimensional culture; the method comprises the following steps: mixing solid tumor cells subjected to two-dimensional culture with vascular endothelial cells according to a certain cell proportion, carrying out balling culture to form a globule polymer, adding an extracellular matrix into the globule polymer, gelling, adding into a solid tumor three-dimensional cell culture medium, and culturing to form a bone and soft tissue solid tumor three-dimensional model with a vascular structure. According to the construction method, 2D culture is carried out firstly, then 3D balling culture is carried out, the survival rate is effectively increased, and the formed three-dimensional model has a tumor microenvironment formed by a vascular structure and can be used for drug detection and tumor invasion assessment analysis.

The present invention discloses a mixed epithelial-mesenchymal phenotype tumor cell model based on the bidirectional regulatory function of keratin5, belonging to the technical field of tumor models. The model is a stable mixed E / M phenotype cell model obtained by knocking down the keratin5 gene in epithelial cells. The present invention also discloses a method for constructing the above model. The mixed E / M phenotype cells in the cell model of the present invention have significantly enhanced stemness, in vitro migration and invasion capabilities, and resistance to the chemotherapy drug cisplatin, thereby facilitating the study of tumor invasion and metastasis.

The present disclosure relates to the use of compounds such as compounds of Formula (I) or a pharmaceutically acceptable salt, solvate and / or prodrug thereof in treating cancer and / or reduction and / or prevention of tumor invasion and / or metastasis.

This invention relates to the fields of bionanotechnology and molecular imaging, disclosing a probe, its preparation method, and its applications. The probe comprises, from the inside out, a near-infrared II (NIR-II) fluorescent core, a gold nanoshell, an MMP-responsive peptide layer, and a pH-sensitive polymer layer. Utilizing the quenching effect of the gold shell on the core fluorescence, the probe remains in a "silent" state in the circulatory system. Its core design lies in the physical shielding effect of the peptide layer, ensuring that the probe must first be unlocked by cleavage by MMP enzymes highly expressed in the tumor, followed by protonation and shedding of the polymer layer under a slightly acidic environment to restore fluorescence. This invention effectively overcomes the non-specific activation of single-response probes in inflammatory or necrotic areas, significantly improves the imaging contrast at the tumor invasion front, and provides a highly sensitive and deeply penetrating visual navigation method for precise surgical resection of tumors.

The application provides lung cancer cells and a method for establishing the same and application. The lung cancer cells highly express CD18, Strc, Cd79a, Amigo2, Plet1, Angptl4 and Plac1 molecules; do not express or lowly express Ddx3y, Cbr3, Sox11, Pid1, Kdm5d, Eif2s3y and Capn6 molecules. The lung cancer cells are obtained by inoculating common LLC cells into C57BL / 6 mice, culturing lung metastatic foci, inoculating the primary cultured metastatic foci cells into C57BL / 6 again, culturing different clones of lung metastatic foci again, and screening. After twice lung metastatic foci tumorigenesis and screening, compared with LLC cells, the lung cancer cells are shorter in use time for constructing a metastasis model, and higher in metastasis rate of main organs; and the lung cancer cells provide a high-efficiency cell model for better studying the mechanism of tumor invasion and metastasis.

The present invention relates to the field of medical image processing technology, and in particular to a multimodal digestive tract tumor lesion image segmentation method and segmentation system. The method comprises the following steps: acquiring a digestive tract tumor lesion image and extracting a digestive tract tumor ultrasound image; assessing the tumor invasion depth based on the digestive tract tumor ultrasound image; performing three-dimensional visualization modeling of the lesion according to the tumor invasion depth to obtain a digestive tract tumor lesion model; extracting a tumor tissue pathology image based on the digestive tract tumor lesion image; performing tumor region segmentation based on the tumor tissue pathology image to obtain digestive tract tumor region data; performing tissue arrangement abnormality detection based on the digestive tract tumor region data to obtain tissue arrangement abnormality data; and calculating a tissue arrangement disorder index based on the tissue arrangement abnormality data and the digestive tract tumor region data. The present invention improves the accuracy of tumor recognition and the accuracy of malignant region segmentation based on medical image processing technology.

The invention relates to a preparation method and device of a high-throughput suspension cell sphere assembly, and belongs to the technical field of biological cell three-dimensional culture. In order to solve the problems that an existing three-dimensional cell sphere assembly technology is complex in operation, low in flux, depends on external field guidance and has large interference on cell activity, the invention provides a method for realizing non-contact capture, transfer and space collection of cell spheres by utilizing the physical characteristics that the density of suspension cell spheres is lower than that of a culture solution and the suspension cell spheres stably float to the top ends of liquid drops. And finally, fusing to form the three-dimensional cell assembly. The device is a horizontal assembling mechanism or a vertical assembling mechanism, the horizontal assembling mechanism achieves pairing of spheres in the horizontal direction through liquid drop sandwich transfer formed by a first substrate, a second substrate and a spacer, and the vertical assembling mechanism achieves stacking of the spheres in the vertical direction through a capillary tube and a positioner under the capillary action. The three-dimensional co-culture system is used for constructing a high-throughput drug screening model, tumor invasion and microenvironment interaction research and tissue engineering related three-dimensional co-culture system.

This invention discloses a polypeptide combination for inhibiting tumor invasion and metastasis, belonging to the field of therapeutic technology for metastatic breast cancer. The polypeptide combination comprises two polypeptides, each containing a targeting peptide P1 and a targeting peptide P2, respectively. A membrane-penetrating peptide is attached to the N-terminus of both targeting peptides P1 and P2. Histone-lysine N-methyltransferase EZH2 preferentially methylates the aforementioned polypeptide combination, thereby reducing the methylation of lysine residues at positions 53 and 333 of the SMAD3 protein, thus inhibiting tumor cell invasion and metastasis.

The invention belongs to the technical field of biology, discloses application of targeted LRTOR in development of drugs for preventing and / or treating osimertinib drug resistance and invasion and metastasis of non-small cell lung cancer, and particularly discloses application of an LncRNA LRTOR inhibitor in promoting sensitivity of tumors to osimertinib. The invention discloses that LncRNA LRTOR plays a crucial role in NSCLC osimertinib drug resistance for the first time, and can be used as a target for preventing and / or treating NSCLC osimertinib drug resistance and transfer. Targeted inhibition of LncRNA LRTOR can greatly enhance the sensitivity of NSCLC to osimertinib treatment, significantly inhibit the activity and proliferation of osimertinib drug-resistant cells, significantly inhibit the invasion and metastasis capacities and cell dryness of drug-resistant tumors, and inhibit the growth of osimertinib drug-resistant tumors.