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A detection, treatment and prognosis target and application of cholangiocarcinoma

A cholangiocarcinoma-specific technology, applied in the field of biomedicine, can solve the problems of insensitivity of cholangiocarcinoma to chemotherapy and difficulty in entering the anatomical location of cholangiocarcinoma

Active Publication Date: 2020-09-01
THE SECOND HOSPITAL OF SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, access to the anatomical location of cholangiocarcinoma is difficult, which poses a great challenge to the treatment of cholangiocarcinoma
Surgical treatment of cholangiocarcinoma is the treatment of choice, but it is only effective in the early stages of the disease, and cholangiocarcinoma is not sensitive to chemotherapy

Method used

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  • A detection, treatment and prognosis target and application of cholangiocarcinoma
  • A detection, treatment and prognosis target and application of cholangiocarcinoma
  • A detection, treatment and prognosis target and application of cholangiocarcinoma

Examples

Experimental program
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Embodiment 1

[0022] Cell Culture and Transfection

[0023] Human cholangiocarcinoma cell lines QBC939 and RBE obtained from the Chinese Academy of Sciences (Shanghai) type culture cell bank were used at 37°C, 5% CO 2 1640 medium (Hyclone, Logan, UT, USA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA) and penicillin / streptomycin. After entering the logarithmic growth phase, the cells were washed 3 times with PBS, digested with trypsin, and placed in 6-well plates. When the cell density of the 6-well plate reached 50-60% confluency, Lipofectamine 2000 was used to transfect siRNA (Invitrogen, USA). siRNA targeting USP8 (si-USP8) was synthesized by OriGene (Beijing, China), and the siRNA negative control (OriGene) was used as a negative control (si-NC).

[0024] qRT-PCR analysis

[0025] 24 hours after transfection with siRNA, total RNA was extracted from the cells with Ultrapure RNA Kit (CWBIO, Beijing, China), and reverse transcribed into cDNA with HiFiScript cDNA Synt...

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Abstract

The invention relates to a cholangiocarcinoma cell medicine target point. The medicine target point is ubiquitin specific protease 8, and the nucleotide sequence is shown as SEQID NO:1 in a sequence table. For the first time, the invention puts forward that USP8 is knocked down, so that proliferation, migration and invasion of cholangiocarcinoma cells can be notably restrained, MMP9 activity achieving important effects on cell adhesion, neoplasm invasiveness and transfer is reduced, and the USP8 is promoted to achieve effect of oncogene for growth and transfer ability of cholangiocarcinoma. New possibility is provided for development of cholangiocarcinoma medicines and cholangiocarcinoma prevention, treatment and prognosis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a detection, treatment and prognosis target of cholangiocarcinoma and its application. Background technique [0002] Cholangiocarcinoma is the second most common primary hepatobiliary tumor with a high incidence and recurrence rate. Globally, cholangiocarcinoma is the second most common primary hepatobiliary tumor after hepatocellular carcinoma, accounting for 10%-15% of the incidence of hepatobiliary tumors. It has been reported that the incidence of total cholangiocarcinoma has gradually increased worldwide in recent decades, especially in Southeast Asia where the incidence is highest. Cholangiocarcinoma is a malignant tumor with no obvious clinical manifestations in the early stage. Furthermore, it is difficult to access the anatomical location of cholangiocarcinoma, which poses a great challenge to the treatment of cholangiocarcinoma. Surgery for cholangiocarcinoma is th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N9/50A61K31/7105A61P35/00
CPCA61K31/7105A61P35/00C12N9/50C12Q1/6886C12Q2600/158
Inventor 靖旭武静郑惠延王道清
Owner THE SECOND HOSPITAL OF SHANDONG UNIV
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