Impedance sensing method for monitoring population cell invasion in three-dimensional matrix

Abstract

The invention provides an impedance sensing method for monitoring population cell invasion in a three-dimensional matrix. The method comprises the following steps: S1, preparing a conductive chip with a microelectrode array; s2, surrounding the periphery of the conductive chip with the annular structure to form a cell culture cavity; s3, performing surface treatment on the conductive chip; s4, fully paving cells on the microelectrode array of the cell culture cavity and forming polymeric hydrogel; s5, performing cell impedance detection in real time to obtain impedance information of the cells so as to obtain information of a cell invasion process. According to the invention, collagen I type gel suitable for a tumor invasion model is prepared on a microelectrode array conductive chip fully paved with cells through a self-assembly fibrosis process, and efficient and stable charge transfer in a cell impedance sensing detection process is effectively realized in combination with an impedance spectrometer on-line detection system; therefore, the technical effects of real-time, quantitative, label-free and whole-course dynamic analysis are achieved for detecting the invasion of the population cells in the three-dimensional matrix.

Description

technical field [0001] The present disclosure relates to the technical field of cell impedance sensing detection, in particular to an impedance sensing method for monitoring colony cell invasion in a three-dimensional matrix. Background technique [0002] Tumors are characterized by cellular heterogeneity, and the major contributor to tumor lethality is cancer spread. The process of metastasis enables tumor cells to break away from the primary tumor and seed at distant sites in the body. The ability of cells to migrate and degrade the extracellular matrix (ECM) is a key requirement for invasion. By developing in vitro three-dimensional (3D) analysis techniques to distinguish more invasive or migratory phenotypes, it is possible to evaluate drug efficacy. Potential indicators for valence, routine biochemical and immunological testing. Population migration of cells is often studied in two-dimensional (2D) monolayers, since biochemical and physical parameters of the ECM, such...

AI Technical Summary

Problems solved by technology

[0005] In view of the above problems, the present disclosure provides an impedance sensing method for monitoring population cell invasion in a three-dimensional matrix, which is used to at least partially solve the endpoint summation of the traditional Boyden chamber experiment limited to the number of cells on the lower side of the membrane, thereby affecting histology. Technical Issues for a Comprehensive Assessment of Cell Invasion Mechanisms in 3D

Images