Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cholangiocarcinoma detection, treatment and prognosis target point and application

A technique for cholangiocarcinoma and bile duct, which is applied in the field of biomedicine and can solve the problems of insensitivity to chemotherapy of cholangiocarcinoma and difficulty in entering the anatomical position of cholangiocarcinoma.

Active Publication Date: 2020-01-17
THE SECOND HOSPITAL OF SHANDONG UNIV
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, access to the anatomical location of cholangiocarcinoma is difficult, which poses a great challenge to the treatment of cholangiocarcinoma
Surgical treatment of cholangiocarcinoma is the treatment of choice, but it is only effective in the early stages of the disease, and cholangiocarcinoma is not sensitive to chemotherapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cholangiocarcinoma detection, treatment and prognosis target point and application
  • Cholangiocarcinoma detection, treatment and prognosis target point and application
  • Cholangiocarcinoma detection, treatment and prognosis target point and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Cell Culture and Transfection

[0023] Using the human cholangiocarcinoma cell lines QBC939 and RBE obtained from the Chinese Academy of Sciences (Shanghai) type culture cell bank, at 37 °C, 5% CO 2 1640 medium (Hyclone, Logan, UT, USA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA) and penicillin / streptomycin. After entering the logarithmic growth phase, the cells were washed 3 times with PBS, digested with trypsin, and placed in 6-well plates. When the cell density of the 6-well plate reached 50-60% confluence, Lipofectamine 2000 was used to transfect siRNA (Invitrogen, USA). siRNA targeting USP8 (si-USP8) was synthesized by OriGene (Beijing, China), and the siRNA negative control (OriGene) was used as a negative control (si-NC).

[0024] qRT-PCR analysis

[0025] 24 hours after transfection with siRNA, total RNA was extracted from the cells with the Ultrapure RNA Kit (CWBIO, Beijing, China), and reverse-transcribed into cDNA with the HiFiScript...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a cholangiocarcinoma cell medicine target point. The medicine target point is ubiquitin specific protease 8, and the nucleotide sequence is shown as SEQID NO:1 in a sequence table. For the first time, the invention puts forward that USP8 is knocked down, so that proliferation, migration and invasion of cholangiocarcinoma cells can be notably restrained, MMP9 activity achieving important effects on cell adhesion, neoplasm invasiveness and transfer is reduced, and the USP8 is promoted to achieve effect of oncogene for growth and transfer ability of cholangiocarcinoma. New possibility is provided for development of cholangiocarcinoma medicines and cholangiocarcinoma prevention, treatment and prognosis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a detection, treatment and prognosis target of cholangiocarcinoma and its application. Background technique [0002] Cholangiocarcinoma is the second most common primary hepatobiliary tumor with a high incidence and recurrence rate. Globally, cholangiocarcinoma is the second most common primary hepatobiliary tumor after hepatocellular carcinoma, accounting for 10%-15% of the incidence of hepatobiliary tumors. It has been reported that the incidence of total cholangiocarcinoma has gradually increased worldwide in recent decades, especially in Southeast Asia where the incidence is highest. Cholangiocarcinoma is a malignant tumor with no obvious clinical manifestations in the early stage. Furthermore, it is difficult to access the anatomical location of cholangiocarcinoma, which poses a great challenge to the treatment of cholangiocarcinoma. Surgery for cholangiocarcinoma is th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N9/50A61K31/7105A61P35/00
CPCA61K31/7105A61P35/00C12N9/50C12Q1/6886C12Q2600/158
Inventor 靖旭武静郑惠延王道清
Owner THE SECOND HOSPITAL OF SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com