Application of valproic acid in preparation of multidrug resistance reversal agent of lung cancer resistance medicine

A technology for anti-lung cancer drugs and multi-drug resistance, which is applied in drug combinations, anti-tumor drugs, active ingredients of anhydride/acid/halide, etc., and can solve problems such as the harm of tumor drug resistance and the enhancement of migration and erosion capabilities

Inactive Publication Date: 2013-06-12
SOUTHERN MEDICAL UNIVERSITY
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Problems solved by technology

We and Anthony found that after lung cancer cells and colorectal cancer cells become resistant to platinum drugs, the cells will undergo epithelial-mesenchymal transformation (EMT), and their ability to migrate and erode is significantly enhanced compared with t

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  • Application of valproic acid in preparation of multidrug resistance reversal agent of lung cancer resistance medicine
  • Application of valproic acid in preparation of multidrug resistance reversal agent of lung cancer resistance medicine
  • Application of valproic acid in preparation of multidrug resistance reversal agent of lung cancer resistance medicine

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with specific experiments, but not limited thereto.

[0028] Valproic acid (VPA) and cisplatin used in the experiment were purchased from Sigma.

[0029] 1. Inhibitory effect of valproic acid on the proliferation of cisplatin-resistant strains of human lung cancer:

[0030] 1. MTT method was used to detect the proliferation inhibitory effect of different doses of VPA on A549 / DDP after 24 hours of action:

[0031] Take the logarithmic growth phase cells of human lung cancer cisplatin-resistant strain (A549 / DDP) (cisplatin IC 50 =35 μmol, the multiplier of drug resistance is about 6 times, Department of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University), the adjusted cell number is 3×10 3 cells / well, inoculated into 96-well cell culture plate, added complete medium (RPMI-1640+10% fetal bovine serum, the same below), 37°C 5% CO 2 Cultivate in the incubator for 24 hours, re...

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Abstract

The invention discloses application of valproic acid in preparation of a multidrug resistance reversal agent of a lung cancer resistance medicine, and further discloses application of the valproic acid in the preparation of chemotherapeutics sensitizer of lung cancer resistance and a multidrug resistance tumor invasion resisting and transferring medicine. The application of the valproic acid in the preparation of the multidrug resistance reserval agent of the lung cancer resistance medicine verifies that valproic acid has significant function of a cisplatin persister of human lung cancer resistance through experiments, and verifies that the valproic acid can significantly change drug tolerance of a human lung cancer cell to the cisplatin, improve sensitivity of the human lung cancer cell to the cisplatin, and have obvious restraining effect of invasion effect of the persister of the lung cancer cisplatin.

Description

technical field [0001] The invention relates to the application of valproic acid in the preparation of anti-lung cancer drug multidrug resistance reversal agent. Background technique [0002] According to the "Third National Cause of Death Investigation" report released by the Ministry of Health in April 2008, lung cancer has replaced liver cancer as the number one cause of death from malignant tumors in my country (accounting for 22.7% of all malignant tumor deaths). Lung cancer often has no obvious symptoms in the early stage, especially non-small cell lung cancer (NSCLC), which accounts for 80% of lung cancers. 65-80% of patients are diagnosed in the middle and late stage when they are diagnosed, with distant metastasis, and often lose the opportunity of surgery and radiotherapy; therefore In the treatment of lung cancer, the role of drugs is particularly important. Unfortunately, there is still a lack of long-lasting and effective drugs for the drug treatment of lung ca...

Claims

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Application Information

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IPC IPC(8): A61K33/24A61K31/19A61P35/00A61P11/00
Inventor 饶进军陈云燕王新波吴少瑜吕琳王文雅杨迎暴
Owner SOUTHERN MEDICAL UNIVERSITY
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