76results about How to "Ability to inhibit metastasis" patented technology

The invention provides whitening essence and a preparation method thereof. The whitening essence consists of the following components of glycerine, 1,3-butanediol, sodium hyaluronate, EDTA-2Na, polyglycerol-10, sodium polyacrylate, xanthan gum, hydroxyacetophenone, dipotassium glycyrrhizinate, hydrolyzed sclerotium gum, gluconic acid sodium salt, glycereth-26, caprylhydroxamic acid, glycerol caprylate, 3-o-ethyl ascorbic acid, tranexamic acid, ceramide, phenethyl resorcinol, diglucosyl gallic acid and water. The whitening essence disclosed by the invention can refrain conversion of tyrosine tomelanin before the tyrosine is in preparation of conversion to the melanin, so that the activity of the tyrosinase is also high, generation of more melanin cannot be caused, and transfer of skin melanin can also be restrained. Besides, the whitening essence has powerful antioxidant ability, has light protection effects on skin, can control dermatitis, and is a whitening product most comprehensivein action mechanism at present, so that whitening effects can be achieved.

The invention discloses a reversibly crosslinked biodegradable polymersome with an asymmetric membrane structure as well as a preparation method and application thereof to nucleic acid medicines. The polymersome and the preparation method have the advantages that a triblock polymer PEG-P(TMC-DTC)-PEI or PEG-P(DLLA-DTC)-PEI is synthesized and is self-assembled and self-crosslinked to obtain the polymersome with the asymmetric membrane structure or the triblock polymer is linked with a targeting molecule and is self-assembled to obtain targeting RCCPs; the inner shell of the polymersome is PEI and is used for compounding and loading nucleic acid through electrostatic interaction; a membrane is reversibly crosslinked biodegradable polyester/polycarbonate with good biocompatibility; dithiolane of a side chain is similar to a human body natural antioxidant lipoic acid; the outer shell of the polymersome takes PEG as the background and can target cancer cells; by studying that the carrier is compounded with functional siRNA and studying the gene silencing effects in vitro and vivo, in vivo blood circulation and bio-distribution, the condition of treating lung cancer in situ bearing mice and toxic and side effects of the polymersome, the polymersome is expected to become a nanosystem platform integrating the advantages of simplicity, stability, multifunction, and the like and is used for carrying out efficient and active targeting delivery on siRNA to tumors in situ.

The invention discloses application of a long-chain non-coding RNA (Ribose Nucleic Acid) gene LOC553103 to the preparation of nasopharyngeal darcinoma cell inhibitors. The RNA interference sequence of the LOC553103 is used for preparing a preparation for inhibiting the growth and the proliferation of nasopharyngeal darcinoma cells and preventing the tumor cell invasion migration, and is further used for preparing a preparation for inhibiting the framework assembly and reconstruction of the nasopharyngeal darcinoma cells. A powerful molecular biology tool is provided for the auxiliary treatment of the nasopharyngeal darcinoma, and profound clinical significance and important popularization and application prospects are realized.

The invention discloses application of siRNA of long non-coding RNA LINC00673 and an inhibition preparation. The sequence of the siRNA of a targeted LINC00673 is designed, synthesized, and transfected into oral cancer cell strains. It is proved that the expression of the targeted interference LINC00673 can obviously inhibit the invasion and transfer abilities of the oral cancer cells. The inhibition preparation provides a new and potential pathway for treating an oral cancer.

The invention discloses application of etomoxir to treatment of colorectal cancer and particularly relates to application of etomoxir to preparation of medicines for preventing and treating colorectalcancer, especially medicines for inhibiting colorectal cancer metastasis. The research shows that etomoxir is capable of obviously inhibiting colony formation under anoikis of colorectal cancer cells, accelerating anoikis of colorectal cancer cells and inhibiting metastasis capability of the colorectal cancer cells in the body, has an obvious effect of treating the colorectal cancer and especially has an obvious effect of inhibiting colorectal cancer metastasis, so that etomoxir can be used for treating colorectal cancer metastasi. The invention provides novel application of etomoxir, expandsthe clinical application fields of etomoxir and further provides a novel manner for treating colorectal cancer metastas; etomoxir has good popularization and application prospects.

The invention discloses application of a long-chain non-coding RNA gene LOC553103 in preparation of a gastric cancer cell inhibitor. An RNA interference sequence of the LOC553103 is used for preparing a preparation for inhibiting growth and proliferation of gastric cancer cells and preventing invasion and transfer of the cancer cells, and further used for preparing a preparation for inhibiting assembly and reconstruction of a gastric cancer cell skeleton. A powerful molecular biology tool is provided for assisted treatment of gastric cancer, and the application has far-reaching clinical significance and important popularization and application prospect.

The invention belongs to the field of nanomedicine and biomedicine, and discloses a preparation method and application of highly nucleus-targeted anti-tumor nanomedicine. According to the preparationmethod and the application of the highly nucleus-targeted anti-tumor nanomedicine, a polypeptide with nucleus targeting ability and graphite powder are subjected to blending and ball milling, and polypeptide functionalized graphene (TG) with high tumor targeting ability and nucleus targeting ability is prepared under normal temperature and pressure. Furthermore, carboxyl group is introduced into the TG by using an acetic acid plasma technology, and the anti-tumor drug mitomycin C (MMC) is loaded on the TG. The prepared anti-tumor nanomedicine MMC-TG has good tumor cell nucleus targeting ability and can highly selectively target tumor cells. The tumor killing rate is 95% or above, but at the same time, the anti-tumor nanomedicine MMC-TG has little damage to normal cells. Experimental results confirm that the developed nanomedicine first plays a role in the nucleus, and has efficient tumor cell nucleus targeting ability, tumor killing ability and tumor metastasis inhibiting ability.

The invention provides interfering RNA and a lentivirus vector targeted to the OLFM4 gene and application of the interfering RNA and the lentivirus vector in preparation of drugs used for preventing and treating metastasis of stomach cancer tumors to the abdominal cavity or/and the liver. According to the invention, since the interfering RNA can inhibit in vitro migration and invasion ability of gastric cancer cells and liver metastasis capability of gastric cancer cells, decreased expression of OLFM4 is a potential effective mode for treatment of malignant progression of late gastric cancer. It is further determined that an NF-kB signal is an upstream regulation transcription factor of expression of OLFM4 and the interfering RNA has NF-kB signal feeding back and regulating functions.

The invention belongs to the technical field of biology, and relates to application of a reagent for inhibiting an SGCE gene and a tumor chemotherapy drug to preparation of a drug for treating tumor chemotherapy and application of a reagent for inhibiting the SGCE gene to preparation of a drug for treating EGFR high-expression tumor. It is found that enrichment of tumor stem cells caused by chemotherapeutic drugs can be weakened after the SGCE gene is inhibited; after the SGCE is inhibited, the combination of the Cbl and the EGFR is enhanced; egfr degradation, finally, the sensitivity of the EGFR inhibitor-resistant cell strain to the EGFR inhibitor is remarkably enhanced, and it is prompted that the drug resistance of tumor cells to the EGFR inhibitor can be overcome when the inhibitor for synthesizing the SGCE and the EGFR inhibitor are jointly applied to drugs for treating EGFR high-expression tumors. Therefore, proliferation and metastatic growth of various EGFR high-expression tumors such as triple-negative breast cancer, non-small cell lung cancer, colorectal cancer and the like can be effectively inhibited by jointly inhibiting EGFR while inhibiting SGCE, and recurrence of cancer is effectively prevented. The reagent has important guiding significance in clinical application and has a wide prospect.

The invention discloses a heparin starch microsphere vascular embolizing agent with anti-tumor effect and a preparation method thereof, which belong to the field of pharmaceutical and chemical industry, and concretely relates to the heparin starch microsphere vascular embolizing agent and the preparation method thereof. The technical problem to be solved by the present invention is to provide a heparin starch microsphere vascular embolization agent and the preparation method thereof. The method prepares heparin starch microspheres with controlled size and uniform particle size by introducing heparin with good biocompatibility and capable of inhibiting tumor growth and metastasis. The heparin starch microspheres not only have an embolization effect, but also inhibits tumor growth and metastasis, and achieves the purpose of treating cancer. The method has the advantages of simple operation, good repeatability and low temperature requirement, can prepare the heparin starch microspheres with good biocompatibility, degradability and no toxic side effects by controlling reaction conditions, can be widely used in tumor vascular embolization and treatment, and establishes a foundation forits application in the medical field.

The invention belongs to the field of biological medicine research, and particularly relates to application of a human SHCBP1 gene used as a target in preparing a thyroid cancer treatment medicine orpreparing a thyroid cancer diagnosis medicine. Through the broad and deep research, the invention discovers that after the expression of the human SHCBP1 gene is down-regulated by adopting an RNAi method, the multiplication of thyroid cancer cells can be effectively inhibited, the cell apoptosis is promoted, and the growth process of thyroid cancer can be effectively controlled. The siRNA or a nucleic acid building body and a slow virus containing the siRNA can specifically inhibit a multiplication capacity of the thyroid cancer cells, inhibit a tumor formation capacity of the thyroid cancer cells in a human body, promote the thyroid cancer cell apoptosis, inhibit the cloning of the thyroid cancer cells, inhibit a thyroid cancer cell metastasis capacity, inhibit a thyroid cancer cell transfer capacity and change the period distribution of the thyroid cancer cells so as to treat the thyroid cancer, so that a new direction is opened for thyroid cancer treatment.

The invention provides a derivative of a vascular endothelial growth factor receptor antagonistic peptide F56 and application thereof. The derivative of the vascular endothelial growth factor receptorantagonistic peptide F56 provided by the invention is a peptide derivative constructed by connecting a MIPA-AEEA-group on the basis of the structure of the vascular endothelial growth factor receptorantagonistic peptide F56. In addition to maintaining the antagonistic effect of the F56 on the binding of a vascular endothelial growth factor (VEGF) to a receptor 1 (VEGFR1) thereof and having anti-angiogenesis and anti-tumor activities, the peptide derivative has remarkably longer action time than a polypeptide F56, and can be used in anti-angiogenesis and anti-tumor therapy.

The invention provides application of 4-methyl-5,6-dihydropyran-2ketone in preparing anti-hepatic fibrosis and anti-hepatoma drugs, and belongs to the technical field of anti-hepatic fibrosis and anti-hepatoma drugs. It is found that the 4-methyl-5,6-dihydropyran-2ketone has good anti-hepatic fibrosis activity and activity for inhibiting the hepatoma carcinoma cell migration, and can be used for preparing the anti-hepatic fibrosis and anti-hepatoma drugs. Embodiment results show that the 4-methyl-5,6-dihydropyran-2ketone can inhibit the activity of type I collagen alpha 1 promoter, reduce theexpression of hepatic fibrosis markers such as COL1A1 and ACTA3 in hepatic stellate cells LX-2, improve the liver tissue pathological structure and hepatic fibrosis degree in BDL mice, and inhibit themigration of hepatoma carcinoma cells HepG2.

The invention relates to an application of flunarizine hydrochloride in preparation of medicines for inhibiting tumor cell metastases and spread. The metastases ability of tumor cells are inhibited by medicines prepared from flunarizine hydrochloride; flunarizine hydrochloride is a low-toxicity medicine, and can play a relatively great blocking role in a tumor spreading process; meanwhile, other related tumor medicines are used in treatment, so that the mortality of normal cells can be greatly reduced; the existing tumor medicines mainly are cytotoxic medicines; and a plurality of normal cells are killed when the tumor cells are killed.

The invention discloses verification of an ACAP4 activity inhibiting protein and functions of the ACAP4 activity inhibiting protein in cell migration. The invention aims to provide an ACAP4 activity inhibiting protein and a coding gene thereof, and application of the ACAP4 activity inhibiting protein in preparing drugs for inhibiting tumor metastasis. The protein is one of the following amino acid sequences: 1) SEQ ID NO:1 in the sequence table; and 2) protein with function of regulating ACAP4 and ARF6 activity subjected to substitution, deletion or addition of one to ten amino acid residues on the amino acid residue sequence disclosed as SEQ ID NO:1 in the sequence table. The protein is an ACAP4-activity-inhibiting protein, and can display the signal passage function and regulate the migration function of tumor cells by regulating the combining capacity with ACAP4 and the activity of the substrate ARF6. Therefore, the protein can be used as a drug target to perform screening on small-molecule compounds, thereby detecting possible drugs for inhibiting tumor cell metastasis. The protein and coding gene thereof can perform important functions in the fields of medicine and pharmacy, and have wide prospects.

The invention discloses applications of a ZNF367 gene in preparing medicines for treating breast cancer and reagents for realizing diagnosis and prognosis evaluation. The in vitro and vivo experimental result of the inventor proves that ZNF367 is highly expressed in the breast cancer tissue, and the survival analysis shows that the expression level of the ZNF367 gene is closely related to the staging and prognosis of breast cancer. Therefore, an inhibitor of the gene can be synthesized or prepared to be taken as the potential targeted medicine for breast cancer; the gene has DNA amplification and expression increase in the breast cancer, therefore, a kit for detecting the DNA variation and expression change of the gene can be prepared for diagnosing the breast cancer; the increase of the gene expression is related to the poor prognosis, and therefore, the kit for detecting the expression level change of the gene also can be used for prognosis evaluation thereof, so that the clinical treatment can be guided.

The invention discloses application of siRNA of long non-coding RNA LINC00152 and an inhibiting preparation. The siRNA sequence of the target LINC00152 is designed and synthesized, the siRNA is transfected into an oral cancer cell line, and thus it is proven that the expression of the target interfering LINC00152 can obviously inhibit the invasion and transfer ability of oral cancer cells. A new potential approach is provided for treatment of oral cancers.

The invention relates to inhibition of invasion and transferring of prostatic cancer by utilization of a targeting polar molecule Par3. A prostate cancer cell subcloning cell line of the stable knock-down Par3 is constructed, a corresponding nude mouse prostate in-situ transplanting tumor model is established, and the effects of the stable knock-down Par3 on inhibition of invasion and transferring of the prostatic cancer are verified in vitro and in vivo. After the stable knock-down Par3 is discovered, through damage of formation of a Par3-aPKC-KIBRA ternary complex, phosphorylation of an important member MST1/2 and Lats1 in a Hippo passage is enhanced, therefore the Hippo passage is activated, a protooncogene YAP at the downstream is phosphorylated, thus the cytoplasm ofthe YAP protein is retarded, nuclear import of the non-phosphorylated YAP protein is reduced, and finally interaction of YAP and TEAD transcription factors in cell nuclei is weakened, and transcription of a series of invasion/ transferring promoting genes is inhibited. The fact that the stable knock-down Par3 has functions of inhibiting invasion and transferring of prostatic cancer cells is verified in vitro, in vivo, on phenotypes and in molecular mechanism.