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Application of rabeprazole sodium in preparation of medicines for inhibiting tumor cell metastasis and spread

A technology of rabeprazole sodium and tumor cells, applied in the pharmaceutical field of rabeprazole sodium, can solve problems such as the pharmacological effect of rabeprazole sodium has not been found, and achieve the effect of reducing the number of deaths and shortening the research and development cycle.

Inactive Publication Date: 2015-04-29
南京格耀生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Other pharmacological effects of rabeprazole sodium have not been found in the existing public literature

Method used

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  • Application of rabeprazole sodium in preparation of medicines for inhibiting tumor cell metastasis and spread
  • Application of rabeprazole sodium in preparation of medicines for inhibiting tumor cell metastasis and spread
  • Application of rabeprazole sodium in preparation of medicines for inhibiting tumor cell metastasis and spread

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Application of Rabeprazole Sodium in the Preparation of Drugs for Inhibiting Metastasis and Spread of Colon Cancer Cells. The specific experimental plan is as follows:

[0025] 1. Experimental materials

[0026] Colon cancer cells (HT29) were used in the experimental stage of the present invention, and the drug used in the positive control was nocodazole (experimental concentration: 10uM). The negative control group received no treatment.

[0027] Wherein experiment adopts rabeprazole sodium compound C 18 h 20 N 3 NaO 3 S, CAS NO. is 117976-90-6, the concentrations are 100uM, 80uM, 60uM, 50uM, 40uM.

[0028] 2. Experimental method

[0029] For colon cancer cells cultured for 24 hours (cell density: 10 5 ) for drug stimulation for 2 hours:

[0030] (1) Centrifuge the colon cancer cells in a better state in the culture dish, and add an appropriate amount of culture medium to make a cell suspension;

[0031] (2) Get the cell suspension count, according...

Embodiment 2

[0039] Example 2: Transwell cell migration assay was used to determine the effect of rabeprazole sodium on the migration ability of colon cancer cells.

[0040] Will 1×10 5 The HT29 cells were inoculated in Transwell chambers with a 12.0 μm membrane at the bottom, and continued to be cultured. After the cells adhere to the wall, add DMEM complete medium with different concentrations of rabeprazole sodium to the upper chamber. To maintain osmotic pressure, add 0.05%-0.2% BSA, and add DMEM complete medium containing 15% FBS to the lower chamber and continue to cultivate 24h. The unmigrated cells on the upper surface of the membrane were carefully wiped off with a cotton swab, and the cells that migrated to the lower surface of the membrane were fixed with 2% PFA at 37°C for 10 minutes and then stained with Giemsa. Ten visual fields were randomly selected under a high-power microscope to count the migrated cells.

[0041] Cell migration rate = [the average number of cells in t...

Embodiment 3

[0045] Example 3: The damage repair experiment detects the effect of rabeprazole sodium on the migration ability of tumor cells.

[0046] Take HT29 cells in the logarithmic growth phase, and add 1×10 cells to each well of a 24-well plate 5 After the cells grow into a single layer, draw a "one"-shaped scratch, wash with PBS, add different concentrations of rabeprazole sodium in the control group and the experimental group, and take pictures. After 22 hours of culture, the same part is taken again to measure the migration distance. The experiment was repeated 3 times, and the cell migration rate=[(0h average value of the experimental group-22h average value of the experimental group) / (0h average value of the blank control group-22h average value of the blank control group)]×100%.

[0047]Rabeprazole sodium acts on LTEP and A549 cells for 22 hours at different concentrations (40-100μg / ml) to inhibit migration. The results show that rabeprazole sodium has a dose-dependent inhibito...

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Abstract

The invention discloses an application of rabeprazole sodium in preparation of medicines for inhibiting tumor cell metastasis and spread. The metastasis capability of tumor cells is inhibited by utilizing the medicines prepared from the rabeprazole sodium, the rabeprazole sodium refers to a low-toxicity medicine, a great blocking effect can be achieved in the tumor spreading process, meanwhile, treatment is performed by adopting other related tumor drugs, and the death amount of normal cells can be greatly reduced; and moreover, most of the existing tumor drugs are cytotoxic drugs, and lots of normal cells are killed when the tumor cells are killed.

Description

technical field [0001] The invention relates to a new application of rabeprazole sodium in the field of pharmacy, in particular to the application of rabeprazole sodium in the preparation of drugs for inhibiting tumor cell metastasis and diffusion. Background technique [0002] Rabeprazole sodium is a new type of proton pump inhibitor, which can be used in the treatment of acid-related diseases, such as gastric and duodenal ulcers, esophageal reflux disease, etc.; it has a selective and strong inhibitory effect on Helicobacter pylori (HP) . h 2 Receptor antagonists and proton pump inhibitors are the two most commonly used drugs for the treatment of acid-related digestive diseases. They both increase gastric pH, but proton pump inhibitors act on H+ / K+-ATPase, strongly inhibit gastric acid secretion, and Rabeprazole sodium is the latest proton pump inhibitor, and its anti-secretion activity of gastric acid is greater than that of the original proton pump inhibitor omeprazole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4439A61P35/04
CPCA61K31/4439
Inventor 董梅
Owner 南京格耀生物科技有限公司
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