54results about How to "Accelerate self-renewal" patented technology

Compositions and methods that employ various combinations of such factors as retinoic acid signaling inhibitors, antioxidants, BMP4, VEGF, prostaglandin E₂ pathway stimulants, TPO, SCF, FLT-3, EPO, TGFβ1, p38 MAPK inhibitors, beta adrenergic receptor agonists, cell cycle inhibitors, RXR agonists, Cripto, and chromatin remodelers to drive differentiation of pluripotent stem cells towards primitive blood cells. Uses of such primitive blood cells are provided.

The invention relates to a protein skin care product with skin repairing and maintenance functions. The protein skin care product contains a keratin composition, wherein the keratin composition contain the following components in mass fraction in the protein skin care product: 0.3-5% of keratin, 0.1-1% of sodium alginate and 0.1-5% of polysaccharide; the keratin has the molecular weight of 10000-70000 Dalton; and the polysaccharide is at least one of fructose, fructo-oligosaccharide, cationic fructo-oligosaccharide, hyaluronic acid and salt thereof as well as chitosan. The protein skin care product provided by the invention can promote absorption of cells on protein, so that self renewing and repairing capability of skin cells is further improved, and especially the effect of repairing skin after ultraviolet burn is improved.

The invention discloses a medicine for inhibiting proliferation of esophageal cancer cells. The roles of SOX9 in the occurrence and development of esophageal squamous cell carcinoma and a downstream molecular mechanism regulated by the SOX9 are researched from the view of tumor stem cells by detecting clinical specimens and establishing an in vitro cell model and an in vivo mouse model; experiments proves that the SOX9 is highly expressed in the tissues of the esophageal squamous cell carcinoma and in esophageal cancer stem cell balls, and can promote the proliferation and self-renewal of a cell line of the esophageal squamous cell carcinoma, the formation of tumor stem cell balls, the growth of transplantation tumor in nude mice, and the tolerance to chemotherapeutic drug cisplatin. The invention provides a theoretical basis and an experimental basis for further clarifying the occurrence and development mechanism and targeted treatment of esophageal cancer.

The invention provides a method for inducing self-renewal of mammary stem cells. The invention discloses a new application of microRNA31. Experiments show that through over-expression of microRNA31, the proportion of mammary stem cells is obviously regulated up, clone formation ability is enhanced and therefore the self-renewal of the mammary stem cells is promoted.

The invention provides application of a SphK2 inhibiting agent ABC294640 in improving hematopoietic stem cell functions and treating hematopoietic dysfunction. The ABC294640 can be used for treating essential hematopoietic dysfunction, or radiation and chemical drug irritated hematopoietic dysfunction, and is capable of improving hematopoietic stem cell functions to improve the recovery rate and the survive rate of hematopoietic function of a patient after chemotherapy is carried out for a plurality of times. According to the drug, a new drug source is provided to treat essential or induced hematopoietic dysfunction diseases.

The invention discloses an application method of small molecules capable of promoting self-status-renewal of embryonic stem cells. The application method of the small molecules capable of promoting self-status-renewal of the embryonic stem cells comprises the following steps: passaging and culturing human and mouse embryonic stem cells under small molecular state of the method; and then, observingand detecting self-renewal state of the cells according to the method. Being added with CID755673 small molecules, the application method of the small molecules capable of promoting self-status-renewal of embryonic stem cells can solve the problems of liable state differentiation, complex passages operation, high culture-condition cost, unfavorable follow-up mechanism research and so on of humanand mouse embryonic stem cells under existing traditional cultivation conditions; moreover, the application method of the small molecules capable of promoting self-status-renewal of embryonic stem cells has the advantages of being stable in effects, economical, highly efficient, easy to operate, convenient to study and so on. In addition, clues are further provided for improving and establishing culture conditions of pluripotent stem cells of other species and genera; and thus, the application method of the small molecules capable of promoting self-status-renewal of embryonic stem cells playsan active role in promoting smooth development of basic and applied research of stem cells in the future.

The invention belongs to the technical field of biological medicines, and provides a preparation method of hESC-MSC. The method comprises that a supporting/coating material is changed and a culture solution system is changed. Embryonic stem cells (hESCs) start a differentiation process by a method based on mechanical partitioning and planking. The hESC gradually enters the differentiation mode, and the high survival rate is kept in the differentiation process, so that the better differentiation efficiency is achieved. Meanwhile, screening is performed by adjusting a passage mode, adopting a partitioning method and then adopting a digestion method according to the growth characteristics of hESC and MSC, so that the operation is simple and convenient, and the effect is obvious. The obtainedhESC-MSC can be used for inhibiting CD4+T-cell proliferation and promoting the increasing of CD4+Foxp3+(Treg). The hESC-MSC has an immunomodulatory capacity equivalent to or higher than that of umbilical cord MSCs and has powerful proliferative capacity and a simple, convenient and efficient differentiation effect in a short time, and derived cells have typical MSC cytological characteristics.

The invention, which belongs to the related technical field of skin care products, discloses skin care serum containing stem cell active factors and application thereof. The skin care serum comprisesthe following components, by weight: 10 to 20 parts of a stem cell active factor extracting solution, 0.08 to 0.2 part of silk protein, 0.2 to 0.6 part of asiaticoside, 1 to 2 parts of yeast extract,0.2 to 2 parts of allantoin, 0.05 to 2 parts of tocopherol, 5 to 8 parts of squalane, 0.3 to 1 part of olive oil, .5 to 0.8 part of EDTA-2Na, 0.05 to 0.2 part of triethanolamine, 0.6 to 3 parts of polyglutamic acid, 0.05 to 0.2 part of vitamin C, 5 to 10 parts of glycerol and 80 to 100 parts of water. According to the invention, a large number of bioactive factors beneficial to skin regeneration and repair, especially stem cell active factors, are contained in the formula, so that skin self-renewal and regeneration are promoted and the striae gravidarum repair effect is enhanced.

The invention provides a haemorrhoid paste which has the functions of analgesia, antiphlogosis and detumescence, takes effect fast, and uses Zingiber corallinum Hance essential oil as the main curative component. The haemorrhoid paste is prepared from the following raw materials in parts by weight: 0.5-3 parts of Zingiber corallinum Hance essential oil, 0.5-2 parts of zanthoxylum oil, 2-5 parts of glycerin, 70-82 parts of vaseline and 15-20 parts of beeswax. The preparation method comprises the following steps: heating beeswax and vaseline commonly to melt and then boil, cooling to 35-45 DEG C, adding Zingiber corallinum Hance essential oil, zanthoxylum oil and glycerin to stir evenly, cooling, and sealing for 7-10 days to obtain the finished product. The haemorrhoid paste has obvious effects of analgesia, antiphlogosis and detumescence and can improve microcirculatory disturbance, promote blood circulation and accelerate the recovery of patients with haemorrhoid. The haemorrhoid paste takes effect fast, and has short treatment period, high recovery rate and no toxic or side effects.

The invention belongs to the technical field of cosmetics, and discloses a compound plant antioxidant mixture as well as a preparation method and application thereof. The composition comprises the following components: a rosa roxburghii tratt fruit extract, an acanthopanax senticosus extract, a caesalpinia spinosa fruit extract, a capparis spinosa fruit extract and a kiwi fruit extract. According to the composition, the mode that five plant extracts including the rosa roxburghii tratt fruit extract, the acanthopanax senticosus extract, the caesalpinia spinosa fruit extract, the capparis spinosa fruit extract and the kiwi fruit extract are combined with one another is adopted, so that better free radical scavenging performance is achieved, and the kiwi fruit extract has an obvious synergistic effect on scavenging free radicals; and the composition has a synergistic anti-wrinkle effect on skin wrinkles, so that the skin is rich in elasticity and luster.

The invention discloses a formula for preparing a mask by a Xinhui orange flower extracting solution. The formula comprises the following components in percentage by mass: 1.0 to 8.0% of Xinhui organflower extracting solution, 0.5 to 2.0% of pearl powder, 5.0 to 10.0% of anti-oxidizing compound, and 80.0 to 93.5% of cosmetic auxiliary material substrate. The invention also discloses a technologymethod of the mask using the Xinhui orange flower extracting solution. The formula has the advantages that the Xinhui orange flower extracting solution is compounded with other anti-oxidizing matters,so that the function is mild, the irrigation is avoided, and the self-updating and repair of skin cells are accelerated; the obvious anti-aging, whitening and moisturizing effects are realized, the skin quality can be quickly improved, the skin elasticity is improved, and the wrinkles are smoothed.

The use of a medical therapeutic ozone agent in vivo to treat a variety of disorders including, but not limited to hypoxic conditions, autoimmune disease, fibrotic disease, inflammatory disease, neurodegenerative disease, infectious disease, lung disease, heart and vascular disease, metabolic disease, and cancer, wherein the therapeutic ozone agent is administered in a therapeutically effective amount, which can convert carbon monoxide to carbon dioxide, thereby treating or preventing the disease in a patient.

The use of a medical therapeutic ozone agent in vivo to treat a variety of disorders including, but not limited to hypoxic conditions, autoimmune disease, fibrotic disease, inflammatory disease, neurodegenerative disease, infectious disease, lung disease, heart and vascular disease, metabolic disease, and cancer, wherein the therapeutic ozone agent is administered in a therapeutically effective amount, which can convert carbon monoxide to carbon dioxide and significantly raise systemic oxygen partial pressure to increase the delivery of oxygen to all tissue, systemically, to enable the reversal of hypoxic conditions of the human and animal body and the consequences of those hypoxic conditions, thereby treating or preventing the disease in a patient.

The invention discloses a credible knowledge ecosystem which comprises an automatic learning resource collection module for collecting learning materials with specific themes from internet open resources according to needs, carrying out data cleaning and data reduction, and storing structured or semi-structured data in an internal learning resource library credibly; The resource learning module learns resources to form a personal knowledge base, and the personal knowledge base is stored on the platform in a credible mode; The knowledge management module organizes and fuses knowledge elements in the personal knowledge base and updates the knowledge elements to an existing knowledge system; The knowledge sharing module is used for credibly sharing knowledge to a specific user (group); The knowledge innovation module is combined with an existing knowledge system to produce personal achievements and form a personal achievement library; And the achievement submitting module is used for submitting the personal achievements to a platform. In the invention, the knowledge flowing process promotes the continuous self-updating of the knowledge base to form a knowledge ecosystem with continuous circulation and self-growth.

The invention discloses a formula of shower gel made from Xinhui orange peel and orange flower extract and a process. The formula comprises the following components in percentage by mass: 5-10% of sodium laureth sulfate, 1-5% of ammonium lauryl sulfate, 0.2-0.5% of polyquaternium-7, 0.1-0.3% of PEG-120 methyl glucose dioleate, 0.1% of methylparaben, 0.1-0.4% of polyoxyethylene sorbitan tristearate, 2-7% of cocamidopropyl betaine, 1-6% of cocoanut fatty acid diethanolamide, 0.5-1% of Xinhui orange peel extract, 0.5-1% of Xinhui orange flower extract, 1-5% of glycerin, 0.1-0.5% of chitosan, 0.1-0.2% of EDTA (Ethylene Diamine Tetraacetic Acid) disodium and the balance of water. The Xinhui orange peel and orange flower extract is combined with other moisturizing components, so that the made shower gel is mild in effect and non-irritating, and can achieve the effects of accelerating self-renewal and repair of skin cells and achieving skin repair and moisturize and moistening effects while cleansing skin.

The invention belongs to the technical field of biology, and in particular relates to a method for promoting the over-expression of TSP4 in mesenchymal stem cells through lentivirus infection. The pENTR11-TSP4 expression plasmid is obtained through the pCMV6-Thbs4 plasmid and a pENTR11 plasmid vector; then the pENTR11-TSP4 expression plasmid and Plv-Easy-T plasmid are connected to obtain Plv-Easy-GFP-TSP4 lentiviral expression plasmid; then Plv-Easy-GFP-TSP4 lentivirus is obtained through packaging; and finally the Plv-Easy-GFP-TSP4 lentivirus is used to infect mesenchymal stem cells. Viral gene fragments are integrated into the genome of the mesenchymal stem cells to obtain TSP4-over-expressed mesenchymal stem cells, so that the activation efficiency of the TSP4-over-expressed mesenchymalstem cells for promoting angiogenesis is improved, and thereby the TSP4-over-expressed mesenchymal stem cells are applied in drugs for the treatment of ischemic diseases, and especially applied in drugs for the treatment of ischemic stroke.