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Cosmetic uses of dedifferentiated plant cells

A plant cell, dedifferentiation technology, applied in the field of beauty, can solve problems such as functional changes, and achieve the effect of improving expression

Active Publication Date: 2019-06-14
LOREAL SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Available data suggest that the number of regenerative cells in the epidermis does not decrease with age (Giangreco et al., Aging Cell, 2008, 7:250), but their functionality appears to be greatly controlled by microscopic changes in the regenerative capacity of the basal layer. The impact of a change in the environment

Method used

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  • Cosmetic uses of dedifferentiated plant cells
  • Cosmetic uses of dedifferentiated plant cells
  • Cosmetic uses of dedifferentiated plant cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0195] Dedifferentiation of Plant Cells Using Plant Tissue Culture Rosebush (Rosa lancomee)

[0196] The blades of rosebush (Lancome rose) are purified by conventional technical means of those skilled in the art, and then placed on the following medium:

[0197] macronutrients

mg / l

NH 4 NO 3

1650

KNO 3

1900

CaCl 2 2H 2 O(CX41)

440

MgSO 4

180.8

K H 2 PO 4

170

micronutrients

mg / l

MnSO 4 4H 2 o

22.3

ZnSO 4 ·7H 2 o

8.6

KI

0.83

Na 2 MoO 4 2H 2 o

0.25

CuSO 4 ·5H 2 o

0.025

Na 2 EDTA·2H 2 o

37.3

FeSO 4 ·7H 2 o

27.8

vitamins

mg / l

Inositol

100

Niacin

0.5

Pyridoxine hydrochloride (B 6 )

0.5

Thiamine hydrochloride (B 1 )

0.1

hormone

mg / l

Naphthaleneacetic acid

10

kinetin

0.06

carbon source

mg / l

sucrose

...

Embodiment 2

[0205] Demonstrating the "pro-regenerative" effect of dedifferentiated plant cells of Lancome rosebush in a model of reconstructed skin with low regenerative potential

[0206] a - Preparation of a model of reconstructed skin with low regenerative potential

[0207] Isolation of keratinocytes from human skin samples.

[0208] After removing the subcutaneous tissue with a scalpel, the skin samples were cut into pieces and then decontaminated by antibacterial-antifungal treatment in DMEM medium (Life Technologies, Scotland).

[0209] In order to separate the dermis from the epidermis, the samples were then subjected to proteolytic treatment (Dispase II) / Trypsin 0.25%) overnight at 4°C. Epidermal fragments, separated from dermal tissue, were placed in 1x trypsin-EDTA solution at 37°C for 20 minutes. The effect of trypsin was neutralized by adding medium containing 10% fetal calf serum (FCS). The cell suspension was homogenized and then washed with keratinocyte growth medium ...

Embodiment 3

[0223] Titration of Keratinocyte Growth Factor (KGF) Secretion Induced by Treatment of Rosebush Dedifferentiated Plant Cells in a Model of Reconstructed Skin with Low Regenerative Potential

[0224] Culture media for this experiment described in Example 2 were harvested on days 3, 5, 7, 11, 13, 17 and 20. The soluble amount of KFG secreted by the reconstituted skin and present in these media was analyzed by ELISA (Enzyme-Linked Immunosorbent Assay) (R&D Systems, catalog N° DKG00).

[0225] Such as figure 2 As shown, an increase in KGF secretion was observed, especially at day 13 after treatment with rose dedifferentiated cells (0.43 mg / ml) compared to the control (untreated).

[0226] The growth factor is produced by fibroblasts and is well known for its mitogenic effect on keratinocytes (Finch et al., Science 1989, 245:752; Marchese et al. / J Cell Physiol 1990, 144:326). This result shows that rose (Rosa lancomee) dedifferentiated plant cells induce the secretion of diffusi...

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Abstract

The present invention relates to the cosmetic use of dedifferentiated plant cells of Rosa plants or of extracts or lyophilizates of said cells as active agents for the care of aging skin or aging hair. The present invention also relates to rose dedifferentiated cells, especially rose dedifferentiated cells of Lancome.

Description

technical field [0001] The invention belongs to the field of cosmetics, and relates to the use of dedifferentiated plant cells of plants of the genus Rosa (Rosa sp.) as an active agent for aesthetic care of skin and hair. [0002] The present invention also relates to extracts derived from Lancome rosebush ( rosebush) of dedifferentiated plant cells capable of and effectively restoring the regenerative capacity of the skin. [0003] In particular, the present invention relates to a cell line derived from dedifferentiated plant cells of Rosa rosebush of the Lancome variety, capable and effective in restoring the regenerative capacity of a model of reconstructed skin with a low regenerative potential, and to the source It is effective in the self-renewal ability of dermal stem cells and skin stem cell (SKP) reservoir (reservoir) of human skin biopsy (scalp and foreskin). Background technique [0004] Skin is a tissue that continuously renews itself throughout the life of an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61Q5/00A61Q19/08A01H5/02A61K8/9783
CPCA61Q5/00A61Q19/08A01H6/749A61K8/9789A61K8/9783A61Q5/002A61Q19/00C12N5/04
Inventor 理查德·马丁玛丽莱恩·帕里斯米歇尔·拉思曼乔斯兰德布里吉特·拉沃德帕斯卡·希莱尔
Owner LOREAL SA
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