The invention relates to a pigeon nest sterilization spray for boiler pigeon breeding and a preparation method thereof, and relates to the technical field of boiler pigeon breeding. The pigeon nest sterilization spray for boiler pigeon breeding is prepared from the following raw materials: leaf of magnolia officinalis, zingiber oil, leaf of Chinese ilex, folium perillae, sweet potato vine, coal ash, folium forsythia, hydrogenated palm oil, medical stone, rice wine lees, ardealite powder, ethyl alcohol, sesbania gum, an emulsifier, Chinese honeylocust fruit, compound calamine lotion and water. The preparation method comprises the following steps of: mashing plant traditional Chinese medicines, adding the mashed traditional Chinese medicines into the water to soak, heating to boil down, adding the rice wine lees and the ethyl alcohol, and filtering to obtain filtrate a; grinding the coal ash, the medical stone and the ardealite powder into powder, and after sufficiently mixing the powder with the compound calamine lotion, obtaining a material b; sufficiently mixing the zingiber oil and the hydrogenated palm oil to obtain a material c; heating the filtrate a, then adding the material b and the material c into the filtrate a, sufficiently stirring, and adding the sesbania gum and the emulsifier. The pigeon nest sterilization spray for boiler pigeon breeding, which is disclosed by the invention, is good in sterilization effect, high in response speed, high in efficacy, good in adhesion, long in efficacy acting time and good in prevention and treatment effect on various boiler pigeon diseases.