A mask with excellent moisturizing and repairing effects

A facial mask and membrane-penetrating peptide technology, which is applied to medical preparations containing active ingredients, skin care preparations, and cosmetic preparations, etc., can solve problems such as high cost, difficult mass production of EGF, and difficulty in fully exerting its efficacy. To achieve the effect of enhancing solubility

Active Publication Date: 2022-07-29
四川省恩乐生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Epidermal Growth Factor (EGF) is an important cell growth factor secreted by the human body and has strong physiological activity. However, EGF has not been widely used in the fields of medicine and cosmetics. The reason is that EGF is difficult to achieve in batches. Chemical production: At present, the extraction methods of EGF are mainly divided into two types, one is from animal sources, such as mouse jaw, bovine brain, etc., and the other is from the culture supernatant of stem cells. The two methods are obtained The content of EGF is very small, and the cost is extremely high
In addition, the molecular weight of the usual EGF is about 6000 Daltons, the molecular weight is relatively large, and the penetrating power to the skin is poor, and its efficacy is difficult to fully exert

Method used

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  • A mask with excellent moisturizing and repairing effects
  • A mask with excellent moisturizing and repairing effects
  • A mask with excellent moisturizing and repairing effects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Acquisition of target DNA

[0034] (1) The gene sequence of the target gene is:

[0035] Using 5'-GCAAGCGCATTGCACATGTACGGCTATAGCAGATA-3' (SEQ ID NO: 1) with the target gene transcribed messenger RNA as a template, reverse transcribed into complementary single-stranded DNA, and then synthesize double-stranded DNA under the action of T4DNA ligase, that is, the purpose Gene.

[0036] (2) PCR amplification

[0037] Denaturation: high temperature dissociates double-stranded DNA into single-stranded DNA (94°C, 30s);

[0038] Annealing: the primer (pACT2 GAL4 AD pACT2-R) binds to the complementary region of the template DNA at low temperature to form a hybrid molecule (55°C, 30s);

[0039] Extension: mesophilic extension, in DNA polymerase, dNTP, Mg 2+ In the presence of DNA polymerase, DNA polymerase catalyzes the extension of the DNA strand starting from the primer from 5' to 3' to synthesize a DNA daughter strand complementary to the template DNA strand (70-7...

Embodiment 2

[0041] Example 2: Electrophoresis detection and recovery and purification

[0042] (1) Electrophoresis

[0043] ① Prepare an appropriate amount of buffer for electrophoresis and gel preparation (1X TAE Buffer)

[0044]②Accurately weigh the agarose powder (1g) according to the amount of gel and gel concentration, and add it to an appropriate conical flask.

[0045] ③ Add a certain amount of electrophoresis buffer (1X TAE Buffer 100ml).

[0046] ④ After melting is complete, cool to about 60℃, add EB5-7ul, and mix well.

[0047] ⑤Pour the solution into the plastic mold, before inserting a comb in the appropriate position.

[0048] ⑥It will solidify at room temperature. If it is not used immediately, wrap the gel with plastic wrap and store it at 4℃.

[0049] Mix an appropriate amount of PCR product with an appropriate amount of bromophenol blue, add it to the gel tank, and add an appropriate amount of DNAMaker to the right tank to start electrophoresis. Observing the electro...

Embodiment 3

[0055] Example 3: Construction of recombinant expression vector

[0056] The selected recombinant expression vector plasmid vector is pBR322, and the construction of the recombinant plasmid includes the following steps:

[0057] (1) Since one of the parents of the pBR322 plasmid is the pMB1 plasmid, firstly, based on pMB1, the Tn3 translocation of the Rldrd19 plasmid was introduced to obtain a 13.3kb pMB3 plasmid;

[0058] (2) The molecular weight of pMB3 obviously makes it unsuitable as a carrier, so most of the useless fragments must be lost by digestion under the conditions of EcoRI activity, and the sticky ends of the remaining small fragments of DNA are ligated and formed. pMB8 plasmid (2.6kb);

[0059] (3) At this time, another pSC101 plasmid was digested under EcoRI activity conditions to produce a tetr-resistant DNA fragment, which was integrated with pMB8 to form pMB9 plasmid (5.3kb). At this time, pMB9 is ampstetr phenotype;

[0060] (4) pMB9 has preliminarily fun...

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Abstract

The present invention provides a facial mask with excellent moisturizing and repairing effects, the components of which include a polypeptide and a moisturizing agent. Mixed active protein peptides obtained by mixing the small molecule penetrating peptides shown in 3. The mask formula of the present invention is compounded with TAT-EL mixed active protein peptide: it replaces tissue / collagen / capillary to receive free radical attack, so as to protect capillaries and collagen; it has excellent functions of promoting cell proliferation and differentiation, accelerating The self-renewal and self-repair of skin epidermal cells has a good effect on the repair of damaged acne muscles / medical and cosmetic wounds. The acetylated hyaluronic acid used in the present invention, as one of the moisturizing agents, enhances its solubility, permeability and resistance to hyaluronidase; It is also soluble in oil, and can use the intercellular substance as a carrier to penetrate into the basal layer of the skin and exert a moisturizing effect.

Description

technical field [0001] The invention relates to a facial mask and a preparation method thereof, in particular to a facial mask with excellent repairing function and a preparation method thereof. Background technique [0002] Polypeptides are usually added to high-end masks, and the peptides in the mask have the functions of cell renewal, metabolism, growth, and repair. Epidermal Growth Factor (EGF) for short is an important cell growth factor endocrine in the human body. It has strong physiological activity. However, EGF has not been widely used in the field of medicine and cosmetics. The reason why EGF is difficult to achieve in batches Chemical production: At present, EGF extraction methods are mainly divided into two types, one is from animal sources, such as mouse mandibles, bovine brains, etc., and the other is extracted from the culture supernatant of stem cells. The content of EGF is very small and the cost is extremely high. In addition, the molecular weight of ord...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/64A61K8/73A61K8/9789A61K8/9794A61Q17/00A61Q19/00A61Q19/08
CPCA61K8/64A61K8/735A61K8/9794A61K8/9789A61Q19/00A61Q19/08A61Q17/005A61K2800/5922
Inventor 宋筱荭邹弼丞杨丹
Owner 四川省恩乐生物工程有限公司
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