Polyphenol extract of trembling poplar as well as extraction method and application thereof

A technology of tulip tree and polyphenols, which is applied to the polyphenol extract of tulip tree and its extraction field, can solve problems such as unresearched medicinal value of hybrid trees, achieve good economic and social benefits, inhibit the The production and preparation process is simple

Active Publication Date: 2012-02-01
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the medicinal value of hybrid trees has not been studied, especially in the research of drugs for the treatment of tumors

Method used

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  • Polyphenol extract of trembling poplar as well as extraction method and application thereof
  • Polyphenol extract of trembling poplar as well as extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take freshly collected tulip tree bark and dry it in air at 35°C. Grind the dry material to medium fine powder, about 40 mesh, and set aside.

[0022] Take 350g of fine powder, then extract with 95% ethanol, and extract 3 times with 500ml; filter, combine the filtrates, and evaporate to remove the solvent to obtain a residue of about 35.0g; dissolve the residue with chloroform, dissolve 3 times in 250mL, distill off the chloroform solvent to obtain 8.4g of the product LTB2 and the first residue; the first residue was dissolved with methanol, 250mL was dissolved three times, and the solvent was distilled under reduced pressure to obtain 13.3g of the product LTB3. Put each product into a sterile screw cap bottle and store at -20°C for later use. For bioassays, each extract was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mg / mL and stored at -20°C.

[0023] The method of extracting dried leaves and bark of other Liriodendron species is the same as abov...

Embodiment 2

[0032] Take freshly collected tulip tree bark and dry it in air at 35°C. Grind the dry material to medium fine powder, about 40 mesh, and set aside. Get fine powder 350g, then extract with 90% ethanol, 500ml extract 3 times; Filter, combine filtrate, evaporate to remove solvent, obtain residue; Dissolve residue with chloroform, dissolve 3 times in 250mL, distill and remove chloroform solvent, obtain product LTB2 and The first residue: dissolve the first residue with methanol, dissolve 250 mL three times, and distill the solvent under reduced pressure to obtain the product LTB3. Put each product into a sterile screw cap bottle and store at -20°C for later use. The method of extracting dried leaves and bark of other Liriodendron species is the same as above.

[0033] Table 3 shows the extract yields of different plant tissues.

[0034] Table 3 Extract yield table of different plant materials

[0035]

[0036] In the table, LT: Liriodendron tulipifera; LC: Chinese mandarin...

Embodiment 3

[0041] MCF-7 cells were cultured using ATCC complete medium, including basic medium EMEM (Eagle's Minimum Essential Medium), balanced salt solution EBSS (Earles Balanced Salt Solution), non-essential amino acids, 2mM L-glutamine, 1 mM sodium pyruvate , 1.5mg / mL sodium bicarbonate solution, 0.01mg / mL bovine insulin, 10% fetal bovine serum, 25cm containing 100U / mL penicillin and 100 mg / mL streptomycin 2 Tissue culture flasks. Temperature controlled at 37°C, in the presence of 5% (v / v) CO 2 incubated in saturated water vapor.

[0042] Breast cancer MDA-MB-231 cells were cultured with ATCC complete medium, including L-15 and 2 mM L-glutamine, 1.5 mg / mL sodium bicarbonate, 10% fetal bovine serum, containing 100 U / mL penicillin and 100 mg / mL 25 cm mL of streptomycin 2 Tissue culture flasks, temperature controlled at 37°C, in 5% (v / v) CO 2 incubated in saturated water vapor.

[0043]The culture conditions of the other three human tumor cells were the same as the above two, excep...

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Abstract

The invention discloses a polyphenol extract of trembling poplar as well as an extraction method and application thereof. The extraction method of the polyphenol extract of trembling poplar comprises the following steps: baking a trembling poplar plant material at 35 DEG C, and crushing; extracting with 95% ethanol, distilling to remove a solvent to obtain residues; dissolving the residues with chloroform, filtering to obtain filtrate and a primary residue, and distilling the filtrate in reduced pressure to obtain the polyphenol extract; and dissolving the primary residue by use of methanol, and distilling the solvent at reduced pressure to obtain the polyphenol extract. The polyphenol extract of the trembling poplar is convenient to extract, has high content, can be applied to industriallarge-scale production, has remarkable effects in removing oxygen radical of animals, inhibiting generation, proliferation and transfer of tumor cells of animals, is practical in treating cancer cells, and can generate good economical and social benefits.

Description

technical field [0001] The invention relates to a tree tissue extract and its extraction method and application, in particular to a polyphenol extract of Liriodendron tulipifera and its extraction method and application. Background technique [0002] There are only two species of Liriodendron genus in the family Magnoliaceae, one is Liriodendron tulipifera, which is naturally distributed in the eastern United States, and the other is Liriodendron japonica, which is distributed in southern my country. Both species have historical records of pharmacy in the United States, China, and other countries around the world. Liriodendron tulipifolia, tulip tree or boxwood, is a tall tree, often reaching a hundred feet in height, endemic to the eastern United States (Keeler, 1902). The bark of Liriodendron tulipifera was used by the Indians as a tonic, stimulant, and antipyretic, and possibly in the treatment of intermittent fevers caused by malaria (Rafinesque et al., 1828). The hist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P35/00A61K36/57A61K127/00A61K129/00
Inventor 陈金慧施季森贾爱群魏继福
Owner NANJING FORESTRY UNIV
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