Heparin starch microsphere vascular embolizing agent with anti-tumor effect and preparation method thereof

A technology of anti-tumor effect and vascular embolism, which is applied in the field of medicine and chemical industry, and can solve the problems of single chemical properties, weak binding force between drugs and microspheres, etc.

Inactive Publication Date: 2018-12-18
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Starch microspheres have shown good prospects as liver cancer embolization agents in the treatment of liver cancer, but their chemical properties are single, and there are many shortcomings in drug loading only by physical adsorption, such as weak binding force between drugs and microspheres.

Method used

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  • Heparin starch microsphere vascular embolizing agent with anti-tumor effect and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0024] (1) Preparation of water phase: add 0.1g starch and 0.1g heparin into 2ml deionized water, dissolve evenly, add 2mol / L NaOH solution, adjust pH=12, cook in an oil bath at 100°C until light yellow, and dissolve Cool to 35°C and set aside;

[0025] (2) Preparation of oil phase: Add 0.1g Span60 to 10ml soybean oil, stir at 60°C until Span60 is completely dissolved, cool the solution to 50°C, and set aside;

[0026] (3) Preparation of heparin starch microspheres: Slowly add the prepared water phase into the oil phase, control the stirring speed at 500r / min, observe under the microscope that the size of the emulsified micelles meets the requirements, then add 1ml of epichlorohydrin, at 50°C Reacted for 6 hours; the microspheres were separated by centrifugation, washed repeatedly with cyclohexane, ethanol, and acetone for 3 times, and vacuum-dried at 60°C to obtain heparin starch microspheres.

Embodiment 2

[0028] (1) Preparation of water phase: add 0.5g starch and 0.5g heparin into 2mL deionized water, dissolve evenly, add 2mol / L NaOH solution, adjust pH=12, boil in an oil bath at 100°C until light yellow, the solution Cool to 35°C and set aside;

[0029] (2) Preparation of the oil phase: add 0.2g Span60 to 40ml soybean oil, stir at 60°C until Span60 is completely dissolved, cool the solution to 50°C, and set aside;

[0030] (3) Preparation of heparin starch microspheres: Slowly add the prepared water phase into the oil phase, control the stirring speed at 500r / min, observe under the microscope that the size of the emulsified micelles meets the requirements, then add 1ml of epichlorohydrin, at 50°C Reacted for 6 hours; the microspheres were separated by centrifugation, washed repeatedly with cyclohexane, ethanol, and acetone for 3 times, and vacuum-dried at 60°C to obtain heparin starch microspheres.

Embodiment 3

[0032] (1) Preparation of water phase: add 0.8g starch and 0.8g heparin into 4mL deionized water, dissolve evenly, add 2mol / L NaOH solution, adjust pH=12, cook in an oil bath at 100°C until light yellow, the solution Cool to 35°C and set aside;

[0033] (2) Preparation of the oil phase: add 0.2 g of Span60 to 50 ml of soybean oil, stir at 60°C until Span60 is completely dissolved, cool the solution to 50°C, and set aside;

[0034] (3) Preparation of heparin starch microspheres: Slowly add the prepared water phase into the oil phase, control the stirring speed at 500r / min, observe under the microscope that the size of the emulsified micelles meets the requirements, then add 1ml of epichlorohydrin, at 50°C Reacted for 6 hours; the microspheres were separated by centrifugation, washed repeatedly with cyclohexane, ethanol, and acetone for 3 times, and vacuum-dried at 60°C to obtain heparin starch microspheres.

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Abstract

The invention discloses a heparin starch microsphere vascular embolizing agent with anti-tumor effect and a preparation method thereof, which belong to the field of pharmaceutical and chemical industry, and concretely relates to the heparin starch microsphere vascular embolizing agent and the preparation method thereof. The technical problem to be solved by the present invention is to provide a heparin starch microsphere vascular embolization agent and the preparation method thereof. The method prepares heparin starch microspheres with controlled size and uniform particle size by introducing heparin with good biocompatibility and capable of inhibiting tumor growth and metastasis. The heparin starch microspheres not only have an embolization effect, but also inhibits tumor growth and metastasis, and achieves the purpose of treating cancer. The method has the advantages of simple operation, good repeatability and low temperature requirement, can prepare the heparin starch microspheres with good biocompatibility, degradability and no toxic side effects by controlling reaction conditions, can be widely used in tumor vascular embolization and treatment, and establishes a foundation forits application in the medical field.

Description

technical field [0001] The invention discloses a heparin starch microsphere vascular embolization agent with anti-tumor effect and a preparation method thereof, belongs to the field of medicine and chemical industry, and in particular relates to a heparin starch microsphere vascular embolization agent and a preparation method. Background technique [0002] As a new treatment method developed in recent years, Transcatheterarterial embolization (TAE) is a minimally invasive medical technology guided by medical imaging equipment. It has the characteristics of small trauma, fast recovery, and good curative effect. It is used in the treatment of tumors that cannot be surgically removed. Transcatheter arterial chemoembolization (TACE) treatment is an important technique in interventional therapy. It is to insert vascular embolism materials and antineoplastic drugs into the catheter of the target artery under ray fluoroscopy to perfuse chemotherapy drugs and embolize them with embo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/001A61L24/08A61L2300/236A61L2300/416A61L2300/622C08L3/02C08L5/10
Inventor 王毅庆顾毅旭谢曼曼王建全卢乾张旭东倪轲娜韩久园
Owner NANJING UNIV
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