Fumor invasion and metastasis resisting function and use of venin cysteine proteinase inhibitor

A cysteine ​​protease and inhibitor technology, applied in the field of medical biology, can solve the problem that snake venom cysteine ​​protease inhibitors have not been reported in literature and the like
CN1781551AInactive Publication Date: 2006-06-07FUJIAN MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUJIAN MEDICAL UNIV
Publication Date
2006-06-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses the tumor invasion and metastasis resisting function and application of venin cysteine proteinase inhibitor, and belongs to the field of biomedicine. Through designing and synthesizing sv-Cystatin cDNA according to 99 amino acid sequences of Chinese cobra venin Cystatin protein, cloning to pPICZ alphaA vector and transforming Pichia yeast, stable and high expression engineering bacterium GS115-sv-Cystatin is screened out. Through further inducing expression and purification, the extracorporeal bioactivity experiment shows that the recombinant sv-Cystatin protein has the functions of inhibiting the activity of papain and inhibiting the tumor cell invasion and metastasis obviously, and so do the intracorporeal experiment. The present invention also constitutes pcDNA-sv-Cystatin eukaryotic expression vector. The present invention has huge application foreground in preventing and treating tumor.
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Description

technical field

[0001] The invention relates to the field of medical biology, in particular to a snake venom cysteine ​​protease inhibitor for anti-tumor invasion and metastasis and its application. Background technique

[0002] According to clinical statistics, more than 80% of tumor patients died of invasion and metastasis. If the tumor does not invade and metastasize, the prognosis is generally good. For example, ovarian cysts and lipomas can grow to more than ten kilograms or more. Can fully restore health. Therefore, tumor invasion and metastasis are one of the most important factors affecting the survival of cancer patients.

[0003] The invasion and metastasis of tumor cells is a series of complex, multi-step and multi-factor interaction dynamic process between tumor cells, host cells and extracellular matrix. Many data have proved that proteolytic enzymes secreted by tumor cells, including metalloproteinases and cysteine ​​proteases, play an important role in the p...

Claims

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