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A technology for liver cancer and subjects, applied in the field of cancer to achieve precise and comprehensive positioning
Pending Publication Date: 2022-06-24
THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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For example, as a cell proliferation marker, Ki67 only marks the actively proliferating liver cancer cells, which has certain limitations in accurately determining the range of cancer focus
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Embodiment 1
[0034] Embodiment 1. A kind of diagnosis method of liver cancer in subject
[0035] The inventors of the present invention found that by immunofluorescence staining, the level of ATF4 protein was low in non-HCC tissue samples (12 samples in total), but a large amount of ATF4 protein was observed in tissue samples from patients with liver cancer (24 samples in total) Therefore, confirming the expression level of ATF4 protein in tissue samples can be used in the detection, diagnosis and prognosis of liver cancer.
[0036] The present invention provides a method for diagnosing liver cancer in a subject, comprising:
[0037] 1) Determining the level of transcriptional activator 4 in a subject sample, wherein the sample is a biological fluid sample, and
[0038] 2) Compare the level obtained in 1) with the reference value, where:
[0039] If the level of transcription activator 4 in the subject's sample is increased compared with the reference value, the subject is diagnosed with...
Embodiment 2
[0047] Example 2. Identification of human hepatocellular carcinoma using ATF4 protein expression
[0048] 1) Sampling of the patient's liver tumor and paracancerous tissue: During the operation, the patient's liver containing both tumor and paracancerous tissue samples was taken, and then quickly frozen or immersed in 4% PFA for preservation.
[0049] 2) Fixing and filming of sampling samples: For quick-frozen samples, they can be placed in OCT and stored at -80°C; for samples immersed in 4% PFA, they can be embedded in paraffin. Use a cryostat or a paraffin microtome for sectioning with a thickness of 5 μm.
[0050] 3) Sample staining treatment:
[0051] For frozen sections: after washing three times with phosphate buffered saline (PBS), block with PBS containing 0.5% Triton-X100 and 3% donkey serum for 30 minutes; incubate overnight with blocking buffer containing ATF4 antibody.
[0052] For paraffin sections: perform dewaxing and hydration in the order of xylene, absolute...
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Abstract
The invention relates to the field of cancers, in particular to a biomarker beneficial to liver cancer diagnosis and prognosis, and the change level of the biomarker (transcription activator 4) in a subject sample is beneficial to liver cancer diagnosis of a subject or prognosis of a liver cancer subject. Compared with a traditional biomarker, the ATF4 protein can more comprehensively mark liver cancer cells and has obvious expression difference in liver cancer lesions, the range of the cancer lesions can be confirmed through detection, and the tumor invasion range can be judged more easily; the traditional tumor biomarker Ki67 only marks proliferative active liver cancer cells, while ATF4 protein not only can mark Ki67 positive cells, but also shows high expression in the whole liver tumor tissue compared with para-carcinoma tissue. In addition, the ATF4 protein is combined with other liver cancer biomarkers, so that cancer focuses can be positioned more accurately and comprehensively.
Description
technical field [0001] The present invention relates to the field of cancer, and in particular, provides a biomarker that is beneficial for the diagnosis and prognosis of liver cancer, wherein the change level of the biomarker in the sample of the subject is helpful for the diagnosis of liver cancer in the subject or the prognosis of the subject with liver cancer. Prognosis. Background technique [0002] Early screening and accurate diagnosis and classification of cancer are crucial for the treatment and prognosis of cancer patients. Currently, histopathological evidence based on specific biomarkers is the "gold standard" for the diagnosis of the vast majority of cancers. By taking samples from suspected cancer foci, lymph nodes, adjacent tissues, blood or secretions of patients, and measuring the expression and distribution of biological markers, the extent and source of cancer foci, the degree of differentiation of cancer cells, and the extent of invasion can be determine...
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57438G01N33/57484
Inventor 石国军潘序雅王何婷陈燕铭陈健宁温诗怡王瑨李婷艾鹤英何学敏朱延华
Owner THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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