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Anti-tumor combined preparation and application thereof

An anti-tumor and preparation technology, which is applied in the field of anti-tumor drugs, can solve problems such as poor therapeutic effects, and achieve the effects of reduced discomfort, good stability, and short preparation time

Inactive Publication Date: 2020-08-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] CN 110420197 A discloses a method for preparing drug-loaded ethosome microspheres that can target dendritic cells by electrostatic spraying method, which has certain restrictions on the treatment of tumors, and cancer immunotherapy must try to activate cell-mediated immunity , especially CD8 + Cytotoxic T lymphocytes (CTL), activated CD8 + T cells can directly kill malignant cells and can acquire a durable memory phenotype, thus preventing cancer recurrence, single-drug therapy is poor

Method used

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  • Anti-tumor combined preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0055] (1) Lecithin, cholesterol, and octadecylamine were mixed in 30% (v / v) ethanol aqueous solution at a mass ratio of 25:2.5:1, and rotary evaporation was used to obtain a layer of liposome membrane; the TRP2 peptide was mixed with 0.5 mg / ml was added with 30% (v / v) aqueous ethanol to obtain a TRP2 peptide solution; the liposome membrane was added to the TRP2 peptide solution, and stirred to obtain ethosomes loaded with TRP2 peptides; the ethosomes loaded with TRP2 peptides were dropped Adding it to the mannosylated polyethyleneimine solution, ultrasonically dispersing while stirring, and then adding immunoadjuvant (CPG-ODN) drugs to obtain the drug-loaded ethosome modified by mannosylated polyethyleneimine;

[0056] The preparation method of mannosylated polyethyleneimine is as follows: mixing 4-isosulfuric acid phenyl-A-D-mannoside and polyethyleneimine, adding methanol to initiate the reaction for 3 hours, then rotary evaporation, and freeze-drying for 3 days to obtain M...

Embodiment 2

[0061] (1) Divide 4-5 week male C57BL / 6 mice into 4 groups: blank control group without administration, PVP{TRP2@Eth-CpG@PEI man}-SF / PVA treatment group, aPD-1 treatment group, PVP{TRP2@Eth-CpG@PEI man}-SF / PVA+aPD-1 combined treatment group.

[0062] (2) The mice were anesthetized by intraperitoneal injection of 100uL of 1% pentobarbital solution, their abdominal hair was removed, and a small amount of PBS was used to moisten the skin. man}-SF / PVA) on the wetted area and fixed with medical tape for the first immunization. The second immunization was performed on day 14. aPD-1 was treated by subcutaneous injection, 20 μg / mouse.

[0063] (3) One week after the second immunization (three days for each immunization), each mouse was subcutaneously injected with 100uL density of 1×10 6 B16 cells in the right hind legs of mice. The mice were raised in a suitable environment, and the growth of the mouse tumor was observed every 3 days. After it grew to a suitable size, the tumor ...

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Abstract

The invention relates to an anti-tumor combined preparation and an application thereof. The preparation comprises a percutaneous immune membrane and an immune checkpoint blocking agent. According to the invention, the percutaneous tumor immune membrane is prepared mainly based on an alcohol plasma preparation technology and an electrostatic spraying technology, and an immune checkpoint blocker iscombined for antagonizing an immunosuppression pathway in T cells and enhancing CD8+ T cell mediated tumor destruction, so that long-term protection is provided, and tumor invasion, metastasis or recurrence is prevented.

Description

technical field [0001] The invention belongs to the field of antitumor drugs, in particular to an antitumor combined preparation and application thereof. Background technique [0002] The morbidity and mortality of malignant tumors are on the rise, which poses a great threat to human health and is one of the intractable diseases that the World Health Organization urgently needs to overcome. Malignant tumor is not a single disease, it is usually a local manifestation of systemic lesions, the possibility of metastasis and spread exists at any time, its development is a multi-step, multi-factor process. Therefore, the combination of multiple disciplines is required for its treatment, and the advantages of each treatment method should be brought into play, and the rational and scientific arrangement and implementation can achieve the best treatment effect. Among them, immunotherapy uses the patient's own immune system instead of cytotoxic chemotherapy drugs to kill tumor cells,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K9/70A61K47/32A61K47/34A61K47/42A61K39/00A61K39/39A61K39/395A61P35/00
CPCA61K9/7007A61K39/39A61K39/39558A61K45/06A61K47/32A61K47/34A61K47/42A61K2039/54A61K2039/55561A61P35/00A61K39/001156A61K2300/00
Inventor 王红声宋新然洪火炎
Owner DONGHUA UNIV
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