Production of monascus-like azaphilone pigment

A technology of Monascus and pigments, applied in the field of colorants, can solve problems such as instability to light, and achieve the effect of increasing light stability

Inactive Publication Date: 2011-02-09
DTU丹麦科技大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another drawback of the pigments produced by Monascus species is that they are notably unstable to light

Method used

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  • Production of monascus-like azaphilone pigment
  • Production of monascus-like azaphilone pigment
  • Production of monascus-like azaphilone pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0181] The selection of embodiment 1. fungus, culture medium and culture condition

[0182] All fungal isolates used in this study were produced from Biocentrum-DTU, Technical University of Denmark, Kgs. Lyngby, Denmark. Fungal isolates are listed by IBT number. All fungi were grown on any of four different solid media, Yeast Extract Sucrose (YES) Agar; Malt Extract Agar (MEA), Potato Dextrose (PD) Agar and Czapek-Dox Yeast Autolysate (CYA) agar (Frisvad, J.C.; Thrane, U. Mycological media for food-and indoor fungi. See Introduction to Food-and Airborne Fungi. Introduction), 6th ed.; Samson, R.A., Hoekstra, E.S., Frisvad, J.C., Fitenborg, O., eds.; Royal Netherlands Biotechnology Institute Culture Collection, Utrecht, The Netherlands, 2002; p378), or Cultivation in a specific combination of media on which it was found produces the largest pigments with interesting shades in the red to yellow spectral range. The cultures were grown in the dark at 25°C for 7 days.

[0183] P...

Embodiment 2

[0184] Example 2. Extraction of fungal pigments

[0185] Extraction was performed by a modified form of Smedsgaard's micro-extraction method (J. Chromatogr.A 1997, 760, 264-270), in which 6 mm plugs were extracted for 30 minutes in two steps in 2 ml vials, first using 1 ml containing 0.5 % formic acid in ethyl acetate to break down cell walls and extract relatively nonpolar metabolites. The extract thus obtained was then transferred to a new 2 ml vial and evaporated under vacuum. Based on our initial results showing maximal pigment extraction from specific pigment-producing fungi, a second extraction was performed using 1 ml of methanol or isopropanol. Because the extract chemistry of pigments varies from fungus to fungus, an appropriate solvent needs to be used for a particular strain. By doing this, we can extract maximum pigment. However, the same solvent system was used to extract the same strains grown in different media. A second extract was then added to the vial al...

Embodiment 3

[0187] Example 3. Analysis of pigments

[0188] 3.1 Colorimetric method: Adjust the absorbance value of the pigments in the filtered fermentation broth at the respective maximum absorption points with pure water as a diluent, which is obtained from a Milli-Q system (Millipore, Bedford, MA) in order to measure the Beer-Lambert's law The absorbance within the linearity of . The dilution factor was then taken into account to calculate yields based on volumetric production of Absorbance Units (AU) per 100 ml of broth. The absorbance maximum was determined by scanning the absorbance spectrum of the extract in the range of 350-700 nm using a spectrophotometer (Agilent HP 8453, Agilent technologies, Palo Alto, USA).

[0189] Absorbance is also used to determine color quality. The absorbance values ​​recorded at the pigment absorption maxima are two characteristic absorption peaks in the visible range of the spectrum, the first at approximately 495 nm and the other in the 407-420 nm...

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Abstract

The present invention relates to the field of biotechnological production of polyketide based colorants from filamentous fungi, in particular a method for preparing a biomass comprising a Monascus-like pigment composition from a nontoxigenic and non-pathogenic fungal source. The present invention further relates to use of the Monascus-like pigment composition as a colouring agent for food items and / or non-food items, and a cosmetic composition comprising the Monascus-like pigment composition.

Description

[0001] technical field of invention [0002] The present invention relates to the field of biotechnological production of polyketide-based colorants from filamentous fungi, in particular methods for the preparation of Monascus-like pigment compositions from non-toxic and non-pathogenic fungal sources. The present invention further relates to the use of the Monascus-like pigment composition as a colorant in food and / or non-food and cosmetic compositions. Background of the invention [0003] Currently, about 43 colorants have been approved as food additives in the European Union, while about 30 color additives have been approved for food use in the United States, and several of the listed color additives are derived from Natural source. [0004] Existing approved natural food colorants are mostly of plant origin and suffer from many drawbacks, such as chemical instability and low water solubility. For example, betaine, carotenoids, and chlorophyll pigments contain unstable hyd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00A23L1/275C12M1/16C12M1/06
CPCC09B61/00A23L2/58A23C19/0925A23C19/0682A23L1/2755C12P37/00C12P17/181A23L5/46
Inventor S·A·S·玛帕里A·S·梅尔J·C·弗里斯瓦德U·斯兰
Owner DTU丹麦科技大学
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