Tissue culture method of Hemerocallis dumortieri

A technology of Hemerocallis Hemerocallis and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of few introductions, slow ramets, limited supply of seedlings, etc., and improves the uniformity of seedlings. , The effect of maintaining maternal traits and increasing reproduction speed

Inactive Publication Date: 2010-10-27
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a variety imported from abroad, the number of introductions of thi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Obtaining sterile materials

[0026] Pick the buds of "Jinhuang" Evergreen Daylilies when they are blooming, rinse them with tap water for 1 hour, and then soak them on the ultra-clean workbench for 10 seconds in ethanol with a mass concentration of 60% and mercury with a volume concentration of 0.5‰ After 10 minutes, rinse with sterile water for 4 times, use sterile filter paper to absorb the water on the surface of the flower bud, cut the base of the flower bud into 0.5 cm long segments, and inoculate it with MS+KT0.1mg / L+NAA1.0mg / L flower bud induction medium;

[0027] (2) Differentiation and proliferation of buds

[0028] The flower buds were inoculated on the flower bud induction medium. After 1 week, they began to swell, and yellow-green protrusions appeared. After 3 weeks, the callus was visible. After another month of cultivation, the callus was divided, cut and connected to MS+6 -BA0.5mg / L+NAA0.1mg / L adventitious bud proliferation medium for proliferation and c...

Embodiment 2

[0037] (1) Obtaining sterile materials

[0038] Pick the buds of "Jinhuang" Evergreen Daylily when it blooms, rinse with tap water for 2 hours, and then soak it on the ultra-clean workbench for 30s in ethanol with a mass concentration of 75% and mercury with a volume concentration of 1‰ After 15 minutes, rinse with sterile water for 5 times, use sterile filter paper to absorb the water on the surface of the flower buds, cut the base of the flower buds into 1 cm long segments, and inoculate them with MS+KT0.5mg / L+NAA3.0mg / L On the bud induction medium;

[0039] (2) Differentiation and proliferation of buds

[0040] The flower buds were inoculated on the flower bud induction medium. After 2 weeks, they began to expand and appeared yellow-green protrusions. After 3 weeks, the callus could be seen. After another month of cultivation, the callus was divided and cut and connected to MS+6. -BA2.0mg / L+NAA0.2mg / L adventitious bud proliferation medium for proliferation and culture. Although ...

Embodiment 3

[0049] (1) Obtaining sterile materials

[0050] Pick the buds of "Jinhuang" Evergreen Hemerocallis when it is blooming, rinse with tap water for 3 hours, and then soak it on the ultra-clean workbench for 50s in ethanol with a mass concentration of 75% and mercury with a volume concentration of 2‰ After 30 minutes, rinse with sterile water for 6 times, use sterile filter paper to absorb the water on the surface of the flower buds, cut the base of the flower buds into 2cm-long segments, and inoculate them with MS+KT1.0mg / L+NAA5.0mg / L On the bud induction medium;

[0051] (2) Differentiation and proliferation of buds

[0052] The flower buds were inoculated on the flower bud induction medium. After 3 weeks, they began to swell and appeared yellow-green protrusions. After 5 weeks, the callus could be seen. After another 2 months, the callus was divided and cut and connected to MS+6. -BA4.0mg / L+NAA0.2mg / L adventitious bud proliferation medium for proliferation and culture. Although ther...

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PUM

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Abstract

The invention relates to a tissue culture method of Hemerocallis dumortieri, comprising the steps of: acquisition of bacteria-free material, differentiation and proliferation of bud, sound seeding of adventitious bud, rooting culture, hardening and transplantation and the like. Compared with the prior art, the tissue culture method greatly enhances reproduction speed of Hemerocallis dumortieri and uniformity of seedlings, better maintains original maternal character, and can realize batch production of seedlings in factory.

Description

Technical field [0001] The invention relates to a method for tissue culture of plants, in particular to a method for tissue culture of the evergreen Hemerocallis grandiflora. Background technique [0002] "Jinhuang" Evergreen Daylily is a plant of the Liliaceae Hemerocallis. It is native to the Yangtze River basin in Japan from southern Europe through northern Asia to Japan. It is a perennial evergreen plant with short rhizomes and thick spindle-shaped fleshy roots. The leaves are basal. , Wide linear, tender green. It blooms in early summer, the flowers are large, funnel-shaped, about 10cm in diameter, and the perianth lobes have multiple oblong petals and are golden yellow. The blooming period of "Jinhuang" large evergreen daylily is from early June to mid-July. The flower color is bright, easy to cultivate, and it is evergreen with clumps of green leaves, which is very beautiful. Many clumps are planted in gardens or in flower borders and roads. beside. "Jinhuang" evergreen...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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