Compound inoculant for improvement of saline-alkali soil and preparation and application of same
A compound bacterial agent, saline-alkali soil technology, applied in application, fungicide, biocide and other directions, can solve the problems of low saturated hydraulic conductivity of saline-alkali soil, affecting plant nutrient absorption, reducing protein activity, etc., to achieve uniform coloring effect.
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[0090] 1. Preparation of bacteria on solid slant
[0091] The preserved strains are inoculated on slant culture medium, and cultured statically at a constant temperature of 30°C to 37°C for 24h to 72h. Microscopic examination shows no bacteria and the spore rate is greater than 90%, and they are stored in a refrigerator at 4°C. Use within 30 days.
[0092] The composition of described slant medium:
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[0095] 2. Preparation of bottled liquid strains
[0096] Under sterile conditions, the 2cm 2 The slant solid culture is inoculated into 100ml shake flask seed medium, and shake cultured at 180rpm-200rpm, 30°C-37°C for 20h-72h at a constant temperature to obtain shake flask seed liquid.
[0097] The shake flask seed medium
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[0099]
[0100] Described shaking flask seed culture condition and shaking bottle seed liquid standard
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