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Compound inoculant for improvement of saline-alkali soil and preparation and application of same

A compound bacterial agent, saline-alkali soil technology, applied in application, fungicide, biocide and other directions, can solve the problems of low saturated hydraulic conductivity of saline-alkali soil, affecting plant nutrient absorption, reducing protein activity, etc., to achieve uniform coloring effect.

Inactive Publication Date: 2017-02-01
北京康缘益生生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the formation of Na + —Protein, which reduces the activity of protein and affects the nutrient absorption of plants
[0008] 2. High salt content
[0009] The soluble salt content is high, which increases the osmotic pressure of the soil solution, and the physiological drought of plants reduces the efficiency of water, thereby inhibiting the normal growth of plants
[0010] At the same time, if the concentration of a certain salt is too high, it will cause mono-salt poisoning to crops.
[0011] 3. Poor soil structure
[0013] At the same time, the saturated hydraulic conductivity of saline-alkali soil is low, which affects the leaching effect of salt
[0014] In short, due to the above characteristics of saline-alkali soil, it will cause certain harm to the growth of crop root system, the formation of cell membrane structure, the opening and closing of stomata, the absorption of nutrients and genetic material, etc., which reduces the stress resistance of crops, and then affects the normal operation of crops. to grow

Method used

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  • Compound inoculant for improvement of saline-alkali soil and preparation and application of same
  • Compound inoculant for improvement of saline-alkali soil and preparation and application of same
  • Compound inoculant for improvement of saline-alkali soil and preparation and application of same

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preparation example Construction

[0090] 1. Preparation of bacteria on solid slant

[0091] The preserved strains are inoculated on slant culture medium, and cultured statically at a constant temperature of 30°C to 37°C for 24h to 72h. Microscopic examination shows no bacteria and the spore rate is greater than 90%, and they are stored in a refrigerator at 4°C. Use within 30 days.

[0092] The composition of described slant medium:

[0093]

[0094]

[0095] 2. Preparation of bottled liquid strains

[0096] Under sterile conditions, the 2cm 2 The slant solid culture is inoculated into 100ml shake flask seed medium, and shake cultured at 180rpm-200rpm, 30°C-37°C for 20h-72h at a constant temperature to obtain shake flask seed liquid.

[0097] The shake flask seed medium

[0098]

[0099]

[0100] Described shaking flask seed culture condition and shaking bottle seed liquid standard

[0101]

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Abstract

The invention relates to a compound inoculant for improvement of saline-alkali soil and particularly provides a novel compound inoculant for improvement of saline-alkali soil, and preparation and applications of same. Bacillus amyloliquefaciens, bacillus megatherium, bacillus mucilaginosus and paenibacillus azotofixans are fermented respectively to obtain fermentation liquids, which are then mixed with a carrier respectively according to certain volume-weight ratio (V / kg); the mixtures are dried at low temperature and finally are compounded according to ratio. The compound inoculant is a powder agent, wherein the microorganisms therein are functional bacteria, and the inoculant is high in living bacteria number and especially contains high-activity metabolic products, so that the compound inoculant is suitable for being mixed with a base fertilizer, especially high-quality farmyard manure, for use. When being applied in soil, high environment adaption capability and high reproduction speed are achieved. The compound inoculant can increase beneficial microbial flora in rhizosphere and rapidly decompose organic substances for absorption and utilization by plants, can reduce the alkalinity of soil, improve fertility of soil and improve the water permeability and retention and breathability properties of the soil, and can accelerate growth of root system and prevent spreading of soil-borne diseases, thereby maintaining ecological balance, reducing cost and improving the saline-alkali soil.

Description

technical field [0001] The invention relates to a compound microbial agent for improving saline-alkali soil, in particular to a novel compound bacterial agent for improving saline-alkali soil and its preparation and application. Background technique [0002] Saline-alkali land is a type of salt accumulation, which means that the salt contained in the soil affects the normal growth of crops. According to the incomplete statistics of UNESCO and FAO, the area of ​​saline-alkali land in the world is 954.38 million hectares, of which my country is the largest 99.13 million hectares. The formation of alkaline soil and alkalized soil in my country is mostly related to the accumulation of carbonate in the soil, so the degree of alkalization is generally high, and plants in severe saline-alkali soil areas can hardly survive. [0003] Main characteristics of saline-alkali land and its harm to crops [0004] 1. High salinity [0005] The pH of saline is usually alkaline. When Na + ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40A01N63/02A01P1/00A01P3/00C09K101/00
CPCA01N63/10C09K17/40C09K2101/00
Inventor 张传军张晓红张中亲张宪德张亚洁刘长青白玉红李冰洁
Owner 北京康缘益生生物科技有限公司
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