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Method for promoting expression of gene CS of siraitia grosvenorii

A technology of gene expression and Luo Han Guo, which is applied in the field of promoting the expression of Luo Han Guo CS gene, can solve the problem of low production of Mogroside V and the like, and achieve the effect of promoting expression

Inactive Publication Date: 2017-11-07
韦荣昌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] An object of the present invention is to solve the problem of low yield of mogroside V cultivated by traditional methods, and to provide at least the advantages that will be described later

Method used

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  • Method for promoting expression of gene CS of siraitia grosvenorii

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Embodiment 1

[0022] A method for promoting the expression of the CS gene of Luo Han Guo. The tissue cultured seedlings of Luo Han Guo are inoculated in the induction medium containing methyl jasmonate with a concentration of 50 μmol / L, placed in a humidity of 60%, light intensity of 1200 lux, light time of 8 h / d, and temperature Cultivate for 30 days at 23±2°C. The solid medium components are: MS+6-BA 1.5mg / L+indole butyric acid 0.3mg / L+agar 3.5g / L+sucrose 30g / l+activated carbon 1.0g / L; the method of adding methyl jasmonate is: Dissolve methyl jasmonate in aqueous ethanol solution with a volume fraction of 2% to prepare methyl jasmonate mother liquor, and sterilize with a 0.22 μm microporous membrane, cool to 24-26 °C after sterilization, and then cool the methyl jasmonate The ester mother solution was added to the above solid medium until the final concentration of methyl jasmonate was 50 μmol / L to obtain an induction medium.

Embodiment 2

[0024] A method for promoting the expression of the CS gene of Luo Han Guo. The tissue cultured seedlings of Luo Han Guo are inoculated in the induction medium containing methyl jasmonate with a concentration of 135 μmol / L, placed in a humidity of 65%, light intensity of 1400 lux, light time of 8 h / d, and temperature Cultivate for 30 days at 23±2°C. The solid medium components are: MS+6-BA 1.5mg / L+indole butyric acid 0.3mg / L+agar 3.5g / L+sucrose 30g / l+activated carbon 1.0g / L; the method of adding methyl jasmonate is: Dissolve methyl jasmonate in aqueous ethanol solution with a volume fraction of 2% to prepare methyl jasmonate mother liquor, and sterilize with a 0.22 μm microporous membrane, cool to 24-26 °C after sterilization, and then cool the methyl jasmonate The ester mother solution was added to the above solid medium until the final concentration of methyl jasmonate was 135 μmol / L to obtain an induction medium.

Embodiment 3

[0026] A method for promoting the expression of the CS gene of Luo Han Guo. The tissue cultured seedlings of Luo Han Guo are inoculated in the induction medium containing methyl jasmonate with a concentration of 175 μmol / L, placed in a humidity of 66%, light intensity of 1400 lux, light time of 8 h / d, and temperature Cultivate for 30 days at 23±2°C. The solid medium components are: MS+6-BA 1.5mg / L+indole butyric acid 0.3mg / L+agar 3.5g / L+sucrose 30g / l+activated carbon 1.0g / L; the method of adding methyl jasmonate is: Dissolve methyl jasmonate in aqueous ethanol solution with a volume fraction of 2% to prepare methyl jasmonate mother liquor, and sterilize with a 0.22 μm microporous membrane, cool to 24-26 °C after sterilization, and then cool the methyl jasmonate The ester mother solution was added to the above solid medium until the final concentration of methyl jasmonate was 175 μmol / L to obtain an induction medium.

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Abstract

The invention discloses a method for promoting the expression of a gene CS of siraitia grosvenorii. The method is characterized in that tissue-cultured seedlings of the siraitia grosvenorii are inoculated in an inducing medium containing methyl jasmonate with concentration of 50-175mu mol / L and are cultured for 28-30d. According to the method, the methyl jasmonate is added into the medium for tissue-cultured seedlings of the siraitia grosvenorii, so that the methyl jasmonate acts on the tissue-cultured seedlings all the time in the growth process of the siraitia grosvenorii tissue-cultured seedlings, the expression of the gene CS in the tissue-cultured seedlings of the siraitia grosvenorii is induced, and the synthesis of mogroside V is further promoted.

Description

technical field [0001] The invention relates to the field of plant cultivation. More specifically, the present invention relates to a method for promoting the expression of the CS gene of Luo Han Guo. Background technique [0002] Mogroside V is extracted from the specialty economic plant in Guangxi—Mogros grosvenori. Its sweetness is 300 times that of sucrose, and its calories are zero. Prevention and control effect, the current market demand for mogroside V is large, so it is necessary to improve the planting efficiency to increase the output of mogroside V to meet the needs of the market. Mogroside V belongs to the cucurbitane-type tetracyclic triterpenoids. Our research group has deduced the possible biosynthetic pathway of Mogroside V based on the transcriptome data of Luo Han Guo. The precursors for the biosynthesis of mogroside V are isopentenyl diphosphate (IPP) and 3,3-dimethylenylpyrophosphate (DMAPP), both of which are produced by mevalonate (MVA) and methyl Tw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/06
CPCA01H4/008A01G7/06A01H4/001
Inventor 韦荣昌黄小华
Owner 韦荣昌
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