The invention discloses a self-stabilizing conducting comb signal source. The signal source comprises a shielding outer shell, a signal wire and a plug that are externally connected, and a charging protection module, a rechargeable battery and a circuit board that are built-in, wherein the circuit board comprises a boosting voltage-stabilizing circuit, a linear voltage-stabilizing circuit, a crystal oscillating circuit, a fractional frequency choosing circuit, a fractional frequency shaping circuit, a stepped shaping circuit and a balance output circuit; a charging power source charges the rechargeable battery through the charging protection module; and the rechargeable battery supplies power to the circuit board so as to form a comb spectral line through the circuit board and output the signal through the plug. The self-stabilizing conducting comb signal source has the advantages of simple structure, convenient operation and stable property, is mainly used for checking the accuracy of an electromagnetic interference test system or periodically checking the device in the laboratory, and has a function of standard substance.