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Mesenchymal stem cell injection and preparation method thereof as well as application in preparation of medicine for treating ulcerative colitis

A technology for ulcerative colitis and stem cells, applied in the field of biomedicine, can solve serious problems, toxic side effects, anastomotic bleeding and other problems, and achieve the effect of preventing continued destruction, getting rid of toxic side effects, and being easy to industrialize.

Inactive Publication Date: 2013-02-13
青岛奥克生物开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Disadvantages of the existing technology: the current treatment of ulcerative colitis mainly depends on diet control, sulfasalazine and hormones, traditional Chinese medicine enema and other drug treatments, and surgical treatment is required in severe cases
The comprehensive application of these therapies can temporarily control symptoms such as abdominal pain, diarrhea and pus and blood in the stool, but cannot effectively correct the immune disorder in the patient's body, and cannot fundamentally treat ulcerative colitis and prevent the progression and recurrence of the disease
Moreover, sulfasalazine and hormones and other drugs have very serious side effects, such as: allergic reaction, neutropenia or deficiency, thrombocytopenia and aplastic anemia, hemolytic anemia and hemoglobinuria, liver damage, etc.
Surgery can partially cure the disease, but there are many and serious postoperative complications, such as adhesive intestinal obstruction, anastomotic bleeding, fistula, etc., which seriously affect the quality of life of patients

Method used

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  • Mesenchymal stem cell injection and preparation method thereof as well as application in preparation of medicine for treating ulcerative colitis
  • Mesenchymal stem cell injection and preparation method thereof as well as application in preparation of medicine for treating ulcerative colitis
  • Mesenchymal stem cell injection and preparation method thereof as well as application in preparation of medicine for treating ulcerative colitis

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Experimental program
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Effect test

Embodiment 1

[0032] 1. Preparation of umbilical cord mesenchymal stem cells

[0033] 1. The umbilical cord of a fresh full-term healthy fetus is washed with PBS buffer, which contains 100 kU / L penicillin and 100 mg / L streptomycin;

[0034] 2. Cut the 5-10cm long umbilical cord, cut the umbilical cord into 2cm long pieces, and wash it repeatedly with PBS buffer; the PBS buffer contains 100 kU / L penicillin and 100 mg / L streptomycin;

[0035] 3. Cut the umbilical cord tissue pieces into 2-5mm pieces 3 Small pieces; add L-DMEM medium to wash, centrifuge at 500-800g for 5 minutes, and discard the supernatant;

[0036] 4. Add the tissue block and medium to the medium at a volume ratio of 2.5-3:1, mix the tissue block, inoculate it into a cell culture dish, and culture it in an incubator; the medium contains 1-10 ng / ml Basic fibroblast growth factor (bFGF) and serum-free medium for MSC;

[0037] 5. Change the medium every 3 days, and subculture when the cells reach about 80% confluence in 8-9 ...

Embodiment 2

[0071] For example, prepare 100ml of stem cell injection, which consists of human mesenchymal stem cells, 10ml of clinical grade DMSO, 10ml of human serum albumin stock solution (concentration of the stock solution is 20%), 1ml of low molecular weight dextran and 79ml of Bomaili (compound electrolyte solution) . The number of mesenchymal stem cells per milliliter injection is 2×10 5 -1×10 7 . Mesenchymal stem cell injection can still maintain a single-cell suspension state at an ambient temperature of -196°C, and the cell viability remains above 85% after resuscitation, which can be directly used for clinical injection.

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Abstract

The invention discloses a mesenchymal stem cell injection and a preparation method of the mesenchymal stem cell injection and an application in preparation of a medicine for treating ulcerative colitis, wherein the mesenchymal stem cell injection consists of the following components: 2*105-1*107 pieces / ml of mesenchymal stem cells, 5-10% of dimethyl sulfoxide (DMSO) by volume, 1-6% of human albumin by mass-to-volume ratio, and 1% of low molecular dextran and compound electrolyte solution. According to the mesenchymal stem cell injection, the preparation method and the application of the mesenchymal stem cell injection in preparation of the medicine for treating ulcerative colitis, the mesenchymal stem cell injection is used for correcting immune dysfunction of a patient of ulcerative colitis, and preventing the autoimmune dysfunction from continuously damaging the intestinal tract; meanwhile, many cell growth factors and repair factors are secreted, to promote pathologically changed intestinal ulcer to heal, therefore, the purpose of foundational treating the ulcerative colitis is achieved. The disease course of the ulcerative colitis can be reversed, the patient can be helped in escaping out of influence of stomachache and diarrhea on the life as well as toxic and side effects of the medicine, so as to treat the ulcerative colitis thoroughly.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a mesenchymal stem cell injection, a preparation method thereof, and an application in preparation of medicines for treating ulcerative colitis. Background technique [0002] Ulcerative colitis can occur at any age But more common in 20-40 years old. Developed countries and more cities than rural areas The cause of the disease is still unclear, but it is currently considered to be an immune disorder, and genetic and environmental factors work together to cause the disease. Ulcerative colitis predisposes to certain families, study finds About 20% of people with ulcerative colitis have a first-degree relative (ie, cousin / sister or closer) who also has ulcerative colitis, so genetics play a role. Except for gastrointestinal symptoms Some people have symptoms elsewhere in the body, including: red and itchy eyes; mouth sores; joint swelling and pain; skin bumps and othe...

Claims

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Application Information

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IPC IPC(8): A61K35/44A61K35/50A61K47/42C12N5/0775A61P1/04
CPCA61K35/50A61K35/44A61K47/42C12N5/0665A61P1/04
Inventor 魏斯溧高宏王丽胡建霞张学峰
Owner 青岛奥克生物开发有限公司
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