Patents
Literature
Patsnap Copilot is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Patsnap Copilot

106results about How to "Easy quality control" patented technology

Mesenchymal stem cell injection, preparation method and application thereof in preparing medicine for treating diabetes

The invention discloses a mesenchymal stem cell injection, a preparation method and application of the mesenchymal stem cell injection in preparing a medicine for treating diabetes; the mesenchymal stem cells are derived from human umbilical cord and placenta; the mesenchymal stem cell injection consists of components: human mesenchymal stem cells, human albumin, low molecular heparin calcium, compound amino acid, vitamin C of 0.5% and dissolving medium; and the dissolving medium can be a compound electrolyte solution or glucose or normal saline. According to the mesenchymal stem cell injection, the preparation method and the application of the mesenchymal stem cell injection in preparing the medicine for treating the diabetes, the mesenchymal stem cell injection is used for repairing injured islet beta cells, and the blood sugar is reduced by secretion of the endogenous insulin, therefore, a purpose of foundational treating the diabetes is achieved. A disease course of the diabetes can be reversed, patients are helped in escaping out of inconvenience and toxic and side effects of taking the endogenous drug and injecting the insulin as well as serious complications caused by poor control of the blood sugar; and the 1-type and 2-type diabetes are treated thoroughly.
Owner:青岛奥克生物开发有限公司

Near infrared quality control analysis method and system of tobacco

The invention discloses a near infrared quality control analysis method of tobacco. The method comprises the following steps of: collecting representative tobacco samples and grinding into powder; opening a tobacco sample acquisition instrument and preheating; setting parameters of the instrument; scanning the representative tobacco samples one by one; selecting an unknown sample, grinding into powder and forming a scanner spectrogram; opening a chemometric module of an analysis system, selecting an algorithm, preprocessing spectral data and establishing a correction model: setting a wavelength range of between 700 and 1,100nm, standardizing a spectrum value and chemical value, saving the established model and starting to correct the model; and predicting the sample and starting to calculate after selecting the unknown sample so as to obtain a quantitative and qualitative sample analysis result. Through the method and the system, the quality parameter of the tobacco can be quickly measured, and the quality of the tobacco can be quickly measured, controlled and analyzed on site. The method and the system are also suitable for quality monitoring of medicaments, milk, milk powder and the like, raw material comparison, analysis of product consistency and the like.
Owner:BEIJING KAIYUAN SHENGSHI SCI & TECH DEV

Preparation method and application of classical swine fever virus recombinant subunit vaccine

The invention discloses a preparation method and application of a classical swine fever virus recombinant subunit vaccine with the amino acid sequence shown as SEQ ID No.1. The preparation method of the classical swine fever virus recombinant subunit vaccine typically includes the following steps: classical swine fever E2 truncated protein (TE2) coding gene is cloned into baculovirus vector pFastBacTM1, and is then transfected into Sf9 insect cells to obtain recombinant baculovirus capable of expressing protein TE2. The high five insect cells in logarithmic growth phase are infected by the recombinant baculovirus, so that a large amount of the protein TE2 can be expressed in a cell culture supernatant. Finally, the cell culture supernatant is recovered and purified to obtain a large amount of the recombinant protein TE2 with the purity more than 90%. According to the method, the target protein can be harvested from the cell culture supernatant, the time of protein purification is reduced, consumption of a large amount of time can be avoided, and the vaccine production process can be simplified. Under the premise of simplification of the vaccine production process, the recombinant protein TE2 has the advantages of strong immunogenicity and high safety, and the animal experiments prove that the recombinant protein can effectively stimulate the body to produce a highly effective humoral immune response.
Owner:NOVO BIOTECH CORP

Aluminium/resin composite exhibiting excellent weather resistance and manufacturing method for the same

Disclosed is an aluminium/resin composite which: exhibits extremely high interfacial adhesion strength and air tightness between an integrally-bonded, shaped aluminium article made from an aluminium alloy, and a molded resin article; retains the excellent adhesion strength and air tightness of the aluminium/resin bond surface in harsh environments such as those caused by temperature, humidity, dust, and corrosive materials; and is corrosion resistant, heat resistant, weather resistant, durable, and exhibits excellent peel strength on the aluminium/resin interfacial surface. Also disclosed is a manufacturing method for the aluminium/resin composite. The aluminium/resin composite contains a shaped aluminium article having undulations on the surface, and a molded resin article which is bonded to the shaped aluminium article when the resin is butted thereto. A plurality of concave sections are formed due to the undulations, and an aluminium coating film is formed on the outermost surface of the undulations. Fitting sections are formed on the molded resin article where the resin enters the concave sections and hardens. The components of the aluminium/resin composite which exhibits excellent weather resistance are engaged by the concave sections and the fitting sections.
Owner:NIPPON LIGHT METAL CO LTD

Recombinant African swine fever virus CD2V subunit protein as well as preparation method and application thereof

The invention discloses a recombinant African swine fever virus CD2V subunit protein as well as a preparation method and application thereof. The protein comprises an extracellular region and an intracellular region of African swine fever virus surface envelope protein, and the amino acid sequence of the protein is shown as SEQ ID NO.3. The preparation method comprises the following steps: 1) cloning a codon-optimized gene sequence shown as SEQ ID NO.1 into an eukaryotic expression vector; 2) transfecting a recombinant expression vector containing the African swine fever virus subunit proteincoding gene into CHO cells; 3) culturing, screening and domesticating a CHO cell strain in the step 2) to obtain a highly-expressed cell strain; 4) fermenting and culturing the cell strain in the step3), and performing purifying to obtain the African swine fever virus CD2V subunit protein; and 5) mixing the CD2V protein with a pharmaceutically acceptable adjuvant to obtain a subunit vaccine. Theinvention can provide the African swine fever surface CD2V subunit protein which can be industrially produced on a large scale, the preparation method is simple and low in cost, and the prepared vaccine can reach the existing national standard.
Owner:NOVO BIOTECH CORP

Basement exterior wall single-side formwork supporting system and method

The invention discloses a basement exterior wall single-side formwork supporting system and method and relates to the technical field of building construction. The basement exterior wall single-side formwork supporting system and method are disclosed to solve the problems that a traditional basement exterior wall single-side formwork supporting system is low in supporting efficiency and the supporting quality is hard to guarantee. According to the formwork supporting system, a reinforcing rod, a batten, a formwork and a fender post are fixedly connected in sequence along a basement exterior wall from inside to outside through split bolts, and inclined struts are arranged between the side, away from the batten, of the reinforcing rod and a foundation slab. The formwork supporting method comprises the steps that firstly, a waterproof steel plate is arranged; secondly, the reinforcing rod, the batten and the formwork are fixedly connected to form the formwork supporting system through the split bolts, the lower end of the formwork supporting system is fixedly connected with steel plates pre-buried in the foundation slab, the upper end of the formwork supporting system is fixedly connected with a concrete beam through angle steel and anchor bars, and the inclined struts are installed; and thirdly, joints and the formwork supporting system are fixedly connected through waterproof split bolts.
Owner:SHANGHAI CONSTR NO 1 GRP

Novel genetic engineering subunit vaccine for avian Newcastle disease viruses

The invention provides a novel genetic engineering subunit vaccine for avian Newcastle disease viruses. The preparation method for the vaccine comprises the following steps: optimizing coding genes ofHN protein and F protein of the avian Newcastle disease viruses (NDV); respectively cloning eukaryotic expression vectors containing optimized HN and F protein coding genes, respectively transfectingCHO cells, screening CHO cell strains capable of stably and efficiently expressing recombinant HN and F proteins in a suspension manner, culturing the CHO cell strains, and carrying out separation soas to obtain recombinant HN and F proteins; and fully and uniformly mixing the recombinant HN protein, the recombinant F protein and an adjuvant. According to the invention, coding genes of importantantigen proteins HN protein and F protein of NDV are optimized; CHO cells are used for eukaryotic expression; protein glycosylation is sufficient; antigen protein immunogenicity is good; safety is high; the expression quantity can reach 2-4 g / L; meanwhile, recombinant cells can be subjected to large-scale suspension culture; the complex degree of vaccine preparation is greatly reduced; the process controllability is good; the quality of products between batches is stable; and the production cost is remarkably reduced.
Owner:苏州沃美生物有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products