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Mesenchymal stem cell injection, preparation method and application thereof in preparing medicine for treating diabetes

A technology of diabetes medicine and mesenchymal stem cells, which is applied in the field of biomedicine, can solve the problems of not fundamentally treating diabetes, not effectively repairing islet function, liver and kidney damage, etc., and achieving the effect of treating diabetes, lowering blood sugar and restoring islet function

Inactive Publication Date: 2013-02-13
青岛奥克生物开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current drugs and insulin therapy cannot avoid these situations very well. Repairing and regenerating pancreatic β cells, restoring endogenous insulin secretion, and self-control of blood sugar are the fundamentals of treating diabetes
[0009] Shortcomings of the prior art: the current treatment of diabetes mainly relies on diet control, exercise therapy, drug and insulin comprehensive treatment
The comprehensive application of these therapies can control blood sugar and stabilize it in a short period of time, but only relying on external drugs to lower blood sugar cannot effectively repair the damaged islet function, and cannot fundamentally treat diabetes and prevent the progression of the disease
And many hypoglycemic drugs have very serious side effects, such as: severe hypoglycemia, diarrhea, liver and kidney damage, etc.

Method used

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  • Mesenchymal stem cell injection, preparation method and application thereof in preparing medicine for treating diabetes
  • Mesenchymal stem cell injection, preparation method and application thereof in preparing medicine for treating diabetes
  • Mesenchymal stem cell injection, preparation method and application thereof in preparing medicine for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of umbilical cord mesenchymal stem cells:

[0027] 1. The umbilical cord of a fresh full-term healthy fetus is washed with PBS buffer, which contains 100 kU / L penicillin and 100 mg / L streptomycin;

[0028] 2. Cut the 6-8cm long umbilical cord, cut the umbilical cord into 2cm long pieces, and wash it repeatedly with PBS buffer;

[0029] 3. Cut the umbilical cord tissue pieces into 2-3mm pieces 3 Small piece;

[0030] 4. Add the chopped tissue to L-DMEM medium for washing, centrifuge at 500-700g for 5 minutes, and discard the supernatant;

[0031] 5. Add the tissue block and the culture medium to the medium at a volume ratio of 2.5-3:1, mix the tissue block, inoculate it into a cell culture dish, and culture it in an incubator; the medium contains 1-10 ng / ml Basic fibroblast growth factor (bFGF) and L-DMEM medium with volume percentage 10%-15% fetal bovine serum (FBS);

[0032] 6. After 24 hours, add the culture medium described in step 5;

[0033] 7. ...

Embodiment 2

[0064] The preparation of umbilical cord and placental mesenchymal stem cells was the same as in Example 1.

[0065] For example, to prepare 100ml of stem cell injection, the injection consists of 25ml of human serum albumin stock solution (final concentration of mass volume ratio is 5%), 0.5ml of low molecular weight heparin calcium (final concentration of mass volume ratio is 0.5%), 20ml of compound amino acid (mass volume ratio of 0.5%), The volume ratio final concentration is 20%), vitamin C 0.5g (mass volume ratio final concentration is 0.5%), 54ml of 5% glucose injection and mesenchymal stem cells. Except for mesenchymal stem cells, the remaining components of the injection should be prepared in advance and pre-cooled at 4°C for later use. Mesenchymal stem cells are finally resuspended in this solution to make a single-cell suspension. The number of mesenchymal stem cells per ml of injection 1×10 7 . Mesenchymal stem cells remain in a single-cell suspension state withi...

Embodiment 3

[0067] The preparation of umbilical cord and placental mesenchymal stem cells was the same as in Example 1.

[0068] For example, to prepare 100ml of stem cell injection, the injection consists of 10ml of human serum albumin stock solution (the final concentration of mass volume ratio is 2%), 0.5ml of low molecular weight heparin calcium (the final concentration of mass volume ratio is 0.5%), 10ml of compound amino acid (mass volume ratio The volume ratio final concentration is 10%), vitamin C 0.5g (mass volume ratio final concentration is 0.5%), 0.9% normal saline injection 79ml and mesenchymal stem cells. Except for mesenchymal stem cells, the remaining components of the injection should be prepared in advance and pre-cooled at 4°C for later use. The prepared injection should be used within 1 week. The mesenchymal stem cells are finally resuspended in this solution to make a single cell suspension, and the number of mesenchymal stem cells per milliliter of injection is 2×10 ...

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Abstract

The invention discloses a mesenchymal stem cell injection, a preparation method and application of the mesenchymal stem cell injection in preparing a medicine for treating diabetes; the mesenchymal stem cells are derived from human umbilical cord and placenta; the mesenchymal stem cell injection consists of components: human mesenchymal stem cells, human albumin, low molecular heparin calcium, compound amino acid, vitamin C of 0.5% and dissolving medium; and the dissolving medium can be a compound electrolyte solution or glucose or normal saline. According to the mesenchymal stem cell injection, the preparation method and the application of the mesenchymal stem cell injection in preparing the medicine for treating the diabetes, the mesenchymal stem cell injection is used for repairing injured islet beta cells, and the blood sugar is reduced by secretion of the endogenous insulin, therefore, a purpose of foundational treating the diabetes is achieved. A disease course of the diabetes can be reversed, patients are helped in escaping out of inconvenience and toxic and side effects of taking the endogenous drug and injecting the insulin as well as serious complications caused by poor control of the blood sugar; and the 1-type and 2-type diabetes are treated thoroughly.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a mesenchymal stem cell injection, a preparation method thereof and an application in preparation of a drug for treating diabetes. Background technique [0002] The incidence of diabetes is getting higher and higher. According to statistics, there are now 150 million diabetic patients in the world. Experts predict that it will reach 300 million by 2025, of which 75% are in developing countries such as India and China. Diabetes is on the rise among Chinese in the world. In mainland China, the incidence rate has risen from 1% more than ten years ago to 2.5~3.25% at present, and the incidence rate of diabetes in urban adults is close to 10%. [0003] Diabetes is mainly divided into type 1, type 2, gestational and other types of diabetes. Type 1 diabetes (T1DM) is characterized by pancreatic β-cell damage, and type 1A is a T-lymphocyte-mediated autoimmune disease. Under the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/44A61K35/50A61K47/42C12N5/0775A61P3/10A61K35/28A61K35/51
CPCA61K35/51A61K38/385A61K47/42A61K35/50C12N5/0665A61K35/28C12N5/0668C12N2509/10A61P3/10A61K2300/00C12N2500/90
Inventor 魏斯溧高宏王丽胡建霞张学峰
Owner 青岛奥克生物开发有限公司
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