Umbilical cord mesenchymal stem cell injection with anti-aging function and preparation method thereof

Abstract

The invention provides an umbilical cord mesenchymal stem cell injection with an anti-aging function and a preparation method thereof. The injection comprises the following components: umbilical cord mesenchymal stem cell (5*10<5> to 10*10<5> / mL), 2 wt% of human albumin, and 98 wt% of compound electrolyte solution. The produced umbilical cord mesenchymal stem cells can rapidly repair and replace damaged cells and tissues, and have the characteristics of amplification and directional cell differentiation; and after the stem cells receive the signals from damaged parts, the stem cells will move to the damage parts, amplify in the damage parts, carry out induced differentiation, promote the self recovery function of stem cells, rapidly repair and replace damaged cells and tissues, rapidly secrete cell factors, growth factors, and tissue related factors in time, thus improve the blood circulation, enhance the metabolism functions, improve the tissue repairing function and immunity, and finally achieve the anti-aging goal.

Description

technical field

[0001] The invention relates to an umbilical cord mesenchymal stem cell injection with anti-aging effect and a preparation method thereof, belonging to the technical field of cell biology. Background technique

[0002] Stem cells are primitive cells with self-replication and multi-directional differentiation potential, and are the origin cells that form various tissues and organs of living organisms. Stem cell technology is one of the most important technologies in the field of life science and technology. It can derive cells into various types of cells and their tissues, resulting in some new therapeutic technologies, such as stem cell transplantation, tissue and organ reconstruction, and gene therapy. People can use their own or others' healthy stem cells and their derived tissues and organs to replace diseased or aging tissues and organs.

[0003] According to their developmental stage, stem cells can be divided into embryonic stem cells (Embryonic stem c...

Examples

Embodiment 1

[0050] This embodiment provides a method for extracting umbilical cord mesenchymal stem cells, which specifically includes the following steps:

[0051] Step 1. Screening and preservation of umbilical cords: screening and collecting umbilical cords of healthy full-term maternal volunteers, placing them in umbilical cord preservation solution at 4°C, and separating them within 24 hours; the umbilical cord preservation solution is a serum-free medium, without Contain antibiotics; the detection items of the screening include carrying virus, genetic family history, etc.; the viruses such as hepatitis B, hepatitis C, AIDS, syphilis, etc.;

[0052] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0053] Step 2. Separation of the umbilical cord: transfer the umbilical cord to a Petri dish and wash it with normal saline; remov...

Embodiment 2

[0060] This implementation provides a method for preparing an umbilical cord mesenchymal stem cell injection with anti-aging effects, which specifically includes the following steps:

[0061] Step 1. Resuscitate the qualified umbilical cord mesenchymal stem cells in a 37°C water bath for 2 minutes;

[0062] Step 2. Wash twice with clinical injection grade saline;

[0063] Step 3, dissolving 2wt% human serum albumin in the compound electrolyte solution;

[0064] Step 4, cell counting, maintain 95% viability, press 5×10 5 ~10×10 5 / ml resuspended in the complex solution of step 3.

[0065] The umbilical cord mesenchymal stem cell injection prepared by this method contains the following quantities or concentrations of components:

[0066] Umbilical cord mesenchymal stem cells: 5×10 5 ~10×10 5 / ml;

[0067] Human albumin: 2wt%;

[0068] Compound electrolyte solution: 98wt%.

Embodiment 3

[0070] This example relates to the animal experiment of the umbilical cord mesenchymal stem cell injection of the present invention infused into aging model rats:

[0071] Determination of anti-aging indicators includes: 1. Appearance characteristics: body weight, coat color, action, and activity; 2. Biochemical indicators: activity of superoxide dismutase (SOD), content of malondialdehyde (MDA), thymus and spleen IL-2, IL-10 content, liver and kidney ratio. Specific steps are as follows:

[0072] Step 1, aging model establishment and group comparison experiment;

[0073] Twenty-four SD rats aged 6-8 weeks were selected, and 8 were randomly divided into the normal group, and the aging model was established with the remaining 16 rats. The 8 rats in the normal group were subcutaneously injected with normal saline, once a day, for 8 consecutive weeks; the other 16 rats were injected with D-galactose 120mg / kg subcutaneously, once a day, for 8 consecutive weeks;

[0074] From th...

technical field

[0001] The invention relates to an umbilical cord mesenchymal stem cell injection with anti-aging effect and a preparation method thereof, belonging to the technical field of cell biology. Background technique

[0002] Stem cells are primitive cells with self-replication and multi-directional differentiation potential, and are the origin cells that form various tissues and organs of living organisms. Stem cell technology is one of the most important technologies in the field of life science and technology. It can derive cells into various types of cells and their tissues, resulting in some new therapeutic technologies, such as stem cell transplantation, tissue and organ reconstruction, and gene therapy. People can use their own or others' healthy stem cells and their derived tissues and organs to replace diseased or aging tissues and organs.

[0003] According to their developmental stage, stem cells can be divided into embryonic stem cells (Embryonic stem c...

Examples

Embodiment 1

[0050] This embodiment provides a method for extracting umbilical cord mesenchymal stem cells, which specifically includes the following steps:

[0051] Step 1. Screening and preservation of umbilical cords: screening and collecting umbilical cords of healthy full-term maternal volunteers, placing them in umbilical cord preservation solution at 4°C, and separating them within 24 hours; the umbilical cord preservation solution is a serum-free medium, without Contain antibiotics; the detection items of the screening include carrying virus, genetic family history, etc.; the viruses such as hepatitis B, hepatitis C, AIDS, syphilis, etc.;

[0052] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0053] Step 2. Separation of the umbilical cord: transfer the umbilical cord to a Petri dish and wash it with normal saline; remov...

Embodiment 2

[0060] This implementation provides a method for preparing an umbilical cord mesenchymal stem cell injection with anti-aging effects, which specifically includes the following steps:

[0061] Step 1. Resuscitate the qualified umbilical cord mesenchymal stem cells in a 37°C water bath for 2 minutes;

[0062] Step 2. Wash twice with clinical injection grade saline;

[0063] Step 3, dissolving 2wt% human serum albumin in the compound electrolyte solution;

[0064] Step 4, cell counting, maintain 95% viability, press 5×10 5 ~10×10 5 / ml resuspended in the complex solution of step 3.

[0065] The umbilical cord mesenchymal stem cell injection prepared by this method contains the following quantities or concentrations of components:

[0066] Umbilical cord mesenchymal stem cells: 5×10 5 ~10×10 5 / ml;

[0067] Human albumin: 2wt%;

[0068] Compound electrolyte solution: 98wt%.

Embodiment 3

[0070] This example relates to the animal experiment of the umbilical cord mesenchymal stem cell injection of the present invention infused into aging model rats:

[0071] Determination of anti-aging indicators includes: 1. Appearance characteristics: body weight, coat color, action, and activity; 2. Biochemical indicators: activity of superoxide dismutase (SOD), content of malondialdehyde (MDA), thymus and spleen IL-2, IL-10 content, liver and kidney ratio. Specific steps are as follows:

[0072] Step 1, aging model establishment and group comparison experiment;

[0073] Twenty-four SD rats aged 6-8 weeks were selected, and 8 were randomly divided into the normal group, and the aging model was established with the remaining 16 rats. The 8 rats in the normal group were subcutaneously injected with normal saline, once a day, for 8 consecutive weeks; the other 16 rats were injected with D-galactose 120mg / kg subcutaneously, once a day, for 8 consecutive weeks;

[0074] From th...

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