Storage solution capable of storing human mesenchymal stem cell at 4 DEG C

A technology of mesenchymal stem cells and storage solution, which is applied in the field of mesenchymal stem cell storage solution at 4°C, can solve the problems of unsatisfactory effect of mesenchymal stem cells, and achieve the effect of convenient clinical application

Inactive Publication Date: 2018-10-16
广东佰鸿干细胞再生医学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the technical defect that the mesenchymal stem cells are not ideally stored in the 4°C storage solution of the mesenchymal stem cells in the prior art

Method used

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  • Storage solution capable of storing human mesenchymal stem cell at 4 DEG C
  • Storage solution capable of storing human mesenchymal stem cell at 4 DEG C
  • Storage solution capable of storing human mesenchymal stem cell at 4 DEG C

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In order to overcome the technical defect of mesenchymal stem cell storage solution at 4°C in the prior art, the effect of storing mesenchymal stem cells is not satisfactory; the present invention provides a storage solution of mesenchymal stem cells at 4°C, the composition of the storage solution mainly includes: human blood Albumin, Trehalose, Sodium Chloride, Dextrose, Lactated Ringer's Injection, Heparin, L-Glutamine, EDTA, Phosphate Buffer, Sodium Selenite.

[0027] In this technical solution, the functions of the components of the mesenchymal stem cell storage solution at 4°C are as follows:

[0028] Human serum albumin: mainly regulates the dynamic balance of water in cells, maintains the osmotic pressure of cell solution, and is also a non-permeable cryoprotectant.

[0029] Trehalose: a stabilizer of cell biologically active substances, used for cell preservation, can effectively protect cell membranes and intracellular active proteins.

[0030] Sodium chloride...

Embodiment 2

[0042] The method for storing mesenchymal stem cells using a 4°C stock solution of mesenchymal stem cells comprises the following steps:

[0043] ⑴ Each component includes human albumin, trehalose, sodium chloride, glucose, sodium lactate Ringer injection, heparin, L-glutamine, EDTA, phosphate buffer, sodium selenite, prepared according to the concentration of the technical plan Mesenchymal stem cells stored at 4°C.

[0044] (2) Wash the digested mesenchymal stem cells 2-3 times with normal saline, count them, resuspend the cells in the above-mentioned mesenchymal stem cell storage solution at 4°C according to the cell concentration of 1-10E5 / mL, and aliquot them to 50mL transfer bags, 40mL per bag, and then heat-sealed to prepare a mesenchymal stem cell preparation.

[0045] (3) Store the prepared mesenchymal stem cell preparation in a 4°C refrigerator or a 4°C transport box, and use it within 48 hours.

[0046] (4) Warm the mesenchymal stem cell preparation to room tempera...

Embodiment 3

[0048] Further, the concentration of each component of the storage solution is specifically: human serum albumin is 1% mg / ml, sodium chloride is 0.9% ml / ml, trehalose is 50 mM, heparin is 1.33% IU / ml, L-glucose Aminoamide is 1 mM, EDTA is 0.05% mg / ml, phosphate buffer is 94.8% ml / ml, sodium selenite is 0.05% mg / ml, and compound amino acid is 5% ml / ml.

[0049] ⑴ Prepare mesenchymal stem cell storage solution at 4°C according to the concentration of each component human albumin, trehalose, sodium chloride, heparin, phosphate buffer, L-glutamine, EDTA, sodium selenite, and compound amino acids.

[0050] (2) Wash the digested mesenchymal stem cells 2-3 times with normal saline, count them, resuspend them with the above-mentioned mesenchymal stem cells at 4°C according to 1-10E5 / mL, and distribute them into 50mL transfer bags. The bag is 40mL, and then heat-sealed to prepare a mesenchymal stem cell preparation.

[0051] (3) Store the prepared mesenchymal stem cell preparation in ...

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Abstract

The invention relates to a storage solution capable of storing a human mesenchymal stem cell at 4 DEG C, the storage solution comprises the ingredients of human albumin, trehalose, sodium chloride, glucose, sodium lactate ringer's injection, heparin, L-glutamine, EDTA (Ethylene Diamine Tetraacetic Acid), a phosphate buffer solution, sodium selenite, an essential amino acid, and a non-essential amino acid. The provided formula guarantees that more than 80% of the vitality and more than 70% of the adhesion rate of the cell are kept after 48 hours in the storage and transportation, the requirement on the short-distance transportation of the fresh mesenchymal stem cell is met, the storage solution uses the clinical medicine having clear compositions, is safe to the human body, can be directlyinjected when recovered to the room temperature, and greatly facilitates the clinical application of the mesenchymal stem cell after the short-distance transportation.

Description

technical field [0001] The invention belongs to the field of mesenchymal stem cells, and in particular relates to a 4°C storage solution of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (human mesenchymal stem cells, hMSCs) are a type of stem cells with self-renewal, proliferation and multi-directional differentiation potential. They have a wide range of sources, are easy to collect, store and transport, have no allogeneic rejection, avoid ethical disputes, and stimulate tissue regeneration. , Regulating immune function and many other advantages. At present, the most used ones are mainly umbilical cord mesenchymal stem cells, fat mesenchymal stem cells, bone marrow mesenchymal stem cells, dental pulp mesenchymal stem cells, etc. [0003] Studies have shown that human mesenchymal stem cells have good therapeutic effects in cartilage regeneration, cerebral ischemic diseases, diabetes, rheumatoid arthritis and other animal models and clinical stu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 程蕊苹邓志宏
Owner 广东佰鸿干细胞再生医学有限公司
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