Storage solution capable of storing human mesenchymal stem cell at 4 DEG C
A technology of mesenchymal stem cells and storage solution, which is applied in the field of mesenchymal stem cell storage solution at 4°C, can solve the problems of unsatisfactory effect of mesenchymal stem cells, and achieve the effect of convenient clinical application
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Embodiment 1
[0026] In order to overcome the technical defect of mesenchymal stem cell storage solution at 4°C in the prior art, the effect of storing mesenchymal stem cells is not satisfactory; the present invention provides a storage solution of mesenchymal stem cells at 4°C, the composition of the storage solution mainly includes: human blood Albumin, Trehalose, Sodium Chloride, Dextrose, Lactated Ringer's Injection, Heparin, L-Glutamine, EDTA, Phosphate Buffer, Sodium Selenite.
[0027] In this technical solution, the functions of the components of the mesenchymal stem cell storage solution at 4°C are as follows:
[0028] Human serum albumin: mainly regulates the dynamic balance of water in cells, maintains the osmotic pressure of cell solution, and is also a non-permeable cryoprotectant.
[0029] Trehalose: a stabilizer of cell biologically active substances, used for cell preservation, can effectively protect cell membranes and intracellular active proteins.
Embodiment 2
[0042] The method for storing mesenchymal stem cells using a 4°C stock solution of mesenchymal stem cells comprises the following steps:
[0043] ⑴ Each component includes human albumin, trehalose, sodium chloride, glucose, sodium lactate Ringer injection, heparin, L-glutamine, EDTA, phosphate buffer, sodium selenite, prepared according to the concentration of the technical plan Mesenchymal stem cells stored at 4°C.
[0044] (2) Wash the digested mesenchymal stem cells 2-3 times with normal saline, count them, resuspend the cells in the above-mentioned mesenchymal stem cell storage solution at 4°C according to the cell concentration of 1-10E5 / mL, and aliquot them to 50mL transfer bags, 40mL per bag, and then heat-sealed to prepare a mesenchymal stem cell preparation.
[0045] (3) Store the prepared mesenchymal stem cell preparation in a 4°C refrigerator or a 4°C transport box, and use it within 48 hours.
[0046] (4) Warm the mesenchymal stem cell preparation to room tempera...
Embodiment 3
[0048] Further, the concentration of each component of the storage solution is specifically: human serum albumin is 1% mg / ml, sodium chloride is 0.9% ml / ml, trehalose is 50 mM, heparin is 1.33% IU / ml, L-glucose Aminoamide is 1 mM, EDTA is 0.05% mg / ml, phosphate buffer is 94.8% ml / ml, sodium selenite is 0.05% mg / ml, and compound amino acid is 5% ml / ml.
[0049] ⑴ Prepare mesenchymal stem cell storage solution at 4°C according to the concentration of each component human albumin, trehalose, sodium chloride, heparin, phosphate buffer, L-glutamine, EDTA, sodium selenite, and compound amino acids.
[0050] (2) Wash the digested mesenchymal stem cells 2-3 times with normal saline, count them, resuspend them with the above-mentioned mesenchymal stem cells at 4°C according to 1-10E5 / mL, and distribute them into 50mL transfer bags. The bag is 40mL, and then heat-sealed to prepare a mesenchymal stem cell preparation.
[0051] (3) Store the prepared mesenchymal stem cell preparation in ...
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