35 results about "Ringers solution" patented technology

The invention discloses a human umbilical cord mesenchymal stem cell (HUMSC) anti-hepatic fibrosis injection and a preparation method thereof. The injection is composed of 10%-50% of human albumin stock solution, 49.5%-89.5% of acetated Ringer's solution and 0.5% of heparin calcium, wherein, the concentration of the human albumin stock solution is 10%, and 1ml of the injection contains 6*10<5>-7*10<5> HUMSCs. The preparation method comprises the following steps: providing an MSC preserving fluid and pre-cooling the MSC preserving fluid for later use, wherein, the MSC preserving fluid comprises the human albumin stock solution, the Acetated Ringer's solution and the heparin calcium; providing the HUMSCs and adding the HUMSCs to the MSC preserving fluid; and adjusting the number of the HUMSCs added to the MSC preserving fluid to be 6*10<5>-7*10<5> HUMSCs/ml by means of re-suspending. The injection provided by the invention has the advantages of obvious curative effect and high stability, and is convenient in storage and transport and safe in use, thus being capable of bringing gospel to patients with hepatic fibrosis.

Tissue (10) from the colon or bowel is pretreated with a Ringers solution and mounted onto a ring (12) submerged in oxygenated Ringers solution (32), with first and second current electrodes (34,36) and first and second voltage electrodes (38,40) positioned within the solution (32) closely adjacent the tissue. A sequence of current pulses of different frequencies (44) covering a broad frequency range are applied via electrodes (34,36) to the tissue and corresponding impedance and resistance values are obtained (46). Comparison of impedance/resistance values establishes separate and distinct ranges of values for normal, cancerous and premalignant tissues.

The invention provides a novel nanometer lipid carrier containing a taxol substance for injection and a method for preparing the same. Mixed lipids are used as materials; liquid lipid and solid lipid with different physical states are mixed according to the proportion of between 0 and 100 percent to obtain the nanometer lipid carrier (NLC) which is prepared by a mixed solid-liquid substrate and has different crystalline states; and microemulsion (ME) prepared by a full liquid substrate and solid lipid nanoparticles (SLC) prepared by a full solid substrate are also obtained. The new nanometer lipid carrier can increase or improve the encapsulation efficiency and the release property of drug and has the characteristics of high drug-loading amount, good stability and low toxicity. The taxol substance, a carrier material, a surfactant and injection water are respectively stirred to form two phases of oil and water; the oil phase and the water phase are mixed and emulsified to obtain primary emulsion; further taxol substance nanometer lipid carrier injection or suspension type injection which meets the requirement of intravenous injection is prepared by a high-pressure homogenization process; or a protective agent is added into the injection to prepare solid powder for injection by vacuum freeze drying or spray drying; before use, normal saline, a glucose solution or a Ringer solution is used for dilution; and the solid powder can be rapidly dissolved. Experiments show that the prepared nanometer lipid carrier containing the taxol substance for injection can improve targeting property to tumor cells, improve curative effect, greatly reduce toxic and side effects, has simple preparation process and low cost and is suitable for large-scaled industrialized production.

An eyelid scrub composition comprising polyhexamethylene biguanide (PHMB), 1,2-hexanediol, 1,2-octanediol, and a pH stabilizing surfactant solution present in an amount effective to control the pH level of the composition between 5.5 and 7.5. The composition can further comprise moisturizers and foam stabilizers. The composition can be combined with a fabric pad for use as an eyelid scrub, where the fabric pad is pre-moistened with the composition and packaged for use. The composition may be applied to the eyelid scrub and rubbed to induce foaming. The composition is produced by preparing a modified Ringer's solution and adding 1,2-hexanediol, 1,2-octanediol, and an effective amount of one or more pH stabilizing surfactants. The mixture is then heated and allowed to cool before polyhexamethylene biguanide is added.

The invention relates to pelteobagrus fulvdraco sperm ultralow temperature freezing storage and recovery method. Parent pelteobagrus fulvdraco which is in good shape and good growth, has no disease and is sexually mature is selected, 30Mug/kg LHRH-A2 intraperitoneal injection is carried out to induce sperm increasing, after 36 hours, dissection is carried out to obtain spermary, and the spermary is cut into pieces in a clean mortar cooled in a refrigerator at 4 DEG C in advance, the pieces are diluted by Ringers solution in the proportion of 1:6, balancing is carried out for 30min at 4 DEG C, 10% methyl alcohol is added, and mixing to be uniform is carried out, packaging is carried out, balancing is carried out for 10min 6cm above liquid nitrogen surface, and balancing is carried out for 5min on the liquid nitrogen surface, and then the product is thrown into liquid nitrogen to be stored. Water bath ate 37 DEG C is carried out, activation solution SM is used for activation, pelteobagrus fulvdraco roe is extruded into a container, frozen sperm is added in drops in the sperm roe quantity ratio of 105:1, stirring to be uniform is carried out, then appropriate activation solution SM is added in drops, and stirring to be uniform is carried out, so that fertilization of roe is carried out. The sperm stored by the invention has high quality, has insemination capability and has important theoretical significance and application value on seed quality saving, inheritance breeding, sustainable culture and disease control of pelteobagrus fulvdraco.

The invention relates to the technology of chromosome operation, in particular to a method for inducing turbot homogenesis gynogenesis fry by using the theory that heredity inactivated sperms are used to perform artificial insemination and a fertilized ovum is restrained from cleavage through hydrostatic pressure. Sexually mature turbot parent fishes are selected, seminal fluid and spawn are collected respectively, insemination is performed on the seminal fluid and the spawn in a volume ratio of 1:100-200, and a control group is formed. The surplus seminal fluid is diluted by a precooled Ringer solution, and sperms are inactivated by ultraviolet rays. Insemination is performed on the inactivated seminal fluid and ova in a volume ratio of 1:10-20. In the 10-20 min before the first fertilized ovum crack appears, the fertilized ovum is placed in a hydrostatic press pressurizing vessel, and processing is performed by the hydrostatic pressure of 55-75 MPa for 6-8 min. Through the method, the 100% turbot homogenesis gynogenesis fry can be inducing obtained efficiently, stably and reliably.

The invention relates to the field of medicines, and particularly relates to an operation irrigating solution and a preparation method thereof. The irrigating solution comprises sodium hyaluronate and a solvent. The irrigating solution is characterized in that the solvent is normal saline, injection water, a ringer solution irrigating solution, a glucose irrigating solution, a mannitol irrigating solution, a chlorhexidine irrigating solution, a gentamycin normal saline irrigating solution, a povidone iodine irrigating solution, a bromogeramine irrigating solution, a glycine irrigating solution, a metronidazole irrigating solution or a physiological balanced solution; and the concentration of the sodium hyaluronate in the operation irrigating solution is 0.1-10g/L. The operation irrigating solution has the effects of operation irrigation, has functions of preventing adhesion and promoting wound healing after operation, and has the advantages of no toxic or side effect, good stability and excellent effect.

The invention discloses a method for preparing a functional artificial skin scaffold material. According to the method, gelatin having a relative molecular mass of over 10000 serving as a main base material, genipin serving as a crosslinking agent and deionized water, distilled water, normal saline, injection water or Ringer solution serving as a solvent are frozen and formed in a special forming mould by a temperature gradient formed in the vertical direction to obtain a porous material. According to the porous material having a skin biotic structure, a honeycomb porous structure is formed inside the material, the pore diameters are gradient from big to small from the lower surface to the upper surface, wherein the pore diameter of the upper surface is 5-70mu m, the pore diameter of the lower surface is 50-200mu m; adjacent holes are communicated. The preparation process is simple and easily controlled and has low manufacture cost; the prepared product has high and stable quality, has a skin simulation structure, and has excellent moisture absorption, biodegradability and biocompatibility.

The invention discloses a preparation method of a human skin simulation material with a three-dimensional gradient hole structure. The preparation method is characterized by taking sodium alginate or oxidized sodium alginate of which the relative molecular mass is more than 100,000 as a solute and deionized water, distilled water, normal saline, water for injection or ringer solution as a solvent, forming temperature gradient freezing molding in a dedicated forming die in the vertical direction, and then carrying out a cross-linking reaction, thus obtaining a porous material, wherein the inner part of the porous material is in a honeycomb shape, a plurality of numbers of holes are formed in the porous material, the pore diameter of each hole is respectively in gradient change from large to small from the lower surface to the upper surface, the pore diameter of the upper surface is 5 to 70 mum, the pore diameter of the lower surface of 50 to 200 mum, every two adjacent holes are communicated with each other, and the porous material has a skin simulation structure. According to the preparation method disclosed by the invention, the preparation technology is simple and is easy to control, the preparation cost is low, and a prepared product is good and stable in quality, has the skin simulation structure and has good water absorbing performance, good biodegradability and good biocompatibility.

A ganciclovir-sodium lactate liquid injection is proportionally prepared from ganciclovir or its derivative or its medicinal salt, sodium lactate, sodium chloride, potassium chloride, calcium chloride and the water for injection through dissolving carrier in the water for injection to obtain isotonic solution, dissolving ganciclovir in it, adding the water for injection, stirring, regulating pH=6.0-8.5, adding the water for injection, ultrafiltering to remove heat source, and high-pressure sterilizing.

The invention belongs to the field of biological medicines, and discloses a microcarrier culture system of umbilical cord mesenchymal stem cells (MSC), and preparation and applications of an MSC preserving fluid. The microcarrier culture system is characterized by preparing a heparin crosslinking sephadex microcarrier and a mating microcarrier culture medium, and a high-efficiency MSC culture system can be built. MSC cells can be efficiently and fast prepared within a short period by utilizing the microcarrier culture system of the MSC. The MSC preserving fluid is characterized by adding human albumin, heparin sodium, estrogen and IL-1beta into a ringer solution, the cell activity of the MSC cells can be kept unchanged within 24 hours by utilizing the preserving fluid, and the level of cell factors can be improved by about five times on average. The microcarrier culture system is applied to being developed into large-batch preparation of umbilical cord MSC for clinical use and laboratories, and provides a specific preserving method for MSC for treating systemic lupus erythematosus, and establishes a basis for the clinical application of the MSC.

The invention relates to an artificial induced spawning technology, in particular to an efficient loach artificial fertilization technology. The efficient loach artificial fertilization technology particularly comprises the following steps of determining the induced spawning date, determining the proportion of males and females, preparing and spraying medicine soup, carrying out prevention before induced spawning and injecting odinagogue, wherein the time is 15-20 hours, the water temperature is determined to range from 20 DEG C to 25 DEG C, loach is wrapped with a towel, a mixture of 0.1-0.2 milliliter is injected to each piece of loach, and the mixture includes 75% of chorionic gonadotropin and 25% of a Ringer solution. The efficient loach artificial fertilization technology has the advantages that the loach absorbs the medicine soup before induced spawning is carried out, so that the quality of seminal fluid and eggs is improved, the loach is better protected through a prevention mixture, the amount of survival young loach is large, yield is high, and the efficient loach artificial fertilization technology is unique, simple, good in effect and easy to popularize in family cultivation.

The invention discloses an instrument bag for fast building tissue engineering skin. The instrument bag comprises a bag body and surgical supplies arranged in the bag body, and the surgical supplies comprise digestive enzyme solution freeze-dried powder, decellularized allosome dermis stroma, a No.23 surgical knife blade, dressing forceps, an injector, an injector needle, a first sodium lactate Ringer solution, a second sodium lactate Ringer solution, a filter screen, sterile water and a micro heater. The instrument bag has the advantages of being fast, needing no cell subculturing, achieving one-time complement, being fast in built tissue engineering skin union and capable of restoring the function of the skin and the like.

The invention discloses a preparation of a platelet washing solution. The platelet washing solution comprises two solutions as follows: 500ml of a bicarbonate Ringer solution and 20-30ml of an ACD-A anticoagulant. The platelet washing solution prepared from 500ml of the bicarbonate Ringer solution and 25ml of the ACD-A anticoagulant is optimal in washing effect. According to the preparation of the platelet washing solution, two solutions with permission to the clinical application at present are mixed at a certain ratio; the platelet washing solution is used for washing and preparing plasma-poor platelet; by virtue of the clinical application of the plasma-poor platelet, the untoward effects of platelet transfusion caused by plasma proteins can be solved. The platelet washing solution is simple in components and liable to depolymerize; the recovery rate of the platelet is increased; the agglomeration force is improved; the safety is guaranteed.

The invention discloses Ringer's solution for animals. The composition and ratio of raw materials are as follows: VA with a content of 50%, VD3 with a content of 52.5%, VK3 with a content of 0.96%, VB1 with a content of 1%, and a content of 0.8 % VB2, 0.98% VB6, 0.01% VB12, 0.98% L‑VC, 0.98% Niacin, 0.98% Folic Acid, 0.8% Calcium Pantothenate, The content is 0.02% biotin, 0.01% sodium selenite, NaCl, KCl, sodium citrate, no fruit sugar, and the weight ratio is disclosed. The invention also discloses a preparation method. It solves the problems of increasing animal sub-health groups, body fluid imbalance and electrolyte disorder, serious imbalance of yin and yang, and limited productivity.

A process for preparing bioceramics beta-TCP with flaky cleavage grain structure includes proportionally mixing bihydrated calcium hydrogen phosphate with calcium carbonate, heating to 930 deg.C, holding the temp for 3 hr, suddenly cooling in liquid nitrogen, suddenly heating to 930 deg.C, natural cooling, immersing in original Ringer solution at 37 deg.C for 7 days, and drying. The said bioceramics can be easily degradated in human body.

The invention discloses a preparation method of a loach oxytocic injection. The loach oxytocic injection comprises the following components: 30 micrograms of DOM, 300 IU of chorionic gonadotropin, and 5 micrograms of luteinzing-hormone releasing hormone, for each loach. The preparation method comprises the following steps: mixing the components mentioned above, and dissolving the mixture in a Ringer's solution so as to obtain the loach oxytocic injection, wherein the Ringer's solution is prepared by the following steps: evenly mixing 7.5 grams of NaCl, 0.2 gram of KCl, and 0.4 gram of CaCl, and then dissolving the mixture in a sterilized water solution with a volume of 1 L so as to obtain the Ringer's solution. The preparation method is simple and easy to master. The effect of the oxytocic injection is more prominent than that of single components and other combinations.