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Pelteobagrus fulvdraco sperm ultralow temperature freezing and recovery technology

A technology for ultra-low temperature freezing and yellow catfish, which can be used in fish farming, climate change adaptation, and preservation of human or animal bodies. It can solve the problems of quality damage, pesticide pollution, etc. Good effect with little damage

Inactive Publication Date: 2010-12-08
FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE +1
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

In recent years, the phenomenon of overfishing in the Yangtze River waters has been serious, and the level of pesticide pollution has increased, which has damaged its quality to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] Ultra-low temperature cryopreservation and resuscitation methods and techniques of yellow catfish sperm:

[0009] The choice of broodstock: the original species or improved species with normal shape, good growth, no disease, and reaching sexual maturity.

[0010] Collection of gametes: During the breeding period of yellow catfish, 30 μg / kg LHRH-A 2 The sperm was induced by intraperitoneal injection. After 36 hours, the testes were dissected and cut into pieces in a clean mortar pre-cooled in a refrigerator at 4°C.

[0011] Vitality detection of fresh semen: First, suck a drop of water on the glass slide with a thin-mouthed straw, aim at the field of view, then pick up the above-mentioned semen with a needle tip and immediately observe its vitality while stirring evenly under the microscope.

[0012] Freezing method: After diluting the semen and Ren's solution by 1:6, equilibrate at 4°C for 30 minutes, add 10% methanol to mix, subpackage, equilibrate at 6 cm above the l...

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PUM

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Abstract

The invention relates to pelteobagrus fulvdraco sperm ultralow temperature freezing storage and recovery method. Parent pelteobagrus fulvdraco which is in good shape and good growth, has no disease and is sexually mature is selected, 30Mug / kg LHRH-A2 intraperitoneal injection is carried out to induce sperm increasing, after 36 hours, dissection is carried out to obtain spermary, and the spermary is cut into pieces in a clean mortar cooled in a refrigerator at 4 DEG C in advance, the pieces are diluted by Ringers solution in the proportion of 1:6, balancing is carried out for 30min at 4 DEG C, 10% methyl alcohol is added, and mixing to be uniform is carried out, packaging is carried out, balancing is carried out for 10min 6cm above liquid nitrogen surface, and balancing is carried out for 5min on the liquid nitrogen surface, and then the product is thrown into liquid nitrogen to be stored. Water bath ate 37 DEG C is carried out, activation solution SM is used for activation, pelteobagrus fulvdraco roe is extruded into a container, frozen sperm is added in drops in the sperm roe quantity ratio of 105:1, stirring to be uniform is carried out, then appropriate activation solution SM is added in drops, and stirring to be uniform is carried out, so that fertilization of roe is carried out. The sperm stored by the invention has high quality, has insemination capability and has important theoretical significance and application value on seed quality saving, inheritance breeding, sustainable culture and disease control of pelteobagrus fulvdraco.

Description

technical field [0001] The invention relates to ultra-low temperature cryopreservation and resuscitation methods of yellow catfish sperm Background technique [0002] Yellow catfish (Pelteobagrus fulvidraco), commonly known as thorny fish, yellow aunt, yellow curd, etc., is an important economic fish in the Yangtze River Basin. Because of its tender meat, delicious taste, rich nutrition and high economic value, it is deeply loved by people and has become one of the emerging freshwater aquaculture species in China in recent years. In recent years, overfishing in the waters of the Yangtze River has become serious, and the level of pesticide pollution has increased, which has damaged its quality to a certain extent. Therefore, the research on the cryopreservation technology of its sperm is of great significance to the protection of germplasm resources of peltundra catfish. Contents of the invention [0003] The technical problem to be solved by the present invention is to p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02A01K61/00
CPCY02A40/81
Inventor 葛家春丁淑燕黄亚红严维辉潘建林李跃华郝忱赵沐子许志强
Owner FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
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