Preparation method of human skin simulation material with three-dimensional gradient hole structure

Abstract

The invention discloses a preparation method of a human skin simulation material with a three-dimensional gradient hole structure. The preparation method is characterized by taking sodium alginate or oxidized sodium alginate of which the relative molecular mass is more than 100,000 as a solute and deionized water, distilled water, normal saline, water for injection or ringer solution as a solvent, forming temperature gradient freezing molding in a dedicated forming die in the vertical direction, and then carrying out a cross-linking reaction, thus obtaining a porous material, wherein the inner part of the porous material is in a honeycomb shape, a plurality of numbers of holes are formed in the porous material, the pore diameter of each hole is respectively in gradient change from large to small from the lower surface to the upper surface, the pore diameter of the upper surface is 5 to 70 mum, the pore diameter of the lower surface of 50 to 200 mum, every two adjacent holes are communicated with each other, and the porous material has a skin simulation structure. According to the preparation method disclosed by the invention, the preparation technology is simple and is easy to control, the preparation cost is low, and a prepared product is good and stable in quality, has the skin simulation structure and has good water absorbing performance, good biodegradability and good biocompatibility.

Description

technical field [0001] The invention relates to a preparation method of a medical porous material, in particular to a preparation method of a human skin simulation material with a three-dimensional gradient pore structure. Background technique [0002] The choice of biomaterial determines the biocompatibility of the constructed porous scaffold material. Sodium alginate is a natural material extracted from seaweed plants, and is one of the natural biomaterials approved by the US Food and Drug Administration (FDA) for use in medical fields such as tissue engineering. [0003] Sodium alginate, a polysaccharide, has a structure similar to that of the skin dermal matrix component: aminoglycan, and has good biocompatibility. Skin fibroblasts, liver cells, chondrocytes, and osteoblasts are easily absorbed in alginate porous materials. It survives and forms an extracellular matrix. At the same time, sodium alginate also has good film-forming, gelling, hygroscopicity, and barrier ba...

AI Technical Summary

Problems solved by technology

Since the temperature conduction in the low-temperature refrigerator is along the axis of the pre-freezing model, the pore diameters of porous materials are different along this direction, but the pore size of the longitudinal section is the same. Preparation and performance research of calcium acid-based interpenetrating network membrane materials" studied the alginate solution with a concentration of 2% and pre-frozen in a low-temperature refrigerator at -5°C. The pores in the longitudinal section were uniform and the pore diameter was 100-300 μm. , but due to the single pore size, it is not suitable for the cultivation of full-thickness skin, and it is easy to cause scars when used clinically

Studies have shown that gradient tissue engineering scaffolds with biomimetic skin structures are more conducive to skin regeneration. For skin tissue engineering scaffolds with biomimetic skin structures, research reports are mostly prepared by double-layer or multi-layer composite methods or other methods. It is more time-consuming. For example, Harley and Oh et al. studied the use of rotation / centrifugation technology combined with freeze-drying technology to construct a porous scaffold with a gradient pore structure in the radial direction. The pore size of the scaffold can be adjusted by the rotation speed, but this technology is generally only applicable to the preparation of blood vessels. Tubular scaffold materials are not suitable for constructing other scaffold materials (Harley, B.A., Hastings, A.Z., Yannas, I.V. & Sannino, A. Fabricatingtubular scaffoldswith hara radial pore size gradient by as pinning technique. Biomaterials27,866-874, doi:10.1016 / j.biomaterials.2005.07.06) ; Oh, S.H., Park, I.K., Kim, J.M. & Lee, J.H. Invitro and in vivo characteristics of PCL scaffold with pores size gradient fabricated by acentrifugation method. Biomaterials 28, 1664-1671, doi: 10.1016 / j. biomaterials. The pore size distribution is controlled by adjusting the size of the porogen, but the porogen is difficult to completely remove, and the residual porogen is not good for the later use of the material (Wu, H. et al. .Fabricationofchitosan-g-polycaprolactonecopolymerscaffoldswithgradientporousmicrostructures.MaterialsLetters62,2733-2736,doi:10.1016 / j.matlet.2008.01.029(2008);Zhang,Q.,Lu,H.,Kawazoe,N.&Chen,G.Preparationofcollagenporousscaffoldswithagradientporesizestructureusingiceparticulates.MaterialsLetters107, 280-283, doi: 10.101 6 / j.matlet.2013.05.070(2013); Mao, J.S., Zhao, L.G., Yin, Y.J. & Yao, K.D. Mao et al. placed the sample in a unidirectional heat conduction environment to prepare a double-layer scaffold material. Due to the single pre-freezing temperature, the pore size of the scaffold formed cannot be adjusted, and no gradient pore structure is formed. TanyaJ.Levingstone et al. used layer-by-layer self- The assembly method constructs three-layer gradient bionic cartilage scaffolds, and each layer of scaffolds is prepared by freeze-drying. The preparation of a cartilage scaffold requires three freeze-drying processes, which is time-consuming and laborious (Levingstone, T.J., Matsiko, A., Dickson, G.R., O'Brien, F.J. & Gleeson, J.P. Abiomimetic multi-layered collagen-based scaffold for osteochondral repair. ActaBiomaterialia10, 1996-2004, doi: 10.1016 / j.actbio.2014.01.005(2014))