A method for inducing turbot homogeneous gynogenetic fry

A technology of gynogenesis and turbot, which is applied in the field of chromosome manipulation, can solve the problems of diploid induction and restricted application of homogeneous turbot homogeneous gynogenesis, so as to avoid recovery, improve survival rate, and avoid waste Effect

Active Publication Date: 2017-02-22
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the optimal treatment conditions of different fish species are often quite different, the homogeneous gynogenesis induction conditions of the above fish species cannot be used to successfully induce homogeneous gynogenetic diploids in turbot, which restricts its application.

Method used

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  • A method for inducing turbot homogeneous gynogenetic fry

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In the fertilization period, incubation period and induction period of this embodiment, the seawater temperature used in the three periods is a constant water temperature suitable for fertilization and hatching of turbot eggs, that is, the seawater temperature in the three periods of the fertilization period, incubation period and induction period is 14.5 ± 0.1°C.

[0025] 1) Collection of gametes

[0026] Select broodstock that are in the process of laying eggs and expecting to lay them gently on the cushion, wipe off the water and dirt around their body surface and reproductive holes, and then slowly squeeze from back to front, and squeeze the sperm and eggs into the beaker respectively Or reserve in a basin. Observed under the dissecting microscope, the eggs are spherical, transparent, oil globules are concentrated, the egg diameter is moderate, and the size is uniform; the semen is milky white and the concentration is appropriate.

[0027] 2) Artificial inseminati...

Embodiment 2

[0043] In the fertilization period, incubation period and induction period of this embodiment, the seawater temperature used in the three periods is a constant water temperature suitable for the fertilization and hatching of turbot eggs, that is, the seawater temperature in the three periods of the fertilization period, the incubation period and the induction period is 15.0 ± 0.1°C.

[0044] 1) Collection of gametes

[0045] Select broodstock that are in the process of laying eggs and expecting to lay them gently on the cushion, wipe off the water and dirt around their body surface and reproductive holes, and then slowly squeeze from back to front, and squeeze the sperm and eggs into the beaker respectively Or reserve in a basin. Observed under an anatomical microscope, the eggs are spherical, transparent, with concentrated oil globules, moderate egg diameter, and uniform size; Swimming quickly, unable to see the trajectory of movement), swimming for a long time.

[0046] 2...

Embodiment 3

[0062] In the fertilization period, incubation period and induction period of this embodiment, the seawater temperature used in the three periods is a constant water temperature suitable for fertilization and hatching of turbot eggs, that is, the seawater temperature in the three periods of the fertilization period, incubation period and induction period is 15.5 ± 0.1°C.

[0063] 1) Collection of gametes

[0064] Select broodstock that are in the process of laying eggs and expecting to lay them gently on the cushion, wipe off the water and dirt around their body surface and reproductive holes, and then slowly squeeze from back to front, and squeeze the sperm and eggs into the beaker respectively Or reserve in a basin. Under the dissecting microscope, the eggs were spherical, transparent, with concentrated oil globules, moderate in diameter and uniform in size; the semen was milky white with appropriate concentration, and the motility of the sperm was grade IV after being acti...

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Abstract

The invention relates to the technology of chromosome operation, in particular to a method for inducing turbot homogenesis gynogenesis fry by using the theory that heredity inactivated sperms are used to perform artificial insemination and a fertilized ovum is restrained from cleavage through hydrostatic pressure. Sexually mature turbot parent fishes are selected, seminal fluid and spawn are collected respectively, insemination is performed on the seminal fluid and the spawn in a volume ratio of 1:100-200, and a control group is formed. The surplus seminal fluid is diluted by a precooled Ringer solution, and sperms are inactivated by ultraviolet rays. Insemination is performed on the inactivated seminal fluid and ova in a volume ratio of 1:10-20. In the 10-20 min before the first fertilized ovum crack appears, the fertilized ovum is placed in a hydrostatic press pressurizing vessel, and processing is performed by the hydrostatic pressure of 55-75 MPa for 6-8 min. Through the method, the 100% turbot homogenesis gynogenesis fry can be inducing obtained efficiently, stably and reliably.

Description

technical field [0001] The invention relates to chromosome manipulation technology, in particular to a method for inducing turbot homogeneous gynogenetic fry. Background technique [0002] Turbot (Scophthalmus maximus) belongs to the family Scophthalmidae and the genus Scophthalmus. It grows fast and has developed very rapidly since it was introduced from Europe in 1992. It has become the main marine aquaculture species in northern my country. And the industrial chain is also relatively complete, with a total annual output of more than 100,000 tons, forming a large-scale industry. However, in recent years, the turbot breeding industry has seriously degraded its germplasm due to generations of breeding and inbreeding. Therefore, it is urgent to adopt effective methods to genetically improve turbot and obtain improved varieties. Hybridization is one of the most effective methods of breed improvement. Varieties with excellent traits can be obtained by crossing different strai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10
CPCA01K61/00Y02A40/81
Inventor 吴志昊尤锋宋宗诚王丽娟范兆飞曹元水
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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