Umbilical cord mesenchymal stem cell injection as well as preparation method and application thereof

A stem cell and injection technology, applied in the field of biomedicine, can solve the problems of unclear pathogenesis of inflammatory bowel disease, reduced or ineffective drug response, serious drug toxicity and side effects, etc. The effect of increasing the rate and reducing the toxic and side effects

Inactive Publication Date: 2015-09-02
北京青藤谷禧干细胞科技研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The disadvantages of the current technology are: because the pathogenesis of inflammatory bowel disease is not clear at present, there is no specific drug, and the course of the disease is long, and it is easy to relapse. Heal and relieve symptoms such as diarrhea and blood in the stool
Diet control, hormone therapy, salicylic acid drugs, and sulfasalazine therapy can play a certain role in controlling the disease, but at the same time, the toxic and side effects of the drug are also very serious, and long-term use is likely to cause a decrease in drug response or ineffectiveness , It also greatly reduces the quality of life for the patient itself; surgical treatment can have a certain therapeutic effect, but it is highly selective for patients, and it carries certain surgical risks, postoperative sequelae, such as severe bleeding, intestinal obstruction , intestinal perforation, fistula formation, ulceration and canceration, etc., the risk further increases

Method used

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  • Umbilical cord mesenchymal stem cell injection as well as preparation method and application thereof
  • Umbilical cord mesenchymal stem cell injection as well as preparation method and application thereof
  • Umbilical cord mesenchymal stem cell injection as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] In this example, the umbilical cord mesenchymal stem cells were isolated and cultured by the following method, and the specific steps were as follows:

[0077] Take fresh umbilical cords that have left the mother’s body for no more than 2 hours after cesarean section, and check whether the mother is carrying infectious disease pathogens (hepatitis B surface antigen, hepatitis C antibody, syphilis antibody, AIDS antibody, negative), and place the fresh umbilical cord containing D-Hanks balance Stored in a special storage bottle for the liquid, transported in a low-temperature ice box;

[0078] The biosafety cabinet was irradiated with ultraviolet rays for 30 minutes, and the water bath shaker was preheated at 37°C in advance, and the umbilical cord sample bottle was placed in the biosafety cabinet for operation. Take out the sample with long hemostatic forceps and place it in a 150mm sterile glass dish, and wash away the blood on the surface of the umbilical cord with D-...

Embodiment 2

[0089] In this example, the umbilical cord mesenchymal stem cell injection is prepared by the following method, the injection contains mesenchymal stem cells and the cryopreservation solution, wherein the cryopreservation solution contains the following components:

[0090] Bomaili A injection with a volume percentage of 44%;

[0091] 6% hydroxyethyl starch 130 / 0.4 with a mass volume percentage in g / mL;

[0092] Adenosine triphosphate disodium magnesium chloride with a mass volume percentage of 10% in g / mL;

[0093] 10% clinical grade DMSO by volume;

[0094] 20% human albumin at 30% by volume.

[0095] Prepared by the following method, specifically comprising the following steps:

[0096] (1) Bomaili A injection (Shanghai Baite Medical Supplies Co., Ltd.), hydroxyethyl starch 130 / 0.4 (Qidu Pharmaceutical), adenosine triphosphate disodium magnesium chloride (Yabao Pharmaceutical Group Co., Ltd.) and 20 % Human serum albumin (Shanghai Raas Blood Products Co., Ltd.) was mixe...

Embodiment 3

[0114] In this example, the umbilical cord mesenchymal stem cell injection is prepared by the following method, the injection contains mesenchymal stem cells and the cryopreservation solution, wherein the cryopreservation solution contains the following components:

[0115] Volume percentage content is 40% Naringer acetate injection;

[0116] 6% hydroxyethyl starch 130 / 0.4 with a mass volume percentage in g / mL;

[0117] Adenosine triphosphate disodium magnesium chloride with a mass volume percentage of 10% in g / mL;

[0118] 10% clinical grade DMSO by volume;

[0119] 20% human albumin at 34% by volume.

[0120] Prepared by the following method, specifically comprising the following steps:

[0121] (1) Mix Nalinger Acetate Injection (Hunan Kangyuan Pharmaceutical Co., Ltd.), hydroxyethyl starch 130 / 0.4, adenosine triphosphate disodium magnesium chloride and 20% human serum albumin according to the above volume percentage, and use a 0.22 μm bacterial filter filter, reserve; ...

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Abstract

The invention provides umbilical cord mesenchymal stem cell injection as well as a preparation method and application thereof. The umbilical cord mesenchymal stem cell injection comprises mesenchymal stem cells and a frozen stock solution, wherein the frozen stock solution comprises the following components: a balanced electrolyte solution with the volume percentage of 25-70 percent, hydroxyethyl starch 130/0.4 with the mass/volume percentage (g/ml) of 1-10 percent, triphosadenine disodium magnesium chloride with the volume percentage of 5-20 percent, clinical-grade DMSO with the volume percentage of 5-20% and 20% human albumin with the volume percentage of 1-50 percent. The prepared umbilical cord mesenchymal stem cell injection is free of animal serum, safe, high in cell viability after cryopreservation resuscitation, can be used for treating inflammatory bowel disease, has a good effect and has no toxic or side effects.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to an umbilical cord mesenchymal stem cell injection and a preparation method and application thereof. Background technique [0002] Inflammatory bowel disease (Inflammatory Bowel Disease, IBD) is a group of idiopathic, chronic, inflammatory bowel disease states that can cause irreversible damage to intestinal structure and function, including two main disease types: Crohn's disease ( Crohn's Disease, CD) and ulcerative colitis (Ulcerative Colitis, UC). The pathogenesis of Crohn's disease is complex and still unclear. The clinical features of Crohn's disease include diarrhea, abdominal pain, intestinal stricture and intestinal obstruction due to narrowing of the intestinal lumen, abscess formation, fistula formation, and may be accompanied by intestinal inflammatory manifestations such as joints, eyes, skin, mouth, and liver. Ulcerative colitis is a non-specific inflammation of unknown cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/08A61K47/42A61P37/02A61P7/00A61P1/00
Inventor 孙磊孙萍宋亚昆马世泽史振生吴东颖
Owner 北京青藤谷禧干细胞科技研究院有限公司
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