4 results about "Hepatic stem cell" patented technology

The invention provides a support system for preserving an in-vitro liver through bidirectional driving of a liver and stem cell tank, which comprises an organ preserving device, a first circulating pipeline and a second circulating pipeline, and the first circulating pipeline and the second circulating pipeline are mutually independent; the organ storage device is used for storing the in-vitro liver; the first circulating pipeline is provided with a power part and a circulating purification unit, one end of the first circulating pipeline is connected with the inferior vena cava of the in-vitro liver in the organ storage device, and the other end of the first circulating pipeline is connected with the portal vein of the in-vitro liver; the second circulating pipeline is provided with a circulating pump and a bioreactor inoculated with mesenchymal stem cells, one end of the second circulating pipeline is connected with the inferior vena cava of the in-vitro liver in the organ preservation device, and the other end of the second circulating pipeline is connected with the hepatic artery of the in-vitro liver. The in-vitro liver is perfused through two channels, the purposes of detoxifying and supplementing essential factors can be achieved at the same time, and the in-vitro preservation time of the in-vitro liver can be effectively prolonged.

The invention relates to an exosome-containing composition, which is prepared by blending a modified silicon dioxide agent and an exosome matrix according to a weight ratio of (2-5): 15, the exosome matrix is composed of a mesenchymal stem cell source exosome and a liver stem cell source exosome, and the mass ratio of the mesenchymal stem cell source exosome to the liver stem cell source exosome is 1: (0.5-2). The exosome is a core functional component which plays a role in treating hepatic failure, the biological activity of the exosome is greatly improved through double-source selection and hypoxia pretreatment, the double-stem-cell exosome has a synergistic effect, proliferation and repair of damaged hepatic cells can be directly promoted, and liver functional cells can be supplemented; through compounding of the two exosomes, triple synergy of inhibiting necrosis, promoting regeneration and regulating immunity can be realized, and the problems of single function and limited repair efficiency of a single exosome are solved.

The invention discloses a method for three-dimensional multiplication culture of a hepatic stem cell line. The method comprises the following steps: inoculating cells of the liver stem cell line into a low-adsorption culture plate containing a HepSC culture medium, and culturing at the rotating speed of 100-180 rpm to enable the cells to form cell spheres. According to the invention, the dependence of the HepSC cell line on mouse matrigel and adherent culture is effectively solved, a new three-dimensional culture way without matrigel is provided for large-scale amplification, and clinical transformation of the liver stem cell line can be promoted.

According to the present invention, methods are provided for inducing liver stem cells or liver progenitor cells from non-liver stem cells or non-liver progenitor cells, methods for manufacturing liver stem cells or liver progenitor cells, and methods for manufacturing hepatocytes or bile duct epithelial cells.