6 results about "Hepatic stem cell" patented technology

The invention discloses application of Rab8a in preparation of drugs for treating or preventing or improving individual hepatic fibrosis, and belongs to the technical field of drugs and disease treatment. The invention provides application of Rab8a in preparation of a medicine for treating or preventing or improving individual hepatic fibrosis. The Rab8a can enhance autophagy by down-regulating the expression level of the I-type TGF-beta receptor on the HSCs membrane, so that the activation of the HSCs is reduced, the hepatic fibrosis is favorably retarded or improved, and the Rab8a has the potential of serving as a target spot for treating the hepatic fibrosis.

The invention provides a support system for preserving an in-vitro liver through bidirectional driving of a liver and stem cell tank, which comprises an organ preserving device, a first circulating pipeline and a second circulating pipeline, and the first circulating pipeline and the second circulating pipeline are mutually independent; the organ storage device is used for storing the in-vitro liver; the first circulating pipeline is provided with a power part and a circulating purification unit, one end of the first circulating pipeline is connected with the inferior vena cava of the in-vitro liver in the organ storage device, and the other end of the first circulating pipeline is connected with the portal vein of the in-vitro liver; the second circulating pipeline is provided with a circulating pump and a bioreactor inoculated with mesenchymal stem cells, one end of the second circulating pipeline is connected with the inferior vena cava of the in-vitro liver in the organ preservation device, and the other end of the second circulating pipeline is connected with the hepatic artery of the in-vitro liver. The in-vitro liver is perfused through two channels, the purposes of detoxifying and supplementing essential factors can be achieved at the same time, and the in-vitro preservation time of the in-vitro liver can be effectively prolonged.

The invention relates to an exosome-containing composition, which is prepared by blending a modified silicon dioxide agent and an exosome matrix according to a weight ratio of (2-5): 15, the exosome matrix is composed of a mesenchymal stem cell source exosome and a liver stem cell source exosome, and the mass ratio of the mesenchymal stem cell source exosome to the liver stem cell source exosome is 1: (0.5-2). The exosome is a core functional component which plays a role in treating hepatic failure, the biological activity of the exosome is greatly improved through double-source selection and hypoxia pretreatment, the double-stem-cell exosome has a synergistic effect, proliferation and repair of damaged hepatic cells can be directly promoted, and liver functional cells can be supplemented; through compounding of the two exosomes, triple synergy of inhibiting necrosis, promoting regeneration and regulating immunity can be realized, and the problems of single function and limited repair efficiency of a single exosome are solved.

The invention relates to the technical field of cell culture, in particular to hepatic stem cells obtained by directional induced differentiation of mesenchymal stem cells as well as a preparation method and application of the hepatic stem cells. The induction method comprises the following steps: (1) inoculating mesenchymal stem cells into an induction culture medium, and culturing for 5-7 days to obtain intermediate cells 1; (2) inoculating the intermediate cell 1 into a differential medium, and culturing for 3-6 days to obtain an intermediate cell 2; and (3) inoculating the intermediate cell 2 into a mature culture medium, culturing for 3-6 days, filtering, centrifuging, collecting precipitate, and drying to obtain the liver stem cell. According to the method disclosed by the invention, the mesenchymal stem cells can be directionally converted into the liver stem cells. The liver stem cell obtained by the invention can be used for treating liver cirrhosis, has a good curative effect and no side effect, and provides a basis for treating liver cirrhosis.

The invention discloses a method for three-dimensional multiplication culture of a hepatic stem cell line. The method comprises the following steps: inoculating cells of the liver stem cell line into a low-adsorption culture plate containing a HepSC culture medium, and culturing at the rotating speed of 100-180 rpm to enable the cells to form cell spheres. According to the invention, the dependence of the HepSC cell line on mouse matrigel and adherent culture is effectively solved, a new three-dimensional culture way without matrigel is provided for large-scale amplification, and clinical transformation of the liver stem cell line can be promoted.

According to the present invention, methods are provided for inducing liver stem cells or liver progenitor cells from non-liver stem cells or non-liver progenitor cells, methods for manufacturing liver stem cells or liver progenitor cells, and methods for manufacturing hepatocytes or bile duct epithelial cells.