Artificial polypeptide capable of inducing bone mesenchymal stem cells to differentiate into hepatic cells and biological product of such artificial polypeptide

A technology of bone marrow mesenchyme and biological products, applied in the field of stem cells, which can solve the problems such as the inability to maintain the original characteristics and the lack of cell sources.

Active Publication Date: 2017-08-18
诺赛联合(北京)生物医学科技有限公司
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Problems solved by technology

[0002] Liver cell transplantation is one of the most effective methods for the treatment of end-stage liver failure, but its clinical application is affected by factors such as lack of cell sources, difficulty in mass proliferation in vitro, failure to maintain its original characteristics after passage, and immune rejection.

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  • Artificial polypeptide capable of inducing bone mesenchymal stem cells to differentiate into hepatic cells and biological product of such artificial polypeptide

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Embodiment Construction

[0026] The substantive content of the present invention will be specifically introduced below in conjunction with the embodiments. Due to space reasons, the description of the experimental process cannot be done in great detail. All the parts not described in detail in the experiment are routine operations well known to those skilled in the art.

[0027] 1. Experimental materials

[0028] The polypeptide involved in the present invention is synthesized by the standard polypeptide solid-phase synthesis technique well known in the art, using the N-terminal protection strategy of fluorenylmethoxycarbonyl (Fmoc). According to the method of resin solid-phase synthesis, the corresponding amino acids are sequentially connected, during which the Fmoc-protecting group is sequentially removed, and then the peptide is cut to obtain a crude product. The crude product is separated and purified by a C18 column to obtain the polypeptide represented by formula (IV).

[0029] HPLC and MS detec...

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Abstract

The invention discloses an artificial polypeptide capable of inducing bone mesenchymal stem cells to differentiate into hepatic cells and a biological product of such artificial polypeptide. The artificial polypeptide is a polypeptide containing an amino acid sequence shown in formula (IV): LGENQPDAK(Xa)PCFQEDPMA(Xb)GTDCTLMEIWN, (IV), wherein each of Xa and Xb is selected from M, Y, L, V, W or E. Professionals in the field know that a strong ability to induce the bone mesenchymal stem cells to differentiate into the hepatic cells is achieved in case of combination of acidic fibroblast growth factor, hepatocyte growth factor and oncostatin M, and after induced culture for 14 days, alpha fetal protein mRNA and albumin mRNA in the cells are in remarkably positive expression. It is proven that the artificial polypeptide with the same effect is capable of inducing the bone mesenchymal stem cells to differentiate into the hepatic cells, and accordingly the artificial polypeptide can be prepared into the biological product for inducing the bone mesenchymal stem cells to differentiate into the hepatic cells.

Description

technical field [0001] The invention belongs to the field of stem cells, and relates to directional induced differentiation of stem cells, in particular to an artificial polypeptide for inducing bone marrow mesenchymal stem cells to differentiate into liver cells and biological products thereof. Background technique [0002] Hepatocyte transplantation is one of the most effective methods for the treatment of end-stage liver failure, but its clinical application is affected by factors such as lack of cell sources, difficulty in mass proliferation in vitro, failure to maintain its original characteristics after passage, and immune rejection. With the progress of tissue engineering research, stem cell therapy for end-stage liver disease has become the most promising development direction. [0003] Bone marrow mesenchymal stem cells are a kind of non-hematopoietic stem cells first reported and discovered by Friedenstein et al. in the early 1970s. Because these cells are spindle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00C12N5/071
CPCC07K14/00C12N5/067C12N2501/998C12N2506/1353
Inventor 王莉李岩孙天娇徐梦龙胡芳
Owner 诺赛联合(北京)生物医学科技有限公司
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